scholarly journals PECULIARITIES OF FREE RADICAL OXIDATION AND ANTIOXIDANT PROTECTION PARAMETERS OF THE ORAL FLUID IN CHILDREN WITH CHRONIC CATARRHAL GINGIVITIS WITH UNDERLYING DIABETES MELLITUS

2021 ◽  
Vol 74 (4) ◽  
pp. 887-890
Author(s):  
Liudmyla F. Kaskova ◽  
Valentyna A. Honcharenko ◽  
Oksana V. Klitynska
Keyword(s):  
Diabetes Mellitus ◽  
Free Radical ◽  
Oral Fluid ◽  
Lipid Peroxide ◽  
Free Radical Oxidation ◽  
Antioxidant Protection ◽  
State Of Health ◽  
General State ◽  
Peroxide Oxidation ◽  
Radical Oxidation

The aim: Objective of the research was to study peculiarities of free radical oxidation and antioxidant protection parameters of the oral fluid in children with chronic catarrhal gingivitis with underlying diabetes mellitus. Materials and methods: Oral fluid was examined in 105 children aged 12 including somatically healthy ones with intact periodontium and chronic catarrhal gingivitis, suffering from diabetes mellitus up to 5 years and more than 5 years with chronic catarrhal gingivitis. The parameters of lipid peroxide oxidation (level of diene conjugates, Malone dialdehyde) and antioxidant protection (activity of SOD and catalase, POM, whole protein; ceruloplasmin; НS-groups) of the oral fluid were determined. Results: The results obtained are indicative of available probable difference in the examined parameters among children depending on their general state of health and periodontal tissue condition. The best parameters were found among somatically healthy children with intact periodontium. They deteriorated in children with chronic catarrhal gingivitis available irrespective of their general state of health. They reached the most critical level in children with diabetes mellitus lasting longer than 5 years. Conclusions: The course of chronic catarrhal gingivitis in children, both somatically healthy and those suffering from diabetes mellitus, is associated with increased parameters of lipid peroxide oxidation and decreased enzymatic activity of the antioxidant protection system of the oral fluid. The most considerable changes are found in patients suffering from diabetes mellitus lasting more than 5 years.

scholarly journals CHARACTERISTICS OF CYTOKINE PROFILE OF ORAL FLUID IN PATIENTS WITH CHRONIC SIMPLE MARGINAL GINGIVITIS

2021 ◽  
Vol 11 (3) ◽  
pp. 75-79
Author(s):  
Adel Isaeva ◽  
Sergey Averyanov ◽  
Ilgiz Iskhakov ◽  
Shamil Galimov ◽  
Elmira Galimova ◽  
...  
Keyword(s):  
Free Radical ◽  
Oral Fluid ◽  
Periodontal Diseases ◽  
Free Radical Oxidation ◽  
Mechanisms Of Action ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Periodontal Tissues ◽  
Inflammatory Processes ◽  
Effective Drugs

Biomarkers of inflammation can be used as indicators for monitoring the treatment of periodontal diseases, as well as for finding the safest and most effective drugs with different mechanisms of action. The concentration of biomarkers in saliva was determined by enzyme immunoassay and chemiluminescent analysis. The obtained results reflect the degree of activity of inflammatory processes in periodontal tissues that occur in gingivitis and reveal an imbalance in the processes of free radical oxidation and antioxidant protection of the oral cavity.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

scholarly journals Genetic peculiarities of free radical oxidation of lipids and proteins in the semen of breeding boars

Biologija ◽  
2018 ◽  
Vol 64 (3) ◽  
Author(s):  
Svitlana Polishchuk ◽  
Svitlana Tsekhmistrenko ◽  
Vitalii Polishchuk ◽  
Oksana Tsekhmistrenko ◽  
Nataliia Ponomarenko ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Reproductive Function ◽  
Oxidative Modification ◽  
Peroxide Oxidation ◽  
Large White ◽  
Radical Oxidation ◽  
Key Enzyme ◽  
Synthetic Line

Complex research into biochemical aspects of the functioning of the antioxidant system for the protection of sperm of the breeding boars has established that the intensity of the flow of peroxide oxidation of lipids in germ cells is much higher than in the extracellular space. Reactions of free radical peroxide oxidation of proteins and lipids in the semen of healthy breeding boars are characterized by a stable level of activity that is necessary for the normal course of implementation of processes of the reproductive function. All components of the antioxidant system stay in mutual compensatory ratios under physiological conditions. As a rule, reducing the concentration or activity of some antioxidant enzymes leads to corresponding changes in others. The main antioxidants of the germ cell genomes are superoxide dismutase and ceruloplasmin. Catalase is a key enzyme that neutralizes H2O2 in the semen fluid of breeding boars. The content of total proteins in the semen of the synthetic line SS23 animals is greater (p < 0.05) than in the breeding boars of the large white breed. The processes of the oxidative modification of proteins in the animals body of the synthetic line runs more intensely, as it is evidenced by the higher content of the aldehyde and ketodinitrophenyl hydrazones of the main and neutral character in the sperm cytoplasm.

scholarly journals ACETAMINOPHEN EFFECT ON FREE RADICAL OXIDATION INDICES IN RATS WITH TYPE 2 DIABETES MELLITUS

2017 ◽  
Author(s):  
O. B. Furka ◽  
I. B. Ivanusa ◽  
M. M. Mykhalkiv ◽  
I. M. Klishch
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Free Radical ◽  
Liver Homogenate ◽  
Free Radical Oxidation ◽  
Maximum Increase ◽  
Radical Oxidation ◽  
Age Related

Background. Acetaminophen is a drug used to relieve pain syndrome. It is used both independently and in composition of combined drugs. Type 2 diabetes is an age-related disease that is associated with a violation of insulin synthesis by pancreas.Objective. The aim of the research was to study the effect of acetaminophen on major free radical oxidation indices of rats with type 2 diabetes mellitus in time dynamics.Methods. We conducted two series of experiments. The first series comprised rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions. The second series involved rats with type 2 diabetes mellitus and acetaminophen administration at a dose of 55 mg/kg for the period of 7 days.Results. Administration of acetaminophen for rats with type 2 diabetes mellitus caused the increase in the content of malondialdehyde, diene and triene conjugates and Schiff bases in blood plasma and malondialdehyde, diene and triene conjugates in liver homogenate. The maximum increase in these indices was observed on the first day of the experiment. Gradually these indices decreased on the 3rd, 5th and 7th days of the experiment.Conclusions. Free radical oxidation increased in both series of the experiment. This process developed in rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions more intensively, than in rats with type 2 diabetes mellitus and administration of acetaminophen at the highest therapeutic dose during 7 days.

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

scholarly journals INVESTIGATING EFFECTS OF 7,8-DISUBSTITUTED DERIVATIVE OF THEOPHYLLINE (BENOPHYLLINE) ON FREE-RADICAL OXIDATION IN RENAL INJURY OF RATS

2020 ◽  
Vol 20 (1) ◽  
pp. 29-35
Author(s):  
A. P. Matviiychuk ◽  
R. F. Yeremenko ◽  
A. V. Matviiychuk ◽  
A. V. Taran ◽  
O. M. Hladchenko
Keyword(s):  
Free Radical ◽  
Renal Injury ◽  
Water Solution ◽  
Rat Kidney ◽  
Free Radical Oxidation ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Disubstituted Derivative ◽  
Test Kit ◽  
The Mean

This article highlights the pharmacological study of new 7,8-disubstituted derivative of theophylline, the substance conditionally named benophylline. Based on the literature data and previous studies of compounds of this class, it has been concluded that studying the effect of benophylline on the pro-antioxidant system under simulated pathologies is of great interest and importance.  The study of antihypoxic action of 7,8-disubstituted theophylline in rats was carried out under modelled normobaric hypoxia with hypercapnia (in an enclosed space). It has been found out the application of 7-n-methylbenzyl-8-substituted polyphosphonates increases the longevity of rats in the conditions described above that evidences the improvement of metabolic processes in mitochondrial respiratory chain. Based on the results of screening studies, we selected 7,8-disubstituted derivative of theophylline (benophylline) as a leading substance for further investigation. The purpose of the study was to evaluate the efficacy of benophylline for the pharmacological correction of free radical processes in experimental renal damage in rats based on the evaluation of the expression of lipid peroxidation and antioxidant protection. Materials and methods: blood serum, homogenate and rat kidney tissues were the study materials. The study of the protective effect of benophylline on the functional state of the kidneys was investigated on experimental model of acute renal injury by introducing 50% water solution of glycerol (myoglobin nephropathy). Pathogenesis of this model was described in the methodological recommendations by C. Yu. Shtrygol, V. M. Lysovoi, I. A. Zupanetz et al. in «Methods of experimental modeling of kidney damage for pharmacological research». The content of TBK-R was determined spectrophotometrically by reaction with thiobarbituric acid. Glutathione recovered activity was determined spectrophotometrically in reaction with Elman's reagent using the method of Beutler E. D. et al. The catalase activity was assessed by the method of M.A. Koroluk et al. The influence of benophylline on the morphological state of the renal parenchyma was assessed on the modeled myoglobin nephropathy. The activity of gamma-glutamyltranspeptidase in serum was evaluated photometrically using a reagent test kit (“Filisit”). Statistical processing of the results was performed using the STATISTICA 8.0 software package, with the mean value, standard error of the mean confidence interval (p≤0.05). The microscopic study of microproducts was performed under the Granum microscope. Micrographs were taken with a Granum DMC 310 digital camera. The images were processed on a Pentium 2.4 GHz computer using Toup View software. Research results: Under the influence of benophylline, the processes of LPO and AOP became normalized, the expressiveness of degradation of the nephrocytes decreased, the microscopic picture of renal excretion in all rats got normalized. At the same time, 62.5% of the rats had the morphological condition of kidneys close to the intact control, and 37.5% of the animals were noticed to demonstrate a significant decrease in the dyscirculatory abnormalities, inflammatory manifestations and signs of nephrocyte regeneration. Conclusions: The ability of benophylline to inhibit the processes of free radical oxidation and to enhance antioxidant protection has been found out. The study has shown benophylline enhances regenerative effect kidney tissues.

The level of free radical oxidation products in heart and blood plasma by diabetes mellitus with chronic alcohol intoxication

2010 ◽  
Vol 56 (2) ◽  
pp. 257-265
Author(s):  
A.V. Indutny ◽  
D.E. Bykov ◽  
V.E. Vysokogorsky
Keyword(s):  
Diabetes Mellitus ◽  
Free Radical ◽  
Blood Plasma ◽  
Alcohol Intoxication ◽  
Free Radical Oxidation ◽  
Oxidation Products ◽  
Chronic Alcohol ◽  
Chronic Alcohol Consumption ◽  
Radical Oxidation ◽  
Chronic Alcohol Intoxication

The research results of level glycemia, contents of free radical oxidation products (thiobarbiturate-reactive substances, oxidized-modified proteins) in blood plasma and heart of diabetes mellitus rats with chronic alcohol intoxication are presented. It is shown, that at presence of a diabetes mellitus the chronic alcohol consumption does not change blood plasma levels of the oxidized-modified proteins, thiobarbiturate-reactive substances and glucose. However the contents of thiobarbiturate-reactive substances and oxidizing modification of proteins products in animals heart is more considerably increased at combination of the diabetes with chronic alcohol consumption, in comparison with changes at the diabetes mellitus outside of alcoholization and with changes at the isolated chronic alcohol influence. Found out alcohol-induced free radical processes hyperactivation in heart at the diabetes is capable to render additional injuring influence.

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