The Potency of Kaffir Lime Leaf (Citrus Hystrix) Ethanol Extract Against Lead Acetate Toxicity on Spermatozoa Quality of Mice (Mus musculus)

2021 ◽  
Keyword(s):  
Mus Musculus ◽  
Lead Acetate ◽  
Ethanol Extract

scholarly journals Pengaruh Ekstrak Etanol dan Spinasterol Daun Senggugu (Clerodendron Serratum L.) terhadap Kualitas Sperma Mencit (Mus Musculus L.)

2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Desak Made Malini
Keyword(s):  
Body Weight ◽  
Mus Musculus ◽  
Sperm Quality ◽  
Ethanol Extract ◽  
Dmso Solution ◽  
Epididymal Sperm ◽  
The Public ◽  
Sperm Suspension ◽  
The Family

AbstrakTumbuhan senggugu (C. serratum L.) termasuk famili verbenaceae, merupakan salah satu tumbuhan yang cukup dikenal dimasyarakat sebagai tanaman obat. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun senggugu (C. serratum L.) terhadap kualitas sperma mencit (Mus musculus). Penelitian ini merupakan penelitian eksperimental yang menggunakan rancangan percobaan dengan pola Rancangan Acak Lengkap (RAL). Penelitian ini terdiri atas dua kelompok kontrol dan lima kelompok perlakuan dengan lama perlakuan 9 dan 18 hari. Setiap kelompok menggunakan 6 ekor mencit, sehingga jumlah mencit keseluruhan adalah 42 ekor. Parameter yang diamati adalah jumlah, motilitas, abnormalitas dan viabilitas sperma mencit. Data yang diperoleh dianalisis dengan Anava dan dilanjutkan dengan uji Duncan. Hasil penelitian ini menunjukkan bahwa dilihat dari hasil daun senggugu (C. serratum L.) dapat menurunkan kualitas mencit.Kata kunci : senggugu, mencit dan kulaitas sperma.AbstractPlant senggugu (C.serratum L.) belongs to the family Verbenaceae, which has been recogniced by the public as a medicinal plant. One of them is that it can be used as a drug antifertility in men. The purpose of this study is to find out the effect of ethanol extract and spinasterol of Senggugu ©.Serratum L.) leaves to the sperm quality of mouse (M. musculus) The study is conducted using complete random with 7X2 factor repeated 6 times. The treatment given to the ethanol extract is 250 mg/kg body weight, 500 mg/kg body weight and 1000 mg/kg body weight, as with spinasterol the amount is 26 mg/kg body weight and 52 mg/kg body weight, and as its controlling agent distilled water and DMSO solution is used. The treatment is given orally once a day within 9and 18 days as much as 0,5 ml. Cauda epididymal sperm suspension was used to measure the quality of the sperm of mice. Data were analyzed by ANOVA followed by Duncan test. Based on observation and statistical analysis it has been found that theethanol and spinasterol extract treatment of senggugu leaves using a diffrent dosage has shown a significant effects to the parameters being studied namely increased abnormality of sperm and decreased in number of sperm, percentage of motolityand viability sperm.Keyword : Clerodendron serattum, senggugu, Mus musculus, Spermatozoa, Ethanol extract, Spinasterol

scholarly journals Effects of Red Fruit (Pandanus conoideus Lam) Oil on Malondialdehyde Level and Spermatozoa Quality in Mice (Mus musculus) Exposed to Monosodium Glutamate

2018 ◽  
Vol 54 (2) ◽  
pp. 84 ◽  
Author(s):  
Widayati Agustina ◽  
Widjiati Widjiati ◽  
Alfiah Hayati
Keyword(s):  
Mus Musculus ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Distilled Water ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Post Test ◽  
Fruit Oil

This study aimed to determine the effects of red fruit (Pandanus conoideus Lam) oil on MDA levels and spermatozoa quality in mice (Mus musculus) exposed to MSG. The quality includes motility, viability, concentration, and morphology of spermatozoa. This experimental study used randomized post-test only control group design. The subjects of this study were 25 mice (Mus musculus), divided into 5 groups (5 mice per group). K- group received distilled water for 35 days. K+ group received 4 mg/g BW MSG for 21 days. P1, P2, and P3 treatment groups received 4 mg/g BW MSG for 21 days and 0.02; 0.04; 0.08 ml/g BW red fruit oil, respectively, from day 22 to 35. The results showed that mean spermatozoa morphology in K-, K+, P1, P2, P3 groups were as follows: 0.86; 0.56; 0.67; 0.61; and 0.87 (%). The spermatozoa concentrations were sequentially as follows: 21; 10; 15; 32,8,19 (107 cells/ml). The spermatozoa's vitalities were as follows: 0,64; 0,14; 0,24; P2: 0.36; 0.68 (%). MDA levels were respectively: 0.29; 0.60; 0.35; 0.23; and 0.19 (nm). As a conclusion, testicular MDA levels in mice exposed to MSG and given with red fruit oil were lower than those in mice exposed to MSG without receiving red fruit oil. The quality of spermatozoa in mice exposed to MSG and receiving red fruit oil was higher than that of mice exposed to MSG without being given with red fruit oil.

scholarly journals PENGARUH AIR REBUSAN AKAR ARU (Caesalpinia bonduc) TERHADAP KUALITAS SPERMA EPIDIDIMIS MENCIT (Mus musculus) : DASAR PENGEMBANGAN OBAT K0NTRASEPSI TRADISIONAL BAGI LAKI-LAKI

2013 ◽  
Vol 13 (2) ◽  
Author(s):  
D. Setiadi Dan S. Bachri
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Mus Musculus ◽  
Treated Group ◽  
Sperm Maturation ◽  
Motile Sperm ◽  
Abnormal Sperm ◽  
Sperm Abnormality ◽  
Boiled Water

AbstrakIndonesia memiliki banyak tumbuhan berpotensi obat salah satunya bisa dijadikan sebagai obatkontrasepsi tradisonal yang biasa digunakan untuk menjarangkan anak atau sterilisasi sepertirebusan akar Caesalpinia bonduc (Aru). Tujuan penelitian untuk mengetahui pengaruh obatkontrasepsi tradisional air rebusan akar aru terhadap kualitas pematangan sperma epididimis mencit(Mus musculus). Mencit dipilih secara acak untuk mewakili 4 kelompok dosis yaitu : 0,0 (K0), 16,0(K1) 24,0 (K2) dan 32,0 (K3) μl/gram berat badan/hari. Setiap kelompok perlakuan dilakukandengan 5 kali replikasi. Perlakuan diberikan melalui oral dengan menggunakan jarum gavage selama10 hari berturut-turut. Pembedahan dilakukan pada hari pertama setelah perlakuan selesai.Pengamatan kualitas sperma epididimis dengan perhitungan produksi sperma, % sperm motil, %sperma hidup, % sperma abnormal,dan rangking keaktifan sperma. Untuk mengetahui ada atautidaknya perbedaan yang bermakna antar perlakuan dalam kelompok dilakukan uji Anava satu arah,bila terdapat perbedaan bermakna dilanjutkan uji BNJ untuk membandingkan angka rata-rata antarkelompok perlakuan. Pemberian air rebusan akar Caesalpinea bonduc pada mencit menunjukanpengaruh perbedaan secara signifikan pada jumlah (%) sperma abnormal antara dosis 0,0 μl dengan24,0 dan 32,0 μl serta antara 16,0 μl dengan 24,0 dan 32,0 μl; Jumlah (%) sperma hidup terdapatperbedaan antara dosis 0,0 μl dengan 16,0 , 24,0 dan 32,0 μl; Jumlah (%) sperma motil terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl; Rangking keaktifan sperma terdapatperbedaan antara dosis 0,0 μl dengan 24,0 dan 32,0 μl. Pemberian rebusan akar aru berpengaruhsecara signifikan terhadap peningkatan persen sperma abnormal, dan penuruan persen sperma hidup,motil dan keaktifan.AbstractIndonesia has many species of plants that have potent of medicine, one of them cold be as antraditional contraception medicine ussually used to limit child or sterilizatiom such as root boiledwater of Caesalpinia bonduc. The aims of this study is to know the effect of root boiled water ofCaesalpinia bonduc as traditional contraception medicine on quality of sperm maturation ofepididymis of mice (Mus musculus). Mice were choosed radomly and doses gouped: 0,0 (K0 ), 16,0(K1) 24,0 (K2) and 32,0 (K3) μl/gram body weight/day. Each of group was replicated 5 times.Treatment were given by oral with using gavage needle for ten days. Surgery was carried out on firstday after completing treatments. Examination of epidymis sperm by counting number of spermproduction, percentage of motil, live, abnormal sperm, and rank of sperm motility. In order to knowthe deferences between control and treated group, was used one way anava analysis and analysedvalue for comparing between teratment group. The treatments of root boiled water of Caesalpiniabonduc have effect significantly on percentage of abnormal sperm between dose of 0,0 μl with 24,0and 32,0 μl, also between 16,0 μl with 24,0 and 32,0 μl. Percentage of live sperem is different betweendose of 0,0 μl with 16,0 , 24,0 and 32,0 μl. Percentage of motile sperm is defferent significantlybetween dose of 0,0 μl with 24,0 and 32,0 μl. Meanwhile percentage of motile rank has differencebetween dose of 0,0 μl with 24,0 and 32,0 μl. The treatment of root boiled water of Caesalpiniabonduc has effect signifiantly on increasing percentange of sperm abnormality, decreasingpercentage of life sperm, motile and rank of motile of mice sperm.

Improving the quality of perovskite based on lead acetate for efficient solar cell

2019 ◽  
Vol 254 ◽  
pp. 85-91
Author(s):  
Qiaoli Niu ◽  
Hao Lv ◽  
Xiaomeng Duan ◽  
Mao Jiang ◽  
Yewei Zhang ◽  
...  
Keyword(s):  
Solar Cell ◽  
Lead Acetate ◽  
Efficient Solar Cell

scholarly journals Effect of Ethanol Extract and Ethyl Acetate Fraction of Betel Nut (Areca catechu L.) in Colonic Goblet Cells of Mice (Mus musculus) Given Orally Infective Egg of Trichuris muris

2020 ◽  
Vol 8 (B) ◽  
pp. 637-642
Author(s):  
Endy Juli Anto ◽  
Aznan Lelo ◽  
Syafruddin Ilyas ◽  
Marline Nainggolan
Keyword(s):  
Ethyl Acetate ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Goblet Cells ◽  
High Dose ◽  
Betel Nut ◽  
Male Mice ◽  
Trichuris Muris ◽  
Significant Difference

BACKGROUND: Trichuris trichiura is a soil-transmitted parasitic worm commonly found in humid, tropical to subtropical areas, as well as poor sanitation area. These worms are cosmopolitan, especially in tropical and humid countries like Indonesia. This worm infection is more common in developing countries and more in children than adults due to poorer child self-hygiene. Worm disease is one of the common society diseases with 60% of children in Indonesia which are estimated to be affected. One species of T. trichiura worm that is often used in research on experimental animals is Trichuris muris. The administration of T. muris infective eggs can significantly increase the number of goblet cells in intestinal crypts of colon and cecum. Goblet cell hyperplasia will occur after exposure to high-dose T. muris (acute/200 infective eggs) in resistant mice. Various studies on the development of natural and traditional materials have been developing in the past few years to see the effects of betel nut on the number of goblet cells in the large intestine. AIM: The aim of this research is to determine the effect of ethanol extract and the ethyl acetate fraction of betel nut on the change in the number of goblet cells in male mice given orally T. muris infective eggs. METHODS: This study was an experimental study with a post-test only control group design in male mice (Mus musculus) which were given 200 infective eggs of T. muris. The study sample consisted of 70 mice divided into seven groups. Doses of the ethanol extract of betel nut are 100 mg/kg b.w. and 150 mg/kg b.w., and doses of ethyl acetate fraction are 100 mg/kg b.w. and 150 mg/kg b.w. The independent variable was ethanol extract and ethyl acetate fraction of betel nut. The dependent variable is the number of goblet cells. Mean differences in groups were tested by Mann–Whitney. RESULTS: Statistical analysis showed a significant difference of p < 0.05 in the colonic crypts and cecum. In Group K (+), there was no increase in the number of goblet cells (54.2000 ± 30.54864) compared to Group K (−) (79.0750 ± 11.79221) in the colon. There was an increase in goblet cells in the 150 mg/kg b.w. of ethanol extract group (64.1000 ± 29.50381), the 150 mg/kg b.w. of ethyl acetate fraction group (56.2250 ± 22.11035), and the 1 mg/20 g of albendazole group (68.3750 ± 11.30956) in the colon. Likewise, there was an increase in the number of goblet cells in the cecum. It can be concluded that 150 mg/kg b.w. of the ethanol extract of betel nut can effectively increase the number of goblet cells in the colon and cecum. CONCLUSION: Ethanol extract of betel nut 150 mg / kg of weight can effectively increase the number of goblet cells in the colon and caecum.

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