scholarly journals SEMEN pH;

2012 ◽  
Vol 19 (02) ◽  
pp. 234-241
Author(s):  
HENRY. A. MWAKYOMA ◽  
ERICK. P. MAGOROSA

Background: Male infertility is a frequent reproductive health problem in the world. It is usually related to abnormal spermproduction or function and these abnormalities can occur anywhere in the production of sperm including hormonal regulation, storage andtransport of sperm. Various factors are known to be responsible for seminal fluid abnormalities. Usually the first step in evaluating for maleinfertility is semen analysis. Setting: The study was conducted at Muhimbili National Hospital mainly in the department of Pathology laboratory,cytology unit. Study design: The study was a hospital based cross-sectional type. Objective: To determine semen pH in patients presenting withinfertility complains and relate how pH of seminal fluid and other semen parameters influence each other. Material and methods: Beforesemen collection, patients were instructed to abstain from sexual intercourse for a minimum of 48 hours to 7 days and collect semen bymasturbation and transport it to the laboratory through shirt pocket. Semen was to reach the laboratory for examination in not more than onehour from time of collection. Semen was examined macroscopically for volume, colour, viscosity and pH by using a pH meter (Consort C830)followed by microscopic examination which included motility of spermatozoa and sperm count by using Neuber counting chamber. The smearwas made on glass slides, fixed in 95% ethyl alcohol for 30 minutes then stained by using Papanicolaou’s staining technique and then analyzedmicroscopically for morphological examination. Results: In the analysis of the influence of semen parameters on semen pH, there wasdecrease in seminal fluid pH with age whereby as age increased the pH of seminal fluid decreased.. The general trend observed was that thepH of seminal fluid tended to decrease with an increase in the days of abstinence. pH tended to decrease with an increase of seminal fluidvolume. The pH of seminal fluid also increased with an increase in viscosity (Hyperviscosity >Hypoviscosity). The pH of seminal fluid in patientswith less than 50% forward progressive movement of spermatozoa was higher when compared to those with more than 50% forwardprogressive movement. pH had an influence on the motility of spermatozoa. Conclusions and recommendation: pH and other parameterstended to have an influence each other during seminal analysis in our study. There is a variation of pH in different parts of the World according tothe studies done. It is recommended that pH should be included during seminal analysis because our study has shown that it affects most of theseminal fluid parameters in and contribute to the problem of infertility.

1969 ◽  
Vol 3 (2) ◽  
pp. 303-307
Author(s):  
NAIK ZADA ◽  
SHAFI ULLAH KHAN ◽  
RIAZ AHMAD KHAN

OBJECTIVE: To determine the frequency of abnormal semen parameters among patients presenting withvaricocele1METHODS:It was a descriptive cross-sectional study conducted at the Department of Urology Institute ofkidney diseases Hayat Abad Medical Complex Peshawar and Cenna hospital Saidu Sharif Swat. The studywas carried out on 139 human subjects with clinical evidence of varicocele between age range of 15-45years.The diagnosis of varicocele was based on palpable and/or visible scrotal lump of testicular veins(pampiniform plexus) and was diagnosed on the basis of clinical examination. Semen analysis was carriedout in all these patients and information wascollected on pre designed proforma.RESULTS:The study included a total of 139 patients with varicocele. The mean age of patient was 30 years(15-45) among the patients having symptoms of varicocele. The Mean ±SD for duration of varicocelesymptoms was 9.32 ± 9.70 months. 6.5% (n=9) patients were having azoospermia and 20.1% (n=28)patients had oligozoospermia.CONCLUSION: Patients with varicocele have poor seminal parameters in terms of sperm count i.e.oligozoospermia and azoospermia responsible for male factor infertility in majority ofcases.KEYWORDS:varicocele;seminal parameters;sperm count, infertility


2022 ◽  
Vol 71 (6) ◽  
pp. 2224-27
Author(s):  
Aysha Khan ◽  
Zunera Shahbaz ◽  
Shagufta Yousaf ◽  
Abeera Ahmed ◽  
Fatima Sana ◽  
...  

Objective: To study the patterns and distribution of various abnormal semen parameters in infertile males. Study Design: Cross sectional study. Place and Duration of Study: Department of Pathology, Combined Military Hospital, Karachi, from Nov 2019 to Oct 2020. Methodology: The study included 364 patients who presented with primary and secondary infertility. Consecutive convenient sampling was done. Semen analysis was performed using World Health Organization latest guidelines. Samples were categorized as normospermia, azoospermia, oligospermia, asthenozoospermia and necrospermia. Results: The study comprised of 364 samples of infertile males. Normal sperm count was observed in 317 (87%) males, azoospermia in 28 (7.6%) and oligospermia in 19 (5.2%) males. Low ejaculated volume and higher non-motile sperms were noted in oligospermia samples in comparison with normospermia samples. Asthenozoospermia was observed in 102 (28%) and oligoasthenospermia was noted in 15 (4.1%) samples. Conclusion: Good quality semen analysis is a corner stone to diagnose the cause of male infertility.Sperm concentration and motility are the important markers of normal male reproductive system and are related to each other.


Author(s):  
Surekha Bhalekar ◽  
Shweta Ganorkar ◽  
Hemant Bhalekar ◽  
Prakash Roplekar

Background: Although semen analysis is routinely used to evaluate male partner in infertile couples, infertility and problems of impaired fecundity have been a concern through ages and is also a significant clinical problem today, which affects 8-12% of couples worldwide. Aim of the study was to study different semen parameters in male factor infertility (MFI) and thus increasing the awareness regarding same.Methods: This is cross sectional study conducted between period of September 2016 to December 2018. Semen of 150 patients were studied and results were analysed as per recent WHO (2010) criteria.Results: The present study included 150 patients whose age ranged from 24 to 51 years. Patients were divided into different age groups and sperm count was studied in each group. Abnormal sperm morphology was studied with respect to sperm head, neck, tail defects and combined defects. Sperm deformity index (SDI) and Teratozoospermic index (TZI) were calculated. Other parameters including semen volume, pH, liquefaction time, sperm vitality and motility were also studied which showed significant variations. Conclusions: Although semen analysis is first and most informative investigation for evaluation of male factor infertility, studying individual semen parameters and sperm function and increasing its awareness in general population especially in developing countries is equally important. Besides, it is necessary to acknowledge its limitation with respect to collection, processing, evaluation and biological variation of samples. Also, a normal semen analysis may not prove successful fertility potential of an individual.


2021 ◽  
pp. 1-5
Author(s):  
Dalal Salih Abdel-aziz ◽  
◽  
Mohammed Ahmed Ibrahim Ahmed ◽  
Esam Eldin Osman Elghazali ◽  
Areeg Salih Abdel Aziz Ahmed ◽  
...  

Background: For a long time, bacterial infection of the male genital tract was thought to be one of the leading causes of male infertility. Various clinical studies have identified Leucocytospermia as a proxy marker for these infections, although other causes of inflammation may also play a role. Objective: The study was conducted at the Dermatology Teaching Hospital in Port Sudan with the aim of determining the impact of leucocytospermia on semen parameters and defining the microbial etiology among infertile males. Methods: Between September 2019 and February 2020, a descriptive, cross-sectional, hospital-based investigation was applied. After meeting the study requirements, 140 patients were randomly selected; patient information was collected via a closed-ended questionnaire after patients provided their authorization. Results: A total of 140 male infertility patients were evaluated. The mean age of respondents was 43.5 + 2.6 years old, 61.4% of the patients had infertility for 1-5 years, 55.7% of the patients had secondary infertility, 32.1% of the patients demonstrated leucocytospermia on their semen analysis. Semen analysis results showed that 37.8 of the leucospermic patients’ sperm count was <15 X 106. In 73.3% of the patients, the motile sperms were < 40%, and normal morphology was less than 4% in 46.7% of the patients. The analysis showed mixed infection by both gram positive and negative bacteria are common (42.2%). Conclusion: According to the findings, there is an association between leucocytospermia and male infertility.


Author(s):  
Haytham M. Nasser ◽  
Ahmed Hussein ◽  
Gad M. Behairy ◽  
Mostafa Abdo

Abstract Background Varicocele is an abnormally dilated pampiniform plexus of the veins within the spermatic cord and is considered the most common correctable cause of male factor infertility. Many approaches are described for treatment either surgical (tradition inguinal, subinguinal, and laparoscopic) or non-surgical percutaneous embolization. During the period from August 2017 to December 2018, we prospectively analyzed the preoperative and post-operative alteration of semen parameters (at 3 and 9 months) of the data collected from 63 patients with clinically evident varicocele referred to our tertiary hospital. Patients were divided into two groups: group 1, thirty-three patients who underwent subinguinal microsurgical ligation, and group 2, thirty patients who underwent percutaneous embolization. Results Sixty-three patients enrolled in this study were divided in two groups: group 1, patients who underwent surgery, and group 2, patients who underwent embolization; the mean age is 24.6 ± 1.27 years in group 1 and 23.7 ± 2 years in group 2; there was no statistically significant difference between the two groups as regards BMI, diabetes, hypertension, and smoking. Bilaterality was present in 15.2% of group 1 patients and 10% in group 2 patients (P value 0.06). Most of the patients were classified as grades 2 and 3 with no statistical significance regarding severity of the disease. Preoperative semen parameters for patients including sperm count, motility, and abnormal forms showed no statistically significant difference between the two groups. Post-intervention semen analysis was done twice during follow-up after 3 months and 9 months from the date of intervention. After 3 months, the semen parameters were improved in both groups in spite of the higher sperm count in group 2 but with no statistical significance. After 9 months follow-up, semen analysis showed persistent increase in sperm mobility in group 1 patients in comparison to group 2 patients. Both groups had better improvement in count of normal form with no statistical significant change. Conclusion Improvement of semen parameters while treating primary varicocele by either subinguinal microsurgery approach or percutaneous embolization shows equivalent outcomes.


Author(s):  
Lidia Caporossi ◽  
Alessandra Alteri ◽  
Giovanni Campo ◽  
Enrico Paci ◽  
Giovanna Tranfo ◽  
...  

Among the possible risk factors for male reproduction, exposure to phthalates and alkylphenols is widely documented. This study evaluated the possible association between chemical exposure and the quality of the seminal fluid of 105 subjects in a fertility clinic. The urinary levels of seven phthalate metabolites (monoethylphthalate, MEP; monobenzylphthalate, MBzP; mono n-butylphthalate, MnBP; mono-(2-ethylhexyl) phthalate, MEHP; mono(2-ethyl-5-hydroxyhexyl) phthalate, MEHHP; mono-n-octylphthalate, MnOP; mono-isononylphthalate, MiNP) and bisphenol A (BPA), were analysed by high performance liquid chromatography/tandem mass spectrometry HPLC/MS/MS. The regression analysis showed that the semen volume was positively associated with MnBP, MnOP and BPA levels while was negatively associated with MiNP levels. The sperm concentration had a significant inverse relationship with MEP levels. A negative association was found between the use of plastic containers for food storage (p = 0.037) and semen volume (3.06 vs. 2.30 mL as average values, never vs daily). A significant positive correlation emerged (p < 0.005) between the consumption of canned food and the levels of BPA (2.81 vs. 0.14 µg/g creat as average values, daily vs. never) and between the use of perfumes and levels of MEP (389.86 vs. 48.68 µg/g creat, as average values, daily vs. never). No further statistically significant associations were found, even considering the working activity. Some evidence emerged about the possible link between exposure and seminal fluid quality: further case/control or prospective studies will allow us to confirm this causality hypothesis.


Author(s):  
W. N. Li ◽  
M. M. Jia ◽  
Y. Q. Peng ◽  
R. Ding ◽  
L. Q. Fan ◽  
...  

Abstract Background The aim of this study was to provide information on the semen quality pattern of infertile men and age thresholds for semen parameters in China. Methods This was a retrospective cross-sectional study investigating 71,623 infertile men from the Reproductive and Genetic Hospital of CITIC Xiangya in Hunan, China, from 2011 to 2017. The Kruskal-Wallis test, Mann-Kendall test, linear regression model and joinpoint regression were used. Results Although erratic changes were observed in the median semen parameters (sperm concentration 40.1–52.1 × 106/ml, total sperm count 117.8–153.1 × 106, sperm progressive motility 33.4–38.1%) during the 7 years of observation, no significant decrease in semen quality was found, and 47.88% of infertile men showed normal semen parameters according to the World Health Organization (WHO) criteria. According to the joinpoint regression analysis, sperm progressive motility appeared to decrease earlier than the sperm concentration and total sperm count (at 28, 58, and 42 years of age, respectively). Conclusions There is no evidence of a deterioration in semen quality among infertile men in Hunan, China. Semen parameters decreased with increasing age, with turning points noted at different ages. Semen parameters are not absolute evidence for the assessment of male fertility potential. Therefore, we believe that, among semen parameters, the sperm concentration is the best predictor of fertility for ART, followed by motility. Decreased sperm motility may affect natural pregnancy, but it is not necessary for successful IVF.


1999 ◽  
Vol 27 (01) ◽  
pp. 123-128 ◽  
Author(s):  
Jung-Chou Chen ◽  
Ming-Xiong Xu ◽  
Leih-Der Chen ◽  
Yan-Nian Chen ◽  
Tsan Hung Chiu

The purpose of this study was to investigate the effects of Panax notoginseng extracts on inferior sperm motility in vitro. Semen samples were collected from 23 patients with sperm motility between 20% and 40%. The sperm count was over 20 × 106/ml in accordance with the World Health Organization standard. 1.0 mg/ml and 2.0 mg/ml of Panax notoginseng extracts including aqueous extract, n-butanol extract, and polysaccharide fraction on sperm motility and progression were evaluated by computer assisted semen analysis. The results demonstrated that sperm motility as well as progression on inferior sperm motility were enhanced at 1 hour and 2 hours after incubation with all three types of extracts.


2018 ◽  
Vol 25 (07) ◽  
pp. 1024-1028
Author(s):  
Jahangir Sultan ◽  
Shafqat Husnain Khan ◽  
Munir Ahmed

Objective: The objective of this study was to study the semen parameters and its relation to hormonal status in infertile men. Study Design: Cross sectional study. Period: March 2015 to April 2017. Setting: Jinnah Hospital Fertility Centre; Department of Obstetrics and Gynecology, Lahore. Method: A total of 70 infertile men were included in this study. Semen samples for semen analysis and serum for hormonal analysis were obtained and further tests were performed. Results: The results of our study show that 28.57% were Normospermic, 18.56% were azoospermic and 52.85% were Oligospermic. In Oligospermic and azoospermic patients significant low levels of testosterone and prolactin were noticed. The results indicate that along with semen parameters hormonal levels should also be assessed to diagnose the underlying cause of infertility. Conclusion: It is concluded from our study that both quality of semen and hormonal levels cause infertility in men. Therefore the patients should be properly investigated and should be treated accordingly to the underlying abnormality. This is a regional study and further studies should be conducted to have a conclusive evidence at national level.


2021 ◽  
Author(s):  
Berhe Tesfai ◽  
Fitsum Kibreab ◽  
Hailemichael Gebremariam ◽  
Liwam Abraham

Abstract Background Semen analysis is the first step to identify male factor infertility. World Health Organization estimates that male factor accounts for 50% of couple sub-fertility. The objective of this study was to determine the prevalence and patterns of male factor infertility based on semen analysis in patients visiting Massawa Hospital with infertility complaints. Methods It was a retrospective, descriptive cross sectional hospital based type of study with a census sampling method. Patient’s medical records and hospital laboratory register were used to retrieve semen analysis results of patients from June 2018 to June 2020. Ethical approval was obtained from the Ministry of Health Research and Ethical Review Committee. Confidentiality of patients records kept was secured and consent was obtained from study participants to use their data. Results were presented in frequency, tables and p value < 0.05 was considered significant. Results A total of 112 patients data was analyzed in the study with 49.1% were aged between 20 to 30 years. The prevalence of male factor infertility in these patients was found to be 42% and 79.5% of them had primary type of infertility. Of the study participants; 63 (56.3%), 72 (64.3%) and 70 (62.5%) had sperm count < 15 million sperms/ml, sperm motility < 40 % and morphology of < 60% respectively. One tenth, 15 (13.4%) of the patients had a semen volume of < 1.5ml/ejaculate, out of which 13(86.7%) had primary type of infertility. Moreover; 72 (64.3%) patients had total sperm count/ejaculate of < 39 million and 59 (82%) of these had primary type of infertility. In addition; 50.8% and 50% of patients aged 20 to 30 years had a sperm count < 15 million/ml and sperm motility of < 40% respectively. Conclusion The prevalence of male factor infertility was slightly higher and was dominated with primary infertility. Most patients had lower sperm count, sperm motility but higher semen volume, and majority of the patients with abnormal sperm results were aged between 20 to 30 years. Further prospective researches to determine the risk factors for male infertility and introducing assisted type of fertility in Eritrea are highly recommended.


Sign in / Sign up

Export Citation Format

Share Document