EXPRESSION OF β-DEFENSIN-1 IN RAT COLON TISSUE

Abstract The aim of this experiment was to investigate the infl uence of inulin administration on chemopreventive and inflammatory markers in dimethylhydrazine induced colorectal cancer development in rats. A group of 30 Sprague-Dawley rats was divided into a control group (CG), a group with dimethylhydrazine (DMH), and a group given dimethylhydrazine combined with the prebiotic (DMH+PRE). Dimethylhydrazine injection significantly (p<0.001) elevated the immunoreactivity chemopreventive markers COX-2, NFκB, iNOS, elevated serum and jejunal mucosa levels of proinfl ammatory cytokine IL-2, and decreased serum and jejunal mucosa levels of regulatory cytokine IL-10. Inulin diet intervention significantly suppressed immunoreactivity of COX- 2, NFκB, iNOS positive cells in the tunica mucosae and tela submucosae of rat colon tissue, increased levels of IL-2 and decreased levels of IL-10. By determining the chemopreventive markers COX-2, iNOS and NFkB, which can be characterized as inflammatory markers, we confirmed the presence of inflammation in the colon as the number of COX-2, NFkB and iNOS immunoreactive cells was significantly higher after DMH application than in the control group. These findings indicate that dietary intake of inulin suppressed the expression of the observed markers, which play an important role in carcinogenesis and inflammation, which predispose the use of inulin in the prevention or treatment of human chronic diseases and its use as a nutritional supplement in veterinary medicine.

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Bone Marrow Cell Therapy on 1,2-Dimethylhydrazine (DMH)-Induced Colon Cancer in Rats

Cellular Physiology and Biochemistry ◽

10.1159/000487349 ◽

2018 ◽

Vol 45 (3) ◽

pp. 1072-1083 ◽

Cited By ~ 9

Author(s):

Manal F. El-Khadragy ◽

Heba M. Nabil ◽

Basmaa N. Hassan ◽

Amany A. Tohamy ◽

Hanaa F. Waaer ◽

...

Keyword(s):

Gene Expression ◽

Bone Marrow ◽

Colon Cancer ◽

Bone Marrow Cells ◽

Histopathological Examination ◽

Aberrant Crypt Foci ◽

Rat Colon ◽

Cytokeratin 20 ◽

Colon Tissue ◽

Mdr 1

Background/Aims: Stem cell based therapies are being under focus due to their possible role in treatment of various tumors. Bone marrow stem cells believed to have anticancer potential and are preferred for their activities by stimulating the immune system, migration to the site of tumor and ability for inducting apoptosis in cancer cells. The current study was aimed to investigate the tumor suppressive effects of bone marrow cells (BMCs) in 1,2-dimethylhydrazine (DMH)-induced colon cancer in rats. Methods: The rats were randomly allocated into four groups: control, BMCs alone, DMH alone and BMCs with DMH. BMCs were injected intrarectally while DMH was injected subcutaneously at 20 mg/kg body weight once a week for 15 weeks. Histopathological examination and gene expression of survivin, β-catenin and multidrug resistance-1 (MDR-1) by real-time reverse transcription-polymerase chain reaction (RT-PCR) in rat colon tissues. This is in addition to oxidative stress markers in colon were performed across all groups. Results: The presence of aberrant crypt foci was reordered once histopathological examination of colon tissue from rats which received DMH alone. Administration of BMCs into rats starting from zero-day of DMH injection improved the histopathological picture which showed a clear improvement in mucosal layer, few inflammatory cells infiltration periglandular and in the lamina propria. Gene expression in rat colon tissue demonstrated that BMCs down-regulated survivin, β-catenin, MDR-1 and cytokeratin 20 genes expression in colon tissues after colon cancer induction. Amelioration of the colon status after administration of MSCs has been evidenced by a major reduction of lipid peroxidation, nitric oxide, and increasing of glutathione content and superoxide dismutase along with catalase activities. Conclusion: Our findings demonstrated that BMCs have tumor suppressive effects in DMH-induced colon cancer as evidenced by down-regulation of survivin, β-catenin, and MDR-1 genes and enhancing the antioxidant activity.

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Label-free Raman spectroscopic imaging to extract morphological and chemical information from a formalin-fixed, paraffin-embedded rat colon tissue section

International Journal of Experimental Pathology ◽

10.1111/iep.12194 ◽

2016 ◽

Vol 97 (4) ◽

pp. 337-350 ◽

Cited By ~ 15

Author(s):

Riana Gaifulina ◽

Andrew Thomas Maher ◽

Catherine Kendall ◽

James Nelson ◽

Manuel Rodriguez-Justo ◽

...

Keyword(s):

Tissue Section ◽

Spectroscopic Imaging ◽

Rat Colon ◽

Chemical Information ◽

Label Free ◽

Colon Tissue ◽

Raman Spectroscopic ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Finger Citron Extract Ameliorates Glycolipid Metabolism and Inflammation by Regulating GLP-1 Secretion via TGR5 Receptors in Obese Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/6623379 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Yujiao Yang ◽

Aofei Tian ◽

Zhen Wu ◽

Yao Wei ◽

Xuguang Hu ◽

...

Keyword(s):

Endocrine Cells ◽

Liver Lipid ◽

Chinese Herbs ◽

Rat Colon ◽

Enzyme Linked Immunosorbent Assay ◽

Colon Tissue ◽

Glycolipid Metabolism ◽

Obese Rats ◽

Intestinal Endocrine Cells

Finger citron (FC) is one of many traditional Chinese herbs that have been used to treat obesity. The aim of this study was to elucidate the pharmacological effects and mechanisms of FC on obese rats. Rats were fed with a high-fat diet as a model of obesity and treated with FC at three different dosages for 6 weeks. Pathology in liver tissue was observed. Glucose levels, lipids levels, and inflammatory indicators in serum were evaluated by enzyme‐linked immunosorbent assay. Furthermore, the expression of G protein-coupled receptor 5 (TGR5) pathway genes in rat colon tissue was detected by reverse transcription-polymerase chain reaction analysis (RT-PCR). Our result revealed that FC alleviates obesity by reducing body weight (BW) and waist circumference, managing inflammation and improving glycolipid metabolism, liver function, and liver lipid peroxidation in vivo. In addition, the mechanism of FC on obesity is possibly the stimulation of glucagon-like peptide-1 (GLP-1) secretion by activating the TGR5 pathway in intestinal endocrine cells. Our studies highlight the obesity reduction effects of FC and one of the mechanisms may be the activation of the TGR5 pathway in intestinal endocrine cells.

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