scholarly journals Adaptive Measured Model for Cell Adhesive Force Using Dielectrophoresis Force

Author(s):  
C.C. Lien ◽  
C. Ay ◽  
J.Y. Cheng ◽  
C.W. Young
Keyword(s):  
Sensors ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 7128
Author(s):  
Ying Tu ◽  
Xuefeng Wang

Cell adhesive force, exerting on the local matrix or neighboring cells, plays a critical role in regulating many cell functions and physiological processes. In the past four decades, significant efforts have been dedicated to cell adhesive force detection, visualization and quantification. A recent important methodological advancement in cell adhesive force visualization is to adopt force-to-fluorescence conversion instead of force-to-substrate strain conversion, thus greatly improving the sensitivity and resolution of force imaging. This review summarizes the recent development of force imaging techniques (collectively termed as cell adhesive force microscopy or CAFM here), with a particular focus on the improvement of CAFM’s spatial resolution and the biomaterial choices for constructing the tension sensors used in force visualization. This review also highlights the importance of DNA-based tension sensors in cell adhesive force imaging and the recent breakthrough in the development of super-resolution CAFM.


2020 ◽  
Author(s):  
Debayan Dasgupta ◽  
Dharma Pally ◽  
Deepak K. Saini ◽  
Ramray Bhat ◽  
Ambarish Ghosh

The dissemination of cancer is brought about by continuous interaction of malignant cells with their surrounding tissue microenvironment. Understanding and quantifying the remodeling of local extracellular matrix (ECM) by invading cells can therefore provide fundamental insights into the dynamics of cancer dissemination. In this paper, we use an active and untethered nanomechanical tool, realized as magnetically driven nanorobots, to locally probe a 3D tissue culture microenvironment consisting of cancerous and non-cancerous epithelia, embedded within reconstituted basement membrane (rBM) matrix. Our assay is designed to mimic the in vivo histopathological milieu of a malignant breast tumor. We find that nanorobots preferentially adhere to the ECM near cancer cells: this is due to the distinct charge conditions of the cancer-remodeled ECM. Surprisingly, quantitative measurements estimate that the adhesive force increases with the metastatic ability of cancer cell lines, while the spatial extent of the remodeled ECM was measured to be approximately 40 μm for all cancer cell lines studied here. We hypothesized and experimentally confirmed that specific sialic acid linkages specific to cancer-secreted ECM may be a major contributing factor in determining this adhesive behavior. The findings reported here can lead to promising applications in cancer diagnosis, quantification of cancer aggression, in vivo drug delivery applications, and establishes the tremendous potential of magnetic nanorobots for fundamental studies of cancer biomechanics.


1991 ◽  
Vol 266 (5) ◽  
pp. 3045-3051
Author(s):  
F Kimizuka ◽  
Y Ohdate ◽  
Y Kawase ◽  
T Shimojo ◽  
Y Taguchi ◽  
...  

1989 ◽  
Vol 264 (26) ◽  
pp. 15165-15168
Author(s):  
T Maeda ◽  
R Oyama ◽  
K Ichihara-Tanaka ◽  
F Kimizuka ◽  
I Kato ◽  
...  

1987 ◽  
Vol 262 (7) ◽  
pp. 2957-2967 ◽  
Author(s):  
J.A. McDonald ◽  
B.J. Quade ◽  
T.J. Broekelmann ◽  
R. LaChance ◽  
K. Forsman ◽  
...  

Metals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1021
Author(s):  
Mauro Andres Cerra Florez ◽  
Gemma Fargas Ribas ◽  
Jorge Luiz Cardoso ◽  
Antonio Manuel Mateo García ◽  
Joan Josep Roa Rovira ◽  
...  

Aging heat treatments in maraging steels are fundamental to achieve the excellent mechanical properties required in several industries, i.e., nuclear, automotive, etc. In this research, samples of maraging 300 alloy were aged using a novel procedure that combines different steps with two atmospheres (nitrogen and water vapor) for several hours. The oxidized surface layer was chemical, microstructural and micromechanically characterized. Due to the thermodynamic and kinetic conditions, these gases reacted and change the surface chemistry of this steel producing a thin iron-based oxide layer of a homogeneous thickness of around 500 nm. Within the aforementioned information, porosity and other microstructural defects showed a non-homogeneous oxide, mainly constituted by magnetite, nickel ferrite, cobalt ferrite, and a small amount of hematite in the more external parts of the oxide layer. In this sense, from a chemical point of view, the heat treatment under specific atmosphere allows to induce a thin magnetic layer in a mixture of iron, nickel, and cobalt spinel ferrites. On the other hand, the oxide layer presents an adhesive force 99 mN value that shows the capability for being used for tribological applications under sliding contact tests.


2020 ◽  
Vol 982 ◽  
pp. 195-200
Author(s):  
Abdullah Al Mamun ◽  
Okan Sirin

Nanotechnology has contributed significantly to different subfields of the construction industry, including asphalt pavement engineering. The improved properties and new functionalities of the nanomaterials have provided different desired properties of asphalt. In this study, the effectiveness of multi-walled carbon nanotubes (MWCNT) in resisting the oxidation of polymer-modified asphalt was measured. A total of three different percentages (0.5%, 1%, and 1.5%) of MWCNT were used to modify the Styrene-Butadiene (SB) and styrene–butadiene–styrene (SBS) modified asphalt (4% and 5%). The laboratory oxidized asphalt samples were evaluated by an atomic force microscopy machine. The oxidation of the polymer-MWCNT modified asphalt is measured by simulating the existing functional group of the asphalt and as a function of the adhesive force. It is observed that the use of MWCNT in SB and SBS can increase the resistance to oxidation.


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