scholarly journals Study About Pathogenic Bacteria Associated With Bovine Mastitis

2004 ◽  
Vol 28 (1) ◽  
pp. 227-234
Author(s):  
Safana A. S. AL-Taan ◽  
Asmaa H. AL-Jobori ◽  
Anton S. AL-Bana.
Keyword(s):  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Bovine Mastitis ◽  
Raw Milk ◽  
Viable Count ◽  
Coliform Bacteria ◽  
Milk Samples ◽  
Different Types ◽  
High Level

Out of 100 raw milk samples collected from one hundred cows infected with mastitis, thirty three isolates of different types of pathogenic bacteria were isolated using different types of selective media.  On the basis of traditional bacteriological tests, these isolates were identified as the following: 13 isolates of Staphylococcus aureus, 9 isolates of B-haemolytic Streptococci, 6 isolates of Coliform bacteria, 4 isolates of Corynebacterium pyogenes, and only one isolate Bacillus cereus.  In addition to these isolates, a number of non-pathogenic bacteria were recovered during the isolation and they were considered as contaminants of milk such as S. epidermidis, C. bovis and B. subtilus.  The total number of living bacteria present in milk samples was measured and it was ranged from (1400 to 1800) bact./ml.  The isolates of pathogenic bacteria were tested for their sensitivity to different types of antibiotics and the results were: 100% of these isolates were sensitive to Gentamycin and Tetracycline, 11 (84.6%) isolates of S. aureus, 6(66.2%) isolates of B-hacmolytic Streptococci , 4(66.2%) isolates of Coliform bacteria and 3(75%)  es of C. pyogenes were sensitive to Ampicillin, Penicillin, Erythromycin and Cepholexin. While Bacillus cereus isolate was resistant to all these antibiotics.  As a result of this work, generally the bacteriological quality of this milk is acceptable due to the low number of viable count of bacteria in  milk and the isolates showed high level of sensitivity to the antibiotics used in this study.

scholarly journals Study About Pathogenic Bacteria Associated With Bovine

2004 ◽  
Vol 28 (1) ◽  
pp. 227-234
Author(s):  
Safana A. S. AL-Taan ◽  
Asmaa H. AL-Jobori ◽  
Anton S. AL-Bana.
Keyword(s):  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Viable Count ◽  
Coliform Bacteria ◽  
Milk Samples ◽  
Different Types ◽  
High Level ◽  
Living Bacteria

Out of 100 raw milk samples collected from one hundred cows infected with mastitis, thirty three isolates of different types of pathogenic bacteria were isolated using different types of selective media.  On the basis of traditional bacteriological tests, these isolates were identified as the following: 13 isolates of Staphylococcus aureus, 9 isolates of B-haemolytic Streptococci, 6 isolates of Coliform bacteria, 4 isolates of Corynebacterium pyogenes, and only one isolate Bacillus cereus.  In addition to these isolates, a number of non-pathogenic bacteria were recovered during the isolation and they were considered as contaminants of milk such as S. epidermidis, C. bovis and B. subtilus.  The total number of living bacteria present in milk samples was measured and it was ranged from (1400 to 1800) bact./ml.  The isolates of pathogenic bacteria were tested for their sensitivity to different types of antibiotics and the results were: 100% of these isolates were sensitive to Gentamycin and Tetracycline, 11 (84.6%) isolates of S. aureus, 6(66.2%) isolates of B-hacmolytic Streptococci , 4(66.2%) isolates of Coliform bacteria and 3(75%)  es of C. pyogenes were sensitive to Ampicillin, Penicillin, Erythromycin and Cepholexin. While Bacillus cereus isolate was resistant to all these antibiotics.  As a result of this work, generally the bacteriological quality of this milk is acceptable due to the low number of viable count of bacteria in   milk and the isolates showed high level of sensitivity to the antibiotics used in this study.

scholarly journals Physical and microbial qualities of raw milk collected from Bangladesh Agricultural University dairy farm and the surrounding villages

1970 ◽  
Vol 6 (2) ◽  
pp. 217-221 ◽  
Author(s):  
MTG Khan ◽  
MA Zinnah ◽  
MP Siddique ◽  
MHA Rashid ◽  
MA Islam ◽  
...  
Keyword(s):  
Microbiological Quality ◽  
Dairy Farm ◽  
Raw Milk ◽  
Viable Count ◽  
Physical Parameters ◽  
Total Viable Count ◽  
Coliform Count ◽  
Milk Samples

The present study was undertaken with the aim of investigating the physical parameters (e.g. organoleptic and specific gravity of raw milk) and also to study the microbiological quality of raw milk (total viable count, Coliform count and Staphylococcal count) from different villages and Bangladesh Agricultural University (BAU) Dairy Farm of Mymensingh District of Bangladesh, during the period from July to November 2007. A total number of 100 raw milk samples were collected at morning and evening from BAU dairy farm and surrounding four villages of BAU campus. The organoleptic and bacteriological qualities of each sample were analyzed. The organoleptic examination included taste panel score to assess consumer's acceptance and the bacteriological analysis comprised enumeration of total viable count (TVC), total colifrom count (TCC) and total staphylococcal count (TSC) for the determination of sanitary quality. The organoleptic quality of the milk samples is more or less same except the Churkhai milk samples which had flat taste (in 16% milk sample). The average values of TVC/ml were log 5.920, 5.934, 6.007, 6.075 and 6.127 for BAU Dairy Farm, Boira, Shutiakhali, Churkahai and Paglabazar respectively; coliform count were log 2.501, 2.522, 2.550, 2.620 and 2.619 respectively; staphylococcal count were log 2.832, 2.812, 2.866, 2.931 and 2.988 respectively. So, it may be concluded that the raw milk samples of BAU Dairy Farm were superior to others collected from the selected villages which may be due to maintaining better hygienic condition. Key words: Raw milk, physical and microbial quality   doi: 10.3329/bjvm.v6i2.2339 Bangl. J. Vet. Med. (2008). 6 (2): 217-221

scholarly journals Public Health Safety in Relation to Microbiological Quality of Freshly Drawn Cow’s Milk in Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 231-236
Author(s):  
R Khaton ◽  
MA Hasnat ◽  
S Rahman ◽  
MM Rahman
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Viable Count ◽  
Microbial Quality ◽  
Total Viable Count ◽  
Coliform Count ◽  
Milk Samples

The aim of the present study was to determine the microbial quality of freshly drawn cow’s milk belonging to the arsenic affected and non-affected areas. In the present work 25 milk sample were collected from five different arsenic affected and non-affected areas. The analysis comprised enumeration of total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC) for the determination of sanitary quality. The highest total viable count, total coliform count and total staphylococcus count were log 5.894 ± 0.221, log 2.832± 0.129 and log 2.898 ± 0.162 respectively. Staphylococcus spp, Escherichia coli, Pseudomonas spp and Bacillus spp were isolated from the milk samples. Among them Escherichia coli were isolated from the maximum milk samples. It is found that the level of microbial quality in terms of TVC, TCC and TSC were high in arsenic affected areas than arsenic non-affected area. It also found that TVC and TCC were positively correlated with each other. This survey indicates that most of the raw milk samples were not satisfactory in course of public health standard as some pathogenic bacteria were detected from these samples and it is necessary to improve the hygienic practices required for the handling and processing of milk.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21297 Bangl. J. Vet. Med. (2014). 12 (2): 231-236 

scholarly journals Detection of pathogenic bacteria from raw, pasteurized and UHT milk available in the local market of Gazipur District

2018 ◽  
Vol 7 (2) ◽  
pp. 13-19
Author(s):  
Nazia Afrin ◽  
SM Rokon Ud Doula ◽  
Rasheda Yasmin Shilpi
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Local Market ◽  
Pseudomonas Sp ◽  
Gram Negative ◽  
Uht Milk ◽  
Milk Samples ◽  
Acceptable Range ◽  
Public Health Standard

The present study was undertaken with the aim of investigating the bacteriological quality of locally available raw, pasteurized and UHT milk in Gazipur District. A total 5 raw, pasteurized and UHT milk samples were collected during November to December 2016. All the pasteurized and UHT milk samples showed the total aerobic heterotrophic bacterial (TAHB) level above the standard acceptable range (i.e. > 104 cfu/ml for pasteurized milk and > 0 for UHT milk). Both Gram positive and Gram negative pathogenic bacteria viz. Staphylococcus aureus, S. epidermidis, Bacillus sp., Pseudomonas sp. and Enterobacter sp. were isolated from studied samples. API 20 E was used for confirmation of Pseudomonas sp. This study indicates that most of the pasteurized and UHT milk samples were not satisfactory in terms of public health standard. Therefore, care must be taken to avoid possible health risk in every step involving collection, transportation, storage and post pasteurization handing of raw milk. Jahangirnagar University J. Biol. Sci. 7(2): 13-19, 2018 (December)

scholarly journals Quality of cows’ milk from organic and conventional farming 

2014 ◽  
Vol 32 (No. 4) ◽  
pp. 398-405 ◽  
Author(s):  
L. Kouřimská ◽  
V. Legarová ◽  
Z. Panovská ◽  
J. Pánek
Keyword(s):  
Freezing Point ◽  
Farming Systems ◽  
Preference Test ◽  
Raw Milk ◽  
Coliform Bacteria ◽  
Conventional Farming ◽  
Milk Samples ◽  
Whole Milk ◽  
Organic And Conventional Farming

The results of chemical and microbial analyses of raw milk samples coming from organic and conventional farming systems were statistically compared. The samples were analysed during a twelve-month period (June–May). A total of 2206 samples were collected of which 528 were organic. After raw milk was processed, sensory hedonic quality of 171 pairs of organic and conventional non-standardised pasteurised whole milk samples were evaluated using the pair comparison preference test. Four parameters in raw milk, free fatty acids, urea content, somatic cell count and coliform bacteria count, showed no significant differences between the two types of production. Significantly higher contents of protein, casein, lactose, and non-fat solids were detected in conventional milk, which also had a significantly lower freezing point. On the contrary, significantly higher contents of total mesophilic bacteria count and a higher percentage of samples with positive coliform bacteria count were found in organic milk samples. Sensory analysis of pasteurised milk showed no significant hedonic difference between organic and conventional samples.

scholarly journals Microbiological Quality of Market Fruits and Their Handling Implication in Relation to Public Health

2013 ◽  
Vol 1 (2) ◽  
pp. 76-80
Author(s):  
Mohammad Kamrul Alam ◽  
Mahbubul Pratik Siddique ◽  
Muhammad Tofazzal Hossain ◽  
Md Atawar Rahman ◽  
Md Shahidur Rahman Khan
Keyword(s):  
Public Health ◽  
Pathogenic Bacteria ◽  
Research Study ◽  
Microbiological Quality ◽  
Viable Count ◽  
Coliform Bacteria ◽  
Public Health Implication ◽  
Urban Market ◽  
Fruit Samples

The research study was conducted on the market fruits from selected local markets of Mymensingh, Bangladesh. The objectives of the study were to assess the microbiological quality of market fruits, hand washing and used water of hotels and chatpoti shops and to predict the comparative public health implication associated with the socioeconomic status of the inhabitants consuming the fruits and water. In fruit samples, the total viable count ranged from log 2.90 to log 6.4. No coliform bacteria were detected in fruit samples. Statistical analysis showed significant variations in microbial load in different fruits, hand washings and used water. Present study further demonstrated that there was lack of proper hygienic and sanitation measures in most of the markets, especially in town markets. Because of observed low sanitary quality, the urban market fruits should be handled and used with special attention to reduce the risk of contamination with potential pathogenic bacteria. DOI: http://dx.doi.org/10.3329/mh.v1i2.14095 Microbes and Health, 2012 1(2): 76-80

scholarly journals The Physicochemical Quality of Traditional Burduf Cheese

2013 ◽  
Vol 70 (2) ◽  
pp. 137
Author(s):  
Carmen Pop ◽  
Cristina Anamaria Semeniuc ◽  
Sorin Apostu ◽  
Ancuţa Mihaela Rotar
Keyword(s):  
Quality Control ◽  
Raw Milk ◽  
Cow Milk ◽  
Chemical Parameters ◽  
Physicochemical Quality ◽  
Milk Samples ◽  
Sheep Milk ◽  
Physico Chemical ◽  
Different Types

The aim of this study is the assessmentof the quality control of raw milk and traditional burduf cheese obtained fromcow milk mixed with 10% sheep milk. Appreciation of the integrity and freshness assessmentof milk (cow and sheep) was tested by physico-chemical analysis.On theshelf-live were determined the physico-chemical parameters in cheese samples. Theantibiotics residues were tested of the milk samples with portable analyser,model Rosa Charm Reader. Theresults of physico-chemical determinations for the milk and cheese samples werewithin the maximum permissible by data legislation. Regardingthe content of antibiotics, the results were negative both for cow milk and forsheep milk. The sensorycharacteristics of burduf cheese are influenced by the different types of milk.

The Effectiveness of Amoxicillin and Cefalexin in Two Species from Staphylococcus and Bacillus Isolated from Bovine Mastitis in Sudan

2019 ◽  
Author(s):  
Reem Rabie Mohammed Salih
Keyword(s):  
Bacillus Cereus ◽  
Bovine Mastitis ◽  
Bacillus Coagulans ◽  
Staph Aureus ◽  
Milk Samples ◽  
Sensitivity Tests ◽  
Bacillus Spp ◽  
Staphylococcus Spp

In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial agents. The percentage of isolates was as follows: Staphylococcus spp 52%, Bacillus spp 26%, Enteroccocispp 3%; microccus 3%, Nesseria 2%, Branhamella 3%, Clostridium 1%, Bordetella 2%, Enterobacteria 3%, Aeromonas 4%, Fusobacterium 1%. In sensitivity tests used two antibiotics Amoxicillin and Cephalexin against two highest isolates in this study (Staph spp and Bacillus spp and found: Staph. aureus and Staph. hyicus were resistant to Amoxicllin and cephlaxin relatively, Bacillus coagulans and Bacillus cereus were susceptible to Amoxicllin and cephlaxin.

scholarly journals Quality of ultra-high temperature treated milk available in Gazipur and Mymensingh of Bangladesh

2016 ◽  
Vol 44 (3) ◽  
pp. 132-136
Author(s):  
MY Arafat ◽  
R Habib ◽  
MSR Siddiki ◽  
MF Imam
Keyword(s):  
High Temperature ◽  
Specific Gravity ◽  
Viable Count ◽  
Total Viable Count ◽  
Total Solids ◽  
Uht Milk ◽  
Coliform Count ◽  
Milk Samples ◽  
Ultra High Temperature

This experiment was conducted to evaluate the quality of existing ultra-high temperature (UHT) treated milk available in two selected town markets of Bangladesh. A total of 27 UHT milk samples from three different brands–Pran Dairy (A), Aarong Dairy (B) and Farm Fresh (C) were collected from local markets at Mymensingh and Gazipur districts during the period of 1stSeptember to 29th November, 2014. Parameters studied in this experiment were organoleptic (flavour, consistency, colour and appearance), physic-chemical (specific gravity, acidity, total solids, solids-not-fat, fat, protein, lactose, and ash) and microbiological (Total Viable Count and Coliform Count). All the samples were similar in respect of color, flavor, taste and texture, and no significant difference was observed. However, significant differences were revealed (p<0.05) in case of specific gravity, acidity, total solids, solids-not-fat, and protein content. On the other hand, insignificant differences were found (p>0.05) in case of fat, lactose, and ash content. Total Viable Count and Coliform count were found to be nil in all the milk samples. Milk samples of Farm Fresh UHT milk were superior to other brands of UHT Milk in terms of contents of total solids (119.23±0.57 g/kg),fat (34.97±0.35g/kg), lactose (43.23±0.51g/kg), and ash (7.00±0.26g/kg). Though there were some fluctuations in all the parameters studied, all the milk samples conformed to the standard values for UHT milk, and in general all of the UHT milk samples studied in this experiment was of good quality.Bang. J. Anim. Sci. 2015. 44 (3):132-136

scholarly journals Assessment of the microbiological quality of popular food items on sale in secondary school canteens of Mauritius

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Dayawatee Goburdhun ◽  
Mahima D. Beeharry ◽  
Keshnee Reega ◽  
Arvind Ruggoo ◽  
Hudaa Neetoo
Keyword(s):  
Secondary School ◽  
Microbiological Quality ◽  
Viable Count ◽  
Food Type ◽  
Salmonella Spp ◽  
E Coli ◽  
High Level ◽  
Microbiological Analyses ◽  
Composite Samples

This study was carried out to assess the microbiological status of three hot meals served in eight selected school canteens of Mauritius, with two schools randomly selected from each of the four school zones of the island. Three individual samples of farata, panini, or fried noodles were collected at each school during two independent visits. The three individual samples of each food type collected during each visit were then pooled before being subjected to microbiological analyses. A total of 48 composite samples were analyzed. The parameters tested were Total Viable Count (TVC), Escherichia coli, Salmonella spp., Clostridium perfringens, Staphylococcus aureus, and Listeria spp. The microbiological analyses revealed that paninis were deemed as generally acceptable with TVC falling in the range of 3.0-5.7 Log CFU/g and undetectable levels of S. aureus and E. coli. In contrast, fried noodles and faratas harboured a moderately high level of TVC (4.4-6.7 Log CFU/g) and objectionably high levels S. aureus (3.1 to 5.0 Log CFU/g) and E. coli (3.1-5.1 Log CFU/g) for seven out of the eight schools.

Sign in / Sign up

Export Citation Format

Share Document