scholarly journals MORPHOLOGY AND HISTOLOGY OF THE EURASIAN LYNX (Lynx lynx) PLANUM NASALE

2021 ◽  
Vol 58 (4) ◽  
Author(s):  
Hasan Hüseyin Ari ◽  
Sema Uslu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microscopic Examination ◽  
Lynx Rufus ◽  
Glabrous Skin ◽  
Lynx Lynx ◽  
Eurasian Lynx ◽  
The Republic ◽  
Republic Of Turkey ◽  
Scanning Electron

Abstract: This study reveals the macroscopic and microscopic structures of the Eurasian lynx planum nasale using materials from three dead females obtained from the Sivas Forestry Branch of Agriculture and Forestry Ministry of the Republic of Turkey. To accomplish the purpose, planum nasale was investigated using macroscopic, histological, and scanning electron microscopy (SEM) techniques. The microscopic examination showed that the planum nasale consists of hairless, moist, glabrous skin and resembles a ship anchor with arm, palm, stock, and sickle parts. The planum nasale’s surface is formed by epidermal plates or epidermal ridges, which  were separated from each other by primary and secondary fissures showed in SEM and macroscopic figures. Based on the microscopic examination, the Mercel’s cells and nerve ends are located in the basal sheet of the planum nasale’s epidermal layers. In addition, the pores situated on the surface of the epidermal ridges and the dense connective bundles were settled in the dermal layers, based on the SEM examination.Key words: Eurasian lynx (Lynx rufus); morphology; nasal plane; planum nasaleMORFOLOGIJA IN HISTOLOGIJA SMRČKA EVRAZIJSKEGA RISA (Lynx lynx)Izvleček: V študiji so opisane makroskopske in mikroskopske strukture smrčka evrazijskega risa, ki je bila opravljena s proučevanjem tkiv treh mrtvih samic, ki so jih pridobili s pomočjo gozdarske podružnice Sivas Ministrstva za kmetijstvo in gozdarstvo Republike Turčije. Strukturo smrčka so raziskovali z uporabo makroskopskih, histoloških metod ter uporabe vrstičnega elektronskega mikroskopa (SEM). Mikroskopska preiskava je pokazala, da smrček sestavlja brezdlaka, vlažna, gola koža, ki po obliki spominja na ladijsko sidro. Površinski del smrčka tvorijo epidermalne plošče ali grebeni, ki jih ločujejo primarne in sekundarne razpoke, vidne na makroskopskih slikah in s pomočjo vrstične mikroskopije. Na histoloških preparatih so bile v bazalni plasti smrčka epidermisa opazne Merkelove celice in živčni končiči. S pomočjo metode SEM so v plasti epidermisa pokazali pore, ki se nahajajo na površini epidermalnih grebenov in snope togega fibrilarnega veziva, ki segajo v plast dermisa.Ključne besede: Evrazijski ris (Lynx rufus); morfologija; nosna ravnina; smrček

Sellaphora pelagonica (Bacillariophyceae), a new species from dystrophic ponds in the Republic of North Macedonia

Phytotaxa ◽  
2021 ◽  
Vol 496 (2) ◽  
pp. 121-133
Author(s):  
HRISTINA KOCHOSKA ◽  
DUŠICA ZAOVA ◽  
ANASTASIJA VIDESKA ◽  
DANIJELA MITIC-KOPANJA ◽  
HRISTINA NAUMOVSKA ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
The Republic ◽  
A New Species ◽  
Scanning Electron ◽  
Intermittent Ponds

Lowland intermittent ponds, although biologically important, are poorly studied water habitats. During the analyses of the material taken from lowland pond in Pelagonia Valley, and Vitachevo, Republic of North Macedonia, the diatom Sellaphora pelagonica sp. nov. was recorded and described based on light and scanning electron microscopy. The species is characterized by a strictly linear valve outline with parallel to slightly concave margins and broadly rounded ends, 15.0–40.0 µm long and 5.0–7.0 µm wide. These features, together with raphe structure and areolar shape are compared with several similar taxa currently ascribed to Sellaphora.

Revision of the family Carabodidae (Acari: Oribatida) XIII. Congocepheus camerunensis sp. nov. from Cameroon and Synkrotima zimbabwae gen. nov., sp. nov. from the Republic of Zimbabwe

2017 ◽  
Vol 22 (11) ◽  
pp. 1822 ◽  
Author(s):  
Nestor Fernandez ◽  
Pieter Daniel Theron ◽  
Sergio Leiva ◽  
Louwrens Tiedt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
The Family ◽  
The Republic ◽  
Scanning Electron ◽  
Shape And Size

Congocepheus camerunensis sp. nov. from Cameroon and Synkrotima zimbabwae gen. nov., sp. nov. from Zimbabwe are described using optical and Scanning Electron Microscopy (SEM). Congocepheus camerunensis is similar to C. heterotrichus, differentiated by: dissimilarity in shape and length of prodorsal, notogastral, epimeral, agenital, and adanal setae; placement, shape, and size of finger-like projection, bothridial ring and tutorium. Synkrotima zimbabwae gen. nov., sp. nov. is a distinctive species with a more or less flat elevated interlamellar process with longitudinal elevations and an anterior expansion; very complex ventral ornamentations with alternating depressed and elevated zones; genital neotrichy; absence of notogastral depression; the differences in shape and lengths of the prodorsal, notogastral, epimeral, genital, aggenital, adanal and anal setae in comparison to other species of the family Carabodidae. 

scholarly journals Isolation of Fonsecaea pedrosoi from thorns of Mimosa pudica, a probable natural source of chromoblastomycosis

2004 ◽  
Vol 46 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Claudio Guedes Salgado ◽  
Jorge Pereira da Silva ◽  
José Antônio Picanço Diniz ◽  
Moisés Batista da Silva ◽  
Patrícia Fagundes da Costa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Clinical Diagnosis ◽  
Microscopic Examination ◽  
Natural Source ◽  
Mimosa Pudica ◽  
Fonsecaea Pedrosoi ◽  
Source Of Infection ◽  
Direct Microscopic Examination ◽  
Scanning Electron

We report the isolation of Fonsecaea pedrosoi from thorns of the plant Mimosa pudica L. at the place of infection identified by one of our patients. Clinical diagnosis of chromoblastomycosis was established by direct microscopic examination and cultures from the patient's lesion. The same species was isolated from the patient and from the plant. Scanning electron microscopy of the surface of the thorns showed the characteristic conidial arrangement of F. pedrosoi. These data indicate that M. pudica could be a natural source of infection for the fungus F. pedrosoi.

scholarly journals The Role of Scanning Electron Microscopy in the Direct Diagnosis of Onychomycosis

Scanning ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Xueping Yue ◽  
Aiping Wang ◽  
Qing Li
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Clinical Practice ◽  
Microscopic Examination ◽  
Potassium Hydroxide ◽  
Fungal Culture ◽  
Test Results ◽  
Direct Microscopic Examination ◽  
Scanning Electron

Purpose. The purpose of this study was to evaluate the role of scanning electron microscopy (SEM) in the direct diagnosis of suspected onychomycosis with negative mycological test results.Methods. Outpatients diagnosed with suspected onychomycosis with negative mycological test results, including direct microscopic examination with 10% potassium hydroxide (KOH) and fungal culture, on 3 separate occasions were recruited. A small piece of infected nail was obtained for SEM examination.Results. Among the 48 suspected onychomycosis samples, SEM revealed that 18 (37.5%) were positive for fungal structures, including 10 (20.8%) cases of hyphae and 8 (16.7%) cases of yeast blastospores or budding.Conclusion. SEM represents an effective method to diagnose suspected onychomycosis when the traditional mycological methods were negative. Therefore, this technique could be used in clinical practice.

Microscopic Examination of Chitosan–Polyphosphate Beads with Entrapped Spores of the Biocontrol Agent,Streptomyces melanosporofaciensEF-76

2005 ◽  
Vol 11 (2) ◽  
pp. 154-165 ◽  
Author(s):  
Guy Jobin ◽  
Gilles Grondin ◽  
Geneviève Couture ◽  
Carole Beaulieu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microscopic Examination ◽  
Biocontrol Agent ◽  
Porous Surface ◽  
Macromolecular Complex ◽  
Complex Coacervation ◽  
Chitosan Beads ◽  
Streptomyces Melanosporofaciens ◽  
Scanning Electron

Spores of the biocontrol agent,Streptomyces melanosporofaciensEF-76, were entrapped by complex coacervation in beads composed of a macromolecular complex (MC) of chitosan and polyphosphate. A proportion of spores entrapped in beads survived the entrapment procedure as shown by treating spores from chitosan beads with a dye allowing the differentiation of live and dead cells. The spore-loaded chitosan beads could be digested by a chitosanase, suggesting that, once introduced in soil, the beads would be degraded to release the biocontrol agent. Spore-loaded beads were examined by optical and scanning electron microscopy because the release of the biological agent depends on the spore distribution in the chitosan beads. The microscopic examination revealed that the beads had a porous surface and contained a network of inner microfibrils. Spores were entrapped in both the chitosan microfibrils and the bead lacuna.

scholarly journals Microstructure and Degradation of Mortar Containing Waste Glass Aggregate as Evaluated by Various Microscopic Techniques

Materials ◽  
2020 ◽  
Vol 13 (9) ◽  
pp. 2186 ◽  
Author(s):  
Przemysław Czapik
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microscopic Examination ◽  
Energy Dispersive Spectroscopy ◽  
Test Procedure ◽  
Waste Glass ◽  
Alkali Silica Reaction ◽  
Glass Aggregate ◽  
Microscopic Techniques ◽  
Scanning Electron

The primary aim of this article is to focus on the alkali-silica reaction (ASR) in mortar specimens containing coloured waste glass used as an aggregate. Mortar expansion was measured using the ASTM C 1260 accelerated test procedure until the specimens disintegrated. Special attention was paid to the microscopic examination of the damaged mortar. Various methods were used for this purpose, including optical microscopy in reflected and transmitted light with one and two crossed polarizers. The specimens were also subjected to the scanning electron microscopy observations with energy dispersive spectroscopy (SEM-EDS). The data obtained from these techniques provided information on the mechanism of glass-containing mortar degradation due to ASR and also allowed the comparison of different microscopic techniques in terms of the information they can provide on ASR occurrence.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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