scholarly journals ANTICANCER ACTIVITY OF ARGEMONE MEXICANA L (FLOWERS) AGAINST HUMAN LIVER CANCER (HEPG2) CELL LINE

2017 ◽  
Vol 10 (10) ◽  
pp. 351
Author(s):  
Prabhakaran D ◽  
Senthamilselvi Mm ◽  
Rajeshkanna A
Keyword(s):  
Liver Cancer ◽  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Ethyl Acetate Fraction ◽  
Hepatoma Cell Line ◽  
Hepg2 Cell Line ◽  
Argemone Mexicana ◽  
Flower Extract

  Objective: To explore the anticancer activity of the flowers of Argemone mexicana L. against the human hepatoma cell line (HepG2).Methods: In vitro anticancer activity was carried out to screen cytotoxicity effectiveness of the solid obtained from ethyl acetate fraction of A. mexicana L. flower extract at different concentrations against the HepG2 cell line. The MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay for cell viability and markers is expected to confirm the cytotoxicity.Result: Ethyl acetate fraction from the flower extract of A. mexicana L. was tested for its anticancer activity against HepG2 cell lines (liver cancer) at various concentrations by MTT assay. It was confirmed that the IC50 value of this sample was 72±1.7 μg/ml against liver cancer HepG2 cell line.Conclusions: A. mexicana L. is a potential plant with anticancer activity. The isolation of the pure compounds and determination of the structure of individual compounds will be further performed.

Activation of apoptosis by ethyl acetate fraction of ethanol extract of Dianthus superbus in HepG2 cell line

2012 ◽  
Vol 36 (1) ◽  
pp. e40-e45 ◽  
Author(s):  
Jian-Qing Yu ◽  
Yan Yin ◽  
Jia-Chuan Lei ◽  
Xiu-Qiao Zhang ◽  
Wei Chen ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hepg2 Cell Line

scholarly journals The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

2021 ◽  
Vol 22 (23) ◽  
pp. 13135
Author(s):  
Viktoriia A. Arzumanian ◽  
Olga I. Kiseleva ◽  
Ekaterina V. Poverennaya
Keyword(s):  
Liver Cancer ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Cell Lines ◽  
Molecular Mechanisms ◽  
Basic Research ◽  
Health Concern ◽  
Hepg2 Cell Line ◽  
Wide Range

Liver cancer is the third leading cause of cancer death worldwide. Representing such a dramatic impact on our lives, liver cancer is a significant public health concern. Sustainable and reliable methods for preventing and treating liver cancer require fundamental research on its molecular mechanisms. Cell lines are treated as in vitro equivalents of tumor tissues, making them a must-have for basic research on the nature of cancer. According to recent discoveries, certified cell lines retain most genetic properties of the original tumor and mimic its microenvironment. On the other hand, modern technologies allowing the deepest level of detail in omics landscapes have shown significant differences even between samples of the same cell line due to cross- and mycoplasma infection. This and other observations suggest that, in some cases, cell cultures are not suitable as cancer models, with limited predictive value for the effectiveness of new treatments. HepG2 is a popular hepatic cell line. It is used in a wide range of studies, from the oncogenesis to the cytotoxicity of substances on the liver. In this regard, we set out to collect up-to-date information on the HepG2 cell line to assess whether the level of heterogeneity of the cell line allows in vitro biomedical studies as a model with guaranteed production and quality.

scholarly journals IN VITRO EVALUATION OF ANTIDIABETIC AND CYTOTOXICITY POTENTIALS OF THE RHIZOME EXTRACT OF DRYNARIA QUERCIFOLIA (L.) J. SMITH

2019 ◽  
pp. 72-76
Author(s):  
PRASANNA G ◽  
DEVI R ◽  
ISHWARYA G
Keyword(s):  
Liver Cancer ◽  
Glucose Uptake ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Plant Extract ◽  
Mtt Assay ◽  
Hepg2 Cell Line ◽  
Uptake Assay ◽  
Glucose Uptake Assay

Objective: In the present study, an attempt has been made to evaluate the in vitro antidiabetic and cytotoxic potentials of the rhizome extract of Drynaria quercifolia (L.) J. Smith. Methods: In vitro antidiabetic activity was determined by two different assays such as alpha-amylase inhibition assay and glucose uptake assay. The plant extract with three different concentrations was used for this assay. L6 rat myogenic cells were selected and subjected to glucose uptake assay. The cytotoxic activity of the different concentrations of the plant extract on HepG2 cell line was also investigated in vitro through 3-(4,5, dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: The findings of the study provide evidence that the rhizome extract of D. quercifolia possesses significant anti-diabetic activity. In MTT assay, the significant cytotoxic effect of plant extract was observed by measuring the percentage of cell viability on the HepG2 cell line. Conclusion: The findings indicated that rhizome extracts of D. quercifolia have potential as a medicinal drug against diabetes mellitus (DM) and liver cancer. Further, studies with in vivo and clinical trials need to be conducted to establish rhizome extract as a safe agent for DM and liver cancer therapy.

GC-analysis, and Antioxidant, Anti-inflammatory, and Anticancer Activities of Some Extracts and Fractions of Linum usitatissimum

2020 ◽  
Vol 16 (9) ◽  
pp. 1306-1318
Author(s):  
Eman A. Morsi ◽  
Hend O. Ahmed ◽  
Heba Abdel-Hady ◽  
Mortada El-Sayed ◽  
Mohamed A. Shemis
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Cell Line ◽  
Linum Usitatissimum ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Anti Inflammatory ◽  
Phenolic And Flavonoid

Context: Linum usitatissimum (Linn); known as Flaxseed, is one of the most important medicinal plants traditionally used for health benefits and also as nutritional purposes. Objective: Estimation of total phenolic and flavonoid contents, and evaluation of the antioxidant, antiinflammatory and anticancer activities have been performed on hexane, ethyl acetate, n-butanol, and methanol extracts and also on fractions of methanol extract (hexane, ethyl acetate, and n-butanol). Materials and Methods: Phenolic and flavonoid contents were detected using spectrophotometric and colorimetric assays. Antioxidant and anti-inflammatory activities were estimated in-vitro. Anticancer activity of extracts was tested on Hepatocellular carcinoma cell line (HepG2) and breast cancer cell line (MCF7). Results: The methanolic extract and its ethyl acetate fraction showed higher contents of total phenols and flavonoids. Also, the methanolic extract showed a higher antioxidant activity. The butanolic and ethyl acetate fractions at concentration 500μg/ml yielded a higher percent of inhibition of protein denaturation; 87.9% and 90%, respectively. The ethyl acetate fraction and the methanolic extract showed an obvious anticancer activity against HepG2 and MCF7 (IC50=60±0.24 and 29.4±0.12μg. ml-1) and (IC50=94.7±0.21 and 227±0.48μg. ml-1), respectively. The GC-MS analysis showed that the methanolic extract had 32 compounds whereas the ethyl acetate and butanol fractions contained 40 and 36 compounds, respectively. Conclusion: Flaxseed contains different biologically active compounds that have been found to possess various activities, which can protect the human body against several diseases.

scholarly journals Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line

2019 ◽  
Vol 10 (3) ◽  
pp. 131
Author(s):  
Roihatul Mutiah ◽  
Trian Sidha Minggarwati ◽  
Risma Aprinda Kristanti ◽  
Erna Susanti
Keyword(s):  
Cervical Cancer ◽  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Ethyl Acetate Fraction ◽  
Cervical Cancer Cell Line ◽  
Compound Identification ◽  
Hela Cervical Cancer Cell

Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical).Keywords: Compound identification, Anticancer activity, Eleutherine palmifolia (L.) Merr., cervical cancer

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