scholarly journals Anti-cancer, antiproliferative activity of active anionic H2O8 oxygen solution on HCT-116 cancer cell

2021 ◽  
Vol 12 (2) ◽  
pp. 179-184
Author(s):  
Apaydin Yildirim Betul
Keyword(s):  
Epithelial Cells ◽  
Colorectal Carcinoma ◽  
Cell Line ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Inflammatory Responses ◽  
Colorectal Cancer Cell Line ◽  
Hct 116 ◽  
Oxygen Solution ◽  
Human Colorectal Carcinoma

HCT116 cells are adherent epithelial cells derived from the human colorectal carcinoma cell line commonly used to study inflammatory responses in colonic epithelial cells. In this study, it was aimed to evaluate the effects of active anionic H2O8 oxygen solution, which is a very strong antiviral and antimicrobial agent, on HCT-116 human colorectal cancer cell line. Cell viability was determined by MTT analysis. Antiproliferative activity of the anionic H2O8 was investigated on HCT 116 (human colorectal carcinoma) cancer cells. Anionic H2O8 displayed the outstanding activities for MTT test, IC50= 9.44 for 24th hour was calculated as IC50= 11.73 for 48th hour on HCT 116 cell line. It is thought that it can serve as an agent with strong potential to be used in treatment.

scholarly journals Anti-migration Effect of Aaptos suberitoides Fraction in HCT-116 Colorectal Cancer Cell Line

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Dany Muhammad Daffa ◽  
Muhammad Hasan Bashari ◽  
Eko Fuji Ariyanto ◽  
Tenny Putri ◽  
Nurul Qomarilla
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cellular Migration ◽  
Colorectal Cancer Cell Line ◽  
Migration Inhibition ◽  
Migration Activity ◽  
Migration Effect ◽  
Hct 116

Background: Colorectal cancer is the second leading cause of mortality and the most prevalent cancer worldwide. Most patients, who come with late-stage, have ineffective treatments and some side effects in chemotherapy. Aaptos suberitoides has potential anti-cancer effects due to its bioactive compounds such as aptamine. This study aimed to evaluate the migration inhibition effect of Aaptos suberitoides fraction in HCT-116 cell line.Methods: This study was an experimental study. Aaptos suberitoides specimen was taken in Tinjil Island and fractionated with ethyl acetate. HCT-116 cell line was added with Aaptos suberitoides fraction and cellular migration activity was observed in 48 hours of which the scratch assay was performed. The gap closure area was determined with ImageJ software.Results: The data showed that a low concentration of Aaptos suberitoides fraction inhibited migration activity in HCT-116 cell line as follow; 1 and 5 mg/L Aaptos suberitoides fraction inhibit 3-4 % cancer cell migration in 24 hours, and 10-11% inhibition in 48 hours, respectively. However, 10 mg/L fraction concentration only inhibited 7-14% of the migration effect.Conclusion: Aaptos suberitoides fraction suggests insignificant migration inhibition in colorectal cancer cells and only inhibits less than 15 % HCT-116 cell line.

scholarly journals Cytotoxic Activity of Stem Bark Extracts from Canarium odontophyllum Miq (Dabai) against Human Colorectal Carcinoma HCT 116 Cell Line

2014 ◽  
Vol 05 (26) ◽  
pp. 3925-3933 ◽  
Author(s):  
Dayang Fredalina Basri ◽  
Muhammad Arifuddin Al Rasyid Mohd ◽  
Chan Kok Meng ◽  
Elda Surhaida Latif ◽  
Fahrul Zaman Huyop
Keyword(s):  
Colorectal Carcinoma ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Stem Bark ◽  
Hct 116 ◽  
Human Colorectal Carcinoma ◽  
Canarium Odontophyllum

scholarly journals Anti-cancer potential of rhenium carbonyl complexes containing pyrrole ligands on colorectal cancer cell line HCT 116

2021 ◽  
Author(s):  
Veeranna Yempally ◽  
Queenie Fernandez ◽  
Lobna Safwan Al_Zaidan ◽  
Varghese Inchakalody ◽  
Maysaloun Merhi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Carbonyl Complexes ◽  
Anti Cancer ◽  
Rhenium Carbonyl ◽  
Hct 116

The Identification of a Novel Unsymmetrical Azine as an Apoptosis Inducer in Colorectal Cancer

2020 ◽  
Vol 20 ◽  
Author(s):  
Fahad M. Almutairi ◽  
Ayat G. Ali ◽  
Abdou O. Abdelhamid ◽  
Adel I. Alalawy ◽  
Mai K. Bishr ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colorectal Carcinoma ◽  
Cell Line ◽  
Binding Affinity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Carcinoma Cell ◽  
Apoptosis Induction ◽  
Colorectal Carcinoma Cell Line ◽  
Hct 116

Background: Defects in the physiological mechanisms of apoptosis are one of the pivotal factors implicated in carcinogenesis. Thus, the development of novel compounds that target various apoptotic pathways has provided promising anticancer therapeutic opportunities. Objective: This study explores the cytotoxic effects of a novel unsymmetrical azine against specific cancer cell lines and investigates the mechanism of cytotoxicity. Methods: Molecular modeling was used to test the binding affinity of four new unsymmetrical azines to a model of an apoptosis inhibitor protein (XIAP). The compound with the highest binding affinity, C4, was further tested on different cell lines. Real-time polymerase chain reaction (PCR) and transmission electron microscope (TEM) were used to study apoptosis induction biochemically and morphologically. Results: In comparison to cisplatin as a control, the compound C4 exhibited notable cytotoxicity against all tested cancer cell lines, especially the human colorectal carcinoma cell line (HCT-116). Furthermore, C4-treated cells demonstrated marked overexpression of the pro-apoptotic proteins Bax and caspase-3 as well as the tumor suppressor p53. On the other hand, the expression of the anti-apoptotic protein Bcl-2 was inhibited. On TEM examination, C4-treated HCT-116 cells showed classical structural signs of apoptosis. Conclusion: This study identifies a novel azine (C4) which induces remarkable cytotoxicity against colorectal carcinoma cell line, mediated through apoptosis induction. These novel insights suggest C4 as a promising therapeutic agent in colorectal cancer.

Silencing the wild-type and mutant K-ras increases the resistance to 5-flurouracil in HCT-116 as a colorectal cancer cell line

2015 ◽  
Vol 26 (2) ◽  
pp. 187-196 ◽  
Author(s):  
Ladan Teimoori-Toolabi ◽  
Saba Hashemi ◽  
Kayhan Azadmanesh ◽  
Farnaz Eghbalpour ◽  
Farnaz Safavifar ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Wild Type ◽  
Hct 116
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