scholarly journals FEATURES OF CLINICAL AND LABORATORY PARAMETERS AT THE PATIENS WITH RECURRENT ORAL AFTS AND CONDOMITANT ACID-DEPENDENT DISEASES OF THE GASTROINTESTINAL TRACT

2020 ◽  
Vol 16 (3) ◽  
pp. 23-29
Author(s):  
Irina Galimova ◽  
Rita Yunusova ◽  
Irina Usmanova ◽  
Larisa Gerasimova ◽  
Yigal Granot ◽  
...  
Keyword(s):  
Free Radical ◽  
Gastrointestinal Tract ◽  
Oral Cavity ◽  
Oral Hygiene ◽  
Oral Fluid ◽  
Free Radical Oxidation ◽  
The State ◽  
Radical Oxidation ◽  
Laboratory Parameters ◽  
Litmus Paper

Subject. Some features of the clinical picture, hygienic and microbiological state of the oral cavity, acidity and free-radical oxidation of the oral fluid in patients with recurrent aphthates on the background of acid-dependent diseases of the gastrointestinal tract are considered. The aim is to assess the relationship between clinical and laboratory parameters in patients with recurrent oral aphthae associated with acid-dependent diseases of the gastrointestinal tract. Methodology. A comprehensive clinical and laboratory examination of 58 young people with revealed recurrent aphthae of the oral cavity against the background of acid-dependent diseases of the gastrointestinal tract was carried out. The level of oral hygiene was assessed using the ONI-s index, the state of free-radical oxidation of the oral fluid - the "Chemilumimer-003" device, individual representatives of the normal flora were identified by inoculating the contents from the surface of aphthous elements on special differential diagnostic media of the HiCrome series. The acidity of the oral fluid was determined using a universal indicator litmus paper pH-1-14, short-term intragastric pHmetry was performed directly during fibrogastroduodenoscopy. Results. In 46.4 % of those examined, recurrent aphthae were found on the oral mucosa. In persons with chronic gastritis and duodenitis, the frequency of recurrences once a year was 37.9 %, 2-3 times a year ― 62.0, with the presence of chronic pancreatitis against the background of hyperacid gastric secretion ― 44.8 and 55.2, respectively. In patients with recurrent aphthae, a low level of oral hygiene, a violation of its microbiome, and a change in the acidity of the oral fluid were revealed. Conclusions. The presence of recurrent aphthae complicates individual oral hygiene, because accompanied by severe pain and paresthetic symptoms. This is manifested by an increase in the ONI-S index and a disturbance in the state of the normobiome in the form of an increase in the growth of resident microflora against the background of changes in the acidity of the oral fluid. Chemiluminescence indices correlate with the hygienic state of the oral cavity, qualitative changes in the normobiota and pH of the oral fluid in the studied clinical groups.

The state of free radical oxidation in the tissue of primary vulvar cancer and of its recurrence.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e22234-e22234
Author(s):  
Ekaterina Igorevna Surikova ◽  
Irina Aleksandrovna Goroshinskaya ◽  
Galina Andreevna Nerodo ◽  
Ekaterina Alekseevna Nerodo ◽  
Elena Vladimirovna Shalashnaya ◽  
...  
Keyword(s):  
Free Radical ◽  
Vulvar Cancer ◽  
Free Radical Oxidation ◽  
The State ◽  
Radical Oxidation

scholarly journals EFFICIENCY OF THE COMPLEX OF THERAPEUTIC ACTIVITIES OF CHRONIC GYNGIVITIS IN PATIENTS WITH CHRONIC GASTRODUODENITE

2018 ◽  
Vol 14 (2) ◽  
pp. 25-29
Author(s):  
Миляуша Кабирова ◽  
Milyausha Kabirova ◽  
Лариса Герасимова ◽  
Larisa Gerasimova ◽  
Сафия Каримова ◽  
...  
Keyword(s):  
Free Radical ◽  
Light Emission ◽  
Free Radical Oxidation ◽  
Positive Control ◽  
Negative Control ◽  
The State ◽  
Radical Oxidation ◽  
Oral Liquid ◽  
Primary 52 ◽  
Therapeutic Activities

Subject. The article presents clinical, biochemical, immunological and microbiological results of a complex of therapeutic measures of chronic gingivitis in patients with chronic gastroduodenitis. The aim of the study was to study the effectiveness of treatment of chronic gingivitis in patients with chronic gastroduodenitis with the use of the proposed method. Methodology. The subject of the study was 155 persons aged 19 to 25 years with diagnosed chronic gingivitis and chronic gastroduodenitis, which were divided by stratified randomization into three groups: 1 group (primary) - 52 patients; 2 group (positive control) - 52 patients; Group 3 (negative control) - 51 patients. All groups were comparable in age (F = 2,355, p = 0,092) and sex (χ2 = 2,133, p = 0,334). To study the state of free radical oxidation, the method of chemiluminescence (CL) of the oral liquid was applied on the device HL-003. The quantitative and qualitative content of oral microflora was determined using nutrient media: CHROMagar Orientation, CHROMagar Candida (France). The concentration in the oral fluid of the intercellular adhesion molecules sICAM-1 was carried out on the automatic enzyme immunoassay analyzer Lazurit and test systems manufactured by Vector-Best CJSC (Russia) and eBioscience (Austria). Results. The use of the complex proposed by us significantly improved the parameters of the clinical examination (dental indices). In addition, the state of free-radical oxidation has normalized: at previously increased values of the light-emission sum (S) and its maximum value (I max), during the time of the study, their decrease occurred, while the lowered values - the increase to the level of normal values. The level of microorganisms of the orange and red complex decreased to normal levels, and the level of sICAM-1 also significantly decreased. Conclusions. The scheme of treatment of chronic gingivitis with the use of NUTRIEL with laminaria and tooth elixir with chlorophyll in patients with chronic gastroduodenitis led to an increase in the duration of remission compared with the traditional treatment regimen.

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

scholarly journals PECULIARITIES OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION PARAMETERS OF THE ORAL FLUID IN CHILDREN WITH CHRONIC CATARRHAL GINGIVITIS WITH UNDERLYING DIABETES MELLITUS

2021 ◽  
Vol 74 (4) ◽  
pp. 887-890
Author(s):  
Liudmyla F. Kaskova ◽  
Valentyna A. Honcharenko ◽  
Oksana V. Klitynska
Keyword(s):  
Diabetes Mellitus ◽  
Free Radical ◽  
Oral Fluid ◽  
Lipid Peroxide ◽  
Free Radical Oxidation ◽  
Antioxidant Protection ◽  
State Of Health ◽  
General State ◽  
Peroxide Oxidation ◽  
Radical Oxidation

The aim: Objective of the research was to study peculiarities of free radical oxidation and antioxidant protection parameters of the oral fluid in children with chronic catarrhal gingivitis with underlying diabetes mellitus. Materials and methods: Oral fluid was examined in 105 children aged 12 including somatically healthy ones with intact periodontium and chronic catarrhal gingivitis, suffering from diabetes mellitus up to 5 years and more than 5 years with chronic catarrhal gingivitis. The parameters of lipid peroxide oxidation (level of diene conjugates, Malone dialdehyde) and antioxidant protection (activity of SOD and catalase, POM, whole protein; ceruloplasmin; НS-groups) of the oral fluid were determined. Results: The results obtained are indicative of available probable difference in the examined parameters among children depending on their general state of health and periodontal tissue condition. The best parameters were found among somatically healthy children with intact periodontium. They deteriorated in children with chronic catarrhal gingivitis available irrespective of their general state of health. They reached the most critical level in children with diabetes mellitus lasting longer than 5 years. Conclusions: The course of chronic catarrhal gingivitis in children, both somatically healthy and those suffering from diabetes mellitus, is associated with increased parameters of lipid peroxide oxidation and decreased enzymatic activity of the antioxidant protection system of the oral fluid. The most considerable changes are found in patients suffering from diabetes mellitus lasting more than 5 years.

scholarly journals CHARACTERISTICS OF CYTOKINE PROFILE OF ORAL FLUID IN PATIENTS WITH CHRONIC SIMPLE MARGINAL GINGIVITIS

2021 ◽  
Vol 11 (3) ◽  
pp. 75-79
Author(s):  
Adel Isaeva ◽  
Sergey Averyanov ◽  
Ilgiz Iskhakov ◽  
Shamil Galimov ◽  
Elmira Galimova ◽  
...  
Keyword(s):  
Free Radical ◽  
Oral Fluid ◽  
Periodontal Diseases ◽  
Free Radical Oxidation ◽  
Mechanisms Of Action ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Periodontal Tissues ◽  
Inflammatory Processes ◽  
Effective Drugs

Biomarkers of inflammation can be used as indicators for monitoring the treatment of periodontal diseases, as well as for finding the safest and most effective drugs with different mechanisms of action. The concentration of biomarkers in saliva was determined by enzyme immunoassay and chemiluminescent analysis. The obtained results reflect the degree of activity of inflammatory processes in periodontal tissues that occur in gingivitis and reveal an imbalance in the processes of free radical oxidation and antioxidant protection of the oral cavity.

scholarly journals Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes

2021 ◽  
Vol 11 (3) ◽  
pp. 12-17
Author(s):  
A. V. Elikov
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Former Athletes ◽  
Oxidative Balance

Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent.

scholarly journals Microbiocenoses and the State of Local Mucosal Immunity of the Oral Cavity of Children and Adolescents with Liver Diseases

2021 ◽  
Vol 6 (5) ◽  
pp. 316-321
Author(s):  
O. V. Khmil ◽  
◽  
D. O. Khmil ◽  
L. F. Kaskova ◽  
O. V. Silkova ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Children And Adolescents ◽  
Oral Hygiene ◽  
Mucosal Immunity ◽  
Liver Diseases ◽  
Oral Fluid ◽  
The State ◽  
Chronic Liver ◽  
Chronic Liver Diseases ◽  
Local Immunity

The purpose of the study was to study the relationship between the state of the oral cavity microbiocenosis and the level of local immunity in children and adolescents with chronic liver diseases. Materials and methods. 45 children aged 10 to 16 years, who were treated in the public utility "City Children's Clinical Dental Hospital of the Poltava City Council" were examined. All children were divided into 2 groups: the main group – children and adolescents with chronic hepatitis (n = 26) with diseases of concomitant oral mucosa and periodontal disease; control group – children of the same age without concomitant somatic pathology (n = 20). In all observation groups, the state of oral hygiene was recorded using the simplified oral hygiene index (Green-Vermillion, 1964) and the intensity of the inflammatory process in the periodontal tissues, which was assessed by the papillary-marginal-alveolar index modified by Parma (1960). The degree of dysbiosis in the oral cavity was determined using the enzymatic method of A. P. Levitsky by the ratio of the relative activity of urease and lysozyme. The state of local immunity was investigated by the level of immunoglobulins IgA, IgM, IgG and secretory immunoglobulin sIgA. Results and discussion. It was found that the majority of patients with chronic hepatitis had an unsatisfactory and even poor state of oral hygiene, according to the Green-Vermillion simplified oral hygiene index, in combination with moderate and severe gingivitis (according to the papillary-marginal-alveolar index). The most negative results were registered in children 9-13 years old. The effect of the low level of oral hygiene in children and adolescents with chronic liver diseases, which is accompanied by increased dysbiosis, was studied, which indicates a drop in the level of antimicrobial protection, suppression of local mucosal immunity, a decrease in lysozyme activity and a decrease in the content of secretory sIgA in the oral fluid. The mediated mechanism of sIgA action through the phagocyte activation system with subsequent lysis of pathogenic microorganisms is considered. Conclusion. Chronic liver diseases reliably lead to changes in the body's immunobiological reactivity, and also cause suppression of the protective mechanisms of the oral cavity and its local immunity, characterized by a decrease in the activity of lysozyme and secretory IgA in the oral fluid of sick children and adolescents

scholarly journals Correction of the state of antioxidant protection system of the fowl’s organism under the circumstances of being poisoned by Derosal

2020 ◽  
Vol 22 (98) ◽  
pp. 46-50
Author(s):  
O. S. Kochevenko ◽  
I. O. Zhukova ◽  
O. M. Bobrytska ◽  
I. O. Kostiuk ◽  
L. A. Vodopyanova
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
The Body ◽  
Superoxide Dismutase Activity ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Active Forms Of Oxygen ◽  
Radical Oxidation

In fact, Free radical oxidation is one of the required factors of homeostasis as far as the active forms of oxygen, which have unpaired electron, perform useful functions, they are necessary intermediaries of numerous processes of normal cells’ functioning such as biosynthesis of prostaglandins and leukotriene as well as other biologically active substances. Also, they take part in pathological processes such as: inflammation, fever and others. Free radical oxidation is the process of direct oxygen transferring to the substrate forming peroxide, ketones, aldehydes, etc. Some amino acids, proteins, water and carbohydrates prone to the processes of peroxidation, but crucial place is granted to lipids at the expanse of fatty acids which are included to their composition. The article highlights the information concerning the analysis of the state of antioxidant system of hens in the process of feeding them with subtoxic doses of fungicide of Derosal (BAYER, Germany) (0.1 LD50 for a fowl, 900 mg/kg of the body mass) which active ingredient is benzimidazole carbamate (carbendazim, BMC). It presents the changes of these indicators in the process of using pharmacological and natural antioxidants. The analysis has figured out that giving Derosal to the hens without corrections (II group) has been followed with the activation of the process of lipids’ peroxidation (POL) in the blood plasma, that is followed with the increase of concentration of primary and secondary lipoperoxidation products – diene conjugates (DC) and TBA-active products on the average up to 21.0–25.0 %, catalase activity – up to 38.5 % and decrease of superoxide dismutase activity (SOD) up to 21.8 % and АОА – up to 17.8 %, that shows the adverse effects of pesticides on the system of antioxidant protection of the organism. In the fowls’ groups III, IV and V the adding of antioxidant E-selenium, ascorbic acid and thermally untreated buckwheat seeds as the source of  quercetins and anthocyanins has led to the induction of antioxidant resources that is shown by the decrease in comparison with the control of content of diene conjugates, malonic dialdehyde and catalase (in average up to 13.6–33.6–18.0 % accordingly) and compensatory strengthening of superoxide dismutase activity and general activity of antioxidant (АОА) on the average up to 44.9–72.1–76.5 % and 15.3–13.4–21.7 % accordingly. In comparison with group II the activity of catalase has been lower as well as the activity of superoxide dismutase has been higher in all the groups almost in 1.5–2 times (Р ≤ 0.001).

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