Shotgun proteomics analysis on maize chloroplast thylakoid membrane

10.2741/e240 ◽  
2011 ◽  
Vol E3 (1) ◽  
pp. 250-255 ◽  
Author(s):  
Bai-Chen Wang
Keyword(s):  
Thylakoid Membrane ◽  
Shotgun Proteomics ◽  
Proteomics Analysis ◽  
Maize Chloroplast

scholarly journals An Improvement of Shotgun Proteomics Analysis by Adding Next-Generation Sequencing Transcriptome Data in Orange

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39494 ◽  
Author(s):  
Jiaping Song ◽  
Renjie Sun ◽  
Dazhi Li ◽  
Fengji Tan ◽  
Xin Li ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Shotgun Proteomics ◽  
Transcriptome Data ◽  
Next Generation ◽  
Proteomics Analysis ◽  
Generation Sequencing

scholarly journals Shotgun Proteomics Analysis of Saliva and Salivary Gland Tissue from the Common Octopus Octopus vulgaris

2018 ◽  
Vol 17 (11) ◽  
pp. 3866-3876 ◽  
Author(s):  
Legana C. H. W. Fingerhut ◽  
Jan M. Strugnell ◽  
Pierre Faou ◽  
Álvaro Roura Labiaga ◽  
Jia Zhang ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Shotgun Proteomics ◽  
Octopus Vulgaris ◽  
Proteomics Analysis ◽  
Salivary Gland Tissue ◽  
Gland Tissue ◽  
The Common ◽  
Common Octopus

Shotgun proteomics analysis of proliferating STRO-1-positive human dental pulp cell after exposure to nacreous water-soluble matrix

2014 ◽  
Vol 19 (2) ◽  
pp. 261-270 ◽  
Author(s):  
Titikan Laothumthut ◽  
Jeeraphat Jantarat ◽  
Atchara Paemanee ◽  
Sittiruk Roytrakul ◽  
Panjit Chunhabundit
Keyword(s):  
Dental Pulp ◽  
Shotgun Proteomics ◽  
Water Soluble ◽  
Proteomics Analysis ◽  
Pulp Cell ◽  
Human Dental Pulp Cell ◽  
Dental Pulp Cell ◽  
Human Dental Pulp

scholarly journals Streamlined Membrane Proteome Preparation for Shotgun Proteomics Analysis with Triton X-100 Cloud Point Extraction and Nanodiamond Solid Phase Extraction

Materials ◽  
2016 ◽  
Vol 9 (5) ◽  
pp. 385 ◽  
Author(s):  
Minh Pham ◽  
Ting-Chun Wen ◽  
Hung-Cheng Li ◽  
Pei-Hsuan Hsieh ◽  
Yet-Ran Chen ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Cloud Point ◽  
Cloud Point Extraction ◽  
Solid Phase ◽  
Shotgun Proteomics ◽  
Phase Extraction ◽  
Membrane Proteome ◽  
Proteomics Analysis ◽  
Triton X

Optimizing Shotgun Proteomics Analysis for a Confident Protein Identification and Quantitation in Orphan Plant Species: The Case of Holm Oak (Quercus ilex)

2020 ◽  
pp. 157-168
Author(s):  
Isabel Gómez-Gálvez ◽  
Rosa Sánchez-Lucas ◽  
Bonoso San-Eufrasio ◽  
Luis Enrique Rodríguez de Francisco ◽  
Ana M. Maldonado-Alconada ◽  
...  
Keyword(s):  
Plant Species ◽  
Protein Identification ◽  
Quercus Ilex ◽  
Shotgun Proteomics ◽  
Holm Oak ◽  
Proteomics Analysis ◽  
Protein Identification And Quantitation

Shotgun proteomics analysis reveals new unsuspected molecular effectors of nitrogen-containing bisphosphonates in osteocytes

2011 ◽  
Vol 74 (7) ◽  
pp. 1113-1122 ◽  
Author(s):  
Nicoletta Bivi ◽  
Paola Picotti ◽  
Lukas N. Müller ◽  
Milena Romanello ◽  
Luigi Moro ◽  
...  
Keyword(s):  
Shotgun Proteomics ◽  
Proteomics Analysis

scholarly journals An improved deyolking procedure facilitates the proteomics analysis of early developmental stages of zebrafish embryos

2019 ◽  
Author(s):  
Purushothaman Kathiresan ◽  
Christopher Presslauer ◽  
Prem Prakash Das ◽  
Lim Teck Kwang ◽  
Steinar Dae Johansen ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Cell Cycle Control ◽  
Fold Increase ◽  
Shotgun Proteomics ◽  
Egg Yolk ◽  
Vertebrate Development ◽  
Proteomics Analysis ◽  
Early Embryos ◽  
Mitochondrial Functions ◽  
Innovative Tool

Abstract Background: Zebrafish is a well-recognised organism for investigating vertebrate development and human diseases. However, the data on zebrafish proteome are scarce, particularly during embryogenesis. This is mostly due to the overwhelming abundance of egg yolk proteins, which tend to mask the detectable presence of less abundant proteins. Results: In this study, we developed an efficient procedure to reduce the amount of yolk in zebrafish early embryos to improve the LC-MS-based shotgun proteomics analysis. We demonstrated that the deyolking procedure resulted in a greater number of proteins being identified. This protocol resulted in approximately two-fold increase in the number of proteins identified in deyolked samples at cleavage stages, and the number of identified proteins increased greatly by 3-4 times compared to non-deyolked samples in both oblong and bud stages. Gene Ontology and KEGG analysis revealed a high number of functional proteins differentially accumulated in the deyolked versus non-deyolked samples. The most prominent enrichments after the deyolking procedure included processes, functions and components related to cellular organization, cell cycle, control of replication and translation, and mitochondrial functions. Conclusion: This deyolking procedure improves both qualitative and quantitative proteome analyses and provides an innovative tool in molecular embryogenesis of polylecithal animals, such as fish, amphibians, reptiles, or birds.

A label-free shotgun proteomics analysis of macadamia nut

2020 ◽  
Vol 129 ◽  
pp. 108838
Author(s):  
Johanna Rost ◽  
Sridevi Muralidharan ◽  
N. Alice Lee
Keyword(s):  
Shotgun Proteomics ◽  
Label Free ◽  
Proteomics Analysis ◽  
Macadamia Nut
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