Cap1p plays regulation roles in redox, energy metabolism and substance transport: an investigation on Candida albicans under normal culture condition

10.2741/2055 ◽  
2007 ◽  
Vol 12 (1) ◽  
pp. 145 ◽  
Author(s):  
Yan Wang
Keyword(s):  
Candida Albicans ◽  
Energy Metabolism ◽  
Culture Condition ◽  
Normal Culture ◽  
Normal Culture Condition ◽  
Substance Transport

scholarly journals Study on the Function of the Inositol Polyphosphate Kinases Kcs1 and Vip1 of Candida albicans in Energy Metabolism

2020 ◽  
Vol 11 ◽  
Author(s):  
Xueling Peng ◽  
Qilin Yu ◽  
Yingzheng Liu ◽  
Tianyu Ma ◽  
Mingchun Li
Keyword(s):  
Candida Albicans ◽  
Energy Metabolism ◽  
Cell Membrane ◽  
Cell Viability ◽  
Membrane Permeability ◽  
Cell Activity ◽  
Cell Membrane Permeability ◽  
Mitochondrial Activity ◽  
Inositol Polyphosphate ◽  
Growth Activity

In Saccharomyces cerevisiae, inositol polyphosphate kinase KCS1 but not VIP1 knockout is of great significance for maintaining cell viability, promoting glycolysis metabolism, and inducing mitochondrial damage. The functions of Candida albicans inositol polyphosphate kinases Kcs1 and Vip1 have not yet been studied. In this study, we found that the growth rate of C. albicans vip1Δ/Δ strain in glucose medium was reduced and the upregulation of glycolysis was accompanied by a decrease in mitochondrial activity, resulting in a large accumulation of lipid droplets, along with an increase in cell wall chitin and cell membrane permeability, eventually leading to cell death. Relieving intracellular glycolysis rate or increasing mitochondrial metabolism can reduce lipid droplet accumulation, causing a reduction in chitin content and cell membrane permeability. The growth activity and energy metabolism of the vip1Δ/Δ strains in a non-fermentable carbon source glycerol medium were not different from those of the wild-type strains, indicating that knocking out VIP1 did not cause mitochondria damage. Moreover, C. albicans KCS1 knockout did not affect cell activity and energy metabolism. Thus, in C. albicans, Vip1 is more important than Kcs1 in regulating cell viability and energy metabolism.

Influence of boric acid on energy metabolism and stress tolerance of Candida albicans

2018 ◽  
Vol 49 ◽  
pp. 140-145
Author(s):  
Martin Schmidt ◽  
Dominic Tran-Nguyen ◽  
Patrick Chizek
Keyword(s):  
Candida Albicans ◽  
Energy Metabolism ◽  
Boric Acid ◽  
Stress Tolerance

Candida albicans cells lacking CaMCA1-encoded metacaspase show resistance to oxidative stress-induced death and change in energy metabolism

2009 ◽  
Vol 46 (2) ◽  
pp. 183-189 ◽  
Author(s):  
YingYing Cao ◽  
Shan Huang ◽  
BaoDi Dai ◽  
ZhenYu Zhu ◽  
Hui Lu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Candida Albicans ◽  
Energy Metabolism

scholarly journals Inhibition of Respiration of Candida albicans by Small Molecules Increases Phagocytosis Efficacy by Macrophages

mSphere ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Shuna Cui ◽  
Minghui Li ◽  
Rabeay Y. A. Hassan ◽  
Anna Heintz-Buschart ◽  
Junsong Wang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Plasma Membrane ◽  
Energy Metabolism ◽  
Unsaturated Fatty Acids ◽  
Immune Defense ◽  
Host Cells ◽  
Wall Structure ◽  
Content Type ◽  
Metabolic Studies

ABSTRACT Candida albicans adapts to various conditions in different body niches by regulating gene expression, protein synthesis, and metabolic pathways. These adaptive reactions not only allow survival but also influence the interaction with host cells, which is governed by the composition and structure of the fungal cell wall. Numerous studies had shown linkages between mitochondrial functionality, cell wall integrity and structure, and pathogenicity. Thus, we decided to inhibit single complexes of the respiratory chain of C. albicans and to analyze the resultant interaction with macrophages via their phagocytic activity. Remarkably, inhibition of the fungal bc1 complex by antimycin A increased phagocytosis, which correlated with an increased accessibility of β-glucans. To contribute to mechanistic insights, we performed metabolic studies, which highlighted significant changes in the abundance of constituents of the plasma membrane. Collectively, our results reinforce the strong linkage between fungal energy metabolism and other components of fungal physiology, which also determine the vulnerability to immune defense reactions. IMPORTANCE The yeast Candida albicans is one of the major fungal human pathogens, for which new therapeutic approaches are required. We aimed at enhancements of the phagocytosis efficacy of macrophages by targeting the cell wall structure of C. albicans, as the coverage of the β-glucan layer by mannans is one of the immune escape mechanisms of the fungus. We unambiguously show that inhibition of the fungal bc1 complex correlates with increased accessibilities of β-glucans and improved phagocytosis efficiency. Metabolic studies proved not only the known direct effects on reactive oxygen species (ROS) production and fermentative pathways but also the clear downregulation of the ergosterol pathway and upregulation of unsaturated fatty acids. The changed composition of the plasma membrane could also influence the interaction with the overlying cell wall. Thus, our work highlights the far-reaching relevance of energy metabolism, indirectly also for host-pathogen interactions, without affecting viability.

Mitochondrial matrix calcium ions regulate energy production in myocardium: A cytochemical study

1990 ◽  
Vol 48 (2) ◽  
pp. 166-167
Author(s):  
W.A. Jacob ◽  
R. Hertsens ◽  
A. Van Bogaert ◽  
M. De Smet
Keyword(s):  
Energy Metabolism ◽  
Calcium Ions ◽  
Energy Production ◽  
Regulation Of Respiration ◽  
Free Calcium ◽  
Mitochondrial Matrix ◽  
Cytochemical Study ◽  
Nmr Techniques

In the past most studies of the control of energy metabolism focus on the role of the phosphorylation potential ATP/ADP.Pi on the regulation of respiration. Studies using NMR techniques have demonstrated that the concentrations of these compounds for oxidation phosphorylation do not change appreciably throughout the cardiac cycle and during increases in cardiac work. Hence regulation of energy production by calcium ions, present in the mitochondrial matrix, has been the object of a number of recent studies.Three exclusively intramitochondnal dehydrogenases are key enzymes for the regulation of oxidative metabolism. They are activated by calcium ions in the low micromolar range. Since, however, earlier estimates of the intramitochondnal calcium, based on equilibrium thermodynamic considerations, were in the millimolar range, a physiological correlation was not evident. The introduction of calcium-sensitive probes fura-2 and indo-1 made monitoring of free calcium during changing energy metabolism possible. These studies were performed on isolated mitochondria and extrapolation to the in vivo situation is more or less speculative.

Enhanced IgE response to Candida albicans in postoperative invasive candidiasis

1996 ◽  
Vol 26 (4) ◽  
pp. 452-460 ◽  
Author(s):  
J. SAVOLAINEN ◽  
A. RANTALA ◽  
M. NERMES ◽  
L. LEHTONEN ◽  
M. VIANDER
Keyword(s):  
Candida Albicans ◽  
Invasive Candidiasis ◽  
Ige Response

Existence of Candida albicans and microorganisms in denture stomatitis patients

1997 ◽  
Vol 24 (10) ◽  
pp. 788-790 ◽  
Author(s):  
Y. KULAK ◽  
A. ARIKAN ◽  
E. KAZAZOGLU
Keyword(s):  
Candida Albicans ◽  
Denture Stomatitis

Importance des micromycètes dans le microbiote intestinal : le modèle Candida albicans.

2018 ◽  
Vol 202 (7) ◽  
pp. 1401-1412
Author(s):  
Alain Bonnin ◽  
Frédéric Dalle
Keyword(s):  
Candida Albicans ◽  
Microbiote Intestinal
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