scholarly journals A new technique for nonsurgical embryo recovery in superovulated ewes treated with estradiol and oxytocin

2011 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
O. I. Azawi
Keyword(s):  
Ovarian Response ◽  
Practical Method ◽  
Laparoscopic Technique ◽  
Corpora Lutea ◽  
Cervical Canal ◽  
Transfer Programs ◽  
Embryo Recovery ◽  
Significant Difference ◽  
Group 2 ◽  
A New Technique

The development of an efficient and practical method for transcervical collection of embryos in Awassi ewes to the improvement of embryo recovery was investigated. Twenty-four non-pregnant Awassi ewes of 3-4 years of age were randomly allocated into two groups (n = 12). Each ewe was treated with a progesterone impregnated intra-vaginal sponge for 12 days. The following superovulation treatment was used: ewes received 1200 IU of eCG once as an intramuscular injection 48 h prior to sponge withdrawal. Ovarian response was assessed by determining the number of corpora lutea by laparoscopy at day 6 after mating. Ewes of group 1 (n = 12) their embryos were recovered using the semi-laparoscopic technique. Ewes of group 2 (n = 12) their embryos were recovered using the transcervical technique. All ewes of this group on the evening of day 5 post inseminations, received an i.m. injection of estradiol 17β 2 mg, 12h later, i.v. injection of 10 IU of oxytocin. A bovine teat dilator introduced into the cervical canal and gradually dilated in order to dilate and open the cervical canal with a help of screw provided with the instrument. Results of the present study showed that there was no significant difference in the number of recovered embryos in superovulated ewes when animals treated with estradiol and oxytocin with transcervical technique and semi-laparoscopic technique. It was possible to pass the catheter through the cervix with the aid of bovine teat dilator enhanced by estradiol and oxytocin treatment. In conclusion, the technique for transcervical collection of the embryo from ewes, as described in the present study, may give new encouragement to embryo transfer programs in this species.

scholarly journals Laparoscopic intrauterine insemination of Awassi ewes superovulated with equine chorionic gonadotropin

2010 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
O. I. Azawi, and M. K. M. A. Al-Mola
Keyword(s):  
Chorionic Gonadotropin ◽  
Intrauterine Insemination ◽  
Ovarian Response ◽  
Fertilization Rate ◽  
Distal Portion ◽  
Laparoscopic Technique ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
Equine Chorionic Gonadotropin ◽  
Laparoscopic Techniques

The present study was undertaken to investigate the contribution of laparoscopic insemination to the improvement of fertilization and embryo recovery. The experiment was conducted in the breeding season. Twelve nonpregnant and cycling Awassi ewes of 3-4 years of age were randomly allocated in equal number (n = 6) to two groups. Each ewe was treated with progesterone impregnated intravaginal sponge for 12 days. All ewes were superovulated with eCG (equine chorionic gonadotropin) 1200 IU once by intramuscular injection 48 h prior to sponge removal. Ewes of group 1 were mated naturally at least two times with Awassi rams of proven fertility. Ewes of group 2 had intrauterine insemination and were conducted 44-46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of semen in the distal portion of each uterine horn. Ovarian response was assessed by determining the number of corpora lutea by laparoscopy on day 6 after mating. Embryo recovery was performed by semi-laparoscopic and by flushing of both uterine horns. Results of the present study revealed a high number of unfertilized ova (P<0.05) was observed in ewes when naturally inseminated than ewes inseminated intrauterine using the laparoscopic technique. The higher rate of embryo recovery (P<0.05) was achieved when ewes inseminated via intrauterine (4.66 ± 0.66) than ewes naturally mated (2.16 ± 0.74). The fertilization rate in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 79.93% and 40.07%, respectively. Fertilization failure in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 7.37% and 49.35%, respectively. It could be concluded that the use of eCG to induce superovulation in Awassi ewes combined with laparoscopic intrauterine insemination can increase the fertilization rate.

295 SUPEROVULATORY RESPONSE IN COWS FOLLOWING SYNCHRONIZATION OF FOLLICLE WAVE EMERGENCE WITH ESTRADIOL AT DIFFERENT STAGES OF THE ESTROUS CYCLE

2008 ◽  
Vol 20 (1) ◽  
pp. 227
Author(s):  
A. Garcia Guerra ◽  
G. A. Bó ◽  
J. Villarreal ◽  
G. M. Brogliatti
Keyword(s):  
Estrous Cycle ◽  
Buenos Aires ◽  
Animal Health ◽  
Ovarian Response ◽  
Buenos Aires Province ◽  
Transfer Program ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Significant Difference ◽  
Group 2

Ovarian asynchrony and variability in response to superstimulation remain the most limiting factors in any embryo transfer program (Armstrong D 1993 Theriogenology 39, 7–24). Ovarian response can be increased and less variable if superstimulatory treatment is started at the time of follicular wave emergence (Bö GA et al. 1995 Theriogenology 43, 31–40). A combination of progesterone (P4) and estradiol have been used to synchronize follicular wave for superstimulation. A retrospective analysis was done to compare the ovarian response, superovulatory response and embryo production of cows in Argentina that received progesterone and estradol prior to superstimulation at different stages of the estrous cycle. This research was carried out using different breed of donors (n = 584, 88% Angus) during the last 4 years in Buenos Aires province, Argentina. Heat detection was performed twice a day. At random stages of the estrous cycle, donors received an intravaginal progesterone device (DIB; Syntex, Buenos Aires, Argentina), 2 mg of estradiol benzoate and 50 mg of progesterone (Syntex, Buenos Aires, Argentina) IM on the same day. On day 4 after DIB insertion, superestimulatory treatment was initiated on a decreasing dose regimen of FSH (Pluset; Callier, Spain, or Folltropin, Bioniche Animal Health Inc., Belleville, Ontario, Canada) as IM injections every 12 h over 4 d. On day 6, DIBs were removed, and cows received two doses of 2 mL of cloprostenol 12 h apart. At heat detection, all donors received a dose of 2 mL of GnRH (Dalmarelin; Fatro Von Franken, Buenos Aires, Argentina) by IM injection and were inseminated 12 and 24 h later. Seven days later, embryo collection was performed and ovarian response was evaluated as number of CL + unovulated follicles by transrectal ultrasound using a 7.5-MHz transducer (Pie Medical, Maastricht, the Netherlands). Ova/embryos were evaluated and classified according to the IETS manual. Donors were assigned to receive DIB and estradiol during the following stages of the cycle: group 1: between days 4 and 7 post-estrus (dominant follicle period), group 2: between days 8 and 12 post-estrus (emergence of the second follicular wave), and group 3: between days 13 and 21 post-estrus (dominant follicle of the second wave). Kruskal-Wallis test was used to compare variables among groups, and results are shown in Table 1. Ovarian response as CL + unovulated follicles and number of ovulations were significantly different among groups (P < 0.05). However, there was no significant difference in the number of fertilized ova or transferable embryos. Nevertheless, numeric differences that show that group 2 (started between days 8 and 12 post-estrus) was always superior for all variables. In conclusion, data suggest that estradiol may be more effective in synchronizing follicle wave emergence for superstimulation during the mid-part of the estrous cycle. Table 1. Superovulatory response in cows in which follicle wave emergence was synchronized with estradiol at different stages of the estrous cycle (mean ± SD) Research supported by Centro Genetico Bovino Eolia S.A.

Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotrophin and follicle stimulating hormone

1991 ◽  
Vol 3 (5) ◽  
pp. 551 ◽  
Author(s):  
JP Ryan ◽  
JR Hunton ◽  
WM Maxwell
Keyword(s):  
Follicle Stimulating Hormone ◽  
Ovarian Response ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
Designed Experiment ◽  
The Mean ◽  
Mean Concentration ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

In a factorially designed experiment (N = 321), 0, 800 or 1600 I.U. pregnant mare serum gonadotrophin (PMSG) were administered in combination with 0, 12 or 18 mg follicle stimulating hormone (FSH-P) to superovulate Merino ewes in autumn and spring. A moderate dose of PMSG (800 I.U.) in conjunction with 12 or 18 mg FSH-P increased the ovulation rate above that observed when FSH-P was used alone. This was accomplished by (i) increasing the proportion of ewes that exhibited a superovulatory response (greater than 3 corpora lutea (CL) or persistent large follicles (LF): 69/70 (99%) v. 55/74 (74%), P less than 0.001), and (ii) in those ewes that exhibited a superovulatory response, by an additive effect of exogenous gonadotrophin (14.8 +/- 0.9 CL (69) v. 11.3 +/- 0.9 CL (55), P less than 0.01) without increasing the incidence of LF. The use of 1600 I.U. PMSG in conjunction with 12 or 18 mg FSH-P was characterized by an increase in the number of LF and, in comparison with 800 I.U. PMSG, a reduction in ovulation rate. Season had no effect on the numbers of CL, but total ovarian response (CL + LF) was higher in autumn than in spring (P less than 0.01), because of a greater incidence of LF (P less than 0.001). The proportion of ewes with regressed CL was higher in autumn than in spring (53/143 (37%) v. 32/156 (21%), P less than 0.01), and increased with increased dose of gonadotrophin. Furthermore, a nutritional component to the incidence of ewes with regressed CL was suggested by the observation that the mean concentration of plasma glucose was higher for ewes with normal CL than for ewes with regressed CL (P less than 0.05). Rates of ova or embryo recovery, fertilization and embryo development generally declined with an increase in the incidence of LF as a result of increases in the dose of gonadotrophin and season of administration.

224 EFFECT OF LENGTHENING THE SUPERSTIMULATORY TREATMENT PROTOCOL ON THE OVARIAN RESPONSE AND NUMBERS OF TRANSFERABLE EMBRYOS IN BEEF COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 224
Author(s):  
A. Garcia Guerra ◽  
A. Tribulo ◽  
J. Yapura ◽  
J. Singh ◽  
R. J. Mapletoft
Keyword(s):  
Total Dose ◽  
Collection Efficiency ◽  
Animal Health ◽  
Treatment Protocol ◽  
Prostaglandin F2α ◽  
Ovarian Response ◽  
Beef Cows ◽  
Control Group ◽  
Corpora Lutea ◽  
Significant Difference

The present study was designed to test the hypothesis that an increase in the length of exposure of ovulatory follicles to progesterone and FSH during ovarian superstimulation will increase the number of ovulations and viable embryos in cattle. Twenty-four beef cows were initially subjected to follicle ablation at random stages of the oestrous cycle to determine the number of follicles at wave emergence; cows with comparable numbers of follicles were randomly allocated to groups by replicate. A single dose of prostaglandin F2α (PGF; Estroplan, Vétoquinol, QC, Canada) was given IM 7 to 9 days after follicle ablation and daily ultrasound examinations were performed to detect ovulation. Follicular ablation of all follicles ≥5 mm was done 5 to 8 days after ovulation and a progesterone-releasing intravaginal device (PRID, Vétoquinol) was inserted. The Control group (n = 12) was treated with 4 days of FSH and cows in the Long group (n = 12) were given 7 days of FSH treatment. The FSH treatments were initiated 1.5 days after ablation (Day 0) with a total dose of 400 mg of NIH-FSH-P1 (Folltropin-V; Bioniche Animal Health, Belleville ON, Canada). The total dose of FSH was distributed equally over 8 (Control) or 14 (Long) IM injections at 12-h intervals. Prostaglandin F2α was administered twice (at 12-h intervals) on Day 2 (Control) or Day 5 (Long) and PRID were removed 12 h after the last PGF. Both groups received 25 mg of porcine LH (pLH) IM (Lutropin-V; Bioniche Animal Health) 24 h after PRID removal and AI with frozen–thawed semen of proven fertility was done 12 and 24 h later. A third AI was done 12 h later in cows with 2 or more follicles ≥9 mm (12/12 and 9/12 in Control and Long groups, respectively; P = 0.22). All animals were subjected to transrectal ultrasonography every other day and at the time of ova or embryo collection. Ova or embryos were collected nonsurgically 7 days after the pLH injection and evaluated following IETS guidelines. Embryos were defined as transferable (Grades 1, 2 and 3) and freezable (Grades 1 and 2). Procedure Genmod was used to compare variables between treatments and results are presented as means ± SEM (Table 1). There was no significant difference in the total numbers of ova/embryos recovered, but there were more ovulations, corpora lutea (CL), fertilized ova and transferable and freezable embryos in the Long group (P < 0.05). Collection efficiency (number of ova/embryos over the number of CL) was lower in the Long group (P < 0.05). In summary, lengthening of the superstimulatory treatment resulted in an increased number of ovulations without a decrease in oocyte or embryo competence. Data suggest that the traditional 4-day superstimulatory treatment protocol provides inadequate time to maximize ovulatory response. Table 1.Superovulatory response (mean ± SEM) of cows subjected to a conventional 4-day (Control) or a 7-day (Long) treatment protocol

196 Fertilization Rate in Superovulated Criolla Goats Following Artificial Insemination or Natural Mating

2018 ◽  
Vol 30 (1) ◽  
pp. 238
Author(s):  
M. I. Cueto ◽  
J. Fernandez ◽  
M. M. Bruno-Galarraga ◽  
F. Pereyra-Bonnet ◽  
A. Gibbons
Keyword(s):  
Statistical Significance ◽  
Genetic Material ◽  
Fertilization Rate ◽  
Corpora Lutea ◽  
Embryo Production ◽  
Transfer Programs ◽  
Embryo Recovery ◽  
Frozen Semen ◽  
Natural Mating ◽  
Material Studies

The Criolla breed is a local genetic resource, distributed in Patagonia, Argentina, whose primary production is meat. In the Criolla goat, efforts were made to locate productively superior males and to conserve their genetic material. Studies were carried on the feasibility of obtaining superior offspring through the implementation of embryo transfer programs. We assessed the fertilization rate and embryo production following AI with frozen semen or natural mating in Criolla goats subjected to a superovulation program. During the breeding season (May, 41° S), 26 Criolla goats were treated for oestrus with sponges (60 mg of medroxyprogesterone acetate, Progespon®, Syntex, Buenos Aires, Argentina) placed for 13 days. Goat donors were superovulated with a total of 80 mg of porcine (p)FSH (Folltropin V®, Bioniche, Ontario, Canada) every 12 h in 6 decreasing doses (18, 18, 14, 14, 8, and 8 mg) during the last 3 days of progestagen treatment. A dose of 125 μg of cloprostenol (Estrumate®, Schering-Plough, Quebec, Canada) was given in conjunction with the first dose of pFSH. Oestrus detection was performed every 12 h, starting at 24 h after sponge removal. Females were considered to be in oestrus if they passively accepted buck mounting. Goats were randomly assigned to the following treatments: (1) natural mating (NM, n = 12): donors detected in oestrus were individually mated with one proven fertile buck at oestrus and 12 h post-oestrus; does were remove from the male in between (Conventionally accepted treatment); (2) AI (n = 14): donors detected in oestrus were inseminated 12 ± 2 h after the onset of oestrus by laparoscopy with frozen-thawed semen (200 × 106 spermatozoa) from the same fertile buck. Embryo recovery was done by surgical prepubic laparotomy at Day 8 after sponge removal. Superovulation response was estimated by counting the number of corpora lutea (CL). Analysis of variance was used to compare fertilization rate (total number of embryos recovered for each animal, expressed as a proportion of the total number of embryos/oocytes recovered) and embryo production between treatments. Results were expressed as mean ± SEM. Statistical significance was accepted at P < 0.05. A total of 92.3% goats were recorded in oestrus (24/26) between 24 and 48 h after sponge removal (10 and 14 goats for NM and AI, respectively). An average of 16.6 ± 2.0 CL (range: 2–32) was observed in response to superovulation treatment. The recovery rate of embryos/oocytes was 60.0 ± 6.9%. No statistical difference was observed in the fertilization rate (52.1 ± 12.1 and 68.6 ± 12.1% for AI and NM, respectively) or the number of total (6.8 ± 2.0 and 4.8 ± 2.4 for AI and NM, respectively) and transferable embryos (5.6 ± 1.4 and 4.0 ± 1.7 for AI and NM, respectively) between treatments (P > 0.05). In conclusion, fertilization rates did not differ following laparoscopic insemination with frozen semen compared to natural mating in superovulated Criolla goats.

Successful embryo transfer following artificial insemination of superovulated fallow deer (Dama dama)

1994 ◽  
Vol 6 (2) ◽  
pp. 181 ◽  
Author(s):  
HN Jabbour ◽  
VS Marshall ◽  
CM Argo ◽  
J Hooton ◽  
AS Loudon
Keyword(s):  
Ovarian Response ◽  
Fallow Deer ◽  
Corpora Lutea ◽  
Dama Dama ◽  
Cell Stage ◽  
Embryo Recovery ◽  
Dama Mesopotamica ◽  
Morula Stage ◽  
The Mean ◽  
Follicular Response

Thirty-four European fallow deer (Dama dama dama) were randomly allocated into embryo donor (n = 12) or embryo recipient (n = 22) groups. All does were treated with controlled internal drug release (CIDR) devices for 14 days. Animals in the embryo donor group were further treated with 200 I.U. pregnant mare serum gonadotrophin (PMSG) and 0.5 units ovine follicle-stimulating hormone (FSH). PMSG was administered 72 h before withdrawal of CIDR devices and FSH was given in eight 0.063 unit injections at 12-hourly intervals starting at the time of PMSG administration. All embryo donor animals were inseminated, by laparoscopy in both uterine horns, 36 h after withdrawal of CIDR devices with 25 x 10(6) fresh spermatozoa collected from Mesopotamian fallow deer (Dama dama mesopotamica). Embryos were recovered by laparotomy on Day 3 (n = 6) or Day 6 (n = 6) after withdrawal of CIDR devices and the ovarian response was determined. In total, 22 embryos were transferred into the oviduct (2-4-cell stage, n = 14) or uterine horn (morula stage, n = 8) on Day 3 or Day 6 after withdrawal of CIDR devices respectively. The overall means (+/- s.e.m.) of total follicular response and corpora lutea were 24.2 +/- 3.5 and 14.1 +/- 3.6 respectively. The mean number of large unruptured follicles was higher on Day 6 than on Day 3 (13.5 +/- 2.9 v. 6.7 +/- 1.3, P < 0.05). The overall embryo recovery rate was 45.8%. There was no difference in pregnancy rate following transfer of embryos on either Day 3 or Day 6 (7/14 v. 5/8 respectively).(ABSTRACT TRUNCATED AT 250 WORDS)

154 EVALUATION OF EMBRYO RECOVERY RATE AND EMBRYO TRANSFER IN ANGORA GOATS

2015 ◽  
Vol 27 (1) ◽  
pp. 168
Author(s):  
K. Karakas ◽  
H. Alkan ◽  
G. Onur ◽  
D. Ozen ◽  
M. Kaymaz ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Recovery Rate ◽  
Pregnancy Rates ◽  
Corpora Lutea ◽  
Embryo Survival ◽  
Embryo Recovery ◽  
Angora Goats ◽  
Group 2 ◽  
Group 1

The aims of this study were to compare the embryo recovery rate in Angora goats based on application timing; at the beginning (September – October; Group 1) and end (December; Group 2) of the breeding season and to evaluate the viability and survivability of fresh or vitrified-thawed embryos when transferred. For this purpose, nine Angora goats were used as donors and thirthy Angora goats were used as recipients. Donor goats were synchronized and superovulated with traditional protocol and were mated with fertile bucks. At the 156th hour of the mating, embryos were collected surgically and evaluated under a stereo microscope. In group 1, 103 embryos and in group 2, 63 embryos were collected from nine goats. Fresh or vitrified-thawed embryos were transferred surgically to synchronized recipients. In Group 1 fresh/thawed embryos were transferred to 15/15 goats and in group 2, fresh/thawed embryos were transferred to 8/8 goats, respectively. Each recipient received 1 or 2 embryos ipsilateral to the ovary containing one or more corpora lutea. On day 30 of the transfer, goats were examined by transrectal ultrasonography, pregnancy rates of fresh/thawed embryos were 66.6%/26.6% for group 1 and 62.5%/62.5% for group 2. On day 100 of the transfer, goats were examined again by ultrasonography, and pregnancy rates were 46.6%/0% for group 1 and 37.5%/0% for group 2, respectively. After about 50 days, goats were kidded. In group 1, 3 twins and 4 single kids were born; in group 2, 2 twins were born. The total number of collected embryos and pregnancy rates among the groups were analysed using SPSS® (version 14.01, Chicago, IL, USA) and for all comparisons, differences were considered with a minimum of 5% significance level. After statistical analyses, the numbers of collected embryos at the beginning and at the end of the breeding season were compared. There was no difference in freezable/transferable embryo quality. As a result, embryos could be collected after superovulation protocols in Angora goats both at beginning and end of the breeding season, however there might be a decrease in numbers of collected embryos and the reasons for this might not be only the seasonal factors but also the environment, care, nutrition and previous superovulation protocols. The pregnancy rate following transfer of fresh embryos was satisfactory but not all does confirmed pregnant kidded; hence, reducing the number of recipients kidding. The pregnancy rate following transfer of vitrified-thawed embryos was generally low and unsatisfactory. Further research is warrented in improving the cryopreservation techniques and thus the embryo survival rate of Angora goat embryos. This study was financed with the University of Ankara Grant.

107 EARLY EMBRYONIC SURVIVAL IN THE PIG IS HIGHER THAN CONVENTIONALLY REPORTED

2009 ◽  
Vol 21 (1) ◽  
pp. 153 ◽  
Author(s):  
E. Østrup ◽  
M. Vejlsted ◽  
M. B. Nielsen ◽  
M. T. Madsen ◽  
P. Maddox-Hyttel
Keyword(s):  
Recovery Rate ◽  
Primitive Streak ◽  
Embryonic Mortality ◽  
Corpora Lutea ◽  
Gestational Length ◽  
Crown Rump Length ◽  
Embryo Recovery ◽  
Somite Stage ◽  
Group 2

It is generally accepted that 30% of the embryos in a porcine litter die within the first 40 days of pregnancy (Pope WP and First NL 1985 Theriogenology 23, 91–105). The aim of the study was to investigate the dynamics of embryonic mortality from the 2nd to the 7th week of pregnancy in a homogeneous pig population in order to test whether this dogma holds true. A total of 141 pregnant Danish Landrace × Yorkshire gilts were divided into three groups dependent on gestational length: Group 1 (Days 9 to 24 post insemination (p.i.)): At Days 9 to 18 p.i., embryos were collected by flushing the uterine horns with PBS containing 1% serum. At Days 19 to 24, embryos were identified in situ by opening of the horns along the anti-mesometrial side. All embryos were staged according to the morphological appearance of embryo proper. Pre-somite stage embryos were categorized as either: Hatched blastocysts, pre-streak 1, pre-streak 2, primitive streak, or neural groove stage embryos (Vejlsted M et al. 2006 Mol. Reprod. Dev. 73, 709–718). Somite stage embryos were staged according to the number of somites. All embryos in Group 2 (Days 24.5 to 33 p.i.) and Group 3 (Days 40.5 to 47 p.i.) were identified in situ by opening the uterine horns as described above. The localization in the uterus and the Crown Rump Length (CRL) was recorded for all embryos in these groups. The average embryo recovery rate, (i.e. the ratio between the numbers of recovered embryos and the CL numbers) was 82%. Moreover, there were no significant differences between the groups with respect to the embryo recovery rate, signaling the absence of continued embryonic mortality. No significant correlations were obtained between the location of the embryos in the uterus and the CRL (only measured for Groups 2 and 3). Our data indicate that (1) the level of embryonic mortality was less than 10 to 15% and (2) there was no continued embryonic mortality occurring between Days 9 to 47 p.i. This is in great contrast to previous reports. Furthermore, there is no evidence that the location in the uterine horn has any influence on the embryonic development. Table 1.The average numbers of corpora lutea (CL), embryos, and the embryo recovery rates in gilts at different time points after insemination The Danish Research Council for Technology and Production Sciences.

scholarly journals Absence of mutagenic effect of Mikania glomerata hydroalcoholic extract on adult wistar rats in vivo

2006 ◽  
Vol 49 (4) ◽  
pp. 599-604 ◽  
Author(s):  
Rita de Cássia da Silveira e Sá ◽  
Magda Narciso Leite ◽  
Vera Maria Peters ◽  
Martha de Oliveira Guerra ◽  
Reinaldo Nóbrega de Almeida
Keyword(s):  
Wistar Rats ◽  
Mutagenic Effect ◽  
Corpora Lutea ◽  
Hydroalcoholic Extract ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Group 2 ◽  
Mikania Glomerata ◽  
Group 1

This work makes an assessment of the dominant lethality of Mikania glomerata in male Wistar rats. Adult male received 1 mL of M. glomerata hydroalcoholic extract at a dose level of 3.3 g/kg body weight for 52 days and were mated with untreated females for seven weeks (group 1) or one week prior to the beginning of treatment and on the week following the end of treatment (group 2). The parameters analyzed were: number of implanted embryos, resorptions and corpora lutea; mating, gestation, preimplantation loss, implantation and resorption indexes (group 1); number of offspring and weaning animals (group 2). The administration of M. glomerata did not show any impairment of fertility and no significant difference in the parameters analyzed, suggesting an absence of mutagenic effect on Wistar rats.

scholarly journals Capsulorhexis Under Continuous Fluid Pressure in Intumescent Cataracts:  A New Technique

2021 ◽  
Author(s):  
Fikret UÇAR
Keyword(s):  
Anterior Chamber ◽  
Fluid Pressure ◽  
Cell Loss ◽  
Anterior Capsule ◽  
Significant Difference ◽  
Radial Tear ◽  
Group 2 ◽  
A New Technique ◽  
Efficiency And Reliability ◽  
Group 1

Abstract Purpose: To evaluate the effectiveness of the anterior capsulorhexis technique under continuous fluid pressure with anterior chamber maintainer in intumescent cataracts.Methods: Scheduled for phacoemulsification due to intumescent cataracts, 84 eyes of 84 patients were included in the study. An anterior chamber maintainer, which provided continuous fluid pressure in the anterior chamber, was placed in the patients of Group 1 (n = 42). Capsulorhexis without viscoelastic was then applied under continuous fluid pressure. In Group 2 (n = 42) patients, capsulorhexis was performed by utilizing an ophthalmic viscoelastic device (OVD) to the anterior chamber. The efficiency and reliability of our capsulorhexis method were evaluated by comparing the intraoperative and postoperative complications that occurred in such cases.Results: Radial tear did not occur in any of the cases in the capsulorhexis stage in Group 1; however, 6 Group 2 patients were observed to have radial tears(p<0.05). Progression of the anterior capsule, which did not turn into a radial tear, to the periphery was observed in 2 patients in Group 1, and 8 patients in Group 2(p<0.05). When liquefied cortical material egress dynamics were evaluated; the controlled fluid output was observed in 41 patients (97.61%) in Group 1, and 29 patients (69.4%) in Group 2 (p<0.001). There was no statistically significant difference revealed between the two groups in the endothelial cell loss (p> 0.05).Conclusion: The technique we used provides a controlled and safe capsulorhexis in intumescent cataracts, reduces surgical complications, and does not require any additional cost.

Sign in / Sign up

Export Citation Format

Share Document