scholarly journals The application of polymerase chain reaction for characterising strains of Pseudomonas syringae isolated from New Zealand rivers

2009 ◽  
Vol 62 ◽  
pp. 256-261 ◽  
Author(s):  
J.L. Vanneste ◽  
D.A. Cornish ◽  
J. Yu ◽  
C.E. Morris
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Chain Reaction ◽  
Plant Pathogenic Bacteria ◽  
Complex Group ◽  
Rivers And Lakes ◽  
Polymerase Chain ◽  
Waikato River

Pseudomonas syringae is a complex group of bacteria which comprises nine different genomospecies and over 50 pathovars Strains of P syringae have been isolated from some rivers and lakes in New Zealand To determine whether these waterways act as reservoirs of plant pathogenic bacteria 15 strains of P syringae isolated from the Waikato River and Whakapapanui stream have been further characterised using several polymerase chain reaction (PCR) protocols Five of those 15 strains belong to genomospecies 1 which comprises P syringae pv syringae but none belongs to genomospecies 2 The protocol for detection of P syringae pv papulans was modified and is now specific for this pathovar The identity of a strain isolated from the Waikato River as being P syringae pv atrofaciens has yet to be confirmed None of the 15 strains studied belongs to the pathovars papulans actinidiae tagetis helianthii or theae

scholarly journals Detection of Pseudomonas syringae pv actinidiae in kiwifruit pollen samples

2011 ◽  
Vol 64 ◽  
pp. 246-251 ◽  
Author(s):  
J.L. Vanneste ◽  
D. Giovanardi ◽  
J. Yu ◽  
D.A. Cornish ◽  
C. Kay ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Pseudomonas Syringae ◽  
Bacterial Canker ◽  
Bacterial Isolation ◽  
Chain Reaction ◽  
Male And Female ◽  
First Report ◽  
Vacuum Device ◽  
Polymerase Chain

Presence of Pseudomonas syringae pv actinidiae (Psa) the causal agent of bacterial canker of kiwifruit in pollen samples collected from infected and non infected orchards in Italy and in New Zealand was determined by polymerase chain reaction (PCR) and by direct bacterial isolation Psa was isolated only from pollen samples collected in Italy including pollen collected from two uninfected orchards which the following year showed signs of infection Psa was also detected in pollen collected from male and female vines in an Italian infected orchard Pollen samples from Italy but not from New Zealand were collected with a vacuum device Psa could not be isolated from any of the 25 New Zealand pollen samples analysed This is the first report of Psa being associated with pollen There is currently no evidence that artificial pollination leads to increased infection or that pollen has been responsible for the introduction of Psa in a previously Psafree area

scholarly journals Detection of Pseudomonas syringae pv papulans in apple budwood

2006 ◽  
Vol 59 ◽  
pp. 146-149 ◽  
Author(s):  
J.L. Vanneste ◽  
J. Yu
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
North America ◽  
Pseudomonas Syringae ◽  
Apple Fruit ◽  
Eastern North America ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Important Disease

Pseudomonas syringae pv papulans (Psp) causes blister spot on apples an economically important disease of the cultivar Mutsu in eastern North America Neither the pathogen nor the disease has been recorded in New Zealand or Australia Since Psp can be transmitted via budwood a protocol to specifically detect Psp in apple buds has been developed It is based on the amplification by polymerase chain reaction (PCR) of part of the hrpL gene Using this protocol presence of Psp could be routinely detected in apple buds spiked with 100 cells of the pathogen This protocol was used to analyse budwoods from Hawkes Bay and Waikato and apple fruit from Waikato Hawkes Bay and Central Otago All samples were negative which is consistent with the pathogen never having been recorded in this country

scholarly journals Identification, Virulence, and Distribution of Two Biovars of Pseudomonas syringae pv. actinidiae in New Zealand

Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 708-719 ◽  
Author(s):  
J. L. Vanneste ◽  
J. Yu ◽  
D. A. Cornish ◽  
D. J. Tanner ◽  
R. Windner ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Disease Progression ◽  
Pseudomonas Syringae ◽  
Electrophoretic Pattern ◽  
Bacterial Canker ◽  
Chain Reaction ◽  
Leaf Spots ◽  
Polymerase Chain ◽  
First Time

Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit, was detected for the first time in New Zealand in November 2010. Only in Bay of Plenty, one of the four regions where this pathogen had been detected, did symptoms evolve beyond leaf spots, resulting in cane die-back, wilting of canes, and canker, sometimes leading to death of the vine. Molecular analysis (cts haplotype and BOX-polymerase chain reaction [PCR] electrophoretic pattern) of strains isolated from different regions of New Zealand revealed that two biovars could be distinguished. They have been called biovar 3 and biovar 4 to differentiate them from strains from Japan (biovar 1) or Korea (biovar 2), which have a different cts haplotype or a different BOX-PCR pattern. Biovars 3 and 4 displayed different degrees of virulence, as measured by their ability to cause leaf spots on young, potted kiwifruit plants. Biovar 3, which has also been present in Italy since 2008 and in France, was found in the Bay of Plenty, where cane diebacks were observed. In contrast, no symptoms other than leaf spots have been observed in orchards where strains of biovar 4 have been isolated. We report the distribution and the disease progression of biovars 3 and 4 in New Zealand.

THE DETECTION OF THE GENUS LISTERIA PATHOGENIC BACTERIA BY REAL-TIME POLYMERASE CHAIN REACTION

2020 ◽  
pp. 117-125
Author(s):  
Elena V. Sokolova ◽  
Ekaterina K. Psareva ◽  
Irina Yu. Egorova ◽  
Pavel A. Zhurilov ◽  
Evgeny A. Potemkin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Pathogenic Bacteria ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Simplified polymerase chain reaction (PCR)-based sexing assists conservation of an endangered owl, the Norfolk Island Boobook Ninox novaeseelandiae undulata

1997 ◽  
Vol 7 (3) ◽  
pp. 283-286 ◽  
Author(s):  
Michael Double ◽  
Penny Olsen
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Second Generation ◽  
Molecular Method ◽  
Chain Reaction ◽  
Norfolk Island ◽  
In The Wild ◽  
Polymerase Chain ◽  
Recovery Effort ◽  
Main Factor

In 1986 a single Norfolk Island Boobook Owl Ninox novaeseelandiae undulata remained. As part of a re-establishment programme, two male New Zealand Moreporks N. n. novaeseelandiae were introduced, one of which survived to pair with the female in the wild and breed successfully. By 1995 the population numbered 12 or 13 individuals of which seven were second generation (F2). However, there were only two breeding pairs. As the 11 hybrids could not be sexed using morphometrics we developed a molecular method based on a recently described avian polymerase chain reaction (PCR)-based sexing technique. The population was found to contain six females and five males. A scarcity of mature males was established as the main factor slowing the recovery effort.

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