scholarly journals Molecular Biology of Chili Pepper Anthocyanin Biosynthesis

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
César Aza-González ◽  
Héctor Gordon Núñez-Palenius ◽  
Neftalí Ochoa-Alejo
Keyword(s):  
Molecular Biology ◽  
Biosynthetic Pathway ◽  
Genetic Manipulation ◽  
Current Knowledge ◽  
Anthocyanin Biosynthesis ◽  
Chili Pepper ◽  
Horticultural Crop ◽  
Research Areas ◽  
Capsicum Spp ◽  
Chili Peppers

Chili pepper (<em>Capsicum spp.</em>) is an important horticultural crop worldwide. Chili pepper fruits from different <em>Capsicum </em>species have been highly consumed in Mexico since pre-Columbian times. Some <em>Capsicum</em> species synthesize and accumulate anthocyanins in different tissues and organs. Although the anthocyanin biosynthetic pathway has been established for different plant species, very few studies on anthocyanin chemistry, biochemistry and molecular biology of these pigments produced by chili peppers have been reported. In this review we describe the information on the type of anthocyanins synthesized and accumulated in chili pepper, and also on the molecular biology of the biosynthetic pathway. Additionally, we discuss the applications of current knowledge for the genetic manipulation, through genetic engineering, of this trait, and also the future anthocyanin-related research areas in <em>Capsicum.</em>

scholarly journals Transcriptional Regulation of Ripening in Chili Pepper Fruits (Capsicum spp.)

2021 ◽  
Vol 22 (22) ◽  
pp. 12151
Author(s):  
Maria Guadalupe Villa-Rivera ◽  
Neftalí Ochoa-Alejo
Keyword(s):  
Transcription Factors ◽  
Nutritional Value ◽  
Current Knowledge ◽  
Chili Pepper ◽  
Transcriptional Level ◽  
Horticultural Crop ◽  
Ripening Process ◽  
Capsicum Spp ◽  
Chili Peppers ◽  
Visual Changes

Chili peppers represent a very important horticultural crop that is cultivated and commercialized worldwide. The ripening process makes the fruit palatable, desirable, and attractive, thus increasing its quality and nutritional value. This process includes visual changes, such as fruit coloration, flavor, aroma, and texture. Fruit ripening involves a sequence of physiological, biochemical, and molecular changes that must be finely regulated at the transcriptional level. In this review, we integrate current knowledge about the transcription factors involved in the regulation of different stages of the chili pepper ripening process.

scholarly journals The Flavonoid Biosynthesis Network in Plants

2021 ◽  
Vol 22 (23) ◽  
pp. 12824
Author(s):  
Weixin Liu ◽  
Yi Feng ◽  
Suhang Yu ◽  
Zhengqi Fan ◽  
Xinlei Li ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Current Knowledge ◽  
Anthocyanin Biosynthesis ◽  
Basic Structure ◽  
Ring Structure ◽  
Flavonoid Biosynthesis ◽  
Comprehensive Overview ◽  
Flavonoid Biosynthetic Pathway ◽  
Intermediate Metabolites ◽  
Important Intermediate

Flavonoids are an important class of secondary metabolites widely found in plants, contributing to plant growth and development and having prominent applications in food and medicine. The biosynthesis of flavonoids has long been the focus of intense research in plant biology. Flavonoids are derived from the phenylpropanoid metabolic pathway, and have a basic structure that comprises a C15 benzene ring structure of C6-C3-C6. Over recent decades, a considerable number of studies have been directed at elucidating the mechanisms involved in flavonoid biosynthesis in plants. In this review, we systematically summarize the flavonoid biosynthetic pathway. We further assemble an exhaustive map of flavonoid biosynthesis in plants comprising eight branches (stilbene, aurone, flavone, isoflavone, flavonol, phlobaphene, proanthocyanidin, and anthocyanin biosynthesis) and four important intermediate metabolites (chalcone, flavanone, dihydroflavonol, and leucoanthocyanidin). This review affords a comprehensive overview of the current knowledge regarding flavonoid biosynthesis, and provides the theoretical basis for further elucidating the pathways involved in the biosynthesis of flavonoids, which will aid in better understanding their functions and potential uses.

Molecular biology of capsaicinoid biosynthesis in chili pepper (Capsicum spp.)

2010 ◽  
Vol 30 (5) ◽  
pp. 695-706 ◽  
Author(s):  
Cesar Aza-González ◽  
Hector G. Núñez-Palenius ◽  
Neftalí Ochoa-Alejo
Keyword(s):  
Molecular Biology ◽  
Chili Pepper ◽  
Capsicum Spp ◽  
Capsaicinoid Biosynthesis

scholarly journals Chili Pepper Carotenoids: Nutraceutical Properties and Mechanisms of Action

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5573
Author(s):  
Maria Guadalupe Villa-Rivera ◽  
Neftalí Ochoa-Alejo
Keyword(s):  
Cardiovascular Disorder ◽  
Chili Pepper ◽  
Carotenoid Content ◽  
Biosynthesis Pathway ◽  
Bioactive Components ◽  
Horticultural Crop ◽  
Treatment And Prevention ◽  
Capsicum Spp ◽  
General Aspects ◽  
Nutraceutical Properties

Chili pepper is a prominent cultivated horticultural crop that is traditionally used for food seasoning and is applied for the treatment and prevention of multiple diseases. Its beneficial health properties are due to its abundance and variety of bioactive components, such as carotenoids, capsaicinoids, and vitamins. In particular, carotenoids have important nutraceutical properties, and several studies have focused on their potential in the prevention and treatment of human diseases. In this article, we reviewed the state of knowledge of general aspects of chili pepper carotenoids (biosynthesis pathway, types and content in Capsicum spp., and the effects of processing on carotenoid content) and recent findings on the effects of carotenoid nutraceuticals, such as antioxidant, cancer preventive, anti-inflammatory, cardiovascular disorder preventive, and anti-obesity effects.

scholarly journals The Paleobiolinguistics of Domesticated Chili Pepper (Capsicum spp.)

2013 ◽  
Vol 4 ◽  
pp. 1-11 ◽  
Author(s):  
Cecil H. Brown ◽  
Charles R. Clement ◽  
Patience Epps ◽  
Eike Luedeling ◽  
Søren Wichmann
Keyword(s):  
Native American ◽  
Historical Linguistics ◽  
Comparative Method ◽  
Biological Species ◽  
Chili Pepper ◽  
Way Of Life ◽  
Broad Distribution ◽  
Geographic Space ◽  
Capsicum Spp ◽  
Chili Peppers

Paleobiolinguistics employs the comparative method of historical linguistics to reconstruct the biodiversity known to human groups of the remote, unrecorded past. Comparison of words for biological species from languages of the same language family facilitates reconstruction of the biological vocabulary of the family’s ancient proto-language. This study uses paleobiolinguistics to establish where and when chili peppers (Capsicum spp.) developed significance for different prehistoric Native American groups. This entails mapping in both time and geographic space proto-languages for which words for chili pepper reconstruct. Maps show the broad distribution of Capsicum through Mesoamerica and South America mirroring its likely independent domestication in these regions. Proto-language dates indicate that human interest in chili pepper had developed in most of Latin America at least a millennium before a village-farming way of life became widespread.

Community dynamics in highland watersheds

2017 ◽  
Author(s):  
Dean Jacobsen ◽  
Olivier Dangles
Keyword(s):  
High Altitude ◽  
Community Dynamics ◽  
Community Organization ◽  
Current Knowledge ◽  
Central Process ◽  
Spatial Variables ◽  
Research Areas ◽  
Local Extinctions ◽  
Metacommunity Dynamics ◽  
Natural Communities

Chapter 6 presents the interaction between space and time in determining the organization of natural communities in high altitude heterogeneous waterscapes. After explaining why high altitude waters represent suitable models for examining metacommunity organization, the chapter focuses on dispersal—a central process to allow colonization and establishment of populations in remote localities and to counter local extinctions. Community organization patterns are then described for a variety of organisms living in high altitude waters, from microbes to invertebrates to fish and birds. These patterns reveal that both environmental and spatial variables are generally involved in species assembling. Examples of studies on directional spatial processes (e.g. through wind and water flow), waterscape genetics, and temporal variability (synchrony/asynchrony) are highlighted as promising research areas to increase the current knowledge on high altitude metacommunity dynamics.

scholarly journals Transfer of Plasmid DNA to Clinical Coagulase-Negative Staphylococcal Pathogens by Using a Unique Bacteriophage

2015 ◽  
Vol 81 (7) ◽  
pp. 2481-2488 ◽  
Author(s):  
Volker Winstel ◽  
Petra Kühner ◽  
Bernhard Krismer ◽  
Andreas Peschel ◽  
Holger Rohde
Keyword(s):  
Plasmid Dna ◽  
Genetic Manipulation ◽  
Current Knowledge ◽  
Virulence Determinants ◽  
Coagulase Negative Staphylococci ◽  
Reliable Technique ◽  
Content Type ◽  
Research Activities ◽  
Wide Range ◽  
Genetic Barriers

ABSTRACTGenetic manipulation of emerging bacterial pathogens, such as coagulase-negative staphylococci (CoNS), is a major hurdle in clinical and basic microbiological research. Strong genetic barriers, such as restriction modification systems or clustered regularly interspaced short palindromic repeats (CRISPR), usually interfere with available techniques for DNA transformation and therefore complicate manipulation of CoNS or render it impossible. Thus, current knowledge of pathogenicity and virulence determinants of CoNS is very limited. Here, a rapid, efficient, and highly reliable technique is presented to transfer plasmid DNA essential for genetic engineering to important CoNS pathogens from a uniqueStaphylococcus aureusstrain via a specificS. aureusbacteriophage, Φ187. Even strains refractory to electroporation can be transduced by this technique once donor and recipient strains share similar Φ187 receptor properties. As a proof of principle, this technique was used to delete the alternative transcription factor sigma B (SigB) via allelic replacement in nasal and clinicalStaphylococcus epidermidisisolates at high efficiencies. The described approach will allow the genetic manipulation of a wide range of CoNS pathogens and might inspire research activities to manipulate other important pathogens in a similar fashion.

scholarly journals The MIR-Domain of PbbHLH2 Is Involved in Regulation of the Anthocyanin Biosynthetic Pathway in ”Red Zaosu” (PyrusBretschneideri Rehd.) Pear Fruit

2021 ◽  
Vol 22 (6) ◽  
pp. 3026
Author(s):  
Xieyu Li ◽  
Fangxin Xiang ◽  
Wei Han ◽  
Bingqing Qie ◽  
Rui Zhai ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Anthocyanin Biosynthesis ◽  
Bimolecular Fluorescence Complementation ◽  
Anthocyanin Content ◽  
Pear Fruit ◽  
Fruit Peel ◽  
Interaction Domain ◽  
Dihydroflavonol Reductase ◽  
Anthocyanin Biosynthetic Pathway ◽  
R2r3 Myb

The N-terminal of Myc-like basic helix-loop-helix transcription factors (bHLH TFs) contains an interaction domain, namely the MYB-interacting region (MIR), which interacts with the R2R3-MYB proteins to regulate genes involved in the anthocyanin biosynthetic pathway. However, the functions of MIR-domain bHLHs in this pathway are not fully understood. In this study, PbbHLH2 containing the MIR-domain was identified and its function investigated. The overexpression of PbbHLH2 in ”Zaosu” pear peel increased the anthocyanin content and the expression levels of late biosynthetic genes. Bimolecular fluorescence complementation showed that PbbHLH2 interacted with R2R3-MYB TFs PbMYB9, 10, and 10b in onion epidermal cells and confirmed that MIR-domain plays important roles in the interaction between the MIR-domain bHLH and R2R3-MYB TFs. Moreover, PbbHLH2 bound and activated the dihydroflavonol reductase promoter in yeast one-hybrid (Y1H) and dual-luciferase assays. Taken together these results suggested that the MIR domain of PbbHLH2 regulated anthocyanin biosynthesis in pear fruit peel.

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