Endemic Light

10.26686/wgtn.17065223 ◽

2021 ◽

Author(s):

◽

Sarah Tyrrell

Keyword(s):

Theoretical Research ◽

Abstract Concepts ◽

Site Specific ◽

Public Building ◽

Interior Spaces ◽

Mackenzie Basin ◽

Research Through Design ◽

Town Centre ◽

Building Form ◽

Steven Holl

This thesis explores endemic light and atmosphere through the shifting scales of three architectural interventions. These interventions are guided by site and theoretical research, providing justification for the notion of endemic light. This notion develops upon the concept of site specific architecture and place. It is the synthesis of site context – combining both ephemeral and phenomenological qualities to create engaging and evocative architectural experiences. Analysis of the Mackenzie Basin site established an overarching understanding of the atmospheric, physical, social and historical contexts of the area. Peter Zumthor, Steven Holl, Juhani Pallasmaa and Christian Norberg-Schulz provide key justifications for the theoretical investigation of light, atmosphere, and place; as well as ongoing precedence for the research through design process. This process explores three interventions, moving up in scale from an installation, to a domestic dwelling, and finally a public building. The installation operates at an interactive scale, exploring abstract concepts of condensing light within a space, through manipulation of light, colour and texture. The domestic scale expands on this research, developing condensed light and atmosphere at a habitable scale. Through designing for light and atmosphere the dwelling becomes a device for endemic atmospheric experiences in a domestic context. The final scale explores a public building in the form of a town centre for Twizel. This intervention adapts the notion of condensing light within interior spaces, instead exploring at an urban scale, intensifying them externally through courtyards and exterior building form. The thesis concludes, that successful and immersive architectural experiences are generated through strong ephemeral and phenomenological connections and engagement with site and endemic light.

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Boden Black (A Novel) and With Axe and Pen in the New Zealand Alps: Differences Between Overseas and New Zealand Written Accounts of Climbing Mount Cook 1882-1920 and the Emergence of a New Zealand Voice in Mountaineering Literature

10.26686/wgtn.17000845 ◽

2021 ◽

Author(s):

◽

Jura Fearnley

Keyword(s):

New Zealand ◽

Family Members ◽

Southern Alps ◽

Critical Study ◽

First Person ◽

The Novel ◽

Writing Style ◽

Natural Landscape ◽

Site Specific ◽

Mackenzie Basin

This thesis has two components: creative and critical. The creative component is the novel Boden Black. It is a first person narrative, imagined as a memoir, and traces the life of its protagonist, Boden Black, from his childhood in the late 1930s to adulthood in the present day. The plot describes various significant encounters in the narrator’s life: from his introduction to the Mackenzie Basin and the Mount Cook region in the South Island of New Zealand, through to meetings with mountaineers and ‘lost’ family members. Throughout his journey from child to butcher to poet, Boden searches for ways to describe his response to the natural landscape. The critical study is titled With Axe and Pen in the New Zealand Alps. It examines the published writing of overseas and New Zealand mountaineers climbing at Aoraki/Mount Cook between 1882 and 1920. I advance the theory that there are stylistic differences between the writing of overseas and New Zealand mountaineers and that the beginning of a distinct New Zealand mountaineering voice can be traced back to the first accounts written by New Zealand mountaineers attempting to reach the summit of Aoraki/Mount Cook. The first mountaineer to attempt to climb Aoraki/Mount Cook was William Spotswood Green, an Irishman who introduced high alpine climbing to New Zealand in 1882. Early New Zealand mountaineers initially emulated the conventions of British mountaineering literature as exemplified by Green and other famous British mountaineers. These pioneering New Zealand mountaineers attempted to impose the language of the ‘civilised’ European alpine-world on to the ‘uncivilised’ world of the Southern Alps. However, as New Zealand mountaineering became more established at Aoraki/Mount Cook from the 1890s through to 1920, a distinct New Zealand voice developed in mountaineering literature: one that is marked by a sense of connection to place expressed through site-specific, factual observation and an unadorned, sometimes laconic, vernacular writing style.

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An Evaluation of Measuring the Publicness Level of Interiors in Public Building Design

Human-Computer Interaction and Technology Integration in Modern Society - Advances in Human and Social Aspects of Technology ◽

10.4018/978-1-7998-5849-2.ch012 ◽

2021 ◽

pp. 276-303

Author(s):

Pelin Aykutlar ◽

Seçkin Kutucu ◽

Işın Can-Traunmüller

Keyword(s):

Building Design ◽

Design Stage ◽

Public Buildings ◽

Public Building ◽

Early Design ◽

Early Design Stage ◽

The Public ◽

Interior Spaces ◽

Floor Plans ◽

Architectural Competitions

This study examines the publicness level of the interior spaces of public buildings. As a method, VGA (visual graph analysis) is used for analyzing the early design phases of selected municipal service buildings. In this study, the authors utilized from VGA for quantifying the publicness level of the two selected architectural competitions of municipality buildings. The method allows us analyzing the floor plans of each project in obtaining an eventual assessment of permeability and accessibility which give an idea of the levels of publicness comparatively. Subsequently, representation parameters are compared under two main criteria: connectivity and integration. The aim of the study is to understand the level of publicness and efficiency of spatial settings for the users circulating in the public buildings, which have dissimilar plan schemes. This method would be used by the designers for early design stage and provide useful feedback for understanding the level of accessibility and permeability of the structures and adjust their schemes accordingly.

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Boden Black (A Novel) and With Axe and Pen in the New Zealand Alps: Differences Between Overseas and New Zealand Written Accounts of Climbing Mount Cook 1882-1920 and the Emergence of a New Zealand Voice in Mountaineering Literature

10.26686/wgtn.17000845.v1 ◽

2021 ◽

Author(s):

◽

Jura Fearnley

Keyword(s):

New Zealand ◽

Family Members ◽

Southern Alps ◽

Critical Study ◽

First Person ◽

The Novel ◽

Writing Style ◽

Natural Landscape ◽

Site Specific ◽

Mackenzie Basin

This thesis has two components: creative and critical. The creative component is the novel Boden Black. It is a first person narrative, imagined as a memoir, and traces the life of its protagonist, Boden Black, from his childhood in the late 1930s to adulthood in the present day. The plot describes various significant encounters in the narrator’s life: from his introduction to the Mackenzie Basin and the Mount Cook region in the South Island of New Zealand, through to meetings with mountaineers and ‘lost’ family members. Throughout his journey from child to butcher to poet, Boden searches for ways to describe his response to the natural landscape. The critical study is titled With Axe and Pen in the New Zealand Alps. It examines the published writing of overseas and New Zealand mountaineers climbing at Aoraki/Mount Cook between 1882 and 1920. I advance the theory that there are stylistic differences between the writing of overseas and New Zealand mountaineers and that the beginning of a distinct New Zealand mountaineering voice can be traced back to the first accounts written by New Zealand mountaineers attempting to reach the summit of Aoraki/Mount Cook. The first mountaineer to attempt to climb Aoraki/Mount Cook was William Spotswood Green, an Irishman who introduced high alpine climbing to New Zealand in 1882. Early New Zealand mountaineers initially emulated the conventions of British mountaineering literature as exemplified by Green and other famous British mountaineers. These pioneering New Zealand mountaineers attempted to impose the language of the ‘civilised’ European alpine-world on to the ‘uncivilised’ world of the Southern Alps. However, as New Zealand mountaineering became more established at Aoraki/Mount Cook from the 1890s through to 1920, a distinct New Zealand voice developed in mountaineering literature: one that is marked by a sense of connection to place expressed through site-specific, factual observation and an unadorned, sometimes laconic, vernacular writing style.

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From the sound installation to the sound situation: on my work transition – berlin junction eine klangsituation

Organised Sound ◽

10.1017/s1355771803000098 ◽

2003 ◽

Vol 8 (2) ◽

pp. 187-193 ◽

Cited By ~ 1

Author(s):

GEORG KLEIN

Keyword(s):

Public Spaces ◽

Site Specificity ◽

Narrow Sense ◽

Foreign Material ◽

Site Specific ◽

Sound Installation ◽

Musical Space ◽

Interior Spaces ◽

Work Transition ◽

Expanded Concept

Since mid-1999 I have been working on an expanded concept of musical space not only incorporating space according to its spatial characteristics in the narrow sense (acoustic, architectonic, sculptural, perspective and ambient). Beyond this my installation works conceive of space in its site-specificity, usually existing in several layers: a social layer, a historical-political layer and a situational layer. This concept of space is thus not limited to interior spaces and architectonic-formal relationships, a position which naturally also defines my working methods: only subjective, on-site research yields the theme and concept of an installation. I call such installations sound situations, following the ‘conscious creation of situations’ conceived by Guy Debord (International Situationists) in the 1950s. This means first that I open myself up to situations – usually in public spaces – in order to sense their site-specific tensions and to draw them out in all their possible relationships. Secondly, I alter these situations in order to make them more dense through intensification. The concept can be defined as articulating the space, the site; for this reason I prefer to work with site-specific acoustic and visual material found at the site, but also introduce foreign material when it serves to reinforce communication with the site and its occupants and visitors.

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Large-cluster and combined fluorescent and gold immunoprobes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166920 ◽

1996 ◽

Vol 54 ◽

pp. 892-893

Author(s):

Richard D. Powell ◽

James F. Hainfeld ◽

Carol M. R. Halsey ◽

David L. Spector ◽

Shelley Kaurin ◽

...

Keyword(s):

Metal Cluster ◽

Sulfonyl Chloride ◽

Gel Filtration ◽

Cross Linking ◽

Site Specific ◽

Fab Fragments ◽

Cluster Label ◽

Covalently Linked ◽

Texas Red ◽

Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

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The challenge of detecting modifications on proteins

Essays in Biochemistry ◽

10.1042/ebc20190055 ◽

2020 ◽

Vol 64 (1) ◽

pp. 135-153 ◽

Cited By ~ 1

Author(s):

Lauren Elizabeth Smith ◽

Adelina Rogowska-Wrzesinska

Keyword(s):

Mass Spectrometry ◽

Protein Function ◽

Chemical Properties ◽

Lysine Acetylation ◽

Mass Determination ◽

Post Translational Modifications ◽

Site Specific ◽

The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

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