Effect of moringa leaf meal and season on blood and hormonal profile of the pearl guinea fowl (Numida meleagris)

This study was undertaken to determine the effect of moringa leaf meal and season on blood and hormonal profile of the Pearl Guinea fowl (Numida meleagris) in the Middle belt of Ghana. Thirty-two (32) males and one hundred and twenty-eight (128) female Pearl Guinea fowls aged one-day-old were used for the study. A 3 x 4 factorial experimental design was used for the experiment. Data collected were analyzed using General Linear Model (GLM) procedure of Statistical Analysis System (SAS for Windows, version 7) and means were separated by the probability of difference (PDIFF) procedure of SAS (2008). Mean cell volume was highest (175.39 fl) among Guinea fowls fed with diet containing 12 % moringa leaf meal level. Guinea fowls fed with diet containing 15 % moringa leaf meal had the highest (3.44 x1012/L) red blood cells production. Eosinophil level was highest (3.95 x1012/L) among Guinea fowls fed with diet containing 9 % moringa leaf meal. Birds fed with the moringa diets recorded the highest (P= 0.022) WBC values as compared to the control diet. Triglycerides, high density lipoprotein and low-density lipoprotein levels increased (P<0.05) with increasing levels of dietary moringa leaf meal in the diet. The highest (P= 0.0025) level of progesterone was observed among birds fed with diet containing 12 and 15 % moringa leaf meal inclusion levels. The level of sodium was highest (166.69 nmol/l) among Guinea fowls fed with diet containing 12 % moringa leaf meal. The major and minor rainy seasons recorded the highest (P<0.05) mean cell hemoglobin, red blood cells, albumin and oestrogen levels. Platelets, follicle stimulating hormone, luteinizing hormone, prolactin and chlorine levels were highest (P<0.05) in the dry season while basophil level was highest in the major rainy season. Cholesterol, triglycerides, high density lipoprotein, low density lipoprotein and potassium levels were highest (P<0.05) in the major rainy season. This study concludes that feeding Guinea fowls with moringa leaf meal had positive effect on some haematological, biochemical and hormonal parameters.

Download Full-text

Engineered red blood cells carrying PCSK9 inhibitors persistently lower LDL and prevent obesity

PLoS ONE ◽

10.1371/journal.pone.0259353 ◽

2021 ◽

Vol 16 (11) ◽

pp. e0259353

Author(s):

Rhogerry Deshycka ◽

Valentino Sudaryo ◽

Nai-Jia Huang ◽

Yushu Xie ◽

Liyan Y. Smeding ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Chimeric Protein ◽

Mouse Bone Marrow ◽

Glycophorin A ◽

Low Density ◽

Hematopoietic Stem

Low plasma levels of Proprotein Convertase Subtilisin/Kexin 9 (PCSK9) are associated with decreased low-density lipoprotein (LDL) cholesterol and a reduced risk of cardiovascular disease. PCSK9 binds to the epidermal growth factor-like repeat A (EGFA) domain of LDL receptors (LDLR), very low-density lipoprotein receptors (VLDLR), apolipoprotein E receptor 2 (ApoER2), and lipoprotein receptor–related protein 1 (LRP1) and accelerates their degradation, thus acting as a key regulator of lipid metabolism. Antibody and RNAi—based PCSK9 inhibitor treatments lower cholesterol and prevent cardiovascular incidents in patients, but their high-cost hampers market penetration. We sought to develop a safe, long-term and one-time solution to treat hyperlipidemia. We created a cDNA encoding a chimeric protein in which the extracellular N- terminus of red blood cells (RBCs) specific glycophorin A was fused to the LDLR EGFA domain and introduced this gene into mouse bone marrow hematopoietic stem and progenitor cells (HSPCs). Following transplantation into irradiated mice, the animals produced RBCs with the EGFA domain (EGFA-GPA RBCs) displayed on their surface. These animals showed significantly reduced plasma PCSK9 (66.5% decrease) and reduced LDL levels (40% decrease) for as long as 12 months post-transplantation. Furthermore, the EGFA- GPA mice remained lean for life and maintained normal body weight under a high-fat diet. Hematopoietic stem cell gene therapy can generate red blood cells expressing an EGFA—glycophorin A chimeric protein as a practical and long-term strategy for treating chronic hyperlipidemia and obesity.

Download Full-text

Macrophages lacking scavenger receptor A show a decrease in binding and uptake of acetylated low-density lipoprotein and of apoptotic thymocytes, but not of oxidatively damaged red blood cells

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.94.15.8127 ◽

1997 ◽

Vol 94 (15) ◽

pp. 8127-8131 ◽

Cited By ~ 52

Author(s):

V. Terpstra ◽

N. Kondratenko ◽

D. Steinberg

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Low Density Lipoprotein ◽

Scavenger Receptor ◽

Density Lipoprotein ◽

Low Density ◽

Scavenger Receptor A

Download Full-text

Engineered Red Blood Cells Carrying PCSK9 Inhibitors Persistently Lower LDL and Prevent Obesity

10.1101/2020.11.02.364620 ◽

2020 ◽

Author(s):

Rhogerry Deshycka ◽

Valentino Sudaryo ◽

Nai-Jia Huang ◽

Yushu Xie ◽

Liyan Y. Smeding ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Chimeric Protein ◽

Mouse Bone Marrow ◽

Glycophorin A ◽

Low Density ◽

Hematopoietic Stem

AbstractLow plasma levels of Proprotein Convertase Subtilisin/Kexin 9 (PCSK9) are associated with decreased low-density lipoprotein (LDL) cholesterol and a reduced risk of cardiovascular disease. PCSK9 binds to the epidermal growth factor-like repeat A (EGFA) domain of LDL receptors (LDLR), very low-density lipoprotein receptors (VLDLR), apolipoprotein E receptor 2 (ApoER2), and lipoprotein receptor–related protein 1 (LRP1) and accelerates their degradation, thus acting as a key regulator of lipid metabolism. Antibody and RNAi - based PCSK9 inhibitor treatments lower cholesterol and prevent cardiovascular incidents in patients, but their high cost hampers market penetration. We sought to develop a safe, long-term and one-time solution to treat hyperlipidemia. We created a cDNA encoding a chimeric protein in which the extracellular N-terminus of glycophorin A was fused to the LDLR EGFA domain and introduced this gene into mouse bone marrow hematopoietic stem and progenitor cells (HSPCs). Following transplantation into irradiated mice, the animals produced red blood cells (RBCs) with the EGFA domain (EGFA-GPA RBCs) displayed on their surface. These animals showed significantly reduced plasma PCSK9 (66.5% decrease) and reduced LDL levels (40% decrease) for as long as 12 months post-transplantation. Furthermore, the EGFA-GPA mice remained lean for life and maintained normal body weight under high-fat diet. Hematopoietic stem cell gene therapy can generate red blood cells expressing an EGFA - glycophorin A chimeric protein as a practical and long-term strategy for treating chronic hyperlipidemia and obesity.

Download Full-text

Studies on the Thromboplastic Effect of Human Plasma Lipoproteins

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647942 ◽

1976 ◽

Vol 35 (01) ◽

pp. 178-185 ◽

Cited By ~ 8

Author(s):

Helena Sandberg ◽

Lars-Olov Andersson

Keyword(s):

High Density Lipoprotein ◽

Human Plasma ◽

Platelet Rich Plasma ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Plasma Lipoproteins ◽

Very Low Density Lipoprotein ◽

Low Density ◽

Free Plasma ◽

Good Agreement

SummaryHuman plasma lipoprotein fractions were prepared by flotation in the ultracentrifuge. Addition of these fractions to platelet-rich, platelet-poor and platelet-free plasma affected the partial thromboplastin and Stypven clotting times to various degrees. Addition of high density lipoprotein (HDL) to platelet-poor and platelet-free plasma shortened both the partial thromboplastin and the Stypven time, whereas addition of low density lipoprotein and very low density lipoprotein (LDL + VLDL) fractions only shortened the Stypven time. The additions had little or no effect in platelet-rich plasma.Experiments involving the addition of anti-HDL antibodies to plasmas with different platelet contents and measuring of clotting times produced results that were in good agreement with those noted when lipoprotein was added. The relation between structure and the clot-promoting activity of various phospholipid components is discussed.

Download Full-text