scholarly journals The “embryo in planta – callus in vitro” system: cytophysiological aspects (by wheat example)

2020 ◽  
Author(s):  
N. N. Kruglova ◽  
O. A. Seldimirova
Keyword(s):  
Plant Regeneration ◽  
Immature Embryos ◽  
In Planta ◽  
In Vitro System

Plant regeneration from calli in vitro is an integral part of a number of biotechnologies. Immature embryos (IE) are particularly promising as explants for obtaining morphogenic calli (MC) in cereals.

scholarly journals Plant regeneration in vitro from immature embryos of lesser burnet (Sanguisorba minor Scop.)

2003 ◽  
Vol 69 (3) ◽  
pp. 446-447
Author(s):  
S. Çöçü ◽  
K.M. Khawar ◽  
C. Sancak ◽  
Sebahattin Özcan ◽  
C.H. Bornman ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Immature Embryos ◽  
Regeneration In Vitro ◽  
Sanguisorba Minor

scholarly journals Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.)

2018 ◽  
Vol 59 (0) ◽  
Author(s):  
Roghayeh Ahmadpour ◽  
Nasser Zare ◽  
Rasool Asghari-Zakarta ◽  
Parisa Sheikhzadeh
Keyword(s):  
Triticum Aestivum ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Triticum Aestivum L ◽  
Immature Embryos

Acquisition of desiccation tolerance in developing oil palm (Elaeis guineensis Jacq.) embryos in planta and in vitro in relation to sugar content

2003 ◽  
Vol 13 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Frédérique Aberlenc-Bertossi ◽  
Nathalie Chabrillange ◽  
Françoise Corbineau ◽  
Yves Duval
Keyword(s):  
Moisture Content ◽  
Oil Palm ◽  
Desiccation Tolerance ◽  
Dry Matter ◽  
Elaeis Guineensis ◽  
Dry Weight ◽  
Immature Embryos ◽  
In Planta ◽  
High Sucrose

AbstractRelationships between desiccation tolerance and dry matter, water and sugar contents were studied throughout the development of oil palm (Elaeis guineensis Jacq.) zygotic embryos and in immature embryos cultured on a sucrose-enriched medium. Embryo dry weight during in planta development increased between 80 and 140 d after pollination (DAP) and was then stable until maturity. Embryos underwent dehydration until 120 DAP, but their moisture content remained high at maturity (c. 2 g H2O g-1 DW). Desiccation tolerance was acquired between 83 and 104 DAP, and was positively correlated with embryo age and dry weight, and negatively correlated with initial water content during this period. Sucrose, the main soluble sugar present throughout embryo development, accounted for an average of 24% of the dry weight. Glucose and fructose contents decreased to less than 1 mg g-1 DW in embryos at maturity. At 117 DAP, as embryos became tolerant to desiccation, the monosaccharides/sucrose ratio fell to 0.015 and raffinose was detected. Stachyose appeared later in 147-day-old embryos and accumulated until shedding. In vitro culture of immature embryos in the presence of high sucrose concentrations (350 and 700 mM) resulted in an increase in their dry weight and a decrease in their water content, and induced the acquisition of desiccation tolerance. Under these conditions, sucrose accumulated in embryos to 30–40% on a dry weight basis, but neither raffinose nor stachyose was detected. Acquisition of desiccation tolerance by oil palm immature embryos was associated both in planta and in vitro with an accumulation of dry matter, a reduction of moisture content, and a fall in the monosaccharides/sucrose ratio. In planta, survival to dehydration was also related with the deposition of oligosaccharides whereas in vitro, it was related with high sucrose accumulation. The role of sugars in the acquisition of desiccation tolerance in oil palm embryos is discussed.

scholarly journals Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

2014 ◽  
Vol 38 ◽  
pp. 348-353 ◽  
Author(s):  
Satı UZUN ◽  
Ali İrfan İLBAŞ ◽  
Arif İPEK ◽  
Neşet ARSLAN ◽  
Surendra BARPETE
Keyword(s):  
Plant Regeneration ◽  
Immature Embryos ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant

The “embryo in planta–callus in vitro” system: Cytophysiological aspects (by example of wheat)

Biomics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 180-189
Author(s):  
N.N. Kruglova ◽  
O.A. Seldimirova
Keyword(s):  
In Planta ◽  
In Vitro System

scholarly journals Callus induction and plant regeneration by Brazilian triticale and wheat genotypes

1997 ◽  
Vol 20 (1) ◽  
pp. 41-44 ◽  
Author(s):  
A.L.C. Dornelles ◽  
F.I.F. Carvalho ◽  
L.C. Federizzi ◽  
C.L. Handel ◽  
F. Bered ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Culture Media ◽  
Rio Grande ◽  
Immature Embryos ◽  
Ms Medium ◽  
Rio Grande Do Sul ◽  
Wheat Genotypes ◽  
The One

In order to determine the in vitro behavior of Brazilian triticale, 16 triticale genotypes, and three wheat genotypes used as checks, were sown in June 1994. The explants used were immature embryos. In addition to the genotype tests, two culture media for callus induction were also evaluated, i.e., MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) medium containing 2.0 mg 2,4D/l, and MS medium containing 4.0 mg 2,4D/l. The plant regeneration protocol used was the one employed at the Laboratório de Cultura de Tecidos, Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul, for wheat. Differences in plant regeneration were observed both among triticale and wheat genotypes, with triticale usually showing better regeneration than wheat. No differences were observed between the callus induction media.

Somatic embryogenesis and plant regeneration from immature embryos of Carica papaya × Carica cauliflora cultured in vitro

1991 ◽  
Vol 69 (9) ◽  
pp. 1913-1918 ◽  
Author(s):  
M. H. Chen ◽  
C. C. Chen ◽  
D. N. Wang ◽  
F. C. Chen
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Interspecific Hybrids ◽  
Carica Papaya ◽  
Immature Embryos ◽  
Embryogenic Cultures ◽  
Regenerated Plants ◽  
Hybrid Embryo

Somatic embryos were induced directly on immature embryos of Carica papaya × Carica cauliflora hybrids cultured on modified Murashige and Skoog's medium. When transferred to medium supplemented with abscisic acid, individual somatic embryos proliferated numerous daughter embryos through repeated embryogenesis. Light microscopic study of the repeatedly embryogenic cultures showed that daughter embryos arose from single superficial cells of parent embryos. Plant regeneration occurred following transfer of somatic embryos to medium devoid of plant growth regulators. Regenerated plants were intermediate between C. papaya and C. cauliflora in several morphological respects and showed isozyme patterns specific to both species as well as some new bands, indicating that they are indeed interspecific hybrids. Key words: Carica, interspecific hybrid, embryo culture, somatic embryogenesis.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

The Mesothelial Cell as a Non-Thrombogenic Surface

1984 ◽  
Vol 52 (02) ◽  
pp. 102-104 ◽  
Author(s):  
L J Nicholson ◽  
J M F Clarke ◽  
R M Pittilo ◽  
S J Machin ◽  
N Woolf
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Flow ◽  
Cell Surface ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Plastic Film ◽  
In Vitro System ◽  
Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

Sign in / Sign up

Export Citation Format

Share Document