PARKINSONIA ACULEATA AS A POTENTIAL PHYTOTHERAPIC FACTOR IN THE INFLAMMATORY AND DYSLIPIDEMIC CONTROL OF WISTAR RATS

2021 ◽  
pp. 8
Author(s):  
◽  
◽  
◽  
Keyword(s):  
Inflammatory Cytokines ◽  
Wistar Rats ◽  
Serum Triglycerides ◽  
Hydroalcoholic Extract ◽  
Parkinsonia Aculeata ◽  
Commercial Diet ◽  
Aerial Parts ◽  
Westernized Diet ◽  
Male Wistar Rats ◽  
Pro Inflammatory Cytokines

Objective: The study evaluated the hypolipidemic and anti-inflammatory potential of the hydroalcoholic extract of P. Aculeata (HEPa/EtOAC) in Wistar rats fed with Westernized diet. Methods: The experiment was approved by the Ethics Committee (Federal University of Pernambuco-23076.027165 / 2010-21). The hydroalcoholic extract (HEPa/EtOAc) was obtained from the aerial parts of P. Aculeata and portioned by acetate/ethanol in different concentrations. 36 male Wistar rats were initially divided into two groups: Control (C, n = 6), fed a commercial diet (26g / 100g of protein; 63g / 100g of carbohydrates; 11g / 100g of lipids) and Westernized (W; n = 30) – fed an experimental diet (15g / 100g of protein; 51g / 100g of carbohydrates; 34 g/100g of lipids) receving water ad libitum. After 4 months of diet, W group was arbitrarily redistributed into six subgroups according to water supplementation, or different extracts concentrations: (n = 6 animals/group): W- distilled water (1mL/kg; po); WG – genfibrozil (140 mg/kg; p.o.); W35- 35 mg/kg; W70 – 70 mg /kg; p.o. and W140- 140 mg/kg; p.o for 30 days. The lipid profile and pro-inflammatory cytokines were analyzed. Results: The westernized diet caused dyslipidemia and the treatment with HEPa-EtOAc (140mg / kg), promoted a significant reduction in the concentration of pro-inflammatory cytokines, in total cholesterol, in the LDLc and serum triglycerides. Conclusion: The reduction of dyslipidemia and pro-inflammatory cytokines from the of P. Aculeata extract corroborate the ethnopharmacological potential of the plant, already used by the population for hypoglycemic purposes.

scholarly journals Protective Effect of Terminalia arjuna Against DBTC Induced Pancreatic Cancer in Male Wistar Rats

2021 ◽  
pp. 248-256
Author(s):  
Kadiyala Harshitha ◽  
Uma Sankar Gorla
Keyword(s):  
Pancreatic Cancer ◽  
Body Weight ◽  
Protective Effect ◽  
Wistar Rats ◽  
Negative Control ◽  
Terminalia Arjuna ◽  
Hydroalcoholic Extract ◽  
Group I ◽  
Male Wistar Rats ◽  
Per Oral

Aims: To study the protective effect of hydroalcoholic bark extract of Terminalia arjuna against DBTC induced pancreatic cancer in male wistar rats. Study design: Healthy male Wistar Albino rats weighing 150-200 g were segregated into four groups (n=6). Group I was considered as normal control, received normal saline (0.9%w/v, 1 ml/kg body weight, orally). Group II rats were treated with DBTC (6 mg/kg body weight, i.p.) which served as negative control. Group III and IV received Terminalia arjuna Linn bark hydroalcoholic extract at doses of 250 mg/kg body weight, per oral and 500 mg/kg body weight, per oral respectively. Place and Duration of Study: University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India, between May 2020 and July 2020. Methodology: The experimental animals were segregated into four groups of six rats each. According to acute toxicity data, 250 mg/kg as low dose and 500 mg/kg as high dose of the test compound have been chosen for administration. All the drugs were given for 28 consecutive days to all the respective groups with standard pellet diet and water ad libitum. The assessment of serum parameters such as α-Amylase, Lipase and blood glucose levels were carried out on 1st day, 14th day and 28th day to the respective groups. Results: Pretreated groups of Terminalia arjuna Linn bark hydroalcoholic extract (250 mg/kg and 500 mg/kg body weight, orally) showed significant (‘#’p<0.001) decrease in the levels of α-Amylase, Lipase and glucose in the blood when compared to DBTC (6 mg/kg body weight, i.p.) induced group which served as negative control. Conclusion: This study suggests that Terminalia arjuna may have a protective role against DBTC induced pancreatic cancer in male wistar rats and further investigation may be required to confirm its therapeutic potentials clinically.

scholarly journals Mitigation of Arsenic-induced Increases in Pro-Inflammatory Cytokines and Haematological Derangements by Ethanol Leaf Extract of Irvingia gabonensis

2020 ◽  
pp. 36-47
Author(s):  
Efosa Godwin Ewere ◽  
Ngozi Paulinus Okolie ◽  
Oboso Edem Etim ◽  
Samson Adewale Oyebadejo
Keyword(s):  
Blood Cell ◽  
Inflammatory Cytokines ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Haemoglobin Concentration ◽  
Interleukin 4 ◽  
Concomitant Treatment ◽  
Tnf Α ◽  
Irvingia Gabonensis ◽  
Pro Inflammatory Cytokines

Aim: To investigate the effect of ethanol leaf extract of Irvingia gabonensis (ELEIG) on sodium arsenite (SA)-induced hepatic pro-inflammatory cytokines and haematological derangements in Wistar rats. Study Design: Fifty five Wistar rats weighing between 100 g and 161 g were randomly distributed to eleven (11) groups (n=5). Group 1 (control) had feed and water only. Group 2 received SA at a dose of 4.1 mg/kg body weight (kgbw) for 14 days. Groups 3-11 received ELEIG with or without SA. Place and Duration of Study: Biochemistry Department, University of Uyo and Biochemistry Department, University of Benin. The duration of the study was 14 days. Methodology: Treatment was done orally for 14 days. Immunological markers: tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-4 (IL-4), interleukin-10 (IL-10), and haematological indices: red blood cell (RBC) count, haemoglobin (HB) concentration, packed cell volume (PCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC), mean corpuscular volume (MCV), white blood cell (WBC) count and its differentials and platelet (PLT) count were used to determine the immunomodulatory and haematological effects of the extract. Results: Intoxication with SA caused significant (p ˂ 0.05) increases in hepatic TNF-α, IL-1β, and IL-4 levels and a significant decrease in hepatic IL-10 level, relative to control. The SA treatment also caused significant (p ˂ 0.05) decreases in RBC, HB, PCV MCH, MCHC, MCV, PLT and monocyte counts as well as significant (p ˂ 0.05) increases in WBC, lymphocyte, basophil, eosinophil and neutrophil counts, relative to control. However, post-treatment and concomitant treatment with ELEIG ameliorated the noxious effect of SA. In addition, ELEIG alone at various doses produced results with most of the assayed parameters having values comparable with those control. Conclusion: These results indicate that ELEIG mitigates SA-induced inflammation and haematological perturbations in Wistar rats.

scholarly journals Effect of Hydroalcoholic Extract ofCydonia oblongaMiller (Quince) on Sexual Behaviour of Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Muhammad Aslam ◽  
Ali Akbar Sial
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Sexual Behaviour ◽  
Wistar Rats ◽  
Sexually Active ◽  
Cydonia Oblonga ◽  
Hydroalcoholic Extract ◽  
Male Wistar Rats ◽  
Mating Performance ◽  
Remarkable Manner

Cydonia oblongaMiller (quince) is regarded as a potent libido invigorator in Tib-e-Nabvi and Unani System of Medicine. This study was carried out to evaluate the aphrodisiac activity of the hydroalcoholic extract of the fruits ofCydonia oblongaMiller (quince) in Wistar rats. The extract was administered orally by gavage in the dose of 500 mg/kg and 800 mg/kg body weight per day as a single dose for 28 days. The observed parameters were mounting frequency, assessment of mating performance, and orientation activities towards females, towards the environment, and towards self. The results showed that after administration of the extract mounting frequency and the mating performance of the rats increased highly significantlyP<0.01. The extract also influenced the behaviour of treated animals in comparison to nontreated rats in a remarkable manner, making them more attracted to females. These effects were observed in sexually active male Wistar rats.

Protective effect of Ferulago angulata (Schltdl.) Boiss. hydroalcoholic extract against acetic acid-induced colitis in rat: Role of pro-inflammatory cytokines

2018 ◽  
Vol 10 (4) ◽  
pp. 391
Author(s):  
MohammadHosein Farzaei ◽  
Fatemeh Farzaei ◽  
Fatemeh Heydarpour ◽  
ZahraMinush Siavash Haghighi ◽  
Zahra Abbasabadi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Protective Effect ◽  
Inflammatory Cytokines ◽  
Hydroalcoholic Extract ◽  
Pro Inflammatory Cytokines

scholarly journals Beneficial Effect of Chronic Treatment with Extracts from Rhodiola Rosea L. and Curcuma Longa L. on the Immunoreactivity of Animals Subjected to a Chronic Mild Stress Model

Folia Medica ◽  
2017 ◽  
Vol 59 (4) ◽  
pp. 443-453 ◽  
Author(s):  
Liliya V. Vasileva ◽  
Kremena Е. Saracheva ◽  
Mariya V. Ivanovska ◽  
Atanaska P. Petrova ◽  
Emir Sucouglu ◽  
...  
Keyword(s):  
Weight Gain ◽  
Locomotor Activity ◽  
Inflammatory Cytokines ◽  
Curcuma Longa ◽  
Chronic Mild Stress ◽  
Mild Stress ◽  
Model Group ◽  
Tnf Α ◽  
Male Wistar Rats ◽  
Pro Inflammatory Cytokines

AbstractBackground: Recent studies have suggested increased levels of pro-inflammatory cytokines in depression. Aim: The present study aimed to evaluate the effect of extracts from Rhodiola and Curcuma on immunoreactivity of animals subjected to a chronic mild stress (CMS) model followed by lipopolysaccharide-induced inflammation. Materials and methods: Male Wistar rats (n=56) divided in 7 groups were treated orally with: distilled water 10 ml/kg (control and CMS model groups); Rhodiola 250 mg/kg; Rhodiola 500 mg/kg; Curcuma 250 mg/kg; Curcuma 500 mg/kg, Rhodiola 250 mg/kg and Curcuma 250 mg/kg. All groups except the control were stressed daily according to a CMS protocol. Changes in glucose preference, weight gain and locomotor activity were recorded. In the sixth week the animals were challenged with LPS and rats’ sera were obtained for ELISA evaluation of TNF-α and IL-6 levels. Results: The animals from the model group decreased their weight gain, glucose preference and locomotor activity compared to controls. The groups exposed to stress and treated with Rhodiola 500 mg/kg, Curcuma 500 mg/kg and their combination increased their locomotor activity compared to the model group. High expression of the pro-inflammatory cytokines TNF-α and IL-6 were found in all groups exposed to CMS and challenged by LPS. Conclusions: The groups exposed to the stress procedure showed a variety of depression-like behavioral changes. In addition, ELISA tests showed that CMS is affecting rats’ immunity by increasing the cytokines’ levels. These changes could be reversed by administration of Rhodiola and Curcuma in combination suggesting synergic interaction regarding their anti-inflammatory and anti-stress effects.

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