scholarly journals Respon Pertumbuhan Tanaman Iler (Coleus scutellarioides (L.) Benth) pada Kondisi Cekaman Kekeringan terhadap Inokulasi Fungi Mikoriza Arbuskular (FMA)

2021 ◽  
Vol 12 (1) ◽  
pp. 17-22
Author(s):  
Eva Proditus Sianturi ◽  
Budiman Budiman ◽  
Moh. Ega Elman Miska
Keyword(s):  
Drought Stress ◽  
Leaf Area ◽  
Plant Height ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Optimal Level ◽  
Root Infection ◽  
Number Of Leaves ◽  
Amf Inoculation ◽  
Number Of Branches

Iler plant (Coleus artopurporeus L benth) is a plant that was originally cultivated as an ornamental plant and then developed as a medicinal plant because it contains flavonoids and anthocyanins. Drought stress is a major environmental problem that causes various changes in plant morphological, metabolic and physiological functions. Utilization of Arbuscular Mycorrhizal Fungi (AMF) is an effort to support the growth and development of iler plants in drought stress conditions. This study aims to determine the effect of AMF inoculation on the growth of iler plants under drought stress conditions, determine the optimal level of drought stress for ilher plant growth, and determine the best interaction between AMF inoculation and the level of drought stress on the growth of iler plants. The design used in this study was a completely randomized factorial 2-factor design. The first factor is AMF inoculation consisting of 2 levels, namely, without administration of AMF inoculum and AMF inoculum administration. The second factor is drought stress consists of 3 levels namely, 100% field capacity, 90% drought stress, and 45% drought stress. The results showed the effectiveness of the AMF inoculum test was able to increase the growth of iler plants based on parameters plant height, number of leaves, number of branches, leaf area, root length, root volume, root dry weight, number of spores and root infection. The optimal level of drought stress for iler plant growth is 90% drought stress, it is based on the parameters of plant height, number of leaves, number of branches, leaf area, root length, number of spores and root infection. There is the best interaction between AMF inoculation and 90% drought stress level on the growth of iler plants. These interactions can increase plant height, leaf area, number of spores and root infection. Keywords: arbuscular mycorrhizal fungi (FMA), drought stress, iler plants

scholarly journals Penekanan Perkembangan Penyakit Bercak Ungu pada Bawang Merah oleh Cendawan Mikoriza Arbuskula

2017 ◽  
Vol 12 (5) ◽  
pp. 159
Author(s):  
Marlina Puspita Sari ◽  
Bambang Hadisutrisno ◽  
Suryanti Suryanti
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Plant Height ◽  
Mycorrhizal Fungi ◽  
Biocontrol Agent ◽  
Arbuscular Mycorrhizal ◽  
Higher Plant ◽  
Fungicide Treatment ◽  
Alternaria Sp ◽  
Number Of Leaves ◽  
Amf Inoculation

Arbuscular mycorrhizal fungi (AMF) is known to improve the growth of shallot (Allium cepa var. aggregatum) and strengthen the resistance of plants toward disease infection.  This research aimed to find out the roles of AMF in suppressing the development of purple blotch disease caused by  Alternaria sp. on shallot in Caturtunggal, Sleman, Yogyakarta.  Inoculation of AMF either on fertilization of N, P, K or without fertilization treatment resulted on higher plant height and number of leaves compared to those without AMF inoculation. The plant inoculated with AMF had lower purple blotch disease intensity and disease progression than control and fungicide treatment. The result showed that AMF, in addition to act as the bio-fertilizer, is a potential to be a biocontrol agent.

scholarly journals Arbuscular mycorrhizal technology for the growth enhancement of micropropagated Spilanthes acmella Murr

2012 ◽  
Vol 48 (No. 1) ◽  
pp. 31-36 ◽  
Author(s):  
K. Yadav ◽  
N. Singh ◽  
A. Aggarwal
Keyword(s):  
Survival Rate ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Direct Organogenesis ◽  
Growth Enhancement ◽  
Acaulospora Laevis ◽  
Spilanthes Acmella ◽  
Annual Herb ◽  
Number Of Leaves ◽  
Number Of Branches

Spilanthes acmella (Asteraceae) is an endangered ornamental cum medicinal annual herb. This study reports on the effect of two arbuscular mycorrhizal fungi (Glomus mosseae and Acaulospora laevis) applied either alone or in combination on post-transplanting performance of micropropagated S. acmella. Complete plantlets of S. acmella were raised by direct organogenesis from nodal explants on Murashige and Skoog medium supplemented with various cytokinins. S. acmella plantlets responded to all three mycorrhizal treatments in a significantly different way. G. mosseae enhanced the survival rate of S. acmella plantlets to 100%. Plant height, plant spread, number of branches per plant, number of leaves per plant, leaf area, biomass production, and chlorophyll content were significantly higher in AM inoculated plantlets as compared to the uninoculated ones. This clearly demonstrates that AM inoculation improved not only the survival rate of micropropagated plantlets but also their growth and biomass yield.  

scholarly journals PENGARUH PEMBERIAN CENDAWAN MIKORIZA ARBUSCULAR (CMA) DAN DOSIS PUPUK P TERHADAP PERTUMBUHAN DAN PRODUKSI OKRA (Abelmoschus esculentus L)

2021 ◽  
Vol 3 (2) ◽  
pp. 601-612
Author(s):  
Roni Novianto ◽  
◽  
Sri Hartatik ◽  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Plant Height ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Fruit Weight ◽  
Plant Weight ◽  
Randomized Design ◽  
Two Factors ◽  
P Fertilizer ◽  
Number Of Leaves

Okra is a vegetable crop that has a high selling value and the production of okra is more exported than sold domestically. The demand for okra from year to year continues to increase from various countries, especially Japan, which imports the most okra from Indonesia. Fertilization of phosphorus (P) fertilizer to fulfill nutrients and provision of arbuscular mycorrhizal fungi (CMA) for more efficient and maximum absorption by plants. Phosphorus (P) fertilizer can increase the production and quality of okra. The aim of this study was to determine the combination of treatment of arbuscular mycorrhizal fungi and which dosage of P fertilizer was best to increase the yield of okra production. The design used was a completely randomized design (CRD) with factorial treatment consisting of two factors. The first factor is the dose of arbucular mycorrhizal fungi (CMA) consisting of 4 levels, namely (0; 160; 320; 480) and the second factor is the dose of P fertilizer (75; 100; 125; 150). Parameters observed were plant height, number of leaves per plant, volume of roots, number of fruits per plant, weight of fresh fruit per plant and weight of plant dry corpse. Based on the analysis of variance, it showed that the interaction was significantly different on the variables of root volume, plant height, number of fruits and fruit weight. Based on research, the CMA dose of 320 kg / ha and the dose of 125 kg / ha gave the highest production results.

scholarly journals Pengaruh Fungi Mikoriza Arbuskula dan Kompos Limbah Kakao terhadap Kolonisasi Mikoriza, dan Pertumbuhan Bibit Kakao pada Ultisol

2020 ◽  
Vol 4 (4) ◽  
pp. 657-665
Author(s):  
Raina Muzlifa ◽  
Fikrinda Fikrinda ◽  
Yadi Jufri
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Leaf Area ◽  
Plant Height ◽  
Mycorrhizal Fungi ◽  
Block Design ◽  
Arbuscular Mycorrhizal ◽  
Mycorrhizal Colonization ◽  
Stem Diameter ◽  
Randomized Block Design ◽  
Glomus Sp

Abstrak. Ultisol merupakan salah satu tanah marginal yang memerlukan pengelolaan yang tepat untuk meningkatkan kesuburannya. Penelitian ini bertujuan untuk mengetahui pemberian Fungi Mikoriza Arbuskula (FMA) dan kompos limbah kakao terhadap kolonisasi FMA, dan pertumbuhan bibit kakao pada Ultisol. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) pola faktorial dengan dua faktor perlakuan dan tiga ulangan. Faktor pertama adalah jenis FMA yaitu tanpa FMA (F0), Glomus sp (F1), dan Glomus sp. + Gigaspora sp. (F2). Faktor kedua adalah dosis kompos yaitu 0 ton.ha-1 (K0), 20 ton.ha-1 (K1), dan 30 ton.ha-1(K2). Hasil penelitian menunjukkan bahwa FMA berpengaruh nyata terhadap kolonisasi mikoriza, namun tidak berpengaruh nyata terhadap tinggi tanaman  pada 30, 60, 90 HST, diameter batang pada 30, 60, 90 HST, dan luas daun pada 90 HST.  Pemberian kompos limbah kakao berpengaruh nyata terhadap tinggi tanaman pada 90 HST, namun tidak berpengaruh nyata terhadap tinggi tanaman pada 30 dan 60 HST, diameter batang pada 30, 60 90 HST, dan luas daun pada 90 HST.  Kombinasi FMA dan kompos limbah kakao berpengaruh nyata terhadap diameter batang pada 30 HST namun tidak berpengaruh  nyata terhadap tinggi tanaman pada 30, 60, 90 HST, diameter batang pada 60 dan 90 HST, dan luas daun pada 90 HST. Perlakuan FMA jenis Glomus sp. + Gigaspora sp. dan kompos 20 g.pot-1 memberikan pengaruh terbaik terhadap kolonisasi mikoriza dan pertumbuhan tanaman. The effects of arbuscular mycorrhizal fungi and compost of cocoa waste on myccorrhiza colonization, and the cocoa seedling growth on UltisolAbstract. Ultisol is one of marginal soils which requires proper management to increase its fertility. This study aims to determine the administration of Arbuscular Mycorrhizal Fungi (FMA) and cocoa waste compost to FMA colonization, and the growth of cacao seedlings on Ultisols. This research used factorial randomized block design (RBD) with two treatment factors and three replications. The first factor is the type of FMA that is without FMA (F0), Glomus sp (F1), and Glomus sp. + Gigaspora sp. (F2). The second factor is the compost dose which is 0 tons.ha-1 (K0), 20 tons.ha-1 (K1), and 30 tons.ha-1 (K2). The results showed that FMA significantly affected mycorrhizal colonization, but did not significantly affect plant height at 30, 60, 90 HST, stem diameter at 30, 60, 90 HST, and leaf area at 90 HST. Cocoa waste compost has a significant effect on plant height at 90 HST, but no significant effect on plant height at 30 and 60 HST, stem diameter at 30, 60 90 HST, and leaf area at 90 HST. The combination of AMF and compost of cocoa waste significantly affected the stem diameter at 30 HST but did not significantly affect the plant height at 30, 60, 90 HST, stem diameter at 60 and 90 HST, and leaf area at 90 HST. Treatment of FMA type Glomus sp. + Gigaspora sp. and compost 20 g.pot-1 provides the best effect on mycorrhizal colonization and plant growth.   

scholarly journals Physic nut plants present high mycorrhizal dependency under conditions of low phosphate availability

2011 ◽  
Vol 23 (1) ◽  
pp. 33-44 ◽  
Author(s):  
Elcio Liborio Balota ◽  
Oswaldo Machineski ◽  
Priscila Viviane Truber ◽  
Alexandra Scherer ◽  
Fabio Suano de Souza
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Leaf Area ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Conditions ◽  
Dry Matter Content ◽  
Physic Nut ◽  
Mycorrhizal Dependency ◽  
Soil P ◽  
Amf Inoculation

The physic nut (Jatropha curcas L.) is a perennial tree that occurs naturally in the tropical and subtropical regions of Brazil. Fruits of physic nut present an oil content of 28% on a dry weight basis. Although the plant has adapted to diverse soil conditions such as low fertility, the correction of soil acidity and the addition of fertilizer are essential for highly productive plants. Thus, the response of the physic nut to different soil phosphorus levels (P) and arbuscular mycorrhizal fungi (AMF) inoculation must be characterized. Hence, the objective of the present study was to evaluate the response of physic nut seedlings to arbuscular mycorrhizal fungi (AMF) inoculation at different levels of soil P. Experiment was carried out in a greenhouse encompassing AMF treatments (inoculation with Gigaspora margarita or Glomus clarum, and the non inoculated controls), and phosphorus treatments (0, 25, 50, 100, 200 and 400 mg kg-1 added to soil). At low soil P levels, arbuscular mycorrhizal fungi inoculation had a significant positive effect on plant growth, shoot and root dry matter content, plant height, number of leaves, total leaf area, leaf area per leaf and the Dickson quality index. The root:shoot ratio and the leaf area ratio were also affected by mycorrhizal inoculation and the level of P addition. Physic nut plants exhibited high mycorrhizal dependency at soil P additions up to 50 mg kg-1.

scholarly journals Field Performance Evaluation of Tea (Camellia sinensis L.) to the Application of Different Organic Wastes under Southwest Nigeria

2020 ◽  
pp. 34-47
Author(s):  
S. A. Adeosun ◽  
A. O. Togun
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Dry Matter ◽  
Block Design ◽  
Stem Diameter ◽  
Dry Matter Content ◽  
Southwest Nigeria ◽  
Number Of Leaves ◽  
Better Than ◽  
Number Of Branches

Aims: To assess the field establishment of two tea cultivars under Cocoa Pod Husk (CPH) and Poultry Manure (PM). Study Design: Randomized complete block design arranged in Split-plots (cultivars as main plots and organic amendments as sub-plots) with four replications. Place and Duration of Study: Cocoa Research Institute of Nigeria stations in Ibadan and Owena, Southwest Nigeria between May 2016 and November 2017 (Rainy and dry seasons of 2016; Rainy season of 2017).  Methodology: Milled CPH and cured PM were applied each at the rates of 150 and 300 kg Nha-1 to established C143 and C318 tea cultivars on the field; unfertilized tea cultivars served as control. Data on number of leaves, number of branches, leaf area, plant height and stem diameter were collected on monthly basis; while dry matter was assessed at 15 months after transplanting. The data were analyzed with ANOVA and correlation at α.05. Results: The C143 performed better than C318 and 150 kg Nha-1 of CPH and PM enhanced the vegetative growth and dry matter of tea better than 300 kg Nha-1 in Ibadan and Owena. Cultivar 143 was significantly (P=0.05) better than cultivar 318 in number of leaves, number of branches and stem diameter in Ibadan; and in number of leaves and leaf area in Owena. CPH at 150 kg Nha-1 increased number of leaves, number of branches, leaf area, plant height and total dry matter by 135.11, 88.19, 346.12, 65.33 and 428.11% at Ibadan; and by 349.09, 245.41, 376.89, 80.89 and 231.49% at Owena, compared to control. On the interaction, tea cultivar 143 that received 150 kg Nha-1 CPH produced significantly (P=0.05) higher number of leaves, leaf area and total dry matter at Ibadan and Owena. Leaf area was positively correlated with number of leaves (r=0.87) in Ibadan and stem diameter (r=0.80) in Owena. Conclusion: CPH at 150 kg Nha-1 enhanced the growth and dry matter content of C143 tea established on the field in Ibadan and Owena, and is therefore recommended for tea cultivation in Southwest of Nigeria.

scholarly journals Influence of Arbuscular Mycorrhizal Fungi (Glomus clarum) and Compost on Early Growth Response of Parkia biglobosa under a Greenhouse Condition

2020 ◽  
pp. 72-79
Author(s):  
F. B. Musa ◽  
O. N. Sulaiman ◽  
V. A. Olayiwola ◽  
F. O. Abiodun ◽  
O. A. Agbo-Adediran
Keyword(s):  
Plant Height ◽  
Mycorrhizal Fungi ◽  
Growth Response ◽  
Arbuscular Mycorrhizal ◽  
Early Growth ◽  
Parkia Biglobosa ◽  
Greenhouse Condition ◽  
Early Growth Response ◽  
Number Of Leaves ◽  
Collar Diameter

Background and Objective: Arbuscular Mycorrhizal Fungi (AMF) plays a role in the structural stability of soil which governs most soil activities. Stable organic manure such as compost may provide a suitable habitat and energy source for mycorrhizal growth, which is also a benefits in view of soil productivity. The impact of a combination of compost and mycorrhizal on plant growth was assessed in this study. Hence, experiment was conducted to investigate the influence of compost and mycorrhizalon the early growth response of Parkia biglobosa under a greenhouse condition. Materials and Methods: In the greenhouse of the Department of Bioscience, Forestry Research Institute of Nigeria. A 2 × 5 factorial experiment in a complete randomized design was conducted; two levels of mycorrhizal (with and without); five levels of compost (10t/ha, 20t/ha, 30t/ha, 40t/ha and no amendments) in two (2) kilogram soil under four (4) replications was set up. Laboratory analyses of soil and organic amendment incorporated in the soil were done. Data on growth variables were taken fortnightly. The data was statistically analyzed and mean were separated using Duncan Multiple Range Test (DMRT). Results: The results showed that, there was significant difference (p<0.05) in the plant height of Parkia biglobosa between mycorrhizal and non-mycorrhizal plants across all weeks after transplanting (WAT),compost application with the interaction of AMF at 40 t/harecorded the highest plant height and number of leaves at 16WAT with 35.14 cm  and 29.75 respectively, which are relatively comparable to other treatments used, the least plant height  and number of leaves were observed when -AMF 0 t/ha ( control) was used as an amendment with 23.00 cm and AMF 0t/ha (15.05) respectively. For collar diameter, all the treatments were comparable to one another except + AMF 30t/ha which produced the lowest collar diameter with mean value of 2.97mm. Conclusion: Based on the description of results above, it can be concluded that: The Inoculation of the AMF and compost significantly affect the early growth performance of Parkia biglobosa, thus providing optimum soil physical conditions for it growth.

scholarly journals Arbuscular Mycorrhizal Fungi Regulate MAPK Pathway Genes Expression and Enhance Drought Tolerance of Populus Simonii × P. Nigra Seedlings

2021 ◽  
Author(s):  
Jing Tao ◽  
Fengxin Dong ◽  
Yihan Wang ◽  
Hui Chen ◽  
Ming Tang
Keyword(s):  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Oxidative Damage ◽  
Mycorrhizal Fungi ◽  
Stomatal Closure ◽  
Expression Patterns ◽  
Soluble Sugar ◽  
Arbuscular Mycorrhizal ◽  
Populus Simonii ◽  
Amf Inoculation

Abstract Background: Arbuscular mycorrhizal fungi (AMF) form a symbiotic relationship with host plants, which can promote plants to absorb more water and nutrients, and thus improve the stress resistance of plants. Our study aimed to investigate the effects of Rhizophagus irregularis on Populus simonii × P. nigra seedlings under drought stress. Results: The experiment was a completely random design with two water conditions (well-watered or drought stress) and two AMF treatments (inoculated with or without R. irregularis). Our results showed that mycorrhizal seedlings performed less oxidative damage and stronger tolerance of drought, which recorded higher photosynthesis and less concentrations of Malondialdehyde (MDA), H2O2, and proline under drought stress versus non-mycorrhizal seedlings. Under drought stress, AMF inoculation reduced soluble sugar concentration in leaves but promoted its accumulation in roots. The superoxide dismutase (SOD) activity in leaves and roots, and catalase (CAT) activity in roots of mycorrhizal seedlings were lower than non-mycorrhizal seedlings, but CAT activity in leaves of mycorrhizal seedlings was higher than non-mycorrhizal seedlings under drought stress. Drought stress and AMF inoculation both induced the expressions of MAPKs of P. simonii × P. nigra, but the expression patterns of MAPKs under four treatments were obviously different.Conclusions: Overall, our results demonstrated that mycorrhizal seedlings had less oxidative damage and stronger tolerance to drought. MAPKs expressions of P. simonii×P. nigra (PsnMAPKs) were induced by drought stress and AMF inoculation, and the expression patterns of PsnMAPKs in response to drought stress were different between mycorrhizal and non-mycorrhizal seedlings. Non-mycorrhizal seedlings may be adapted to drought by up-regulating MAPKs expressions leading to stomatal closure. Drought stress decreased serval PsnMAPKs expressions induced by AMF inoculation, which may be associated with mycorrhizal colonization.

scholarly journals Growth, physiological, and biochemical responses of thyme (Thymus vulgaris L.) to the application of arbuscular mycorrhizal fungi under cadmium stress conditions

2021 ◽  
Vol 49 (3) ◽  
pp. 11924
Author(s):  
Seyed A. HOSSEINI ◽  
Roya ROSTAMI ◽  
Behrooz ESMAIELPOUR ◽  
Alireza ETMINAN ◽  
Ghobad SALIMI
Keyword(s):  
Mycorrhizal Fungi ◽  
Stability Index ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Cadmium Stress ◽  
Thymus Vulgaris ◽  
Cd Stress ◽  
Membrane Stability Index ◽  
Number Of Leaves ◽  
Amf Inoculation

Thyme (Thymus vulgaris L.) is one of the most important medicinal plants used in various pharmaceutical, osmotic, health, and food industries. Arbuscular mycorrhizal fungi (AMF) symbiosis is viewed as one of the several methods to improve growth under heavy metals stress. To investigate the effects of cadmium (Cd) and AMF bio-fertilizers on the growth and morpho-physiological characteristics of thyme, a greenhouse experiment was performed in three replications. Experimental treatments included Cd at three levels 0, 75, and 150 mg/kg of soil and AMF at three levels without inoculation, inoculation with Funneliformis etunicatum, and Funneliformis mosseae. Cadmium stressed plant showed reduced plant height, number of leaves, stem fresh and dry weight, and root fresh and dry weight while AMF inoculation enhanced the increased means of these traits considerably. Inoculation with F. mosseae also ameliorated the Cd stress (150 mg/kg) induced reduction in plant height, number of leaves, and stem and root dry weight by 13.41%, 8.42%, 30.3%, and 22.2%, respectively. Cadmium stress reduced membrane stability index while AMF inoculation enhanced membrane stability index considerably. An increase in soluble carbohydrate and proline content was observed due to Cd stress and AMF inoculation caused a further increase in these two metabolite contents ensuring better growth under Cd stressed conditions. Results indicated that F. mosseae had a higher efficiency in increasing morphological traits and improving physiological characteristics than F. etunicatum. Overall, AMF inoculation, especially F. mosseae significant ameliorative potential for Cd toxicity in thyme plants.

scholarly journals Effects of arbuscular mycorrhizal fungi on early development of persimmon seedlings

2018 ◽  
Vol 30 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Gabriela Silva Machineski ◽  
César Augusto Gotardo Victola ◽  
Carolina Honda ◽  
Oswaldo Machineski ◽  
Maria de Fátima Guimarães ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Leaf Surface ◽  
Arbuscular Mycorrhizal ◽  
Dry Mass ◽  
Sterile Soil ◽  
Vegetative Development ◽  
Leaf Surface Area ◽  
Number Of Leaves ◽  
Amf Inoculation

Abstract The present study evaluated the effects of five species of arbuscular mycorrhizal fungi (AMF) on vegetative development parameters and nutrient uptake of persimmon (Diospyros kaki L.) seedlings. The experiment was carried out in a completely randomized experimental design with six treatments involving AMF inoculation (non-inoculated; Dentiscutata heterogama, Claroideoglomus etunicatum, Rhizophagus clarus, Acaulospora scrobiculata and A. morrowiae), in sterile soil (Oxisol – Dystrophic Red Latosol) under greenhouse conditions. Persimmon seeds were germinated in sterile sand and the seedlings were transplanted to the sterile soil and received AMF inocula. After 360 days, the following vegetative parameters were analyzed: plant height, number of leaves, leaf surface area, stem diameter, shoot and root dry mass, percentage of root colonization and nutrient accumulation in shoot and root biomass. We observed different responses to the AMF species tested. Seedling height and leaf surface area were promoted by inoculation with D. heterogama and A. morrowiae, and these species also promoted a higher percentage of root colonization. Plants inoculated with D. heterogama and C. etunicatum presented a greater number of leaves, but only D. heterogama promoted significant increases in stem diameter. Shoot dry mass was improved by inoculation with D. heterogama, A. morrowiae and C. etunicatum, while the first two species and R. clarus increased root dry mass. Nutrient accumulation in shoot and root biomass was stimulated by AMF inoculation, especially by D. heterogama and A. morrowiae. Therefore, the effects on early vegetative development were more pronounced in the persimmon seedlings inoculated with D. heterogama.

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