107 EARLY EMBRYONIC SURVIVAL IN THE PIG IS HIGHER THAN CONVENTIONALLY REPORTED

2009 ◽  
Vol 21 (1) ◽  
pp. 153 ◽  
Author(s):  
E. Østrup ◽  
M. Vejlsted ◽  
M. B. Nielsen ◽  
M. T. Madsen ◽  
P. Maddox-Hyttel
Keyword(s):  
Recovery Rate ◽  
Primitive Streak ◽  
Embryonic Mortality ◽  
Corpora Lutea ◽  
Gestational Length ◽  
Crown Rump Length ◽  
Embryo Recovery ◽  
Somite Stage ◽  
Group 2

It is generally accepted that 30% of the embryos in a porcine litter die within the first 40 days of pregnancy (Pope WP and First NL 1985 Theriogenology 23, 91–105). The aim of the study was to investigate the dynamics of embryonic mortality from the 2nd to the 7th week of pregnancy in a homogeneous pig population in order to test whether this dogma holds true. A total of 141 pregnant Danish Landrace × Yorkshire gilts were divided into three groups dependent on gestational length: Group 1 (Days 9 to 24 post insemination (p.i.)): At Days 9 to 18 p.i., embryos were collected by flushing the uterine horns with PBS containing 1% serum. At Days 19 to 24, embryos were identified in situ by opening of the horns along the anti-mesometrial side. All embryos were staged according to the morphological appearance of embryo proper. Pre-somite stage embryos were categorized as either: Hatched blastocysts, pre-streak 1, pre-streak 2, primitive streak, or neural groove stage embryos (Vejlsted M et al. 2006 Mol. Reprod. Dev. 73, 709–718). Somite stage embryos were staged according to the number of somites. All embryos in Group 2 (Days 24.5 to 33 p.i.) and Group 3 (Days 40.5 to 47 p.i.) were identified in situ by opening the uterine horns as described above. The localization in the uterus and the Crown Rump Length (CRL) was recorded for all embryos in these groups. The average embryo recovery rate, (i.e. the ratio between the numbers of recovered embryos and the CL numbers) was 82%. Moreover, there were no significant differences between the groups with respect to the embryo recovery rate, signaling the absence of continued embryonic mortality. No significant correlations were obtained between the location of the embryos in the uterus and the CRL (only measured for Groups 2 and 3). Our data indicate that (1) the level of embryonic mortality was less than 10 to 15% and (2) there was no continued embryonic mortality occurring between Days 9 to 47 p.i. This is in great contrast to previous reports. Furthermore, there is no evidence that the location in the uterine horn has any influence on the embryonic development. Table 1.The average numbers of corpora lutea (CL), embryos, and the embryo recovery rates in gilts at different time points after insemination The Danish Research Council for Technology and Production Sciences.

154 EVALUATION OF EMBRYO RECOVERY RATE AND EMBRYO TRANSFER IN ANGORA GOATS

2015 ◽  
Vol 27 (1) ◽  
pp. 168
Author(s):  
K. Karakas ◽  
H. Alkan ◽  
G. Onur ◽  
D. Ozen ◽  
M. Kaymaz ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Recovery Rate ◽  
Pregnancy Rates ◽  
Corpora Lutea ◽  
Embryo Survival ◽  
Embryo Recovery ◽  
Angora Goats ◽  
Group 2 ◽  
Group 1

The aims of this study were to compare the embryo recovery rate in Angora goats based on application timing; at the beginning (September – October; Group 1) and end (December; Group 2) of the breeding season and to evaluate the viability and survivability of fresh or vitrified-thawed embryos when transferred. For this purpose, nine Angora goats were used as donors and thirthy Angora goats were used as recipients. Donor goats were synchronized and superovulated with traditional protocol and were mated with fertile bucks. At the 156th hour of the mating, embryos were collected surgically and evaluated under a stereo microscope. In group 1, 103 embryos and in group 2, 63 embryos were collected from nine goats. Fresh or vitrified-thawed embryos were transferred surgically to synchronized recipients. In Group 1 fresh/thawed embryos were transferred to 15/15 goats and in group 2, fresh/thawed embryos were transferred to 8/8 goats, respectively. Each recipient received 1 or 2 embryos ipsilateral to the ovary containing one or more corpora lutea. On day 30 of the transfer, goats were examined by transrectal ultrasonography, pregnancy rates of fresh/thawed embryos were 66.6%/26.6% for group 1 and 62.5%/62.5% for group 2. On day 100 of the transfer, goats were examined again by ultrasonography, and pregnancy rates were 46.6%/0% for group 1 and 37.5%/0% for group 2, respectively. After about 50 days, goats were kidded. In group 1, 3 twins and 4 single kids were born; in group 2, 2 twins were born. The total number of collected embryos and pregnancy rates among the groups were analysed using SPSS® (version 14.01, Chicago, IL, USA) and for all comparisons, differences were considered with a minimum of 5% significance level. After statistical analyses, the numbers of collected embryos at the beginning and at the end of the breeding season were compared. There was no difference in freezable/transferable embryo quality. As a result, embryos could be collected after superovulation protocols in Angora goats both at beginning and end of the breeding season, however there might be a decrease in numbers of collected embryos and the reasons for this might not be only the seasonal factors but also the environment, care, nutrition and previous superovulation protocols. The pregnancy rate following transfer of fresh embryos was satisfactory but not all does confirmed pregnant kidded; hence, reducing the number of recipients kidding. The pregnancy rate following transfer of vitrified-thawed embryos was generally low and unsatisfactory. Further research is warrented in improving the cryopreservation techniques and thus the embryo survival rate of Angora goat embryos. This study was financed with the University of Ankara Grant.

The effect of stage of breeding season or pre-mating oestrogen and progestagen therapy on fertility in ewe lambs

1994 ◽  
Vol 59 (3) ◽  
pp. 429-434 ◽  
Author(s):  
N. F. G. Beck ◽  
M. C. G. Davies
Keyword(s):  
Breeding Season ◽  
Corpora Lutea ◽  
Oestrogen Therapy ◽  
Crown Rump Length ◽  
Ewe Lambs ◽  
Oestradiol Benzoate

AbstractAn investigation was conducted to determine the effect of mating at puberty or third oestrus or after pre-mating oestrogen and progestagen therapy, designed to mimic the changes that occur in plasma oestrogen and progesterone concentrations during the 1st, 2nd or 3rd oestrous cycles, on fertility in ewe lambs. Twenty-five ewe lambs were mated at puberty on mean date of 18 October and 21 were mated at third oestrus on 3 December. Of these, 28% and 81% of animals mated at puberty and third oestrus lambed, respectively (P < 0·05). A similar number of ewe lambs were mated within 24 h of ram introduction in the groups given one, two or three consecutive applications oestrogen and progestagen (25 μg oestradiol benzoate followed 48 h later by a progestagen pessary left in situ for days). Of the 43 corpora lutea, 21 were represented by viable embryos in the 37 animals holding to service after one application compared with 34 out of 46 corpora lutea in 40 animals given three applications (P < 0·05). Furthermore, embryo crown-rump length, embryo weight and amniotic sac width was greater (P < 0·05), on day of pregnancy, in animals given one compared with three applications of oestrogen and progestagen. These results demonstrate that fertility is improved in ewe lambs mated on third oestrus or after progestagen and oestrogen therapy designed to simulate three oestrous cycles.

108 COMPARATIVE EFFICIENCY OF GONADOTROPIN-RELEASING HORMONE AND LUTEINIZING HORMONE IN THE INDUCTION OF OVULATION IN SUPEROVULATED ALPACAS

2014 ◽  
Vol 26 (1) ◽  
pp. 168
Author(s):  
H. W. Vivanco-Mackie ◽  
M. D. Ponce Salazar ◽  
M. M. Gonzales ◽  
M. A. Tapia
Keyword(s):  
Gnrh Agonist ◽  
Recovery Rate ◽  
Pearson Correlation ◽  
Animal Health ◽  
Gonadotropin Releasing Hormone ◽  
Corpora Lutea ◽  
Peruvian Andes ◽  
Releasing Hormone ◽  
Induction Of Ovulation ◽  
Embryo Recovery

Alpacas are induced ovulators, responding to copulation and (or) exogenous application of ovulation-inducing hormones. The objective of this study was to determine the efficiency of the injection of a gonadotropin-releasing hormone (GnRH) agonist versus LH in the induction of ovulation and the presence and size of non-ovulated follicles at the time of embryo collection and its relationship to the yield of transferable embryos in superovulated alpacas. Twenty-one adult (3 to 7 years old) female alpacas under extensive grazing at 4300 m elevation in the Peruvian Andes that had been synchronized and treated for superovulation were induced to ovulate 6 days after the application of the superovulatory hormone (1000 IU of eCG, Folligon®, Intervet International BV, Boxmeer, the Netherlands) by mating with fertile males and injection immediately after copulation of either an IM dose of 0.0084 mg of buserelin acetate (Buserelina Zoovet®, Laboratorio Zoovet, Santa Fe, Argentina) to 10 alpacas (T1) or an IM dose of 5-mg Armour standard of LH (Lutropin®, Bioniche Animal Health, Belleville, ON, Canada) to 11 alpacas (T2). All alpacas had a second mating 12 h after the first mating and were subjected to ovarian inspection by ultrasonography and previous embryo collection by nonsurgical transcervical embryo flushing 6.5 days after the first mating. On average, the embryo recovery rate for T1 was 34.6% and there were 7.8 ± 3.99 corpora lutea (CL), 2.7 ± 4.08 collected embryos, and 3.6 ± 2.95 total, 0.5 ± 0.85 small (<6 mm), 1.8 ± 1.99 medium (≥6 but ≤14 mm), and 1.3 ± 2.11 large (≥15 mm) non-ovulated follicles. For T2, the embryo recovery rate was 59.4% and there were 6.73 ± 1.49 CL, 4.0 ± 2.57 collected embryos, and 0.64 ± 0.81 total, 0.0 ± 0.0 small, 0.36 ± 0.67 medium, and 0.27 ± 0.47 large non-ovulated follicles. The differences between treatments were nonsignificant (P > 0.05) for all the parameters; however, there was a clear tendency for a better recovery rate, more embryos collected, and lower number of non-ovulated follicles in T2. The Pearson correlation coefficient between the presence of large follicles in the ovaries at the time of embryo collection and the total number of embryos collected was negative (r = –0.26) and highly significant (P ≤ 0.001). The use of LH for ovulation induction of superovulated alpacas seems to be more advisable than the use of GnRH agonist; further comparisons with larger number of observations per treatment are recommended. This study was financed by the Peruvian Fund for Innovation, Science and Technology (FINCYT).

407 SUPERSTIMULATORY RESPONSE WITH DECREASING DOSES OF FOLLICLE-STIMULATING HORMONE v. A SINGLE DOSE OF FOLLICLE-STIMULATING HORMONE - EQUINE CHORIONIC GONADOTROPIN DURING BREEDING AND NONBREEDING SEASON IN SHEEP

2010 ◽  
Vol 22 (1) ◽  
pp. 360
Author(s):  
M. I. Cueto ◽  
F. Pereyra-Bonnet ◽  
P. Silvestre ◽  
A. E. Gibbons
Keyword(s):  
Single Dose ◽  
Breeding Season ◽  
Recovery Rate ◽  
Follicle Stimulating Hormone ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Nonbreeding Season ◽  
Embryo Recovery ◽  
The One ◽  
Stimulating Hormone

The aim of the study was to assess possible variations in superovulatory yields due to different FSH treatments at 2 times of the year. Superovulation and embryo recovery were performed during the breeding (n = 63) andnonbreeding (n = 46) seasons in Merino ewes located at 41°S latitude. Animals were kept under the same conditions, housed outdoors in a sheltered and covered pen, and were fed a liveweight maintenance ration. All animals received 60-mg medroxyprogesterone acetate intravaginal sponges (Progespon®, Syntex, Buenos Aires, Argentina) for 14 days. Ewes were then randomly assigned to 2 different superovulatory treatments: classic (n = 74) and one shot (n = 35) in both seasons. Classic superovulatory treatment consisted of 7 decreasing doses (2 × 48 mg, 2 × 24 mg, 2 × 20 mg, and1 × 16 mg NIH-FSH-P1)ofFSH (Folltropin®-V, Bioniche, Belleville, Ontario, Canada), administered twice daily from 48 h before to 24 h after pessary removal. A dose of eCG (300 IU; Novormon®, Syntex) was administered at progestagen removal. One shot superovulatory treatment consisted of a single dose of FSH (70 mg NIH-FSH-P1) plus 300 UI of eCG injected at pessary withdrawal. Embryo donors were inseminated by laparoscopy with frozen-thawed semen (100 × 106 spz) 12 h after the onset of estrus. Surgical embryo recovery was done on Day 7 after sponge withdrawal and embryos were graded for quality according to morphology (Grade 1 = excellent or good; Grade 2 = fair; Grade 3 = poor; and Grade 4 = dead or degenerated; IETS 1998). A 2 × 2 factorial ANOVA was used to test the main effects (season and superovulatory treatment) and interactions. There were no significant differences in the proportion of responding ewes (>3 corpora lutea), ovulation rate, and recovered Grades 1 to 2 embryos between the breeding and nonbreeding season (Table 1; P > 0.05). However, number of recovered ova/embryos and ova/embryo recovery rate were higher during the breeding season compared with the nonbreeding season, whereas the percentage of nonfertilized ova was lower in the breeding season than in the nonbreeding season (P < 0.05). Analysis of data comparing superovulatory treatments showed that the proportion of responding ewes, ovulation rate, recovered embryos, and recovered Grades 1 to 2 embryos were lower for the one shot treatment than for the classic treatment (P < 0.05). Embryo recovery rate and nonfertilization rate did not differ between treatments (P > 0.05). It was concluded that there was an increase in the number of total recovered ova/embryos during the breeding season compared with the nonbreeding season, although the number of recovered good-quality embryos was not affected. The use of multiple FSH injections produced a higher number of total recovered and viable embryos in Merino sheep than the one shot superovulatory treatment. Table 1.Embryo yields in ewes submitted to superovulation

Effects of cloprostenol and flurogestone acetate sponge on embryo yields and quality in indigenous ewes in Bangladesh

2014 ◽  
Vol 54 (10) ◽  
pp. 1605 ◽  
Author(s):  
B. F. Zohara ◽  
Azizunnesa ◽  
M. F. Islam ◽  
M. G. S. Alam ◽  
F. Y. Bari
Keyword(s):  
Recovery Rate ◽  
Group Iv ◽  
Corpora Lutea ◽  
Group Iii ◽  
Embryo Recovery ◽  
Fertilisation Rate ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Mean Time

The effects of two doses of cloprostenol and two doses of flurogestone acetate sponge on the onset of oestrus, and embryo recovery and quality were evaluated. Thirty-two indigenous ewes (Wera breed) were allocated into four groups of eight. All ewes were synchronised with 100 µg (Group I) or 175 µg (Group II) cloprostenol injection, 9 days apart, or insertion of intravaginal sponges containing 30 mg (Group III) or 45 mg (Group IV) flurogestone acetate (FGA) for 12 days. The ewes were superovulated with 600 IU PMSG intramuscularly 10 days after the second cloprostenol injection or immediately after sponge removal on Day 12. After the detection of oestrus, the ewes were mated naturally at 6 and 12 h, and some ewes were inseminated laparoscopically. Embryos were recovered surgically 5 or 6 days after service. All ewes exhibited oestrus. The onset of oestrus occurred significantly (P < 0.05) earlier in FGA-treated (50.0 ± 1.5 and 48.0 ± 0.00 h) than in cloprostenol-treated groups. There were no significant differences (P > 0.05) in the mean time of onset of oestrus (50.0 ± 1.5 and 48.0 ± 0.00 h) between the two doses of cloprostenol. The mean number of corpora lutea (8.1 ± 1.26) and embryos recovered (6.1 ± 1.00) was significantly (P < 0.05) higher in ewes treated with 45 mg FGA than in ewes treated with cloprostenol. Embryo recovery rate was significantly (P < 0.05) higher in ewes treated with 45 mg FGA (75.4%) than in other groups (41.7% and 51.6% in 100 μg and 175 μg cloprostenol- and 52.7% in 30 mg FGA-treated groups, respectively). Fertilisation rate was 93.3% in ewes given 100 µg cloprostenol, whereas other groups showed 100% fertilisation rate. The highest percentage (100%) of Grade 1 embryos was in FGA groups. In conclusion, despite FGA protocol presenting superior results, cloprostenol protocol was equally efficient in synchronising oestrus. The embryo recovery rate was better after 45 mg FGA than 30 mg FGA or after either dose of cloprostenol.

REPRODUCTIVE PERFORMANCE IN EWES ON A LOW-SELENIUM DIET

1975 ◽  
Vol 55 (4) ◽  
pp. 513-517 ◽  
Author(s):  
D. MITCHELL ◽  
M. HIDIROGLOU ◽  
K. J. JENKINS
Keyword(s):  
Adverse Effect ◽  
Sex Ratio ◽  
Reproductive Performance ◽  
Embryonic Mortality ◽  
Group Differences ◽  
Corpora Lutea ◽  
Conception Rates ◽  
Group 2 ◽  
Low Selenium ◽  
Group 1

Sixty-four Selenium (Se)-deficient ewes (group 1) and 65 Se-adequate ewes (group 2) were exposed to fertile, harnessed rams for a 42-day period (1 October–12 November 1973). Between 26 October and 7 December, approximately half of the animals in each group were slaughtered 25–30 days after their last marked mating to determine numbers of embryos and corpora lutea (CL). The percentages of ewes pregnant and CL not represented by normal embryos were 80.6 and 12.8 in group 1 and 78.8 and 34.1 in group 2 and the average number of CL/ewe was 1.57 and 1.63, respectively. The remaining ewes were kept until anticipated time of lambing. The percentage of ewes which lambed and the average number of lambs/ewe were 67.9 and 1.47 (group 1) and 71.9 and 1.44 (group 2). No group differences in sex ratio of lambs was observed. Composite conception rates for the slaughtered and lambed ewes were 76.2% for the Se-deficient and 76.6% for the Se-adequate animals. It was concluded that under the conditions of this experiment Se depletion had no adverse effect on ewe conception rates, embryonic mortality or numbers of lambs born.

411 EFFICIENCY OF DAY SEVEN COLLECTION OF BOVINE EMBRYOS AFTER SUPEROVULATION BY FLUSHING THE OVIDUCTS AND THE UTERINE HORNS

2010 ◽  
Vol 22 (1) ◽  
pp. 363
Author(s):  
V. Havlicek ◽  
A. Kuzmany ◽  
J. F. Beckers ◽  
B. Remy ◽  
G. Brem ◽  
...  
Keyword(s):  
Body Weight ◽  
The Netherlands ◽  
Epidural Anesthesia ◽  
Recovery Rate ◽  
Developmental Rate ◽  
Corpora Lutea ◽  
First Flush ◽  
Bovine Embryos ◽  
Embryo Recovery ◽  
Left And Right

Embryo collection on Day 7 following superovulation is characterized by wide variation in the number of ova/embryos recovered. The differences between the numbers of corpora lutea and recovered ova/embryos provide evidence for incomplete recovery. The aim of this study was to examine the effect of flushing only the uterine horns v. endoscopical flushing of both the oviducts and the uterine horns on embryo recovery rates. A total of 12 superovulated Simmental heifers, aged 18 to 24 months were presynchronized by i.m. administration of PGF2 (500 μg of cloprostenol; Estrumate®, Essex Tierarznei, Munich, Germany) twice within 11 days. Two days after each of the PGF2 treatments, the animals received 0.02 μg of GnRH (Receptal®, Intervet, Boxmeer, the Netherlands). Between Days 9 and 11 of the estrous cycle, the animals got the first of 8 consecutive, twice-daily FSH injections in decreasing doses (in total 300-400 mg of FSH equivalent according to body weight; Stimufol®, ULg FMV PhR, Tilman, Belgium). Two PGF2 treatments were administered 60 and 72 h after the initial FSH treatment. Finally, 48 h after the first PGF2 application, ovulation was induced by injecting 0.02 μg of GnRH simultaneously with the AI. The AI was performed by using 1 straw of semen from a Simmental bull of proven fertility. Seven days after estrus, the heifers were restrained and an epidural anesthesia was performed. The uterine horns of the heifers were first flushed nonsurgically (300 mL of PBS + 1% FCS). Additionally, the oviducts were flushed by a transvaginal endoscopic technique (50 mL of PBS+1% FCS) followed by repeated flushing (250 mL of PBS + 1% FCS) of the uterine horns (combined flushing). In total, 12 animals delivered 132 ova/embryos (mean = 11 ± 6.5; recovery rate = 83%), with 83 developed to blastocysts/morulae (mean = 6.9 ± 6.4). The first flush of the uterine horns gave 3.8 ova/embryos on average with 2.8 being blastocysts/morulae (developmental rate = 75.6%). The following combined flush gave 7.3 ova/embryos with 4.1 developed to blastocysts/morulae (developmental rate = 56.3%). No differences were found between flushing the left and right sides. In conclusion, the present study shows that the additional step involving flushing of oviducts plus uterine horns of superovulated heifers provided recovery of a higher number of ova/embryos. It is assumed that these extra numbers might be caused by the flushing of (1) the uterine horns repeatedly, (2) the region of the utero-tubal junctions, and (3) ova/embryos from the oviducts, probably as a result of incomplete migration.

148 Superovulation and embryo production response in oestrus-synchronized Arunachali yak

2021 ◽  
Vol 33 (2) ◽  
pp. 182
Author(s):  
M. Hussain ◽  
K. Ahmed ◽  
P. Chakravarty ◽  
V. Paul ◽  
B. C. Deka ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Corpora Lutea ◽  
Treatment Variable ◽  
Nutritional Security ◽  
Treatment Groups ◽  
Embryo Recovery ◽  
Reproductive Techniques ◽  
Treatment Responses ◽  
Production Response ◽  
Different Doses

The Arunachali breed of yak is reared by the Monpa pastoral community of Arunachal Pradesh in India for their livelihood and nutritional security on highland pastures. In addition to fulfilling the basic needs of highlanders, this animal is associated with poor production potential linked to its inherent reproductive problems, including silent oestrus, seasonality, and inbreeding, making yak rearing a less-profitable venture. In the recent past, efforts have been made to improve reproductive efficiency of Arunachali yak through assisted reproductive techniques. However, a few regimens for superovulation have been tried to date with limited success in terms of superovulatory response and embryo recovery. Therefore, the present study was undertaken to evaluate the efficacy of two different doses and regimes of superovulation: Stimufol (400 and 200µg per animal) and Folligon (1500 and 1000IU per animal) in Arunachali yak. Twenty-four yaks were allotted in 4 groups (of 6 animals each) and subjected to respective superovulation treatment. Thereafter, the treatment response was assessed in terms of expression of oestrus, interval between treatment and oestrus onset, duration of oestrous, number of corpora lutea and embryos recovered, and analysed using two-way statistical analysis between treatment groups. The results (Table 1) showed a significant (P&lt;0.01) variation in onset of oestrus after the treatment, whereas other responses did not differ among the groups. Further, the recovered embryos were categorized as excellent, good, fair, and poor according to cell size, shape, texture, and number of vesicles present in the blastomeres; 58.33, 20.83, 8.33, and 12.5% embryos in these categories were recovered, respectively. The overall embryo recovery rate was 30.77%, ranging from 10.53 to 52.38% in different treatment groups. The numbers of embryos recorded were less than the numbers of CL present, showing the possibility of non-availability of all ovulated oocytes for fertilization in the oviduct due to untrapping by the fimbriae. The effectiveness of treatments depended upon the precision of oestrus detection and the time of ovulation. In our study, the variation in onset of oestrus might be associated with poor expression of behavioural oestrus in the treated animals. Based on the present findings, we conclude that all the treatments are effective in superovulation and embryo recovery. However, the use of Folligon at 1500IU seemed to be comparatively more effective in Arunachali yak. Table 1. Mean (n=6) average of treatment responses in yak following different superovulatory treatment Variable Treatment Stimufol Folligon 400 µg/animal 200 µg/animal 1500 IU/animal 1000 IU/animal Oestrus response (%) 100 100 100 100 Interval between treatment and oestrus onset (h) 17.67±0.56a 20.83±0.79b 21.17±0.79bc 23.67±1.43c Duration of oestrous (h) 30.33±1.09 28.50±0.99 31.50±1.50 28.83±1.25 Corpora lutea (n) 2.83±1.22 3.50±1.18 3.50±0.67 3.17±0.87 Embryo recovered (n) 0.83±0.83 1.00±0.81 1.83±0.98 0.33±0.21 Recovery rate (%) 29.41 28.57 52.38 10.53 a–cMeans within a row with different superscripts differ P&lt;0.05.

scholarly journals A new technique for nonsurgical embryo recovery in superovulated ewes treated with estradiol and oxytocin

2011 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
O. I. Azawi
Keyword(s):  
Ovarian Response ◽  
Practical Method ◽  
Laparoscopic Technique ◽  
Corpora Lutea ◽  
Cervical Canal ◽  
Transfer Programs ◽  
Embryo Recovery ◽  
Significant Difference ◽  
Group 2 ◽  
A New Technique

The development of an efficient and practical method for transcervical collection of embryos in Awassi ewes to the improvement of embryo recovery was investigated. Twenty-four non-pregnant Awassi ewes of 3-4 years of age were randomly allocated into two groups (n = 12). Each ewe was treated with a progesterone impregnated intra-vaginal sponge for 12 days. The following superovulation treatment was used: ewes received 1200 IU of eCG once as an intramuscular injection 48 h prior to sponge withdrawal. Ovarian response was assessed by determining the number of corpora lutea by laparoscopy at day 6 after mating. Ewes of group 1 (n = 12) their embryos were recovered using the semi-laparoscopic technique. Ewes of group 2 (n = 12) their embryos were recovered using the transcervical technique. All ewes of this group on the evening of day 5 post inseminations, received an i.m. injection of estradiol 17β 2 mg, 12h later, i.v. injection of 10 IU of oxytocin. A bovine teat dilator introduced into the cervical canal and gradually dilated in order to dilate and open the cervical canal with a help of screw provided with the instrument. Results of the present study showed that there was no significant difference in the number of recovered embryos in superovulated ewes when animals treated with estradiol and oxytocin with transcervical technique and semi-laparoscopic technique. It was possible to pass the catheter through the cervix with the aid of bovine teat dilator enhanced by estradiol and oxytocin treatment. In conclusion, the technique for transcervical collection of the embryo from ewes, as described in the present study, may give new encouragement to embryo transfer programs in this species.

Successive in vivo embryo production in Santa Inês sheep

2020 ◽  
Vol 60 (4) ◽  
pp. 497 ◽  
Author(s):  
Pedro Henrique Nicolau Pinto ◽  
Mario Felipe Alvarez Balaro ◽  
Helena Fabiana Reis de Almeida Saraiva ◽  
Viviane Lopes Brair ◽  
Vivian Angélico Pereira Alfradique ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Doppler Ultrasonography ◽  
Corpora Lutea ◽  
Colour Doppler ◽  
Embryo Production ◽  
Previous Response ◽  
Embryo Recovery ◽  
Colour Doppler Ultrasonography ◽  
Santa Inês

Context In vivo embryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, and thus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock. Aims This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterion to select ewes for in vivo embryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL) can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can be used to calculate the recovery rate. Methods Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH and natural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography. Key results The number of CL significantly decreased (P &lt; 0.05) after SOV1 (7.5 ± 4.8) to 3.0 ± 5.0 at SOV 2 and 2.2 ± 3.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r = 0.86, r2 = 0.74; P &lt; 0.0001) and viable embryos (r = 0.79, r2 = 0.63; P &lt; 00001). However, no correlations were observed between SOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response (≤6, 7–10, &gt;10 CL) or between the methods used to quantify CL. Conclusions Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. The intensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Doppler ultrasonography can be used to calculate the recovery rate. Implications Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate is homogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess the recovery rate, improving animal welfare in MOET programs.

Sign in / Sign up

Export Citation Format

Share Document