scholarly journals LMP-1 IMMUNOHISTOCHEMICAL DETECTION OF EPSTEIN - BARR VIRUS IN LYMPHOMA AMONG SUDANESE PATIENTS

2015 ◽  
Vol 3 (9) ◽  
pp. 63-69
Author(s):  
Ibrahim BakhitYousif ◽  
Yousif Mohammed Yousif ◽  
SideegAbd Elaziz Mohammed
Keyword(s):  
Epstein Barr Virus ◽  
Sufficient Evidence ◽  
Control Group ◽  
Barr Virus ◽  
Formalin Fixed Paraffin ◽  
Significant Difference ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Lymphoma Study Group ◽  
And Control

Several studies have suggested a probable etiologic association between Epstein-Barr virus (EBV) and lymphoma; multiple EBV proteins can be expressed in infected lymphocytes, among which Latent membrane protein-1(LMP-1) is thought to be most important for malignant transformation, the aim of this study was to investigate the association of LMP-1 EBV in this tumors. This study was carried at the Radio Isotope Center Khartoum (RICK)- Khartoum state –Sudan from January 2015 to July 2015, it was retrospective and prospective,  the study include  ninety (90) Formalin –fixed paraffin embedded biopsies from which  seventy (70) of malignant lymphoma (study group)  and twenty (20) lymph node hyperplasia  (control group ). EBV was detected in (32%) (23/70), and (10%) (2/20) respectively, the (P.value =0.04), this result showed a significant difference between case and control groups, the, there is no statistically significant difference between the two lymphoma subtypes Hodgkin's and Non-Hodgkin`s the   (P.value= 0.333). There is sufficient evidence for the carcinogenicity of EBV in the causation of lymphoma in Sudan. This requires further evaluation to find out whether this positivity is due to co-infection or has a role in pathogenesis.

scholarly journals Macular Amyloidosis and Epstein-Barr Virus

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Yalda Nahidi ◽  
Naser Tayyebi Meibodi ◽  
Zahra Meshkat ◽  
Narges Nazeri
Keyword(s):  
Epstein Barr Virus ◽  
Globin Gene ◽  
Control Group ◽  
Barr Virus ◽  
Significant Difference ◽  
Macular Amyloidosis ◽  
Ebv Dna ◽  
Epstein Barr ◽  
And Control ◽  
Skin Samples

Background. Amyloidosis is extracellular precipitation of eosinophilic hyaline material of self-origin with special staining features and fibrillar ultrastructure. Macular amyloidosis is limited to the skin, and several factors have been proposed for its pathogenesis. Detection of Epstein-Barr virus (EBV) DNA in this lesion suggests that this virus can play a role in pathogenesis of this disease.Objective. EBV DNA detection was done on 30 skin samples with a diagnosis of macular amyloidosis and 31 healthy skin samples in the margin of removed melanocytic nevi by using PCR.Results. In patients positive for beta-globin gene in PCR, BLLF1 gene of EBV virus was positive in 23 patients (8 patients in case and 15 patients in the control group). There was no significant difference in presence of EBV DNA between macular amyloidosis (3.8%) and control (23.8%) groups (P=0.08).Conclusion. The findings of this study showed that EBV is not involved in pathogenesis of macular amyloidosis.

scholarly journals Frequency of Epstein–Barr Virus DNA in Formalin-fixed Paraffin-embedded Tissue of Patients with Ductal Breast Carcinoma

2019 ◽  
Vol 20 (3) ◽  
pp. 687-692 ◽  
Author(s):  
Chia Sharifpour ◽  
Manoochehr Makvandi ◽  
Alireza Samabafzadeh ◽  
Abdolhassan Talaei-Zadeh ◽  
Nastaran Ranjbari ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Ductal Breast Carcinoma ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

scholarly journals High prevalence of Epstein-Barr virus in the Reed-Sternberg cells of Hodgkin's disease occurring in Peru

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 496-501 ◽  
Author(s):  
KL Chang ◽  
PF Albujar ◽  
YY Chen ◽  
RM Johnson ◽  
LM Weiss
Keyword(s):  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Lower Percentage ◽  
Double Labeling ◽  
Barr Virus ◽  
Formalin Fixed Paraffin ◽  
High Prevalence ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr

Abstract The Epstein-Barr virus (EBV) has been implicated in the pathogenesis of Hodgkin‧s disease (HD). This study was undertaken to determine whether the association of EBV with HD showed geographical variation, as in Burkitt‧s lymphoma. We studied 32 formalin-fixed, paraffin-embedded cases of HD occurring in Peru. EBV DNA-RNA in situ hybridization was performed using a 30-base biotinylated antisense oligonucleotide complementary to the EBER1 gene of EBV. EBV immunohistochemistry was also performed, using a monoclonal antibody (MoAb) to the latent membrane protein (LMP1) of EBV. Identification of the precise cellular subset staining with EBV was accomplished via double-labeling with MoAbs directed against Reed-Sternberg cells (LeuM1/CD15) and B cells (L26/CD20). EBV RNA was identified in all or virtually all of the Reed- Sternberg cells and variants in 30 of the 32 (94%) cases of HD by in situ hybridization. LMP1 expression was identified in 83% of the EBER1- positive cases. Double-labeling studies confirmed the localization of EBV RNA to CD15-expressing Hodgkin‧s cells. This study found an extremely high prevalence of EBV in Peruvian HD, in contrast to the much lower percentage of EBV-associated cases of HD occurring in “Western” patients.

scholarly journals Association of Epstein–Barr Virus and Cytomegalovirus Infections with Esophageal Carcinoma

2021 ◽  
Vol 14 (10) ◽  
Author(s):  
Mahin Ahangar Oskouee ◽  
Jamal Sarvari ◽  
Afagh Moattari ◽  
Ahmad Habibi ◽  
Amir Taher Eftehkar Sadat
Keyword(s):  
Esophageal Carcinoma ◽  
Epstein Barr Virus ◽  
Viral Infections ◽  
Control Group ◽  
Control Groups ◽  
Barr Virus ◽  
Potential Association ◽  
Epstein Barr ◽  
And Control ◽  
Cytomegalovirus Infections

Background: Given the fact that viral infections play an important role, either directly or indirectly, in around 20 percent of human cancers, this study aimed at investigating the potential association of Epstein–Barr virus (EBV) and cytomegalovirus (CMV) infections in esophageal cancer that is the sixth most common cause of cancer-related deaths. Methods: In this case-control study, a total of 200 paraffin-embedded biopsies of cancerous and benign esophageal tissues were gathered from the biopsy bank of Imam Reza Hospital, Tabriz, Iran in 2017. All samples were first deparaffinized, and then subjected to commercial DNA extraction. The quality of extracted DNA was evaluated by amplification of the beta globulin gene. Identification of EBV and CMV DNA was performed using primers designed for the EBER region of EBV and the immediate early (IE) region of the CMV genome, respectively. Results: The mean age of the subjects in the test and control groups was 52.2 (17.1) and 59.9 (18.9), respectively. The distribution of gender (male/female) in patient and control groups was 54/46 and 53/47, respectively. Our results showed that the frequency of EBV (P < 0.001) and CMV (P < 0.001) in cancerous samples was statistically higher than control group. Moreover, in the cancerous group the rate of EBV was significantly higher in the esophageal adenocarcinomas (EAC) sample (12 out of 70) than esophageal squamous cell carcinomas (ESCC) (0 out of 30) (P = 0.016) but, in the ESCC group, 17 out of 30 subjects were positive for CMV which was significantly higher in comparison with EAC patients (1 out of 70) (P < 0.001). Conclusions: Findings indicated that EBV and CMV might be contributed to the pathogenesis of EAC and ESCC types of esophageal carcinoma, respectively, although further studies are warranted.

scholarly journals Molecular Detection of Epstein–Barr Virus, Human Herpes Virus 6, Cytomegalovirus, and Hepatitis B Virus in Patients with Multiple Sclerosis

2020 ◽  
Vol 12 (3) ◽  
pp. 171-177
Author(s):  
Mohsen Asouri ◽  
Mohammad Ali Sahraian ◽  
Morteza Karimpoor ◽  
Sadegh Fattahi ◽  
Nima Motamed ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Control Group ◽  
Case Group ◽  
Control Groups ◽  
Barr Virus ◽  
Human Herpes Virus ◽  
B Virus ◽  
Human Herpes Virus 6 ◽  
Epstein Barr ◽  
And Control

BACKGROUND Multiple sclerosis (MS) is a chronic disease with significant morbidity. A wide spectrum of risk factors has been suggested that triggers the development of MS. Among them, several viral infections have been implicated to play a role in MS pathogenesis. We aimed to evaluate the relationship between viral diseases, including Epstein–Barr virus (EBV), human herpes virus 6 (HHV-6), cytomegalovirus (CMV), and hepatitis B virus (HBV) and MS in the present case-control study. METHODS About 100 patients with confirmed MS and age- and sex-matched individuals were selected as case and control groups, respectively. The patients were randomly selected from individuals diagnosed by neurologists based on the clinical signs and symptoms and imaging procedures. RESULTS More than 100 patients with MS and patients who were referred for other causes were analyzed for the presence of DNA of EBV, HHV6, CMV, and HBV separately. 9.37% of the control group had a positive test for the DNA of EBV in a real-time polymerase chain reaction (PCR), while the frequency of positive test result was zero in the case group (p = 0.0012). HBV DNA was not detected in both the case and control groups. The prevalence of CMV was 0.88 and zero in the control and case groups, respectively (p = 0.3410). For HHV6, 9.73 % of the control group had a positive result, while this test was positive in 5.88% of the patients with MS (p = 0.2959). CONCLUSION We detected a significantly higher number of individuals with DNA of EBV in their blood among the control group compared with the case group. In conclusion, the results suggest a surprisingly adverse association between MS and EBV, and no association was found between the presence of DNA of HBV, CMV, and HHV6 and MS.

scholarly journals Determination of the Expression of Latent Epstein Barr Virus in Omani Nasopharyngeal Carcinoma Patients

2021 ◽  
Vol 14 (1) ◽  
pp. 257-265
Author(s):  
Sheikha Nasser said Al-Shidhani ◽  
Shadia Al-Sinawi ◽  
Maiya Al-Bahri ◽  
Masoud Al-Kindi ◽  
Mohamed Mabruk
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Tissue Samples ◽  
Barr Virus ◽  
Carcinoma Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

Background: Nasopharyngeal carcinoma(NPC) is a rare malignant carcinoma that develops in the epithelial lining of the nasopharyngeal mucosa It is the most common neoplasm of the Nasopharynxand it is associated with many risk factors; one of them is Epstein-Barr virus infection. An Epstein-Barr virus is a tumorigenic herpes virus that infects and persists in B-lymphocytes without causing disease. This virus is associated with significant pathological conditions, such as benign and malignant lymphoproliferation. Objectives: To determine Epstein -Barr encoded RNA 1&2 (EBER1,2) and latent membrane protein (LMP) expressionin formalin-fixed paraffin-embedded tissue samples obtained from Omani patients diagnosed with nasopharyngeal carcinoma.Also, to identify the pattern and the type(s) of cells infected with EBV in nasopharyngeal carcinoma tissue samples obtained from Omani patients. Moreover, to compare the sensitivity of Immuno histochemistry and in-situ Hybridization for the detection of EBV in the nasopharyngeal carcinoma tissue sample. Materials and Method:Thirteen formalin-fixed paraffin-embedded nasopharyngeal carcinoma tissue samples archived from the period 2010 to 2017, were obtained from the Pathology Departments of Sultan Qaboos University Hospital and the Armed Force Hospital. These tissue samples were processed using two different methods Immunohistochemistry (IHC) and In situ hybridization (ISH). Results:Eleven out of thirteen NPC Omani patients were positive for EBV (84.61%) by either LMP-IHC or EBER-ISH. All cells stained positive for EBV in NPC tissue samples was of malignant type rather than normal cell type. EBV is mostly detected in patients in the age group of less than 50 years old. Also out of the 13 NPC patients, seven females (58.34%), and six males (46.15%) were positive for EBV. Conclusion:This study may provide evidence indicating an association between EBV and nasopharyngeal carcinoma. In addition, the detection of EBV in NPC obtained from Omani patients may encourage the physician to consider using anti-herpes virus drugs in the treatment of EBV positive NPC patients as an additional tool for the treatment of this kind of malignancy.

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