Screening for anti-inflammatory activity of extract and fractions of Morinda lucida stem bark and detection of bioactive secondary metabolite

Morinda lucida Benth (Rubiaceae) is a versatile plant used in traditional medicine for the treatment of a variety of ailments and the claims of its efficacy are particularly remarkable in the treatment of infections and immuno-inflammatory disorders. This study evaluates the anti-inflammatory properties of methanolic stem bark extract and fractions of M. lucida and also identifies the phytochemicals responsible for its anti-inflammatory activity. The crude extract was subjected to liquid- liquid partitioning successively with n- hexane, ethylacetate, butanol and water. High performance liquid chromatography (HPLC) of the four fractions and Vacuum Liquid Chromatography fraction (VLC) of the promising fraction was evaluated. The effect of the fractions on egg albumen induced rat paw oedema were also evaluated. Anti-inflammatory activity of the fractions was further screened using xylene induce ear oedema models and human red blood cell membrane stabilization test. Ulcerogenic test on the normal stomach mucosa was also evaluated. The result of the egg albumen induced rat paw oedema showed that the butanol fractions maximally inhibited egg albumen induced effect at 400 mg/kg (70%) and 200mg/kg (67.5%) after 180 minutes compared to the positive control, ibuprofen (20mg/kg) with 100% inhibition after 180 minutes. The result of the xylene induced ear oedema showed that the inhibition produced by 100 µg/ear of the Butanol fraction (BF) was 56.67 % and was greater than inhibition produced by 200 µg/ear of ibuprofen (38.89 %). HPLC analysis of the fractions revealed the following phytocompounds; Cytreo- a-pyrone, Cytosporin- J and Waol A. Ulcerogenic test was negative at the doses of 200 mg/kg and 400 mg/kg of the fractions when compare with the indomethacin (positive control) at dose of 50 mg/kg. Human red blood cell membrane stabilization assay showed that BF-VLC 2 (Dichloromethane: methanol (8:2) VLC of Butanol fraction) exhibited concentration dependent inhibition of heat-induced haemolysis while other extract showed a non- concentration dependent inhibition of haemolysis when compared to the standard, ibuprofen. These findings suggest that the stem bark of M. lucida possess promising anti-inflammatory phytocompounds which justify its use in ethno-medicine.

Download Full-text

Anti-Inflammatory Activity of Pleurotus ostreatus, a Culinary Medicinal Mushroom, in Wistar Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/6845383 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

W. J. A. Banukie N. Jayasuriya ◽

Shiroma M. Handunnetti ◽

Chandanie A. Wanigatunge ◽

Gita H. Fernando ◽

D. Thusitha U. Abeytunga ◽

...

Keyword(s):

Pleurotus Ostreatus ◽

Inflammatory Activity ◽

No Production ◽

Protective Effects ◽

Paw Oedema ◽

Freeze Dried ◽

Anti Inflammatory ◽

Rat Paw ◽

Rat Paw Oedema

Context. Pleurotus ostreatus (P.o) is a culinary mushroom which is commonly called as “oyster mushroom” belonging to the Basidiomycetous fungi of the order Agaricales and family Pleurotaceae. Objectives. The present study investigates the anti-inflammatory potential of P.o and the underlying mechanisms of activity. Materials and Methods. Anti-inflammatory activity was evaluated using suspensions of freeze-dried and powdered (SFDP) P.o and acetone extract (AE) of P.o in normal and alloxan-induced diabetic rats using the carrageenan-induced rat paw oedema model. The mechanisms by which P.o is mediating the anti-inflammatory activity were studied using in vivo and in vitro assays. Results. At doses of 500–1000 mg/kg, the SFDP of P.o showed long-lasting activity at both early and late phases of carrageenan-induced rat paw oedema. The dose of 750 mg/kg showed the most potent inhibitory activity (92% inhibition) in healthy rats. The AE of P.o showed maximum inhibition of oedema of 87%. P.o exerted protective effects on the inflammatory pathologies in rats with diabetes. The possible mechanisms by which P.o mediates the anti-inflammatory activity were antihistamine activity (52.1%), inhibition of cell migration to the site of inflammation (45.4%), in vitro membrane stabilizing activity (52.6%), and inhibition of nitric oxide (NO) production (91.2%) (P<0.05). Dose-dependent inhibition of NO production was seen with in vitro treatment of rat peritoneal cells with AE of P.o (r = 0.95; P<0.05). Discussion and Conclusion. The promising activity of culinary mushroom P.o against inflammation suggests its potential application as a functional food during inflammatory conditions.

Download Full-text

Anti-inflammatory activity of Syzygium cumini leaf against experimentally induced acute and chronic inflammations in rodents

Alternative Medicine Studies ◽

10.4081/ams.2011.e6 ◽

2011 ◽

Vol 1 (1) ◽

pp. 6 ◽

Cited By ~ 5

Author(s):

Anirban Roy ◽

Sanjib Bhattacharya ◽

Jitendra N. Pandey ◽

Moulisha Biswas

Keyword(s):

Inflammatory Activity ◽

Dependent Manner ◽

Syzygium Cumini ◽

Large Size ◽

Paw Oedema ◽

Chronic Models ◽

Anti Inflammatory ◽

Rat Paw ◽

Dose Dependent ◽

Rat Paw Oedema

Syzygium cumini (L.) Skeels (Myrtaceae), commonly known as Jam in Bengali, Jamun in Hindi and Black Plum or Black Berry in English, is a large size evergreen tree indigenous to India and is cultivated for its fruits. In the present study, the methanol extract of leaves from S. cumini (MESC) was evaluated for anti-inflammatory activity in experimental acute (carrageenan, histamine and serotonin induced rat paw oedema) and chronic models (cotton pellet induced rat granuloma). In all models, the MESC (100 and 200 mg/kg body wt. p.o.) exhibited significant anti-inflammatory activity (p < 0.001) in a dose dependent manner. These findings revealed that the S. cumini leaf had remarkable acute and chronic anti-inflammatory actions in the tested rodent models.

Download Full-text

Synthesis, characterization and pharmacological evaluation of substituted phenoxy acetamide derivatives

Hemijska industrija ◽

10.2298/hemind140330057r ◽

2015 ◽

Vol 69 (4) ◽

pp. 405-415 ◽

Cited By ~ 1

Author(s):

Priyanka Rani ◽

Dilip Pal ◽

Rahul Hegde ◽

Syed Hashim

Keyword(s):

Analgesic Activity ◽

Diclofenac Sodium ◽

Inflammatory Activity ◽

The Novel ◽

Standard Drug ◽

Antipyretic Activity ◽

Paw Oedema ◽

Anti Inflammatory ◽

Rat Paw ◽

Rat Paw Oedema

A novel series of 2-(substituted phenoxy)-N-(1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)acetamide and N-(2-bromocyclohexyl)-2-(substituted phenoxy)acetamide derivatives having cyclohexyl nucleus as common in both types were synthesized and assessed for their anti-inflammatory activity by a carrageenan induced rat paw oedema method, analgesic activity by Eddy?s hot plate method and antipyretic activity by brewer?s yeast induced pyrexia method. All the novel derivatives have been synthesized by the reaction of camphor and similar ketone having cyclohexane nucleus (e.g. 2-bromocyclohexanone) with ammonium carbonate and formic acid resulting in the formation of aromatic amines (1a-b). These amines on further chloroacetylation with chloroacetylchloride give compounds (2a-b). Compounds (2a-b) are converted to 2-(substituted phenoxy)-N-(1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) acetamide and N-(2-bromocyclohexyl)-2-(substituted phenoxy)acetamide derivatives on treatment with substituted phenol. Among the series 3a-f, 3i, 3k, 3l compounds showed significant anti-inflammatory activity as compared to the standard drug diclofenac sodium and also compound 3a-f, 3h, 3j, 3k exhibit significant analgesic activity as compared to the standard drug. Compounds 3a-f and 3k showed antipyretic activity nearly to the standard drug indomethacin. Compounds 3a-f and 3k possess anti-inflammatory, analgesic and antipyretic activities near to the standard.

Download Full-text

ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT OF PLANT SIDA CORDATA IN CARRAGEENAN-INDUCED PAW EDEMA IN RATS

Journal of Pharmaceutical and Scientific Innovation ◽

10.7897/2277-4572.102205 ◽

2021 ◽

Vol 10 (2) ◽

pp. 53-55

Author(s):

Prajakta P Shinde ◽

Shahu D Khule ◽

Sneha Sonawane ◽

Suvarna Shelke

Keyword(s):

Methanolic Extract ◽

White Blood Cells ◽

Inflammatory Activity ◽

Clinical Practices ◽

Standard Drug ◽

Paw Oedema ◽

Ancient Times ◽

Anti Inflammatory ◽

Rat Paw ◽

Rat Paw Oedema

Since ancient times in India several medicinal plants are in use for clinical practices. Inflammation is a process through which the body's white blood cells and the things they make protect body from infection from outside invaders, such as bacteria and viruses. Currently many of the plant’s species have been reported effective as an Anti-inflammatory, but only a few of them have been investigated. Currently many drugs are available to control the inflammation but has several adverse effects. The plant Sida Cordata was reported to have several activities such as Antioxidant, Analgesic, Antidepressant, Antihyperglycemic, Hepatoprotective Antibacterial, Antitumor, Antifungal, Antiulcer, Antitussive, Anti-inflammatory, Anti-malarial, in ayurvedic system of medicine. This study was undertaken to investigate the anti-inflammatory activity of Methanolic extract of Sida Cordata. The methanolic extract of Sida Cordata used for treatment of Carrageenan-induced Rat paw Oedema model with the predefined dose as reported. The experimental groups with higher dose of methanolic extract (200 mg/kg) exhibited a significant anti-inflammatory effect after 2 hr. (p<0.01); based on the observation, the activity was favorable, which is nearly equal to that of the standard drug Indomethacin (10 mg/kg). The methanol extract (200 mg/kg) shows the maximum percentage of inhibition of inflammation 27.06 %. The standard drug indomethacin shows 33 % inhibition.

Download Full-text

Fibrinolytic, Anti-Inflammatory and Cytotoxic Potentialities of Extracts and Chemical Constituents of Manglicolous Lichen, Graphis ajarekarii Patw. & C. R. Kulk

The Natural Products Journal ◽

10.2174/2210315508666180604101813 ◽

2020 ◽

Vol 10 (1) ◽

pp. 87-93 ◽

Cited By ~ 1

Author(s):

Vinay Bharadwaj Tatipamula ◽

Girija Sastry Vedula

Keyword(s):

Protein Denaturation ◽

Biological Activities ◽

Chemical Constituents ◽

Fibrin Clot ◽

Hydroalcoholic Extract ◽

Paw Oedema ◽

Anti Inflammatory ◽

Rat Paw ◽

Rat Paw Oedema

Background: Lichens which are betide to mangroves are termed as Manglicolous Lichens (ML). As these ML are habituated under stress conditions, they are screened for unique metabolites and biological activities. Objective: The study aimed to establish the chemical and biological profile of ML, Graphis ajarekarii. Methods: The Ethyl Acetate Extract of G. ajarekarii (EAE) was subjected to chromatographic techniques and the obtained isolates were characterized by spectroscopic analysis. The hydroalcoholic extract of G. ajarekarii (AE), EAE, isolates and Hydroalcoholic Extract of host (HE) were evaluated for fibrinolytic (fibrin clot method), in vitro (protein denaturation method) and in vivo (formalin-induced rat paw oedema assay), anti-inflammatory and cytotoxicity (MTT assay) activities. Results: Chemical investigation of the EAE led to the isolation of two known compounds namely atranorin (1) and ribenone (2), which were confirmed by spectral data. The AE and EAE gradually lysed the fibrin clot with 94.54 and 65.07%, respectively, at 24 h. The AE inhibited protein denaturation of about 88.06%, while the standard (Indomethacin) with 93.62%. Similarly, the in vivo antiinflammatory analysis of AE (200 mg/mL) showed potent reduction of rat paw oedema than the standard, whereas EAE and 1 depicted moderate depletion. In addition, the AE revealed prominence inhibition on MCF-7, DU145 and K-562 with IC50 values of 69.5, 42.5 and 38 µg/mL, respectively, whereas the HE exhibited mild inhibitory profile against fibrin clot, inflammation and cancer. Conclusion: From the results, it can be concluded that the G. ajarekarii has an aptitude to act against coagulation, inflammation and cancer cells.

Download Full-text

Anti-Inflammatory Constituents of Alchornea cordifolia Leaves

Nigerian Journal of Natural Products and Medicine ◽

10.4314/njnpm.v19i0.5 ◽

2015 ◽

Vol 19 ◽

pp. 60-64

Author(s):

AA Ahmadu ◽

A Agunu ◽

EM Abdurrahman

Keyword(s):

Medicinal Plant ◽

West Africa ◽

Ethyl Acetate ◽

Leaf Extract ◽

Methyl Gallate ◽

Paw Oedema ◽

Analgesic Agents ◽

Anti Inflammatory ◽

Rat Paw ◽

Rat Paw Oedema

Alchornea cordifolia (Schum and Thonn) Muell. Arg. (Euphorbiaceae) is a traditional medicinal plant widely distributed in West Africa including Nigeria.The plant has been used for ethnomedicinal purposes against wounds, ulcers, and sores. The decoction of the leaves has been reported to provide remedies for bronchial problems, rheumatic pain and cough. From the dichloromethane and ethyl acetate soluble parts of the Methanol leaf extract, two compounds namely Lup-20(29)-en-3c-ol (lupenol) and Methyl 3, 4,5-trihydroxy benzoate (Methyl gallate) were isolated and their structures elucidated. Anti-inflammatory and analgesic properties of the compounds on carrageenan-induced paw oedema and formalin-induced pain in rats showed that compound 2 significantly (P<0.05) inhibit rat paw oedema compared to the standard drugs (Piroxicam and Morphine) used, while on formalin-induced pain in rats, the same trend was observed and were both comparable to Piroxicam and morphine, the standard anti-inflammatory and analgesic agents used, respectively. Compound 1 did not show any significant anti-inflammatory activity compared to control, likewise compound 2. Thus, compound 2, Methyl trihydroxy benzoate, might be responsible for the anti-inflammatory and analgesic properties of this plant.Keywords: Alchornea cordifolia, Anti-inflammatory, Analgesic, Methyl Trihydroxy Benzoate

Download Full-text

A New Cytotoxic Sesquiterpene and Three Anti-inflammatory Flavonoids from Egyptian Tanacetum Santolinoides

Natural Product Communications ◽

10.1177/1934578x0700201102 ◽

2007 ◽

Vol 2 (11) ◽

pp. 1934578X0700201 ◽

Cited By ~ 1

Author(s):

Sabrin R. M. Ibrahim ◽

Jihan M. Badr ◽

Khalid A. El Sayed ◽

Diaa T. A. Youssef

Keyword(s):

Cytotoxic Activity ◽

Brine Shrimp ◽

Methanolic Extract ◽

Spectral Characteristics ◽

Mass Spectral ◽

Aerial Parts ◽

Paw Oedema ◽

Anti Inflammatory ◽

Rat Paw ◽

Rat Paw Oedema

A re-investigation of the methanolic extract of the aerial parts of Tanacetum santolinoides has resulted in the isolation of a new sesquiterpene, tanacetonic acid (1), along with three known methoxylated flavonoids. The structure of the isolated compounds was secured based on their IR, UV, 1D and 2D (1H-1H COSY, HSQC and HMBC) NMR spectroscopic and FAB mass spectral characteristics. The new sesquiterpene showed a significant cytotoxic activity when tested using the brine shrimp bioassay, while the three flavonoids demonstrated anti-inflammatory effects in the rat paw oedema test.

Download Full-text

Synthesis and Evaluation of New Coumarin Derivatives as Antioxidant, Antimicrobial, and Anti-Inflammatory Agents

Molecules ◽

10.3390/molecules25143251 ◽

2020 ◽

Vol 25 (14) ◽

pp. 3251 ◽

Cited By ~ 1

Author(s):

Hanan M. Alshibl ◽

Ebtehal S. Al-Abdullah ◽

Mogedda E. Haiba ◽

Hamad M. Alkahtani ◽

Ghada E.A. Awad ◽

...

Keyword(s):

Significant Inhibition ◽

Coumarin Derivatives ◽

Paw Oedema ◽

Cox 2 ◽

Anti Inflammatory ◽

Rat Paw ◽

Orally Active ◽

Rat Paw Oedema ◽

Sulfonamide Compound

New pyranocoumarin and coumarin-sulfonamide derivatives were prepared and evaluated for their antioxidant, antimicrobial, and/or anti-inflammatory activities. Coumarin-sulfonamide compounds 8a–d demonstrated significant antioxidant activity, while 7c,d, 8c,d, and 9c,d exhibited antimicrobial activity equal to or higher than the standard antimicrobials against at least one tested microorganism. Regarding the anti-inflammatory testing, pyranocoumarins 2b, 3a,b and 5c and coumarin-sulfonamide compound 9a showed more potent antiproteinase activity than aspirin in vitro; however, five compounds were as potent as aspirin. The anti-inflammatory activity of the promising compounds was further assessed pharmacologically on formaldehyde-induced rat paw oedema and showed significant inhibition of oedema. For in vitro COX-inhibitory activity of coumarin derivatives, pyranocoumarin derivative 5a was the most selective (SI = 152) and coumarin-sulfonamide derivative 8d was most active toward COX-2 isozyme. The most active derivatives met the in silico criteria for orally active drugs; thus, they may serve as promising candidates to develop more potent and highly efficient antioxidant, antimicrobial, and/or anti-inflammatory agents.

Download Full-text

Anti-inflammatory and Anti-arthritic Activities ofMerremia tridentate(L.) Hall. f.

E-Journal of Chemistry ◽

10.1155/2009/670617 ◽

2009 ◽

Vol 6 (4) ◽

pp. 943-948 ◽

Cited By ~ 7

Author(s):

M. Kamalutheen ◽

S. Gopalakrishnan ◽

T. Syed Ismail

Keyword(s):

Ethanol Extract ◽

Albino Rats ◽

Standard Drug ◽

Arthritis Model ◽

Paw Oedema ◽

Dependent Activity ◽

Anti Inflammatory ◽

Rat Paw ◽

Dose Dependent ◽

Rat Paw Oedema

The various extracts ofMerremia tridentatewere investigated for its anti-inflammatory and anti-arthritic activities in male albino rats. The anti-inflammatory activity was carried out using carrageenan-induced rat paw oedema model and the anti-arthritic activity was carried out using complete Freund’s adjuvant induced arthritis model. Indomethacin (10 mg/kg bw) was used as a standard drug. The ethanol extract ofM. tridentateexhibited significant dose dependent activity in acute inflammation and the doses of 100 mg/kg bw and 200 mg/kg bw produced 38.3% and 42.8% inhibition respectively after 3 h as compared with that of the standard drug which showed 48.5% inhibition. In arthritis model, the doses of 100 mg/kg bw and 200 mg/kg bw of the ethanol extract produced 49.0% and 51.7% inhibition respectively after 19 days when compared with that of the standard drug (55.5%). Both doses of the ethanol extract ofM. tridentateexhibit significant anti-inflammatory and anti-arthritic activities.

Download Full-text