Acquisition of desiccation tolerance in developing oil palm (Elaeis guineensis Jacq.) embryos in planta and in vitro in relation to sugar content

2003 ◽  
Vol 13 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Frédérique Aberlenc-Bertossi ◽  
Nathalie Chabrillange ◽  
Françoise Corbineau ◽  
Yves Duval
Keyword(s):  
Moisture Content ◽  
Oil Palm ◽  
Desiccation Tolerance ◽  
Dry Matter ◽  
Elaeis Guineensis ◽  
Dry Weight ◽  
Immature Embryos ◽  
In Planta ◽  
High Sucrose

AbstractRelationships between desiccation tolerance and dry matter, water and sugar contents were studied throughout the development of oil palm (Elaeis guineensis Jacq.) zygotic embryos and in immature embryos cultured on a sucrose-enriched medium. Embryo dry weight during in planta development increased between 80 and 140 d after pollination (DAP) and was then stable until maturity. Embryos underwent dehydration until 120 DAP, but their moisture content remained high at maturity (c. 2 g H2O g-1 DW). Desiccation tolerance was acquired between 83 and 104 DAP, and was positively correlated with embryo age and dry weight, and negatively correlated with initial water content during this period. Sucrose, the main soluble sugar present throughout embryo development, accounted for an average of 24% of the dry weight. Glucose and fructose contents decreased to less than 1 mg g-1 DW in embryos at maturity. At 117 DAP, as embryos became tolerant to desiccation, the monosaccharides/sucrose ratio fell to 0.015 and raffinose was detected. Stachyose appeared later in 147-day-old embryos and accumulated until shedding. In vitro culture of immature embryos in the presence of high sucrose concentrations (350 and 700 mM) resulted in an increase in their dry weight and a decrease in their water content, and induced the acquisition of desiccation tolerance. Under these conditions, sucrose accumulated in embryos to 30–40% on a dry weight basis, but neither raffinose nor stachyose was detected. Acquisition of desiccation tolerance by oil palm immature embryos was associated both in planta and in vitro with an accumulation of dry matter, a reduction of moisture content, and a fall in the monosaccharides/sucrose ratio. In planta, survival to dehydration was also related with the deposition of oligosaccharides whereas in vitro, it was related with high sucrose accumulation. The role of sugars in the acquisition of desiccation tolerance in oil palm embryos is discussed.

scholarly journals Accelerated in vitro propagation of elite oil palm genotypes (Elaeis guineensis Jacq.) by substituting cytokinin with putrescine

2016 ◽  
Vol 15 (50) ◽  
pp. 2767-2775 ◽  
Author(s):  
Roseli Correa Thais ◽  
Yoshimitsu Motoike Sérgio ◽  
Paula de Souza Andrade Ana ◽  
Morra Coser Sara ◽  
Queiroz Vanessa ◽  
...  
Keyword(s):  
Oil Palm ◽  
In Vitro Propagation ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

Prevention and Detection of Fungicide Resistance Development in Rhizoctonia zeae from Soybean and Corn in Nebraska

2021 ◽  
Author(s):  
Nikita Gambhir ◽  
Srikanth Kodati ◽  
Matthew Huff ◽  
Flávio Silva ◽  
Olutoyosi Ajayi-Oyetunde ◽  
...  
Keyword(s):  
Fungicide Resistance ◽  
Resistance Management ◽  
Dry Weight ◽  
Geographic Region ◽  
Population Characteristics ◽  
Current Status ◽  
In Planta ◽  
Resistance Development ◽  
Rhizoctonia Zeae

The goal of this research was to advance the foundational knowledge required to quantify and mitigate fungicide resistance in Rhizoctonia zeae, the seedling disease pathogen of soybean and corn. In vitro sensitivity to azoxystrobin, fludioxonil, sedaxane, and/or prothioconazole was determined for 91 R. zeae isolates obtained mostly from soybean and corn fields in Nebraska. Isolates were sensitive to fludioxonil, sedaxane, and prothioconazole (EC50 < 3 µg/ml) and had a positively skewed EC50 distribution. Isolates were not sensitive to azoxystrobin in vitro (EC50 > 100 µg/ml) or in planta. Application of azoxystrobin did not significantly decrease disease severity or improve total dry weight of the soybean plants (P > 0.05). The risk of resistance development in R. zeae was estimated by characterizing its population structure. Eighty-one R. zeae isolates were genotyped using six microsatellite markers. Results showed that the population has a mixed mode of reproduction and is structured according to geographic region, suggesting limited dispersal. These population characteristics suggest that R. zeae has an intermediate risk of resistance development. Overall, this research established the current status of fungicide sensitivity in R. zeae in Nebraska and estimated its risk of resistance development, which can inform fungicide resistance management for R. zeae.

Seed development in Malva parviflora: onset of germinability, dormancy and desiccation tolerance

2007 ◽  
Vol 47 (6) ◽  
pp. 683 ◽  
Author(s):  
Pippa J. Michael ◽  
Kathryn J. Steadman ◽  
Julie A. Plummer
Keyword(s):  
Moisture Content ◽  
Seed Development ◽  
Desiccation Tolerance ◽  
Dry Weight ◽  
Physical Dormancy ◽  
Seed Moisture Content ◽  
Physiological Maturity ◽  
Seed Moisture ◽  
Physiological Dormancy ◽  
Malva Parviflora

Seed development was examined in Malva parviflora. The first flower opened 51 days after germination; flowers were tagged on the day that they opened and monitored for 33 days. Seeds were collected at 12 stages during this period and used to determine moisture content, germination of fresh seeds and desiccation tolerance (seeds dried to 10% moisture content followed by germination testing). Seed moisture content decreased as seeds developed, whereas fresh (max. 296 mg) and dry weight (max. 212 mg) increased to peak at 12–15 and ~21 days after flowering (DAF), respectively. Therefore, physiological maturity occurred at 21 DAF, when seed moisture content was 16–21%. Seeds were capable of germinating early in development, reaching a maximum of 63% at 9 DAF, but germination declined as development continued, presumably due to the imposition of physiological dormancy. Physical dormancy developed at or after physiological maturity, once seed moisture content declined below 20%. Seeds were able to tolerate desiccation from 18 DAF; desiccation hastened development of physical dormancy and improved germination. These results provide important information regarding M. parviflora seed development, which will ultimately improve weed control techniques aimed at preventing seed set and further additions to the seed bank.

scholarly journals Respons Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Pemberian Pupuk Organik Asal Pelepah Kelapa Sawit dan Pupuk Majemuk NPK

Agrikultura ◽  
2017 ◽  
Vol 28 (2) ◽  
Author(s):  
Mira Ariyanti ◽  
Gita Natali ◽  
Cucu Suherman
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Block Design ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Organic Fertilizers ◽  
Compound Fertilizer ◽  
Elaeis Guineensis Jacq ◽  
Four Levels ◽  
Palm Fronds

ABSTRACTThe growth response of oil palm (Elaeis Guineensis Jacq.) seedling toward the application of organic fertilizer from palm fronds and NPK compound fertilizerThe research was aimed to study the influence between organic fertilizers from palm fronds and NPK compound fertilizer to reduce NPK compound fertilizer in main nursery. The experiment was conducted from January to April 2017 at the Experiment Station Ciparanje, Faculty of Agriculture, Universitas Padjadjaran. The experimental design used was Randomized Block Design that arranged in factorial patterns with two factors and three replications. The fisrt factor was dosage of organic fertilizers from palm fronds consisted of three levels of 0 g/polybag, 800 g/polybag, and 1600 g/polybag and the second factor was dosage of NPK compound fertilizer consisted of four levels of 0 g/polybag, 20 g/polybag, 40 g/polybag, and 60 g/polybag. The result of the experiment showed that there was interaction effect between organic fertilizers from palm fronds and NPK compound fertilizer on height of seedling and dry weight of the shoot. The dosage of 1600 g/polybag organic fertilizers from palm fronds with the dosage of 20 g/polybag NPK compound fertilizer showed the best result in dry weight of the shoot.Keywords: Oil palm seedling, Main nursery, Organic fertilizer, Palm frond, NPK compound fertilizerABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh interaksi antara pupuk organik asal pelepah kelapa sawit dengan pupuk majemuk NPK yang baik untuk mengurangi penggunaan pupuk majemuk NPK di pembibitan utama kelapa sawit. Percobaan dilaksanakan dari bulan Januari sampai dengan April 2017 di Kebun Percobaan Ciparanje, Fakultas , Universitas Padjadjaran. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok dengan pola faktorial yang diulang sebanyak tiga kali. Faktor pertama meliputi dosis pupuk organik asal pelepah kelapa sawit terdiri dari tiga taraf yaitu 0 g/polybag, 800 g/polybag, dan 1600 g/polybag dan faktor kedua dosis pupuk majemuk NPK yang terdiri empat taraf yaitu 0 g/polybag, 20 g/polybag, 40 g/polybag, dan 60 g/polybag. Hasil percobaan menunjukkan bahwa terdapat pengaruh interaksi pupuk organik asal pelepah kelapa sawit dengan pupuk majemuk NPK terhadap tinggi tanaman dan bobot kering tajuk. Perlakuan pupuk organik asal pelepah kelapa sawit 1600 g/bibit dengan pupuk majemuk NPK 20 g/bibit menghasilkan bobot kering tajuk bibit kelapa sawit terbaik.Kata Kunci: Bibit kelapa sawit, Pembibitan utama, Pupuk organik, Pelepah kelapa sawit, NPK

scholarly journals Productivity of Indigofera zollingeriana under different canopy and soil acidity level in oil palm estate

2016 ◽  
Vol 21 (2) ◽  
pp. 135
Author(s):  
Iwan Herdiawan
Keyword(s):  
Organic Matter ◽  
Oil Palm ◽  
Dry Matter ◽  
Soil Acidity ◽  
Acid Soil ◽  
Block Design ◽  
Calcium Content ◽  
Great Opportunity ◽  
Dry Matter Digestibility

<p class="abstrak2">Oil palm estate area in Indonesia is generally located in a sub-optimal land that has great opportunity for the development of forage supply. This study aims were to determine productivity of <em>Indigofera</em> zollingeriana under various canopy level. This research used factorial randomized block design with 3 canopy levels (under 2, 5, and 7 year oil palm canopy) and 2 levels of soil acidity (neutral and acid soil) treatments, where each treatment was repeated 4 times. Parameters observed were production and nutrient content of <em>Indigofera</em> zollingeriana. Research results showed that there was no interaction between the canopy levels and soil acidity on the production of fresh leaves, stems/branches, biomass, and leaves/stem ratio of I. zollingeriana. Production of fresh leaves, stems, biomass, and leaves/branches ratio of <em>I. </em>zollingeriana significantly (P &lt;0.01) decreased along with increase of canopy level. Soil acidity significantly (P &lt;0.05) decreased production of fresh leaves, stems, biomass, and leaves/branches ratio. Level of canopy treatment significantly (P &lt;0.05) increased content of crude protein, crude fiber and energy, otherwise value of in vitro dry matter digestibility (IVDMD) and organic matter digestibilyity (IVOMD) were decrease. Soil acidity significantly (P &lt;0.05) decreased calcium content, in vitro dry matter digestibility (IVDMD) and organic matter digestibility (IVOMD) of <em>I. </em>zollingeriana.</p>

RELATIONSHIPS AMONG WHOLE-PLANT MOISTURE, GRAIN MOISTURE, DRY MATTER YIELD, AND QUALITY OF WHOLE-PLANT CORN SILAGE

1975 ◽  
Vol 55 (1) ◽  
pp. 77-84 ◽  
Author(s):  
T. B. DAYNARD ◽  
R. B. HUNTER
Keyword(s):  
Moisture Content ◽  
Dry Matter ◽  
Yield Potential ◽  
In Vitro Digestibility ◽  
Research Station ◽  
Whole Plant ◽  
Grain Moisture ◽  
Plant Moisture ◽  
Plant Moisture Content

Identical experiments were conducted at the Elora Research Station, near Guelph, Ontario in 1970 and 1971 with the objective of determining the relationships among whole-plant dry matter (DM) yield, whole-plant moisture content, and grain moisture content of corn (Zea mays L.) during the later part of the growing season. Each experiment involved eight commercial hybrids representative of the range in maturity, endosperm type, lodging resistance, and grain yield potential of corn hybrids grown commercially in central Ontario. The hybrids were sampled at weekly intervals over an 8-wk period beginning approximately 1 September; the sampled plants were divided into their leaf, stalk, husk, ear and grain components and oven-dried. Fresh and dry weights were used to calculate dry matter (DM) yields and "at harvest" moisture contents of the various components, and of the entire plant. Averaged across the eight hybrids, maximum DM yield was attained at whole-plant moisture content of 66–70%, and a grain moisture content of 45–50%. Among hybrids, 66% whole-plant moisture corresponded to a range in grain moisture content from 41 to 47%. Two additional experiments were grown also at Elora in 1970 and 1971 to evaluate the effects of harvest date on the DM yield and in vitro digestibility of corn plants and their component plant parts. Each experiment involved four representative commercial hybrids which were sampled at four equal time intervals during the month of September, and divided into grain, cob, husks (including shank) and stover (including leaves, leaf sheaths, stalks and tassels) for dry weight and in vitro digestibility measurement. Whole-plant DM digestibility was essentially constant over a range of whole-plant moisture from 76 to 56% in 1970, and from 76 to 64% in 1971. The consistency of whole-plant digestibility was the result of compensating changes in component yield and digestibility. A decrease in the digestibility of the stover, husks and cob with delayed harvest was compensated for by an increase in the proportion of grain in the whole-plant yield.

scholarly journals The “embryo in planta – callus in vitro” system: cytophysiological aspects (by wheat example)

2020 ◽  
Author(s):  
N. N. Kruglova ◽  
O. A. Seldimirova
Keyword(s):  
Plant Regeneration ◽  
Immature Embryos ◽  
In Planta ◽  
In Vitro System

Plant regeneration from calli in vitro is an integral part of a number of biotechnologies. Immature embryos (IE) are particularly promising as explants for obtaining morphogenic calli (MC) in cereals.

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

Digestibility and chemical composition of fractions of lucerne during spring and summer

1970 ◽  
Vol 75 (2) ◽  
pp. 213-222 ◽  
Author(s):  
K. R. Christian ◽  
D. B. Jones ◽  
M. Freer
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Dry Weight ◽  
Chemical Components ◽  
Shoot Height ◽  
Time Of Year ◽  
Highly Correlated ◽  
Leaf Composition

SUMMARYBulk harvests of primary growth were made at intervals during the spring and summer of 2 consecutive years from an ungrazed area of lucerne (Medicago sativa) and the digestibility by sheep was measured. At the same times, shoots of known length representative of the sward were collected, divided into 7·5 cm lengths, and separated into leaf and stem for the determination of ash, nitrogen, cellulose, and in vitro organic matter digestibility.Digestibility and leaf: stem ratio of the bulk harvests decreased and dry matter increased as the season advanced. Leaf composition changed little, but stem increased in lignin and decreased in ash and in vitro digestibility. In vitro digestibility and leaf: stem ratio were highly correlated with sheep digestibility.Chemical composition of shoots of known heights was similar to that of the bulk harvests. Leaf weights/shoot tended to increase linearly with height of shoot, whereas stem increased nearly as the square of the height. Dry matter of leaf and stem were inversely related to leaf:stem ratio. Stem digestibility decreased with leaf:stem ratio and with increase in shoot height.Chemical components of shoot fractions were associated with each other and were largely dependent on the distance from the top of the shoot at which they had been taken, irrespective of shoot height or time of year. Bottom stems had lower ash, nitrogen and digestibility but higher cellulose, dry weight and dry matter than top stems, whose composition approached that of leaves. Leaf fractions showed little consistent trend with position on the shoot.Some of the difficulties in studying changes in plant composition under field conditions are discussed.

PREDICTED FORAGE VALUE OF WHOLE PLANT CEREALS

1975 ◽  
Vol 55 (4) ◽  
pp. 975-986 ◽  
Author(s):  
L. J. FISHER ◽  
D. B. FOWLER
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Dry Weight ◽  
Neutral Detergent Fiber ◽  
Dry Matter Yield ◽  
Acid Detergent Fiber ◽  
Whole Plant ◽  
Digestible Organic Matter ◽  
Forage Value

Dry matter yield, percent dry weight, in vitro digestible dry matter, in vitro digestible organic matter, neutral detergent fiber, acid detergent fiber, ash and hemicellulose contents were determined for spring- and fall-sown common wheat, barley, rye and triticale and spring-sown durum wheat and oats for the period from late boot to maturity. Differences among cultivars and stages of maturity were significant for all parameters. These differences were accompanied by stage of maturity interactions. Consideration of the interrelationships among these parameters revealed that level of in vitro digestible dry matter was reflected in measures of acid detergent fiber and ash or percent dry weight. Further analyses indicated that differences in digestibility due to stage of maturity were primarily reflected by changes in ash or percent dry weight, while differences in digestibility among cultivars were mainly attributable to differences in acid detergent fiber.

Sign in / Sign up

Export Citation Format

Share Document