scholarly journals Immunocytochemical Detection of Kisspeptin Receptor and Its Association with Motility of Buffalo Bull (Bubalus bubalis) Spermatozoa

2020 ◽  
Vol 40 (02) ◽  
pp. 244-248
Author(s):  
Akhtar Hussain
Keyword(s):  
Bubalus Bubalis ◽  
Immunocytochemical Detection

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

NeuN As a Neuronal Nuclear Antigen and Neuron Differentiation Marker

Acta Naturae ◽  
2015 ◽  
Vol 7 (2) ◽  
pp. 42-47 ◽  
Author(s):  
V. V. Gusel’nikova ◽  
D. E. Korzhevskiy
Keyword(s):  
Experimental Data ◽  
Intracellular Localization ◽  
Nuclear Antigen ◽  
Structure And Properties ◽  
Immunocytochemical Detection ◽  
Perinuclear Cytoplasm ◽  
Diagnosis Of Cancer ◽  
Morphological Diagnosis ◽  
The Central Nervous System ◽  
Differentiation Marker

The NeuN protein is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. Monoclonal antibodies to the NeuN protein have been actively used in the immunohistochemical research of neuronal differentiation to assess the functional state of neurons in norm and pathology for more than 20 years. Recently, NeuN antibodies have begun to be applied in the differential morphological diagnosis of cancer. However, the structure of the protein, which can be revealed by antibodies to NeuN, remained unknown until recently, and the functions of the protein are still not fully clear. In the present mini-review, data on NeuN accumulated so far are summarized and analyzed. Data on the structure and properties of the protein, its isoforms, intracellular localization, and hypothesized functions are reported. The application field of immunocytochemical detection of NeuN in scientific and clinical studies, as well as the difficulties in the interpretation of the obtained experimental data and their possible causes, is described in details.

BÚFALO DE AGUA (Bubalus bubalis): UN ACERCAMIENTO AL MANEJO SUSTENTABLE EN EL SUR DE VERACRUZ, MÉXICO

2018 ◽  
Vol 11 (10) ◽  
Author(s):  
D. Vázquez-Luna
Keyword(s):  
Bubalus Bubalis ◽  
El Sistema

Objetivo: Analizar los principales indicadores asociados al manejo cultural del sistema búfalo en el sur de Veracruz, México (municipios de Acayucan, Sayula, Minatitlán, Soconusco, Hidalgotitlán, San JuanEvangelista, Las Choapas, Oluta, Jesús Carranza y Valle de Uxpanapan), para el desarrollo sustentable.Diseño/metodología/aproximación: El método de análisis de la presente investigación fue de tipo cuantitativo y el instrumento fue el cuestionario, los resultados se procesaron en el software estadísticoDYANE ver. 4.0 y los indicadores de sustentabilidad fueron desarrollados a partir de tres ejes transversales (social, económico y productivo).Resultados: Los resultados muestran valores elevados en los indicadores económicos, debido a que los productores perciben que la actividad ganadera con búfalos, es un negocio rentable.Limitaciones del estudio/implicaciones: En el eje productivo correspondió al patrón socio-cultural de la ganadería en el trópico, el cual se perpetúa generación tras generación, por el sistema social en elcual está inserto; sin embargo, se recomienda desarrollar el potencial en los ejes social, productivo y económico hacia la sustentabilidad del sistema búfalo.Hallazgos/conclusiones: Destacan indicadores del eje económico, debido a la percepción de rentabilidad; pero falta realizar actividades para el desarrollo sustentable en los ejes económico, social y productivo.

SERUM BIOCHEMICAL CHANGES IN KETOTIC BUFFALOES ( BUBALUS BUBALIS)

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Usha Choudhary ◽  
Meenaxi Sareen ◽  
Anil Moolchandani
Keyword(s):  
Serum Calcium ◽  
Bubalus Bubalis ◽  
Biochemical Changes ◽  
Blood Analysis ◽  
Serum Phosphorus ◽  
Serum Minerals ◽  
Calcium Magnesium ◽  
Serum Biochemical ◽  
Calcium And Magnesium

The present study was carried out to evaluate the variations in serum minerals ( viz. calcium, magnesium and phosphorus) and transaminases in ketotic buffaloes. Results of blood analysis revealed a significant (P<0.05) reduction in serum phosphorus in ketotic buffaloes whereas nonsignificant (P>0.05) difference occur in serum calcium and magnesium in ketotic buffaloes as compared to control. At the same time significant increase in transaminases GPT, GOT and GGT have been observed .

scholarly journals Haplotype diversity in the mitochondrial genome of the Egyptian river buffalo (Bubalus bubalis)

2021 ◽  
Vol 6 (1) ◽  
pp. 145-147
Author(s):  
Nouran Adel Youssef ◽  
Manon Curaudeau ◽  
Soheir Mohamed El Nahas ◽  
Amal Ahmed Mohamed Hassan ◽  
Alexandre Hassanin
Keyword(s):  
Mitochondrial Genome ◽  
Haplotype Diversity ◽  
Bubalus Bubalis ◽  
River Buffalo

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

Hematology and biochemistry of buffalo (Bubalus bubalis): influence of sex and age on reference values

2021 ◽  
Vol 53 (2) ◽  
Author(s):  
Thais Bastos Rocha ◽  
Ruan da Cruz Paulino ◽  
Diego Moraes Soares ◽  
Wendel Adelino Policarpo ◽  
Ednaldo da Silva Filho ◽  
...  
Keyword(s):  
Reference Values ◽  
Bubalus Bubalis
Sign in / Sign up

Export Citation Format

Share Document