Investigation of The Antiproliferative Effect of Camelia Sinensis on Liver Cancer (Hepg2) Cell Line

Objective: To explore the anticancer activity of the flowers of Argemone mexicana L. against the human hepatoma cell line (HepG2).Methods: In vitro anticancer activity was carried out to screen cytotoxicity effectiveness of the solid obtained from ethyl acetate fraction of A. mexicana L. flower extract at different concentrations against the HepG2 cell line. The MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay for cell viability and markers is expected to confirm the cytotoxicity.Result: Ethyl acetate fraction from the flower extract of A. mexicana L. was tested for its anticancer activity against HepG2 cell lines (liver cancer) at various concentrations by MTT assay. It was confirmed that the IC50 value of this sample was 72±1.7 μg/ml against liver cancer HepG2 cell line.Conclusions: A. mexicana L. is a potential plant with anticancer activity. The isolation of the pure compounds and determination of the structure of individual compounds will be further performed.

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The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

International Journal of Molecular Sciences ◽

10.3390/ijms222313135 ◽

2021 ◽

Vol 22 (23) ◽

pp. 13135

Author(s):

Viktoriia A. Arzumanian ◽

Olga I. Kiseleva ◽

Ekaterina V. Poverennaya

Keyword(s):

Liver Cancer ◽

Cell Line ◽

Hepg2 Cell ◽

Cell Lines ◽

Molecular Mechanisms ◽

Basic Research ◽

Health Concern ◽

Hepg2 Cell Line ◽

Wide Range

Liver cancer is the third leading cause of cancer death worldwide. Representing such a dramatic impact on our lives, liver cancer is a significant public health concern. Sustainable and reliable methods for preventing and treating liver cancer require fundamental research on its molecular mechanisms. Cell lines are treated as in vitro equivalents of tumor tissues, making them a must-have for basic research on the nature of cancer. According to recent discoveries, certified cell lines retain most genetic properties of the original tumor and mimic its microenvironment. On the other hand, modern technologies allowing the deepest level of detail in omics landscapes have shown significant differences even between samples of the same cell line due to cross- and mycoplasma infection. This and other observations suggest that, in some cases, cell cultures are not suitable as cancer models, with limited predictive value for the effectiveness of new treatments. HepG2 is a popular hepatic cell line. It is used in a wide range of studies, from the oncogenesis to the cytotoxicity of substances on the liver. In this regard, we set out to collect up-to-date information on the HepG2 cell line to assess whether the level of heterogeneity of the cell line allows in vitro biomedical studies as a model with guaranteed production and quality.

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IN VITRO EVALUATION OF ANTIDIABETIC AND CYTOTOXICITY POTENTIALS OF THE RHIZOME EXTRACT OF DRYNARIA QUERCIFOLIA (L.) J. SMITH

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i11.35078 ◽

2019 ◽

pp. 72-76

Author(s):

PRASANNA G ◽

DEVI R ◽

ISHWARYA G

Keyword(s):

Liver Cancer ◽

Glucose Uptake ◽

Cell Line ◽

Hepg2 Cell ◽

Plant Extract ◽

Mtt Assay ◽

Hepg2 Cell Line ◽

Uptake Assay ◽

Glucose Uptake Assay

Objective: In the present study, an attempt has been made to evaluate the in vitro antidiabetic and cytotoxic potentials of the rhizome extract of Drynaria quercifolia (L.) J. Smith. Methods: In vitro antidiabetic activity was determined by two different assays such as alpha-amylase inhibition assay and glucose uptake assay. The plant extract with three different concentrations was used for this assay. L6 rat myogenic cells were selected and subjected to glucose uptake assay. The cytotoxic activity of the different concentrations of the plant extract on HepG2 cell line was also investigated in vitro through 3-(4,5, dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: The findings of the study provide evidence that the rhizome extract of D. quercifolia possesses significant anti-diabetic activity. In MTT assay, the significant cytotoxic effect of plant extract was observed by measuring the percentage of cell viability on the HepG2 cell line. Conclusion: The findings indicated that rhizome extracts of D. quercifolia have potential as a medicinal drug against diabetes mellitus (DM) and liver cancer. Further, studies with in vivo and clinical trials need to be conducted to establish rhizome extract as a safe agent for DM and liver cancer therapy.

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ANTIPROLIFERATIVE ACTIVITY OF QUERCETIN ON HepG2 CELL LINE INDUCED LIVER CANCER IN RATS

World Journal of Pharmacy and Pharmaceutical Sciences ◽

10.20959/wjpps20174-8835 ◽

2017 ◽

pp. 1138-1155

Author(s):

V. Uma Rani

Keyword(s):

Liver Cancer ◽

Cell Line ◽

Hepg2 Cell ◽

Antiproliferative Activity ◽

Hepg2 Cell Line

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Flavonoids from Enicostema littorale blume enhances glucose uptake of cells in insulin resistant human liver cancer (HepG2) cell line via IRS-1/PI3K/Akt pathway

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.03.047 ◽

2017 ◽

Vol 90 ◽

pp. 268-277 ◽

Cited By ~ 22

Author(s):

Priyanka Mokashi ◽

Aparna Khanna ◽

Nancy Pandita

Keyword(s):

Liver Cancer ◽

Glucose Uptake ◽

Cell Line ◽

Hepg2 Cell ◽

Human Liver ◽

Akt Pathway ◽

Hepg2 Cell Line ◽

Insulin Resistant ◽

Human Liver Cancer

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Garcinia dulcisFruit Extract Induced Cytotoxicity and Apoptosis in HepG2 Liver Cancer Cell Line

BioMed Research International ◽

10.1155/2015/916902 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Mohd Fadzelly Abu Bakar ◽

Nor Ezani Ahmad ◽

Monica Suleiman ◽

Asmah Rahmat ◽

Azizul Isha

Keyword(s):

Liver Cancer ◽

Cell Line ◽

Hepg2 Cell ◽

Cancer Cell ◽

Cancer Cell Line ◽

Annexin V ◽

Dependent Manner ◽

Liver Cancer Cell ◽

Hepg2 Cell Line ◽

Liver Cancer Cell Line

Garcinia dulcisor locally known in Malaysia as “mundu” belongs to the family of Clusiaceae. The study was conducted to investigate the anticancer potential of different parts ofG. dulcisfruit extracts and their possible mechanism of action in HepG2 liver cancer cell line. MTT assay showed that the peel, flesh, and seed extracts ofG. dulcisinduced cytotoxicity in HepG2 cell line with IC50values of 46.33 ± 4.51, 38.33 ± 3.51, and 7.5 ± 2.52 µg/mL, respectively. The flesh extract ofG. dulcisinduced cell cycle arrest at sub-G1(apoptosis) phase in a time-dependent manner. Staining with Annexin V-FITC and propidium iodide showed that 41.2% of the cell population underwent apoptosis after 72 hours of exposure of the HepG2 cell line toG. dulcisflesh extract. Caspase-3 has been shown to be activated which finally leads to the death of HepG2 cell (apoptosis). GC-MS analysis showed that the highest percentage of compound identified in the extract ofG. dulcisflesh was hydroxymethylfurfural and 3-methyl-2,5-furandione, together with xanthones and flavonoids (based on literature), could synergistically contribute to the observed effects. This finding suggested that the flesh extract ofG. dulcishas its own potential as cancer chemotherapeutic agent against liver cancer cell.

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