Effect of particle size of perlite and its mixture with peat moss on essential oil percent and yield of lemon balm (Melissa officinalis) in hydroponic system

2018 ◽  
Vol 9 (3) ◽  
pp. 39-48
Author(s):  
E. Farrokhi ◽  
A. Samadi ◽  
A. Rahimi ◽  
F. Asadzadeh ◽  
◽  
...  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Abbas Samadi ◽  
Ebrahim Sepehr ◽  
Amir Rahimi ◽  
Sergey Shabala

AbstractMedicinal plants represent a valuable commodity due to beneficial effects of their natural products on human health, prompting a need for finding a way to optimize/increase their production. In this study, a novel growing media with various perlite particle size and its mixture with peat moss was tested for hydroponic-based production of Echinacea purpurea medicinal plant under greenhouse conditions. The plant growth parameters such as plant height, total fresh leave weight, fresh root weight, total biomass, total chlorophyll, leaf area, and essential oil compositions were assessed. Perlite particle size in the growing media was varied from very coarse (more than 2 mm) to very fine (less than 0.5 mm), and the ratio between perlite and peat moss varied from 50:50 v/v to 30:70 v/v. In addition, two nitrate (NO3−) to ammonium (NH4+) ratios (90:10 and 70:30) were tested for each growing media. The medium containing very fine-grade perlite and 50:50 v/v perlite to peat moss ratio was found to be most optimal and beneficial for E. purpurea performance, resulting in maximal plant height, fresh and dry weight, leaf surface area, and chlorophyll content. It was also found that an increase in NO3−/NH4+ ratio caused a significant increase in plant growth parameters and increase the plant essential oil content. The major terpene hydrocarbons found in extract of E. purpurea with the best growth parameters were germacrene D (51%), myrcene (15%), α-pinene (12%), β-caryophyllene (11%), and 1-Pentadecene (4.4%), respectively. The percentages of these terpene hydrocarbons were increased by increasing of NO3−/NH4+ ratio. It can be concluded that decreasing the perlite particle size and increasing the NO3−/NH4+ ratio increased the plant growth parameters and essential oil compositions in E. purpurea.


2017 ◽  
Vol 29 (4) ◽  
pp. 351-356 ◽  
Author(s):  
Katarzyna Seidler-Łożykowska ◽  
Renata Zawirska-Wojtasiak ◽  
Elżbieta Wojtowicz ◽  
Jan Bocianowski

2012 ◽  
Vol 22 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Ana Carolina B. Sodré ◽  
José Magno Q. Luz ◽  
Lenita L. Haber ◽  
Marcia O. M. Marques ◽  
Carlos R. Rodrigues ◽  
...  

2021 ◽  
Vol 5 (2) ◽  
pp. 313-319
Author(s):  
Ahmet YENKALAYCI ◽  
Mahmut GUNES ◽  
Kemal GUL

This study was carried out to determine yield and quality characteristics of Lemon balm (M. officinalis L.) in in 7 districts (7 locations) under Kayseri ecological conditions for 3 years in 2015-2017.  In this research, herbage yield, essential oil content, essential oil yield and components were determined. Dry herbage yields varied between 5250-6100 kg ha-1, essential oil contents varied between 0.07-0.24%, and essential oil yields as 4.2 lt ha-1. The main essential oil components were gereniol (1.90-20.77%), nerylacetate (1.13-34.63%), linalool (4.04-25.34%), neral (5.10-12.96%), caryophylleneoxide (0.78-15.17%), geranial (1.20-14.19%), β–caryophyllene (8.50-12.93%). In terms of herbage yield and essential oil quality, Yahyalı and Kocasinan districts, had favorable environmental conditions for lemon balm cultivation. 


2020 ◽  
Author(s):  
Mariana Daniela Berechet ◽  
Demetra Simion ◽  
Maria Stanca ◽  
Cosmin Andrei Alexe ◽  
Rodica Roxana Constantinescu ◽  
...  

Lemon balm (Melissa officinalis L.) belongs to the Lamiaceae family. Essential oil extracted from the aerial parts of lemon balm has been investigated for the protection of fruits during storage, as insecticidal, as well as in medicine, due to its bioactive properties. In this paper, the composition and identification of components from Melissa officinalis L. essential oil were determined by gas chromatography coupled with mass spectrophotometry (GC/MS) analysis. Total phenol content (TPC) and the scavenging activity towards 2,2-diphenyl-1-picrylhydrazyl (DPPH·) and 2,2′-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid (ABTS+·) free radicals were evaluated by UV-VIS spectrometry. Antibacterial activities were carried out against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. Seventeen bioactive compounds were found as constituents of Melissa officinalis L. essential oil, among which o-cymene (19.735%), dehydro-p-cymene (17.180%), and limonene (11.589%) were found as the major components. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR) provided a confirmation for the chemical components of lemon balm essential oil identified by GC/MS. The values recorded for TPC and antioxidant activity were as follows: 54.72 mg GAE/g dry substance, 28.53% for DPPH, and 46.17% for ABTS assays, respectively. 100 µL lemon balm essential oil proved total antibacterial activity against the tested microorganisms. The results showed that the Melissa officinalis L. may be a good candidate as plant-derived antioxidant and antibacterial agent for medical footwear, wound dressings and other medical applications.


2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Tayebeh Ahmadi ◽  
Leila Shabani ◽  
Mohammad R. Sabzalian

Abstract Background Nowadays, light-emitting diodes (LEDs) as a new lighting technology, have been emerged as an alternative source of light for plants due to their wavelength specificity, the narrow width of their bands, small size, solid structure, long lifetime, and low heat generation. Here we investigated the effect of different LED light sources on the essential oil components and antioxidant activity of Melissa officinalis. Two genotypes of lemon balm (Ilam and Isfahan) were subjected to four artificial light treatments, including white, red, blue, red + blue LEDs, and greenhouse light as natural lighting. Results The LED lights significantly increased shoot fresh and dry weights and leaf number in the two genotypes as compared to greenhouse condition. The results showed that the content and composition of essential oil in the two genotypes were variable under different light treatments and the total amount of compounds in the Ilam genotype was higher than the other genotype. The results of analysis of the essential oil by GC/MS indicated that the highest amount of monoterpenes in the genotypes was related to citronellal under red + blue LED lamps (15.3 and 17.2% in Ilam and Isfahan genotypes, respectively) but blue, white, and greenhouse condition had the most effect on sesquiterpenes content in both genotypes. The results showed that the observed variation between the two genotypes in the essentials oil composition was related to the relative percentage of the constituents and not to the appearance or lack of a specific component. Red + blue lighting also provided the highest radical scavenging activity in both genotypes (80.77 and 82.09% for Ilam and Isfahan genotypes, respectively). Based on principal component analyses (PCA), three main groups were identified regarding genotypes and all light treatments. Conclusions Overall, results indicated that the essentials oil composition of two genotypes of lemon balm was affected both qualitatively and quantitatively by different LED light sources; hence, LED lights might be used to improve monoterpenes, sesquiterpenes, and antioxidant activity in the selected genotypes.


2017 ◽  
Vol 65 (3) ◽  
pp. 745-757 ◽  
Author(s):  
J. Kittler ◽  
H. Krüger ◽  
U. Lohwasser ◽  
D. Ulrich ◽  
B. Zeiger ◽  
...  

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