scholarly journals COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758

2018 ◽  
Vol 3 (5) ◽  
pp. 54-59
Author(s):  
N. P. Peretolchina ◽  
A. Y. Borisenko ◽  
Yu. P. Dzhioev ◽  
V. I. Zlobin
Keyword(s):  
Public Health ◽  
Yersinia Pseudotuberculosis ◽  
Medical Science ◽  
Protein Structures ◽  
Far East ◽  
Database Search ◽  
Online Tools ◽  
Complete Genomes ◽  
Nucleotide Database ◽  
Cas Genes

Background. Pseudotuberculosis is still relevant problem in medical science and public health of Russia and other countries. Typing of Y. рseudotuberculosis strains by their CRISPR systems is a perspective tool for monitoring of Yersinia populations as was shown in Y. pestis.Aims. Here we describe and compare CRISPR-Cas systems of Yersinia pseudotuberculosis strains IP32953 and IP31758 causing classic pseudotuberculosis and Far-East scarlet-like fever (FESLF) respectively.Materials and methods. Complete genomes of Y. pseudotuberculosis IP329353 and IP31758 (NC_006155 and NC_009708 respectively) were obtained from NCBI Nucleotide Database. Search; identification; and analysis of CRISPR systems were carried out by online-tools CRISPROne; CRISPRDetect; and CRISPRTarget.Results and discussion. Analyzed strains have CRISPR-Cas systems that include one set of cas-genes and arrays situated at the long distances from each other. We defined three CRISPR arrays in Y. pseudotuberculosis IP32953 by the combination of program methods. CRISPR-Cas system of this strain consist of array YP1 located near cas-genes; arrays YP2 and YP3. CRISPR-Cas system of Y. pseudotuberculosis IP31758 includes two arrays – YP1 and YP3. CRISPR systems do not share similar spacers. CRISPR systems of the analyzed strains differ in CRISPR loci and cas-protein structures that can be used as specific marks of analyzed strains.Conclusions. We suggest that acquisition of certain spacers may play a role in evolution and divergence of Y. pseudotuberculosis strains.

scholarly journals In silico comparative analysis of crispr-cas system structures of Yersinia pseudotuberculosis causing different clinical manifestations of pseudotuberculosis

2019 ◽  
Vol 11 (2) ◽  
pp. 80-87
Author(s):  
N. P. Peretolchina ◽  
Yu. P. Dzhioev ◽  
A. Yu. Borisenko ◽  
L. A. Stepanenko ◽  
E. A. Voskresenskaya ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Yersinia Pseudotuberculosis ◽  
Protein Structures ◽  
Clinical Manifestations ◽  
Systemic Infection ◽  
Complete Genomes ◽  
Nucleotide Database ◽  
Cas Genes ◽  
Species Variability ◽  
Cas Proteins

The aim of this research was to analyze and compare CRIPSR loci and cas-proteins of Yersinia pseudotuberculosis strains isolated in different territories from patients with various clinical manifestations of pseudotuberculosis.Materials and Methods. Complete genomes of Y. pseudotuberculosis IP329353 (NC_006155) and IP31758 (NC_009708) were obtained from NCBI Nucleotide Database. Strains were isolated from patients with gastroenteritis and systemic infection respectively. Search, identification, and analysis of CRISPR systems were carried out by onlinetools CRISPROne, CRISPRDetect, and CRISPRTarget.Results. Analyzed strains have CRISPR-Cas systems that include one set of cas-genes and arrays situated at the long distances from each other. We defined three CRISPR arrays in Y. pseudotuberculosis IP32953: array YP1 located near cas-genes, arrays YP2 and YP3. CRISPR-Cas system of Y. pseudotuberculosis IP31758 includes two arrays – YP1 and YP3. CRISPR systems do not share similar spacers.Conclusion. CRISPR systems of the analyzed strains differ in CRISPR loci and cas-protein structures that can be used as specific molecular marks of analyzed strains during the study of intra-species variability and evolution of Y. pseudotuberculosis.

scholarly journals Chikungunya virus outbreak-a threat to global public health including Bangladesh

2018 ◽  
Vol 17 (2) ◽  
pp. 183-184 ◽  
Author(s):  
Abdullah Al Mahdy ◽  
Mostofa Jamal ◽  
Hiroshi Kinoshita ◽  
Tareq Hossan
Keyword(s):  
Public Health ◽  
Chikungunya Virus ◽  
Medical Science ◽  
Global Public Health

Abstract not availableBangladesh Journal of Medical Science Vol.17(2) 2018 p.183-184

scholarly journals Thematic atlas of Italian oncological research: the analysis of public IRCCS

2021 ◽  
pp. 109-114
Author(s):  
Corrado Cuccurullo ◽  
Luca D’Aniello ◽  
Maria Spano
Keyword(s):  
Public Health ◽  
Health System ◽  
Quantitative Research ◽  
Public Health System ◽  
Medical Science ◽  
Large Body ◽  
Past Research ◽  
Research Area ◽  
Big Picture ◽  
The Many

This paper has been developed in the frame of the research project “V:ALERE 2019” focused on Italian public-owned Academic Medical Centers. The main aim of the project is to provide evidence, advice, and remarks to help the agents of the public health system to address the many challenges that they face. In recent years, there is an increasing recognition of the potential value of research evidence as one of the many factors considered by policymakers and practitioners. Even more, in the case of medical science, the analysis of research and its impact is indispensable, in light of its implications for public health. The starting point for mapping a research area is to review the related scientific literature because by synthesizing past research findings, it is possible to effectively use the existing knowledge base and advance lines of future researches. In this sense, bibliometrics becomes useful, by providing a structured analysis to a large body of information, to infer trends over time, themes researched, and to show the “big picture” of extant research. In particular, in this work, we focus our attention on the scientific production of the last 20 years of the Scientific Institutes for Research, Hospitalization, and Healthcare (IRCCS “Istituto di Ricovero e Cura a Carattere Scientifico”) specialized in the oncology research. IRCCS are biomedical institutions of relevant national interest that drive clinical assistance in strong relation to research activities. They are committed to being a benchmark for the whole public health system for both the quality of patient care and the innovation skills in the field of the organization. All the analyses were carried out by using the Bibliometrix, an open-source tool for quantitative research in scientometrics and bibliometrics that includes all the main bibliometric methods of analysis.

Mastitis Caused by Yersinia pseudotuberculosis in Israeli Dairy Cattle and Public Health Implications

2007 ◽  
Vol 54 (9-10) ◽  
pp. 353-357 ◽  
Author(s):  
A. Shwimmer ◽  
M. Freed ◽  
S. Blum ◽  
N. Khatib ◽  
L. Weissblit ◽  
...  
Keyword(s):  
Public Health ◽  
Dairy Cattle ◽  
Yersinia Pseudotuberculosis ◽  
Health Implications

scholarly journals Leptin signaling regulates physiological damage and host-pathogen cooperation

2020 ◽  
Author(s):  
Karina K. Sanchez ◽  
Katia Troha ◽  
Sarah Stengel ◽  
Janelle S. Ayres
Keyword(s):  
Defense Mechanisms ◽  
Yersinia Pseudotuberculosis ◽  
Far East ◽  
Organ Damage ◽  
Resistance Mechanisms ◽  
Defense Strategies ◽  
Leptin Signaling ◽  
Host Pathogen ◽  
Cooperative Defense ◽  
Additional Level

ABSTRACTTo combat infections, hosts employ a combination of antagonistic and cooperative defense strategies. The former refers to pathogen killing mediated by resistance mechanisms, while the latter refers to physiological defense mechanisms that promote host health during infection independent of pathogen killing, leading to an apparent cooperation between the host and the pathogen. Previous work has shown that leptin, a pleiotropic hormone that plays a central role in regulating appetite and energy metabolism, is indispensable for resistance mechanisms, while a role for leptin signaling in cooperative host-pathogen interactions remains unknown. Using a mouse model of Yersinia pseudotuberculosis (Yptb) infection, the causative agent of Far East scarlet-like fever, we unexpectedly found that genetic inhibition of leptin signaling conferred protection from Yptb infection due to increased host-pathogen cooperation rather than greater resistance defenses. The protection against Yptb infection was not due to differences in food consumption, lipolysis or fat mass. Furthermore, we found that the survival advantage was associated with increased liver damage and dysfunction. Our work reveals an additional level of complexity for the role of leptin in infection defense and suggests that in some contexts, in addition to tolerating the pathogen, tolerating organ damage and dysfunction is more beneficial for survival than preventing the damage.

The importance of mass communication in promoting positive health

2010 ◽  
pp. 108-111
Author(s):  
Thomas Lom
Keyword(s):  
Public Health ◽  
Health Care ◽  
Health Care Professionals ◽  
Public Health Policy ◽  
Mass Communication ◽  
Medical Science ◽  
Diagnostic Tools ◽  
Positive Health ◽  
For Profit ◽  
New Treatments

Medical science is enabling an explosion of discovery in diagnostic tools and in the development of new treatments and products. But how do we take advantage if we are not aware? That is where the power of mass communication comes into play. In a world with increasingly motivated and empowered patients, these go well beyond just the for-profit companies such as the pharmaceutical industry and include payers, governments, health care professionals, and institutions. The motivation for the dissemination of health information is a convergence of public health interest and public health policy with private sector commercial interests....

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