scholarly journals Efek Ekstrak Biji Labu Kuning Terhadap Glukosa, Kolesteroldan Gambaran Histopatologi Pankreas Tikus Hiperkolesterolemia-Diabetes

2018 ◽  
Vol 1 (3) ◽  
pp. 144-151
Author(s):  
Joni Tandi ◽  
Rahmawati Rahmawati ◽  
Rini Isminarti ◽  
Jerry Lapangoyu
Keyword(s):  
Ethanol Extract ◽  
Pancreatic Tissue ◽  
Blood Cholesterol ◽  
Maximum Effect ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cell Degeneration ◽  
Pumpkin Seed ◽  
Per Oral

Hiperkolesterolemia adalah peningkatan kadar kolesterol di dalam darah melebihi batas yang diperlukan oleh tubuh. Penelitan ini bertujuan menguji kandungan senyawa fitokimia ekstrak etanol biji labu kuning, efek pemberian ekstrak etanol biji labu kuning dan perbedaan efek ekstrak dengan dosis bertingkat terhadap penurunan degenerasi sel beta pankreas tikus putih jantan hiperkolesterolemia diabetes. Penelitian eksperimen laboratorium ini menggunakan hewan uji sebanyak 30 ekor tikus dibagi dalam enam kelompok perlakuan. Kelompok 1 (kontrol normal) diberikan Na-CMC 0,5% dan kelompok 2 (kontrol negatif) diberi pakan tinggi kolesterol, suspensi Streptozotocin 35 mg/kgBB dan Na-CMC 0,5% b/v; kelompok 3 diberi metformin 9 mg/kgBB per oral, pakan tinggi kolesterol dan suspensi streptozotocin 35 mg/kgBB; kelompok 4, 5 dan 6 masing-masing diberikan dosis 270, 360, dan 450 mg/kgBB per oral, pakan tinggi kolesterol dan suspensi streptozotocin 35 mg/kgBB. Gambaran tingkat kerusakan histopatologi pankreas diamati dengan pewarnaan HE menggunakan mikroskop Olympus BX-51 perbesaran 200x. Hasil penelitian menunjukkan: Terdapat senyawa alkaloid, flavonoid, polifenol, saponin,dan tannin pada ekstrak etanol biji labu kuning; ekstrak etanol biji labu kuning dosis 360 dan 450 mg/kgBB efektif menurunkan degenerasi jaringan pankreas tikus hiperkolesterolemia diabetes dan pemberian ekstrak etanol biji labu kuning dosis 270 mg/kgBB tidak memberikan efek maksimal jika dibandingkan dengan dosis 360 mg/kgBB dan dosis 450 mg/kgBB terhadap regenerasi sel organ pankreas tikus putih jantan hiperkolesterolemia diabetes.   Hypercholesterolemia is an abnormal increase in blood cholesterol levels.  This study aimed to identify the phytochemical content of pumpkin seed ethanol extract, the effect of pumpkin seed ethanol extract and the difference in the effect of various doses of extract in decreasing pancreatic beta cell degeneration in male diabetic hypercholesterolemia rat. The experimental research in laboratory used 30 rats as test animals, divided into six group. Group 1 (normal control administered with 0.5% Na CMC and group 2 (negative control) administered with high cholesterol diet, Streptozotocin 35 mg/kgBWand 0.5% Na-CMC b/v; Group 3 orally administered with metformin 9 mg/kgBW, high cholesterol diet,  streptozotocin 35 mg/kgBW; group 4,5 and 6 orally administered with extract at doses of 270, 360, dan 450 mg/kgBB, high cholesterol diet,  streptozotocin 35 mg/kgBW. Histopathology examination to determine pancreas damage was observed by HE staining using microscop Olympus BX-51 200x. The results showed the presence of alkaloids, flavonoids, poliphenol, saponins,andtanninsin phumpkin seeds ethanol extract; Ethanol extract of phumpkin seeds at doses of 360 dan 450 mg/kgBW effectively decreased degeneration of pancreatic tissue of diabetic hypercholesterolemia rat and ethanol extract of phumpkin seeds at the dose of 270 mg/kgBW did not show maximum effect as compared to the doses of 360 and 450 mg/kg BE on cell regeneration of pancreatic tissue of diabetic hypercholesterolemia rat

scholarly journals PEMBERIAN EKSTRAK ETANOL BUAH BUNCIS (Phaseolus vulgaris L.) UNTUK MENURUNKAN KOLESTEROL TOTAL, Low Density Lipoprotein (LDL) DAN MENINGKATKAN High Density Lipoprotein (HDL) PADA TIKUS WISTAR DIET TINGGI LEMAK

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Sri Wahjuni ◽  
Ni Luh Rustini ◽  
Putu Yuliantari
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Density Lipoprotein ◽  
Ethanol Extract ◽  
Hdl Cholesterol ◽  
Blood Cholesterol ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cholesterol Levels

Excessive fat consumption can increase blood cholesterol level. Phytosterol composition in the plant can decrease blood cholesterol level. One of the plants that contain phytosterol is beans. This study was to prove the effect of antihypercholesterol the ethanol extract of the beans (Phaseolus vulgaris L.) with variety of doses of 50 mg/Kg BW; 100 mg/Kg BW and 150 mg/Kg BW in male Wistar rats with high fat level condition. Subjects of this study were 24 Wistar rats divided into 6 groups randomly with posttest control group study design. First group as negative control, second group as positive control, and the third (high cholesterol diet and ethanol extract in dose of 50 mg/kg,bw), fourth ( high cholesterol diet and ethanol extractin dose of 100 mg/kg,bw), fifth ( high cholesterol diet and ethanol extract in dose of 150 mg/kg,bw),  sixth (high cholesterol diet and simvastatin drug). The data was analyzed with ANOVA Tukey test. The result of this study shows total cholesterol levels decrease 23,88%; 30,14%; 35,82%. Cholesterol LDL levels decrease 38,09%; 52,38%; 61,35%. Cholesterol HDL levels increase 21,86%; 56,56%; 63,84%. Dose variant on this study giving the best result to decrease total cholesterol levels and LDL was 150 mg/Kg BW. Furthermore, 100 mg/Kg BW dose gave the best result to increase HDL cholesterol level. Based on the results it can be suggested that beans ethanol extract is able to decrease total cholesterol level, LDL and increase HDL cholesterol levels.  

Soybean germ oil reduces blood cholesterol by inhibiting cholesterol absorption and enhancing bile acid excretion

2019 ◽  
Vol 10 (4) ◽  
pp. 1836-1845 ◽  
Author(s):  
Hanyue Zhu ◽  
Jingnan Chen ◽  
Zouyan He ◽  
Wangjun Hao ◽  
Jianhui Liu ◽  
...  
Keyword(s):  
Bile Acid ◽  
Cholesterol Absorption ◽  
Blood Cholesterol ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
Acid Excretion ◽  
High Cholesterol ◽  
Bile Acid Excretion

Soybean germ oil is beneficial in management of hypercholesterolemia in hamsters fed a high cholesterol diet.

scholarly journals Analisis ketersediaan kolesterol total, trigliserid, glukosa dan aktivitas enzim Alanine aminotransferase (ALT) dan Aspartate Aminotransferase (AST) pada ayam Broiler kolesterol tinggi pasca pemberian bawang putih (Allium sativum Iinn)

2018 ◽  
Vol 29 (2) ◽  
pp. 110
Author(s):  
Agustina Dwi Wijayanti ◽  
Rani Puspita Sari ◽  
Riyanto Riyanto
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Allium Sativum ◽  
Blood Cholesterol ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Total Blood ◽  
Total Blood Cholesterol

This study was to find out the effect of ethanolic garlic extrct of total blood cholesterol, trigliserid, glucone and the activities of alanine aminotransferase (ALT) dan Aspartate Aminotransferase (AST) of broiler fed with high cholesterol diet.

scholarly journals Early Effects of High Cholesterol Diet on the Kidney of an Animal Model

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Zenab B. Hamad Mohamed ◽  
Hamad Abdulsalam Hamad Alfarisi ◽  
Nor Zamzila Abdullah ◽  
Norra Harun ◽  
Naznin Muhammad ◽  
...  
Keyword(s):  
Animal Model ◽  
Serum Creatinine ◽  
Histological Examination ◽  
Blood Cholesterol ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Early Effect ◽  
The Impact

Introduction: Previous studies have proven the existence of a complex association between progressive kidney damage and hypercholesterolemia. Most studies focused on the impact of chronic high blood cholesterol levels on the kidney. Information on the early effect of hypercholesterolemia on the kidney is still lacking. The aim of this study was therefore to determine early effect of high cholesterol diet on the kidney in an animal model. Materials and method: Ten female Sprague-Dawley rats were divided into two groups. The control group was fed with commercial rat pellet while the high cholesterol diet (HCD) group was fed with 12% cholesterol diet and 0.3% cholic acid. Biochemical analysis (lipid profile and renal function) was performed at 48 hours, 7 days and 6 weeks of the experiment. The animals were sacrificed at 6 weeks and the kidneys were harvested for histological examination. Results: The HCD group rats had significantly higher levels of serum total cholesterol (at 7 days and 6 weeks). The HDL-cholesterol and triglyceride levels were however lower at 6 weeks. The mean serum creatinine level of the HCD group were increased after 48 hours and 7 days compared to the control group. Histological examination of the kidney tissue of the HCD group at 6 weeks revealed segmental mesangial hypercellularity and mesangial matrix expansion of the glomeruli. Conclusion: The 12% cholesterol diet induced dyslipidaemia in the animal model. It resulted in acute kidney injury based on the serum creatinine at 48 hours and 7 days. Kidney tissues examined at 6 weeks revealed changes confined to mesangial cells of the renal glomeruli.

scholarly journals Sericin ameliorated dysmorphic mitochondria in high-cholesterol diet/streptozotocin rat by antioxidative property

2016 ◽  
Vol 242 (4) ◽  
pp. 411-421 ◽  
Author(s):  
Sumate Ampawong ◽  
Duangnate Isarangkul ◽  
Pornanong Aramwit
Keyword(s):  
Endoplasmic Reticulum ◽  
Exocrine Pancreas ◽  
Protective Role ◽  
Blood Cholesterol ◽  
High Cholesterol Diet ◽  
Zymogen Granule ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Electron Microscopic ◽  
Antioxidative Property

Sericin has been implicated in lower cholesterolemic effect due to its properties with several mechanisms. Mitochondria are one of the most important targets to be affected in high blood cholesterol and glucose conditions. The protective role of sericin on mitochondria remains doubtful. To examine this role, electron microscopic, histopathologic, immunohistochemical, and biochemical studies were performed in a high-cholesterol diet/streptozotocin rat model. The results demonstrated that sericin reduced blood cholesterol without hypoglycemic effect. Sericin alleviated dysmorphic mitochondria in heart and liver but not in kidney and also decreased peculiar endoplasmic reticulum in the exocrine pancreas. In addition, sericin decreased hepatic steatosis and preserved zymogen granule referable to the decline of reactive oxygen species production in hepatic mitochondrial extraction and down-regulation of malondialdehyde expression in the liver and exocrine pancreas however irrelevant to lipase activity. This study suggests that sericin has antioxidative property to reduce blood cholesterol by means of diminishing fat deposit in hepatocyte and improves mitochondria and endoplasmic reticulum integrities. [Box: see text]

scholarly journals Metformin Ameliorates Testicular Function and Spermatogenesis in Male Mice with High-Fat and High-Cholesterol Diet-Induced Obesity

Nutrients ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1932
Author(s):  
Chin-Yu Liu ◽  
Ting-Chia Chang ◽  
Shyh-Hsiang Lin ◽  
Sheng-Tang Wu ◽  
Tai-Lung Cha ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Semen Quality ◽  
Blood Cholesterol ◽  
High Cholesterol Diet ◽  
Metformin Treatment ◽  
Cholesterol Diet ◽  
Male Mice ◽  
High Cholesterol ◽  
High Fat ◽  
Diet Induced Obesity

The aim of this study was to investigate the effects of metformin supplementation on metabolic dysfunction, testicular antioxidant capacity, apoptosis, inflammation and spermatogenesis in male mice with high-fat and high-cholesterol diet-induced obesity. Forty male C57BL/6 mice were fed a normal diet (NC group, n = 10) or a high-fat and high-cholesterol diet (HFC group, n = 30) for 24 weeks, and mice randomly chosen from the HFC group were later treated with metformin for the final 8 weeks of HFC feeding (HFC + Met group, n = 15). Compared with the HFC group, the obese mice supplemented with metformin exhibited improved blood cholesterol, glucose and insulin resistance. The HFC group diminishes in the sperm motility and normal sperm morphology, while the poorer maturity of testicular spermatogenesis was improved by metformin treatment. The HFC group exhibited a higher estradiol level and a lower 17β-HSD protein expression. Further analyses showed that metformin treatment increased the activities of superoxide dismutase, catalase and glutathione peroxidase and reduced lipid peroxidation. Nevertheless, both the HFC and HFC + Met groups exhibited increased expressions of apoptosis and inflammation proteins in the testis. Metformin treatment ameliorated obesity-induced poor testicular spermatogenesis and semen quality through increasing the testosterone level and antioxidant capacity.

scholarly journals Potential of Lactobacillus casei Shirota Strain Probiotic Toward Total Cholesterol Levels and Sod Activity in Rat with High Cholesterol Diet

Molekul ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 153
Author(s):  
Kiswanti Surya Utami ◽  
Aulanni'am Aulanni’am ◽  
Chanif Mahdi
Keyword(s):  
Total Cholesterol ◽  
Lactobacillus Casei ◽  
Positive Control ◽  
Male Rats ◽  
Blood Cholesterol ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Sod Activity ◽  
Lactobacillus Casei Shirota

Probiotic of Lactobacillus casei Shirota strain is lactic acid bacteria that have benefits to enhance immunity system, as an antioxidant and has the ability to decrease the cholesterol level. The aim of this study was to determine the potential of Lactobacillus casei Shirota strain probiotic toward total cholesterol decreasing and SOD activity increasing in rats’ aorta with a high-cholesterol diet. This study used 25 male rats of Rattus novergicus Wistar strain that will be divided into 5 groups. The 1st group as the negative control, and the 2nd group as positive control. The positive control group was induced by high-cholesterol diet contained quail egg yolk, pure cholesterol, cholic acid and waste oil that were given for 3 weeks. The 3rd, 4th and 5th group were treated by probiotic Lactobacillus casei Shirota strain with variation dose of 3.6 x 107, 7.3 x 107 and 10.9 x 107 cells/kg bodyweight/day. The results showed that probiotic of Lactobacillus casei Shirota strain therapy dose of 10.9 x 107 cells/kg bodyweight/day can decrease the total blood cholesterol of rats’ serum that were given with high-cholesterol diet of about 32.0%. In addition, the probiotic of Lactobacillus casei Shirota strain therapy also increase the activity of SOD about 50.70%.

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