Comparison of microbial isolates isolated from external ear canal of sheep and their susceptibility to antibiotics

One hundred sheep with bilateral otitis externa were studied one year and a half .Theexudates of both external ears obtained using sterile swabs, and microorganisms were isolatedaccording to standard microbiological techniques. There was bacterial and fungal growth inmany of the samples. The total numbers of isolates from right ear was 104 , and the mostcommon pathogens isolated in right ear were Staphylococcus aureus 30.76 % , thenStaphylococcus epidermidis 9.61% and Pseudomonas aeruginosa 9.61 % while E.coli ,Proteus mirabilis and Streptococcus pyogenes were 7.69% for each of them , Candidaalbicans 5.76% , Klebsiella pneumonia 3.84% ,Nocardia spp 3.84% , Pasteurella multucida3.84% ,Mannheimia haemolytica 3.84% , Aspergillus spp. 3.84 % and Streptomyces spp.1.23% . The total number of isolates from left ear was 96 ,the most pathogens wereStaphylococcus aureus 27.08% while Pseudomonas aeruginosa 12.5% and Streptococcuspyogenes were 12.5% , Klebsiella pneumonia 8.33 % and Proteus mirabilis 8.33 % ,Staphylococcus epidermidis , Nocardia spp 6.25 % , then Streptomyces spp , Pasteurellamultucida and Mannheimia haemolytica were 4.16% , the last E.coli , Aspergillus spp andCandida albicans were 2.08 % for each of them . The result were showed significantdifferences at level P ˂0.05 between bacteria and fungi isolates, and have no significantdifferences at level P˃ 0.05 between right and left ear . High susceptible rates of grampositive Staphylococcus aureus to (Amoxicillin + Clavulaunic acid) and Chloramphenicolwere 100 % , while high susceptible rates of gram negative Pseudomonas aeruginosa was90.90 % to Ciprofloxacin, (Amoxicillin+ Clavulaunic acid) and Chloramphenicol .

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Microbiological Study of Diabetic Foot Ulcer

Anwer Khan Modern Medical College Journal ◽

10.3329/akmmcj.v10i1.43659 ◽

2019 ◽

Vol 10 (1) ◽

pp. 50-55

Author(s):

Mohammuddunnobi ◽

Tasnuva Jahan ◽

Abdullah Al Amin

Keyword(s):

Diabetes Mellitus ◽

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Proteus Mirabilis ◽

Diabetic Foot ◽

Foot Ulcer ◽

Diabetic Foot Ulcer ◽

Klebsiella Pneumonia ◽

Coagulase Negative Staphylococci

Background: Diabetic foot is one of the most feared complications of diabetes and is the leading cause of hospitalization in diabetic patients. Limb-threatening diabetic infections are usually polymicrobial involving multiple aerobic and anaerobic organisms. Methodology: The present study was a cross sectional study, conducted in the department of surgery and microbiology at BIRDEM General Hospital, Dhaka, over a period of 9 months during January 2017- September' 2017. The study included a total of 77 adult patients of clinically diagnosed diabetic foot patients presenting to outpatient department and emergency ward. The standard case definition of diabetic foot is 'any pathology occurring in the foot of a patient suffering from diabetes mellitus or as a result of long term complication of diabetes mellitus'. Results: Majority 17(22.1%) patients had Klebsiella pneumonia, 14(18.2%) had Pseudomonas aeruginosa, 11(14.3%) had Staphylococcus aureus, 10(13.0%) had Escherichia coli, 6(7.8%) had Coagulase-negative staphylococci and 8(10.4%) had Providencia spp. In Escherichia coli 100% sensitivity to imipenem, 70% to amoxicillin-clavulanic acid, amikacin, piperacillin-tazobactam. In Coagulasenegative Staphylococci 83.3% sensitivity to tetracycline, 66.7% to ceftriaxone. In Proteus mirabilis 100% sensitivity to tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam. In Enterococcus spp.75.0% sensitivity to tetracycline. In Citrobacter spp. 100% sensitivity to imipenem. Conclusion: Common organism found in diabetic foot ulcer patients were Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Coagulase-negative staphylococci and Providencia spp. In tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam was 100% sensitive in Proteus mirabilis and only imipenem found in Escherichia coli and Citrobacter spp. Anwer Khan Modern Medical College Journal Vol. 10, No. 1: Jan 2019, P 50-55

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Chicken feather hydrolysate as alternative peptone source for microbial cultivation

F1000Research ◽

10.12688/f1000research.17134.1 ◽

2018 ◽

Vol 7 ◽

pp. 1918 ◽

Cited By ~ 1

Author(s):

Oghenerobor B. Akpor ◽

Damilola E. Odesola ◽

Remilekun E. Thomas ◽

Olarewaju M. Oluba

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Proteus Mirabilis ◽

Protein Hydrolysate ◽

Test Organism ◽

Maximum Growth ◽

Chicken Feather ◽

E Coli ◽

Bacteria And Fungi

Background: Commercially available conventional growth medium for the culture of microbes are expensive, hence the need for alternative cheaper sources. Poultry waste, in the form of feather and blood, are of value in biotechnology because of their high protein content. Hence the primary aim of this study was to produce a cheaper peptone alternative from chicken feather protein hydrolysate (CFPH) and blood meal (BM). Methods: We monitored the growth of selected bacteria and fungi in different concentrations of medium produced from varying combination of peptone, CFPH and BM in order to determine the combination that produced maximum growth. Five different media, namely 100% peptone (control), 100% BM, 40% peptone + 60% CFPH, 40% BM + 60% CFPH and 20% peptone + 20% BM + 60% CFPH were prepared and used for the study. The different media were inoculated with 1 ml of each test organism (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida carpophila, Candida tropicalis and Pichia kundriavzevii) and their growth monitored for 10 h. Results: Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus grew best in the 100% peptone, Klebsiella pneumoniae grew best in 100 BM. The fungi species were observed to grow best in 100% peptone. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of TCA, HCl, and HNO3 gave the best growth of E. coli. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of TCA) also gave the best growth of C. tropicalis and Klebsiella pneumoniae respectively. Conclusions: Overall, the 60% CFPH + 40% peptone combination showed the most potential as an alternative to peptone, especially for E. coli.

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A Study of Prevalence of Aerobic Bacteria and Fungi in Sputum Specimens of Patients with Post Tubercular Bronchiectasis

International Journal of Medical and Dental Sciences ◽

10.18311/ijmds/2017/18834 ◽

2017 ◽

Vol 6 (2) ◽

pp. 1468

Author(s):

Gattu Suresh Kumar ◽

Sabinkar Babu Lal ◽

M. Laxmikanth

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Antibiotic Sensitivity ◽

Aerobic Bacteria ◽

Klebsiella Pneumonia ◽

Culture Methods ◽

Resistance Patterns ◽

Bacteria And Fungi ◽

One Year

Background: Patients with Post TB bronchiectasis colonize many aerobic bacteria and fungi and lead to an increase in exacerbations and decrease in quality of life.Objective: To study the prevalence of aerobic bacteria and fungi in sputum specimens of patients with post tubercular bronchiectasis and to find out the local antibiotic sensitivity and resistance patterns.Materials and Methods: This prospective observational study was carried out over a period of one year comprising of 50 patients with post Tubercular Bronchiectasis who were above 18 yrs, completed ATT smear negative and HRCT showing bronchiectactic changes. Identification of organisms from sputum samples were done by classical aerobic microbial staining and culture methods. Descriptive and inferential statistical analysis was carried out in this study.Results: This study comprises of the 50 patients with a larger group of individuals under the age group of 51-60 (30%), along with bronchial colonization of aerobic bacteria being 78% and growth of fungi being 2%.Among the 39 patients,16 patients (32%) had grown Pseudomonas aeruginosa, 12 patients(24%) had grown Klebsiella pneumoniae, 8 patients(16%) had grown Streptococcus species and rest 3 patients(6%) had grown staphylococcus species. The Antibiotic resistance noted highest being Amikacin (56.4%), piperacillin-tazobactam showing the least (2.6%) and highest sensitivity with imipenem(100%). Conclusion: This study signifies that prevalence of aerobic bacteria, especially Pseudomonas aeruginosa and Klebsiella pneumonia was common in patients with post tubercular bronchiectasis. The Antibiotic resistance noted highest being Amikacin (56.4%) and sensitivity with imipenem(100%). In patients colonizing Pseudomonas aeruginosa, there was significant decline in lung function.

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Identifikasi dan Uji Resistensi Bakteri pada Pasien Ulkus Diabetikum di Bangsal Interne RSUP Dr. M. Djamil Padang

Jurnal Penelitian Farmasi Indonesia ◽

10.51887/jpfi.v8i2.550 ◽

2019 ◽

Vol 8 (2) ◽

pp. 67-74

Author(s):

Ringga Novelni

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Proteus Mirabilis ◽

Staphylococcus Epidermidis ◽

Klebsiella Pneumonia ◽

Proteus Vulgaris ◽

Diabetes Melitus

Ulkus diabetikum adalah salah satu komplikasi kronis diabetes melitus berupa luka terbuka pada permukaan kulit yang dapat disertai adanya kematian jaringan. Kadar glukosa darah yang tinggi menjadi tempat strategis perkembangan bakteri. Penelitian ini bertujuan untuk mengetahui bakteri penyebab ulkus diabetikum dan resistensinya terhadap antibiotik. Jenis penelitian ini adalah Deskriptif Eksploratif. Pada penelitian ini didapatkan 10 sampel pasien ulkus diabetikum, 4 sampel Klebsiella pneumonia, 2 sampel Staphylococcus epidermidis dan 1 sampel Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris dan Proteus mirabilis. Kemudian dilakukan identifikasi bakteri dan uji resisitensi antibiotik terhadap 5 jenis antibiotik dengan menggunakan metode difusi. Jenis bakteri yang banyak ditemukan dalam pus ulkus diabetikum berturur-turut adalah Klebsiella pneumonia (40%), Staphylococcus epidermidis (60%). Proteus vulgaris (10%), Proteus mirabilis (10%), Pseudomonas aeruginosa (10%) dan Staphylococcus aureus (10%). Ciprofloksasin dan cefotaxime menduduki peringkat tertinggi tingkat resistensi dengan jumlah resistensi bakteri terbanyak (40%), disusul Meropenem dan Gentamisin (30%), sedangkan Vankomisin efektif digunakan pada bakteri Staphylococcus aureus dan Staphylococcus epidermidis dengan tingkat resistensi (0%).

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Chicken feather hydrolysate as alternative peptone source for microbial cultivation

F1000Research ◽

10.12688/f1000research.17134.2 ◽

2019 ◽

Vol 7 ◽

pp. 1918

Author(s):

Oghenerobor B. Akpor ◽

Damilola E. Odesola ◽

Remilekun E. Thomas ◽

Olarewaju M. Oluba

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Proteus Mirabilis ◽

Protein Hydrolysate ◽

Test Organism ◽

Maximum Growth ◽

Chicken Feather ◽

E Coli ◽

Bacteria And Fungi

Background: Commercially available conventional growth medium for the culture of microbes are expensive, hence the need for alternative cheaper sources. Poultry waste, in the form of feather and blood, are of value in biotechnology because of their high protein content. Hence the primary aim of this study was to produce a cheaper peptone alternative from chicken feather protein hydrolysate (CFPH) and blood meal (BM). Methods: We monitored the growth of selected bacteria and fungi in different concentrations of medium produced from varying combination of peptone, CFPH and BM in order to determine the combination that produced maximum growth. Five different media, namely 100% peptone (control), 100% BM, 40% peptone + 60% CFPH, 40% BM + 60% CFPH and 20% peptone + 20% BM + 60% CFPH were prepared and used for the study. The different media were inoculated with 1 ml of each test organism (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida carpophila, Candida tropicalis and Pichia kundriavzevii) and their growth monitored for 10 h. Results: Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus grew best in the 100% peptone, Klebsiella pneumoniae grew best in 100 BM. The fungi species were observed to grow best in 100% peptone. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of trichloroacetic acid (TCA), hydrochloric acid (HCl) and nitic acid (HNO3) gave the best growth of E. coli. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of TCA) also gave the best growth of C. tropicalis and Klebsiella pneumoniae. Conclusions: Overall, the 60% CFPH + 40% peptone combination showed the most potential as an alternative to peptone, especially for E. coli.

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Μελέτη των παθογόνων μικροοργανισμών σε λοιμώξεις διαβητικού ποδιού

10.12681/eadd/41709 ◽

2017 ◽

Author(s):

Μαρία Δημητρίου

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Proteus Mirabilis ◽

Staphylococcus Epidermidis ◽

Klebsiella Pneumonia

Σκοπός της μελέτης ήταν η αξιολόγηση των πληροφοριών από την καλλιέργεια με βαμβακοφόρο στυλεό και την ποσοτική ιστική καλλιέργεια σε ασθενείς με νευροπαθητικό ή νευροϊσχαιμικό έλκος διαβητικού ποδιού και κλινική εικόνα λοίμωξης αυτού. Συμπεριελήφθησαν 113 ασθενείς, οι οποίοι χωρίστηκαν στην ομάδα Α (63 ασθενείς με νευροπαθητικό έλκος) και στην ομάδα Β (50 ασθενείς με νευροϊσχαιμικό έλκος). Η διάκριση του έλκους έγινε με βάση τα κλινικά χαρακτηριστικά αυτού και την κλινική εξέταση για τη διαπίστωση διαβητικής περιφερικής νευροπάθειας και περιφερικής αρτηριακής νόσου. Οι ασθενείς της ομάδας Β παρουσίαζαν σημαντικά μεγαλύτερη ηλικία και διάρκεια ΣΔ, όπως επίσης και σημαντικά βαρύτερη κλινική εικόνα περιφερικής νευροπάθειας και κλινική εικόνα λοίμωξης αλλά σημαντικά βραχύτερη διάρκεια έλκους ποδιού. Και στις δύο ομάδες, η καλλιέργεια με βαμβακοφόρο στυλεό για την ταυτοποίηση του υπεύθυνου παθογόνου παρουσίαζε υψηλή ευαισθησία (περίπου 90%) αλλά εξαιρετικά χαμηλή ειδικότητα (11-16%). Χάρη στην υψηλή ευαισθησία και τη χαμηλή τιμή λόγου αρνητικής πιθανοφάνειας (-LR), η καλλιέργεια με βαμβακοφόρο στυλεό αναδείχθηκε χρήσιμη για τον αποκλεισμό ενός μικροοργανισμού ως υπεύθυνου παθογόνου της λοίμωξης. Σε κάθε ομάδα, τα συχνότερα παθογόνα αποτέλεσαν ο Staphylococcus aureus και η Pseudomonas aeruginosa, ακολουθούμενα από την Klebsiella pneumonia, το Staphylococcus epidermidis και τον Proteus mirabilis, ενώ τα αναερόβια ήταν εξαιρετικά σπάνια. Επίσης σε κάθε ομάδα, ο αριθμός των απομονωθέντων ήταν σημαντικά υψηλότερος στο βαμβακοφόρο στυλεό σε σύγκριση με την ιστική καλλιέργεια. Αντίθετα δεν διαπιστώθηκε διαφορά μεταξύ των δύο ομάδων ως προς το μικροβιακό φορτίο (στην ποσοτική ιστική καλλιέργεια) αλλά ούτε και ως προς τον αριθμό των απομονωθέντων στελεχών σε οποιαδήποτε μέθοδο καλλιέργειας. Σε κάποιους ασθενείς απομονώθηκαν και λιγότερο συνήθη παθογόνα: η προηγούμενη λήψη αντιβιοτικών, οι συχνές επισκέψεις σε νοσοκομεία και η μακρά διάρκεια του έλκους (> 4 μήνες) αποτελούσαν τους κύριους παράγοντες κινδύνου για αυτά. Τέλος, η αντοχή των αντιβιοτικών στα παθογόνα γενικά δεν ήταν υψηλή. Η πιπερακιλλίνη-ταζομπακτάμη αποτέλεσε το αντιβιοτικό με τη χαμηλότερη αντοχή στην Ψευδομονάδα αλλά και γενικότερα στα Gram-αρνητικά παθογόνα. Πολύ χαμηλή αντοχή στα Gram-αρνητικά παθογόνα παρουσίασαν επίσης η κεφταζιδίμη, η σιπροφλοξασίνη και οι καρβαπενέμες. Στους Gram- θετικούς κόκκους διαπιστώθηκε υψηλή αντοχή της κλινδαμυκίνης και της ερυθρομυκίνης. Στους MRSA, MSSA και στους εντεροκόκκους δεν διαπιστώθηκε αντοχή στα αντισταφυλοκοκκικά (βανκομυκίνη, τεϊκοπλανίνη, λινεζολίδη και τιγεκυκλίνη). Στους MSSA διαπιστώθηκε επίσης πολύ καλή δραστικότητα της ριφαμπικίνης και της κοτριμοξαζόλης.

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Efeito antimicrobiano do extrato de Cranberry sobre micro-organismos causadores de infecção urinária

Cadernos UniFOA ◽

10.47385/cadunifoa.v11.n31.384 ◽

2016 ◽

Vol 11 (31) ◽

pp. 113-122

Author(s):

Carla Franco Porto Belmont Souza ◽

Luiz Eduardo Souza da Silva Irineu ◽

Renan Silva De Souza ◽

Renato da Silva Teixeira ◽

Ivina Sanches Pereira ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Enterococcus Faecalis ◽

Proteus Mirabilis ◽

Enterococcus Faecium ◽

Vaccinium Macrocarpon ◽

Mueller Hinton

A resistência microbiana tem se mostrado um problema de proporções mundiais, causando estado de morbidade e mortalidade em diversos pacientes. Em vista disso, tem crescido a busca por métodos alternativos naturais de profilaxia. A investigação clínica sugere que o Extrato de Cranberry está entre as melhores propostas de prevenção natural. O Cranberry (Vaccinium macrocarpon) é um fruto que tem crescido comercialmente pelo sabor e propriedades benéficas à saúde. Dentre as formas comercializadas estão: o suco, o chá e as cápsulas contendo o extrato seco. A ação desta planta está relacionada ao tratamento de doenças do trato urinário, por possuir substâncias que inibem a adesão bacteriana ao epitélio do trato urinário, dificultando sua proliferação e reprodução. Dentre todas as infecções relacionadas à assistência a saúde, a Infecção do Trato Urinário é a mais frequentemente associada a procedimentos invasivos. Se não for tratada, pode resultar em complicações como pielonefrite aguda, bacteremia e pionefrose. Portanto, cranberry pode ser uma nova alternativa para o combate das infecções uroepiteliais, por ser um produto natural de preço acessível, e com formas de comercialização diversificada, ao contrário dos antimicrobianos convencionais, que por sua vez são caros e podem acabar causando resistência nos micro-organismos. Este trabalho teve como objetivo avaliar in vitro a atividade antimicrobiana do extrato de Cranberry, adquirido em farmácia de manipulação, sobre 8 micro-organismos isolados de infecções urinárias. As cepas utilizadas, adquiridas da coleção da FIOCRUZ, foram: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Serratia marscecens, Staphylococcus aureus, Enterococcus faecalis e Enterococcus faecium. No estudo, foram utilizados o caldo Mueller Hinton (MH), Extrato de Cranberry e as bactérias patogênicas. O ensaio foi realizado em triplicata, com o uso de um controle de crescimento dos micro-organismos e o experimento para avaliação do crescimento bacteriano na presença do extrato. A turbidez foi medida com o auxílio de um espectrofotômetro, no comprimento de onda de 600 nm, antes e após 24 horas de incubação à 37 ºC. O procedimento forneceu a Densidade Ótica, do qual possibilitou a identificação da inibição microbiana. Para análise estatística foi utilizado o Teste t de Student. O Extrato de Cranberry apresentou atividade antimicrobiana sobre as bactérias Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Serratia marscecens e Enterococcus faecalis (p < 0,05), confirmando seu efeito benéfico em infecções urinárias. No entanto, não teve efeito inibitório significativo sobre Pseudomonas aeruginosa, Proteus mirabilis e Enterococcus faecium (p > 0,05).

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Improvement of the Efficacy an WHO Alcohol-based Hand Rub Formulation by Reducing Isopropanol Content and Associating Syzygium aromarticum and Piper nigrum Essential Oils

Journal of Applied Life Sciences International ◽

10.9734/jalsi/2019/v21i330105 ◽

2019 ◽

pp. 1-11

Author(s):

Mahamat Ismail ◽

Veronique Penlap ◽

Wilfred F. Mbacham ◽

Sylvain Leroy Sado Kamdem

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Essential Oils ◽

Salmonella Typhi ◽

World Health ◽

Partial Substitution ◽

Piper Nigrum ◽

Panel Test ◽

One Year

Aim: This work was carried out in order to reduce the isopropanol contain of a World Health Organization (WHO) basic alcohol based hand rub (ABHR) formulation by substituting part of it with ethanol and essential oils. Study Design: A quasi-experimental design was used, based on WHO basic formulation modification, antimicrobial assessment followed by a panel test and challenge test of the best formulation. Place and Duration of the Study: The study was carried out in different laboratory of the University of Yaoundé 1 during March 2017 to July 2018. Methodology: This study was performed by producing different formulations nested from the WHO basic formulation and testing their antimicrobial capacity on selected strains based on the microbial percentage reduction. Following this, the best formulation was compared to two commercial products trough a panel test and later challenged with selected organism (Staphylococcus aureus SR196, Salmonella Typhi 15SA, Escherichia coli ATTC25922 and Pseudomonas aeruginosa PA01) inoculated in the product after different storage periods for one year and the antimicrobial stability assessed on the same strain for the same length of conservation. Results: The partial substitution of isopropanol with ethanol and Piper nigrum and Syzygium aromaticum essential oils proved to possess more antimicrobial properties than the original WHO formulation. The best formulation caused a 6 Log cells/ml reduction of the initial population compared to the 4 Log cells/ml of the WHO formulation. The product also proved to maintain its activity for one year and to be able to deactivate possible contaminations by Salmonella Typhi 15SA, Staphylococcus aureus NCTC10652, Escherichia coli ATCC25922, Pseudomonas aeruginosa PA01. Conclusion: The present work is a contribution to the improvement of ABHRs and could permit the reduction of hand hygiene associated infections in industries and health care facilities.

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Isolamento e teste de susceptibilidade a antimicrobianos de bactérias em infecções uterinas de éguas

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09351999000300002 ◽

1999 ◽

Vol 51 (3) ◽

pp. 213-216 ◽

Cited By ~ 5

Author(s):

N. Silva ◽

C.E. Braga ◽

G.M. Costa ◽

F.C.F. Lobato

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Proteus Mirabilis ◽

Rhodococcus Equi ◽

Minas Gerais ◽

Streptococcus Equi ◽

Bacillus Spp

Foram examinados 206 "swabs" cervicais e uterinos de éguas de várias raças, de diversas regiões do Estado de Minas Gerais, durante o período de 1986 a 1996. Cerca de 164 "swabs" foram positivos para a presença de microrganismos causadores de endometrites. Streptococcus equi subsp. zooepidemicus (25,7%) e Escherichia coli (15,1%) foram os principais agentes infecciosos isolados. Outros microrganismos presentes foram: Staphylococcus aureus (9,2%), Streptococcus alfa-hemolítico (9,2%), Pseudomonas aeruginosa (3,9%), Staphylococcus coagulase negativo (6,3%), Bacillus spp. (1,9%), Rhodococcus equi (3,4%) e Proteus mirabilis (1,5%). As provas de susceptibilidade aos antimicrobianos revelaram que amicacina e gentamicina (70,2%), ampicilina (59,5%) e cloranfenicol (59,5%) foram os antibióticos de maior ação in vitro contra os microrganismos isolados.

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Pola Kuman dan Uji Kepekaan pada Pasien Community Acquired Pneumonia di RSUP Dr. M. Djamil Padang Tahun 2016

Jurnal Kesehatan Andalas ◽

10.25077/jka.v8.i3.p553-557.2019 ◽

2019 ◽

Vol 8 (3) ◽

pp. 553

Author(s):

Narlis Narlis ◽

Ellyza Nasrul ◽

Efrida Efrida

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Staphylococcus Epidermidis ◽

Community Acquired Pneumonia ◽

Klebsiella Pneumonia ◽

Proteus Vulgaris ◽

Streptococcus Pneumonia

Community acquired pneumonia (CAP) merupakan infeksi yang paling sering menyebabkan sepsis dan dapat menimbulkan kematian. Pertimbangan pemilihan antimikroba yang tepat dan menghindari penggunaan yang berlebihan perlu dilakukan untuk mencegah terjadinya resistensi. Tujuan penelitian ini adalah mengetahui pola kuman dan uji kepekaan pasien CAP di RSUP Dr. M. Djamil Padang. Penelitian ini merupakan penelitian deskriptif retrospektif terhadap 201 sampel sputum pasien CAP yang diperiksa kultur kemudian dilanjutkan dengan pewarnaan Gram dan uji biokimia untuk mengidentifikasi bakteri. Uji kepekaan antimikroba menggunakan metode difusi cakram. Penelitian dilakukan di Laboratorium Sentral RSUP Dr. M. Djamil Padang periode Januari 2016 hingga Desember 2016. Data ditampilkan dalam bentuk tabel distribusi frekuensi. Mikroorganisme yang ditemukan adalah Klebsiella pneumonia (55,23%), Staphylococcus aureus (25,87%), Streptococcus pneumonia (7,96%), Pseudomonas aeruginosa (5,97%), Staphylococcus epidermidis (2,9%), Acinetobacter baumani (1,99%), Proteus vulgaris (0,49%). Antimikroba yang paling sensitif adalah Meropenem (78,7%), sedangkan antimikroba resisten dengan persentase tertinggi adalah Ampisilin (90%), Amoksisilin (89,4%), Eritromisin (73,5%), Amoksisilin + Klavulanat (64,6%), dan Kloramfenikol (61,7%). Mikroorganisme yang paling banyak ditemukan adalah Klebsiella pneumonia (55,23%). Meropenem merupakan antimikroba sensitif dengan persentase paling tinggi (78,7%) sedangkan antimikroba resisten dengan persentase paling tinggi adalah ampisilin (90%).

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