scholarly journals Flow injection analysis of vancomycin

2018 ◽  
Vol 33 (2) ◽  
pp. 67
Author(s):  
Marta Maria Duarte Carvalho Vila ◽  
Acacia Adriana Salomao ◽  
Matthieu Tubino
Keyword(s):  
Quantitative Analysis ◽  
Detection Limit ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Copper Ions ◽  
Complete Agreement ◽  
Significance Level ◽  
Statistical Comparison ◽  
Spectrophotometric Methods ◽  
Quantitation Limit

A flow injection method for the quantitative analysis of vancomycin hydrochloride, C66H75Cl2N9O24.HCl (HVCM), based on the reaction with copper (II) ions, is presented. HVCM forms a lilac-blue complex with copper ions at pH≅4.5 in aqueous solutions, with maximum absorption at 555 nm. The detection limit was estimated to be about 8.5×10-5 mol L-1; the quantitation limit is about 2.5×10-4 mol L-1 and about 30 determinations can be performed in an hour. The accuracy of the method was tested through recovery procedures in presence of four different excipients, in the proportion 1:1 w/w. The results were compared with those obtained with the batch spectrophotometric and with the HPLC methods. Statistical comparison was done using the Student’s procedure. Complete agreement was found at a 0.95 significance level between the proposed flow injection and the batch spectrophotometric methods, which present similar precision (RSD: 2.1 % vs. 1.9%).

scholarly journals Flow injection analysis of vancomycin

2008 ◽  
Vol 33 (2) ◽  
pp. 67-72 ◽  
Author(s):  
M. M. D. C. Vila ◽  
A. A. Salomão ◽  
M. Tubino
Keyword(s):  
Quantitative Analysis ◽  
Detection Limit ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Copper Ions ◽  
Complete Agreement ◽  
Significance Level ◽  
Statistical Comparison ◽  
Spectrophotometric Methods ◽  
Quantitation Limit

A flow injection method for the quantitative analysis of vancomycin hydrochloride, C66H75Cl2N9O24.HCl (HVCM), based on the reaction with copper (II) ions, is presented. HVCM forms a lilac-blue complex with copper ions at pH≅4.5 in aqueous solutions, with maximum absorption at 555 nm. The detection limit was estimated to be about 8.5×10-5 mol L-1; the quantitation limit is about 2.5×10-4 mol L-1 and about 30 determinations can be performed in an hour. The accuracy of the method was tested through recovery procedures in presence of four different excipients, in the proportion 1:1 w/w. The results were compared with those obtained with the batch spectrophotometric and with the HPLC methods. Statistical comparison was done using the Student's procedure. Complete agreement was found at a 0.95 significance level between the proposed flow injection and the batch spectrophotometric methods, which present similar precision (RSD: 2.1 % vs. 1.9%).

Competitive heterogeneous enzyme immunoassay for theophylline by flow-injection analysis with electrochemical detection of p-aminophenol

1990 ◽  
Vol 36 (4) ◽  
pp. 662-665 ◽  
Author(s):  
E P Gil ◽  
H T Tang ◽  
H B Halsall ◽  
W R Heineman ◽  
A S Misiego
Keyword(s):  
Alkaline Phosphatase ◽  
Detection Limit ◽  
Electrochemical Detection ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
General Procedure ◽  
Amperometric Detection ◽  
Enzyme Label ◽  
Commercial Kit

Abstract A competitive enzyme-linked immunoabsorbent assay based on the flow-injection amperometric detection of p-aminophenol has been investigated with use of the materials and general procedure of a commercial kit for the determination of theophylline in human serum. The antibody is immobilized on glass beads, and the enzyme label is alkaline phosphatase (EC 3.1.3.1). The high currents generated during the electrochemical detection allowed a rapid (35 min) and simple determination of theophylline throughout its therapeutic range (10-20 mg/L) and also in the subtherapeutic range (detection limit of about 80 micrograms/L).

Rapid Quantitative Analysis of Multiple Explosive Compound Classes on a Single Instrument via Flow‐Injection Analysis Tandem Mass Spectrometry

2018 ◽  
Vol 64 (1) ◽  
pp. 223-230 ◽  
Author(s):  
Alla Ostrinskaya ◽  
Roderick R. Kunz ◽  
Michelle Clark ◽  
Richard P. Kingsborough ◽  
Ta‐Hsuan Ong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Tandem Mass Spectrometry ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Tandem Mass ◽  
Single Instrument

Nonimmunological Assay of Urinary Albumin Based on Laser-Induced Fluorescence

1992 ◽  
Vol 38 (10) ◽  
pp. 2089-2092 ◽  
Author(s):  
M A Kessler ◽  
M R Hubmann ◽  
B A Dremel ◽  
O S Wolfbeis
Keyword(s):  
Detection Limit ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Serum Proteins ◽  
Early Stage ◽  
Laser Induced Fluorescence ◽  
Urinary Albumin ◽  
Coefficients Of Variation ◽  
Flow Injection Analysis System ◽  
Analysis System

Abstract We describe the first nonimmunological assay of albumin in urine with a detection limit of 1 mg/L. The method is simple, rapid, and accurate. It is based on the probe Albumin Blue 670, which becomes highly fluorescent on binding to albumin. An inexpensive diode laser was used as the light source for measurement of laser-induced fluorescence. The assay was coupled to a flow-injection analysis system capable of running 20 samples per hour. The working range was 1-100 mg/L, which covered albumin concentrations found in nonpathological urine and in urine with slightly increased albumin. This range makes prediction of nephropathy possible at an early stage. Other serum proteins and hemoglobin do not interfere. The coefficients of variation were < 4% and < 7% within one day and from day to day, respectively. A correlation coefficient of 0.990 (n = 100) was obtained for comparison with the Behring nephelometric assay.

scholarly journals Determination of Thiocyanate by Kinetic Spectrophotometric Flow Injection Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Parinaz Elahian
Keyword(s):  
Detection Limit ◽  
Hydrochloric Acid ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Calibration Graph ◽  
Well Water ◽  
Janus Green ◽  
Rapid Flow ◽  
Kinetic Spectrophotometric

A simple and rapid flow injection spectrophotometric method has been developed for the determination of amount of thiocyanate in wastewaters and well water samples. It is based on the reaction of thiocyanate in hydrochloric acid with janus green and bromate. Reaction was monitored spectrophotometrically by measuring janus green absorbance atλmax=554 nm. The calibration graph was linear over the range 0.02–1.0 μg mL−1and the detection limit was 0.016μg mL−1(s/n=3). The throughput was 25 samples per hour.

scholarly journals Simultaneous Determination of Magnesium (Mg2+) and Ammonium (NH4+) by Flow Injection Analysis

2018 ◽  
Vol 19 (1) ◽  
pp. 1
Author(s):  
Mulik Kholifa ◽  
Tri Mulyono ◽  
Yeni Maulidah Muflihah
Keyword(s):  
Detection Limit ◽  
Simultaneous Determination ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Limit Of Detection ◽  
Detection Sensitivity ◽  
Magnesium Ions ◽  
Ammonium Ions ◽  
Percent Recovery

An automatic method for the simultaneous determination of magnesium and ammoniun by flow injection analysis is described. This analysis used two detectors, potentiometry and spectrophotometry, for the determination of magnesium and ammonium simultaneously. Potentiometric detectors are used to detect ammonium ions, whereas spectrophotometer detectors are used to detect the presence of magnesium ions. The performance of the FIA system includes linear range linearity, limit of detection, sensitivity, reproducibility and recovery test. Spectrophotometry FIA showed good performance with several criteria of linearity with regression value of 0.9918, the detection limit of 0.06 ppm, a sensitivity of 0.0115, repeatability of less than 2% and the percent recovery of 91,6%. While potentiometric FIA showed th performance with several criteria of linearity with regression value of 0.9896, the detection limit of 0.17 ppm, a sensitivity of 40.747 mV per decade of concentration, repeatability of less than 2% and the percent recovery of 89,7%. Keywords: Flow Injection Analysis, spectrophotometry, potensiometry, simultaneous

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