Effect of Ethanol Extract and Ethylacetate Fraction of Areca Seeds (Areca Catechu L.) on Changes in Worm Intensity in Mice (Mus Musculus) Given Per-Oral Infectious Trichuris Muris Eggs

BACKGROUND: Trichuris trichiura is a soil-transmitted parasitic worm commonly found in humid, tropical to subtropical areas, as well as poor sanitation area. These worms are cosmopolitan, especially in tropical and humid countries like Indonesia. This worm infection is more common in developing countries and more in children than adults due to poorer child self-hygiene. Worm disease is one of the common society diseases with 60% of children in Indonesia which are estimated to be affected. One species of T. trichiura worm that is often used in research on experimental animals is Trichuris muris. The administration of T. muris infective eggs can significantly increase the number of goblet cells in intestinal crypts of colon and cecum. Goblet cell hyperplasia will occur after exposure to high-dose T. muris (acute/200 infective eggs) in resistant mice. Various studies on the development of natural and traditional materials have been developing in the past few years to see the effects of betel nut on the number of goblet cells in the large intestine. AIM: The aim of this research is to determine the effect of ethanol extract and the ethyl acetate fraction of betel nut on the change in the number of goblet cells in male mice given orally T. muris infective eggs. METHODS: This study was an experimental study with a post-test only control group design in male mice (Mus musculus) which were given 200 infective eggs of T. muris. The study sample consisted of 70 mice divided into seven groups. Doses of the ethanol extract of betel nut are 100 mg/kg b.w. and 150 mg/kg b.w., and doses of ethyl acetate fraction are 100 mg/kg b.w. and 150 mg/kg b.w. The independent variable was ethanol extract and ethyl acetate fraction of betel nut. The dependent variable is the number of goblet cells. Mean differences in groups were tested by Mann–Whitney. RESULTS: Statistical analysis showed a significant difference of p < 0.05 in the colonic crypts and cecum. In Group K (+), there was no increase in the number of goblet cells (54.2000 ± 30.54864) compared to Group K (−) (79.0750 ± 11.79221) in the colon. There was an increase in goblet cells in the 150 mg/kg b.w. of ethanol extract group (64.1000 ± 29.50381), the 150 mg/kg b.w. of ethyl acetate fraction group (56.2250 ± 22.11035), and the 1 mg/20 g of albendazole group (68.3750 ± 11.30956) in the colon. Likewise, there was an increase in the number of goblet cells in the cecum. It can be concluded that 150 mg/kg b.w. of the ethanol extract of betel nut can effectively increase the number of goblet cells in the colon and cecum. CONCLUSION: Ethanol extract of betel nut 150 mg / kg of weight can effectively increase the number of goblet cells in the colon and caecum.

Download Full-text

UJI AKTIVITAS EKSTRAK ETANOL BIJI PINANG (Areca catechu L.) TERHADAP PENYEMBUHAN LUKA BAKAR PADA KULIT PUNGGUNG MENCIT PUTIH JANTAN (Mus musculus)

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i2.60 ◽

2017 ◽

Vol 2 (2) ◽

pp. 154

Author(s):

Fitri Handayani ◽

Reksi Sundu ◽

Henriko Nober Karapa

Keyword(s):

Antimicrobial Activity ◽

Wound Healing ◽

Treatment Group ◽

Mus Musculus ◽

Healing Process ◽

Ethanol Extract ◽

Phytochemical Analysis ◽

Burn Wound ◽

Burn Wound Healing ◽

Areca Catechu

Areca seeds contain flavonoid, tannin, and alkaloid which can help the healing process of burn wounds on the back skin of mice. Areca seeds are used for treatment because it has antimicrobial activity. Areca Seeds are one of Indonesian plants which the fruit in tranditional way is used as burn medicine. The objective of this study was to know theeffect of ethanol extract of areca seeds against burn wound healing on the back skin of mice. Fifteen mices were used in this experiment and were divided into 5 groups : vaseline flavum as a control negative, branded ointment as a control positive , ethanol extract of areca seeds in vaseline flavum with consentration 20%, 40 % and 60 %. Each group consisted of 3 mices. The back skin of mice was induced using hot iron size 1×1 cm for 2 seconds then given a treatment for 14 days. The percentage of healing of burns by calculating the surface area of wound. The results of its preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins and saponins. The percentage of burn wound healing of ethanol extract in concentration 20 % was 84,33 %, 40 % was 87,67%; and 60% was 89,67%. Data was analyzed using one-way Analysis of Variance (ANOVA) which showed that data has significant differences (P < 0,05) was 0,000. LSD test showed that each treatment group had siginicant differences with significant value < 0,05.

Download Full-text

Antidiabetic Potency of Jeruju (Acanthus ilicifolius L.) Ethanol Extract and Taurine on Histopathological Response of Mice Kidney (Mus musculus L.) Induced by Alloxan

Journal of Physics Conference Series ◽

10.1088/1742-6596/1751/1/012052 ◽

2021 ◽

Vol 1751 ◽

pp. 012052

Author(s):

E L Widiastuti ◽

B K Ardiansyah ◽

N Nurcahyani ◽

A Silvinia

Keyword(s):

Mus Musculus ◽

Ethanol Extract ◽

Histopathological Response ◽

Acanthus Ilicifolius

Download Full-text

THE EFFECTIVITY OF THE ETHANOL EXTRACT OF EGGPLANT (SOLANUM MELONGENA L.) PEELS AS ANTIMALARIAL TO MONOCYTE IN MICE (MUS MUSCULUS) INDUCED BY PLASMODIUM BERGHEI

Buletin Farmatera ◽

10.30596/bf.v4i3.3119 ◽

2019 ◽

Vol 4 (3) ◽

pp. 102

Author(s):

Ari Widiyantoro

Keyword(s):

Mus Musculus ◽

Plasmodium Berghei ◽

Solanum Melongena ◽

Ethanol Extract

Download Full-text

Efek Ekstrak Biji Labu Kuning Terhadap Glukosa, Kolesteroldan Gambaran Histopatologi Pankreas Tikus Hiperkolesterolemia-Diabetes

Talenta Conference Series Tropical Medicine (TM) ◽

10.32734/tm.v1i3.280 ◽

2018 ◽

Vol 1 (3) ◽

pp. 144-151

Author(s):

Joni Tandi ◽

Rahmawati Rahmawati ◽

Rini Isminarti ◽

Jerry Lapangoyu

Keyword(s):

Ethanol Extract ◽

Pancreatic Tissue ◽

Blood Cholesterol ◽

Maximum Effect ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

Cell Degeneration ◽

Pumpkin Seed ◽

Per Oral

Hiperkolesterolemia adalah peningkatan kadar kolesterol di dalam darah melebihi batas yang diperlukan oleh tubuh. Penelitan ini bertujuan menguji kandungan senyawa fitokimia ekstrak etanol biji labu kuning, efek pemberian ekstrak etanol biji labu kuning dan perbedaan efek ekstrak dengan dosis bertingkat terhadap penurunan degenerasi sel beta pankreas tikus putih jantan hiperkolesterolemia diabetes. Penelitian eksperimen laboratorium ini menggunakan hewan uji sebanyak 30 ekor tikus dibagi dalam enam kelompok perlakuan. Kelompok 1 (kontrol normal) diberikan Na-CMC 0,5% dan kelompok 2 (kontrol negatif) diberi pakan tinggi kolesterol, suspensi Streptozotocin 35 mg/kgBB dan Na-CMC 0,5% b/v; kelompok 3 diberi metformin 9 mg/kgBB per oral, pakan tinggi kolesterol dan suspensi streptozotocin 35 mg/kgBB; kelompok 4, 5 dan 6 masing-masing diberikan dosis 270, 360, dan 450 mg/kgBB per oral, pakan tinggi kolesterol dan suspensi streptozotocin 35 mg/kgBB. Gambaran tingkat kerusakan histopatologi pankreas diamati dengan pewarnaan HE menggunakan mikroskop Olympus BX-51 perbesaran 200x. Hasil penelitian menunjukkan: Terdapat senyawa alkaloid, flavonoid, polifenol, saponin,dan tannin pada ekstrak etanol biji labu kuning; ekstrak etanol biji labu kuning dosis 360 dan 450 mg/kgBB efektif menurunkan degenerasi jaringan pankreas tikus hiperkolesterolemia diabetes dan pemberian ekstrak etanol biji labu kuning dosis 270 mg/kgBB tidak memberikan efek maksimal jika dibandingkan dengan dosis 360 mg/kgBB dan dosis 450 mg/kgBB terhadap regenerasi sel organ pankreas tikus putih jantan hiperkolesterolemia diabetes. Hypercholesterolemia is an abnormal increase in blood cholesterol levels. This study aimed to identify the phytochemical content of pumpkin seed ethanol extract, the effect of pumpkin seed ethanol extract and the difference in the effect of various doses of extract in decreasing pancreatic beta cell degeneration in male diabetic hypercholesterolemia rat. The experimental research in laboratory used 30 rats as test animals, divided into six group. Group 1 (normal control administered with 0.5% Na CMC and group 2 (negative control) administered with high cholesterol diet, Streptozotocin 35 mg/kgBWand 0.5% Na-CMC b/v; Group 3 orally administered with metformin 9 mg/kgBW, high cholesterol diet, streptozotocin 35 mg/kgBW; group 4,5 and 6 orally administered with extract at doses of 270, 360, dan 450 mg/kgBB, high cholesterol diet, streptozotocin 35 mg/kgBW. Histopathology examination to determine pancreas damage was observed by HE staining using microscop Olympus BX-51 200x. The results showed the presence of alkaloids, flavonoids, poliphenol, saponins,andtanninsin phumpkin seeds ethanol extract; Ethanol extract of phumpkin seeds at doses of 360 dan 450 mg/kgBW effectively decreased degeneration of pancreatic tissue of diabetic hypercholesterolemia rat and ethanol extract of phumpkin seeds at the dose of 270 mg/kgBW did not show maximum effect as compared to the doses of 360 and 450 mg/kg BE on cell regeneration of pancreatic tissue of diabetic hypercholesterolemia rat

Download Full-text