The use of carbon nanotubes to increase the sensitivity of the antibiotic determination with a piezoelectric immunosensor

2021 ◽  
Vol 87 (12) ◽  
pp. 11-16
Author(s):  
E. I. Shinko ◽  
O. V. Farafonova ◽  
T. N. Ermolaeva
Keyword(s):  
Carbon Nanotubes ◽  
Carbon Nanomaterials ◽  
Biological Fluids ◽  
Piezoelectric Sensor ◽  
Surface Concentration ◽  
Duration Of Treatment ◽  
Selective Determination ◽  
Piezoelectric Immunosensor ◽  
The Stability

Conditions for the preparation of carbon nanomaterials for embedding into the discerning layer of a piezoelectric immunosensor are described. The effect of the oxidation method, temperature, and the duration of treatment of nanomaterials with an oxidizing agent on the surface concentration of active functional groups is demonstrated. It is shown that the use of carboxylated carbon nanotubes (CNT) increases the efficiency of their binding to biomolecules and increases the stability of the discerning layer of a piezoelectric sensor when measurements are carried out in liquid media. Conditions for the determination of antibiotics using piezoelectric immunosensors modified with carbon nanomaterial were studied including the choice of immunoreagent concentrations and assessment of the selectivity of antibiotic determination. The CNT-based piezoelectric immunosensors providing rapid, highly sensitive, and selective determination of the analyte at the MRL level and below it in food products and biological fluids are proposed.

scholarly journals Derivative Spectrophotometric Analysis of 4-Quinolone Antibacterials in Formulations and Spiked Biological Fluids by Their Cu(II) Complexes

2001 ◽  
Vol 84 (2) ◽  
pp. 368-375 ◽  
Author(s):  
Mohammed Rizk ◽  
Fathalla Belal ◽  
Fawiza Ibrahim ◽  
Soad Ahmed ◽  
Zeinab A Sheribah
Keyword(s):  
Aqueous Medium ◽  
Linear Correlation ◽  
Analytical Procedure ◽  
Biological Fluids ◽  
Reference Method ◽  
Spectrophotometric Analysis ◽  
Third Order ◽  
Selective Determination ◽  
Average Percentage

Abstract A derivative UV-spectrophotometric analytical procedure was developed for determination of three 4-quinolone antibacterials: norfloxacin (NFX), ciprofloxacin (CFX), and sparfloxacin (SFX). The method depends on the complexation of Cu(II) with the studied compounds in aqueous medium. A third order, measurement was applied for their quantification. A linear correlation was established between the amplitude of the peak and concentration for all the studied drugs in the range of 15–80, 35–120, and 200–700 ng/mL, with minimum detectability (S/N = 2) of 1.0, 1.3, and 5.1 ng/mL for NFX, CFX, and SFX, respectively. The method was successfully applied for accurate, sensitive, and selective determination of the studied drugs in bulk and tablets formulation with average percentage recoveries of 99.22 ± 0.55 to 100.33 ± 1.60. The results obtained were favorably compared with those of the reference method. The method was also used to determine sparfloxacin in spiked human plasma and urine. The results obtained were satisfactory, accurate, and precise.

scholarly journals Nanomaterial-Based Carbon Paste Electrodes for Voltammetric Determination of Naproxen in Presence of Its Degradation Products

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Hassan A. M. Hendawy ◽  
Waheed M. Salem ◽  
Mahmmoud S. Abd-Elmonem ◽  
Elmorsy Khaled
Keyword(s):  
Carbon Nanotubes ◽  
Carbon Nanomaterials ◽  
Degradation Products ◽  
Phosphate Buffer Solution ◽  
Pharmaceutical Preparations ◽  
Matrix Composition ◽  
Human Blood Plasma ◽  
Carbon Paste ◽  
Carbon Paste Electrodes

The present work describes a novel, simple, and fast electroanalytical methodology for naproxen (NAP) determination in pharmaceutical formulations and biological fluids in the presence of its degradation products. Carbon paste electrodes (CPEs) modified with different carbon nanomaterials, namely, glassy carbon powder (GCE), multiwall carbon nanotubes (MWCNTs), single-walled carbon nanotubes (SWCNTs), graphene nanosheets (Gr), and graphene oxides (GO) were tested. Comprehensive studies were performed on the electrode matrix composition including the nature of the pasting liquids, pH, carbon nanomaterials, and mode of electrode modification. Two anodic oxidation peaks were recorded at 0.890 and 1.18 V in 1 × 10−1 mol·L−1 phosphate buffer solution at pH 6. Oxidation of naproxen (NAP) is an irreversible diffusion-controlled process. Calibration plots were rectilinear in the concentration ranging from 0.067 to 1.0 µg·mL−1 with correlation coefficient 0.9979. Photodegradation of NAP resulted in disappearance of the oxidation peak at 1.18 V, allowing simultaneous determination of NAP in presence of its degradation product. The achieved high sensitivity and selectivity suggest the application of the proposed protocol for naproxen determination in pharmaceutical preparations and human blood plasma.

scholarly journals Selective Determination of Dopamine in Presence of Ascorbic Acid Using Nafion Coated Methylene Blue Functionalized Graphite Electrode

2019 ◽  
Vol 31 (10) ◽  
pp. 2185-2190
Author(s):  
G. Sivasankari ◽  
K. Sivasankari ◽  
T. Nalini ◽  
P. Padmapriya ◽  
M. Nadhiya
Keyword(s):  
Methylene Blue ◽  
Ascorbic Acid ◽  
Modified Electrode ◽  
Electrode Surface ◽  
Graphite Electrode ◽  
Low Cost ◽  
Differential Pulse ◽  
Selective Determination ◽  
The Stability

An electrochemical sensor has been developed using methylene blue (MB) unctionalized graphite for the selective determination of dopamine in the presence of ascorbic acid. The immobilization of methylene blue on graphite was done by carbodiimide coupling method. The methylene blue functionalized graphite was characterized by SEM, FTIR spectroscopy and UV-visible spectroscopy. The immobilization of methylene blue functionalized graphite (MB-G) on the electrode surface was made by drop casting a small volume of MB-G dispersed methanol solution on the electrode surface. About 5 L of 0.5 % Nafion solution was also dropcasted for the stability and selectivity. The MB-G modified electrode was characterized by cyclic voltammetry. The modified electrode promotes the electrocatalytic oxidation of dopamine at a lower potential of 160 mV. The selective determination of dopamine in the presence of 100 times higher concentration of ascorbic acid was achieved by differential pulse voltammetry. The modified electrode offered a simple, selective and sensitive determination of low levels of dopamine. Also the oxidation over potential was reduced to an extent of 360 mV comparing with the bare graphite electrode. The proposed sensor has the advantage of easy fabrication, low cost, good sensitivity, reproducibility and stability.

scholarly journals Cost-Effective Potentiometric Platforms Modified with Multi-Walled Carbon Nanotubes (MWCNTs) and Based on Imprinted Receptors for Fluvoxamine Assessment

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 673
Author(s):  
Heba M. Hashem ◽  
Saad S. M. Hassan ◽  
Ayman H. Kamel ◽  
Abd El-Galil E. Amr ◽  
E. M. AbdelBary
Keyword(s):  
Carbon Nanotubes ◽  
Electrochemical Impedance ◽  
Biological Fluids ◽  
Pharmaceutical Preparations ◽  
Cost Effective ◽  
Hplc Method ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Screen Printed

A simple, efficient and reliable analytical method was developed and used for the determination of the fluvoxamine drug (FLV) in pharmaceutical preparations and biological fluids. The method is based on the cost-effective screen-printed platform for the potential transduction of the drug. Host-tailored molecular imprinting polymer (MIP) was integrated with the potentiometric platform as a recognition receptor, in which FLV, acrylamide (AAm), ethylene glycol dimethacrylate (EGDMA) and acetonitrile were used as a template, functional monomer, cross-linker, and solvent, respectively. MIP particles were dispersed in plasticized poly (vinyl chloride) (PVC) and the membrane was drop-casted on carbon screen-printed electrode. The MIP, in addition to non-imprinted polymers (NIP), was characterized and the binding experiment revealed high affinity and adsorption capacity of MIP towards FLV. The proposed sensor displayed near-Nernstian cationic slope of 55.0 ± 0.8 mV/decade (r2 = 0.999) with a low detection limit of 4.8 × 10−6 mol/L over a wide pH range (3.0–8.5). The electrochemical features of the proposed sensors including electrochemical impedance spectroscopy (EIS) and chronopotentiometry measurements (CP) in the presence of multi-walled carbon nanotubes (MWCNTs) as a solid contact transducer were also investigated. The applications of the proposed sensor for the determination of FLV in different dosage forms with recovery values (98.8%–101.9%) and (97.4%–101.1%), respectively compared with the reference HPLC method with acceptedFandt-student tests values at the 95% confidence level.

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