scholarly journals Simplified polymerase chain reaction (PCR)-based sexing assists conservation of an endangered owl, the Norfolk Island Boobook Ninox novaeseelandiae undulata

1997 ◽  
Vol 7 (3) ◽  
pp. 283-286 ◽  
Author(s):  
Michael Double ◽  
Penny Olsen
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Second Generation ◽  
Molecular Method ◽  
Chain Reaction ◽  
Norfolk Island ◽  
In The Wild ◽  
Polymerase Chain ◽  
Recovery Effort ◽  
Main Factor

In 1986 a single Norfolk Island Boobook Owl Ninox novaeseelandiae undulata remained. As part of a re-establishment programme, two male New Zealand Moreporks N. n. novaeseelandiae were introduced, one of which survived to pair with the female in the wild and breed successfully. By 1995 the population numbered 12 or 13 individuals of which seven were second generation (F2). However, there were only two breeding pairs. As the 11 hybrids could not be sexed using morphometrics we developed a molecular method based on a recently described avian polymerase chain reaction (PCR)-based sexing technique. The population was found to contain six females and five males. A scarcity of mature males was established as the main factor slowing the recovery effort.

scholarly journals The search for a vector of strawberry lethal yellows (SLY) in New Zealand

2002 ◽  
Vol 55 ◽  
pp. 385-389 ◽  
Author(s):  
J.G. Charles ◽  
D.J. Allan ◽  
M.T. Andersen ◽  
G. Langford ◽  
D. Mossop
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Second Generation ◽  
Insect Vectors ◽  
Chain Reaction ◽  
Sticky Traps ◽  
Plant Hosts ◽  
Polymerase Chain ◽  
First And Second Generation ◽  
Yellow Sticky Traps

Strawberry lethal yellows (SLY) is a phytoplasma disease that affects strawberry plants in New Zealand and Australia Although it has been established that this phytoplasma (Candidatus Phytoplasma australiense) is responsible for causing diseases in a number of other plant hosts an insect responsible for its spread still remains to be determined To identify potential insect vectors yellow sticky traps were established at two properties in the Bay of Plenty from planting (early October 2001) until harvest (May 2002) Leafhoppers caught in the traps until March 2002 and by sweepnetting the surrounding habitats in February were identified and tested for the presence of phytoplasma using Polymerase Chain Reaction (PCR) Arawa variegata and Recilia hospes (both Cicadellidae) were the commonest leafhoppers trapped Phytoplasma was detected in first and second generation A variegata in October and February respectively and in second generation R hospes in February The implications of this work for future SLY studies is discussed

scholarly journals Molecular Typing of Human Brucella melitensis Isolated from Patients in Erbil, Iraq

2019 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Bushra K. Amin ◽  
Khulod I. Hassan
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Typing ◽  
Brucella Melitensis ◽  
Control Strategies ◽  
Human Brucellosis ◽  
Kurdistan Region ◽  
Specific Primer ◽  
Molecular Method ◽  
Chain Reaction ◽  
Polymerase Chain

Brucellosis is a reemerging infectious zoonotic disease of worldwide importance. In the Kurdistan Region of Iraq, it is a widely spread disease and remains a challenging health problem. This disease is mainly caused by Brucella melitensis, in human. For confirmation of these isolates, a study was performed, by isolation and molecular typing of Brucella Spp. from human patients in Rizgari Hospital at Erbil city (Iraq), between March 2014 and November 2016. One hundred sixty seven samples of blood collected from patients suspected for brucellosis, one hundred twenty one samples from these were recorded as genus of Brucella, using biochemical test and confirmed by applying polymerase chain reaction (PCR), using genus specific primer for omp31 gene which was specific for B. melitensis. These results support using molecular method that based on PCR as diagnostic test for the control of brucellosis in Erbil. Further studies are needed from different geographical areas of the country with different level of endemicity to plan and execute control strategies against human brucellosis.

scholarly journals Daignosis of Staphylococcus aureus mastitis in bovine in Al-Najaf province by using Polymerase chain reaction (PCR)

2013 ◽  
Vol 12 (2) ◽  
pp. 81
Author(s):  
N. A. Al- Anbagi
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Subclinical Mastitis ◽  
Molecular Method ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Significant Incidence ◽  
Left Posterior ◽  
Polymerase Chain

This study was conducted to collect 388 milk samples from cows at different villages and townships in Al-Najaf province to examine about Staphylococcus aureus mastitis .CMT was used for subclinical mastitis screening ,212(54.6%) milk samples were mastitic .The molecular method (PCR assay) was used to detected the presence (glpF) gene in classically diagnosed S.aureus, which appeared that 38(92.6%) S.aureus mastitis as 13(32.5%) clinical and 25(14.5%) subclinical mastitis .There was high significant incidence of Staphylococcus aureus mastitis in left posterior udder quarter rather than others quarters

scholarly journals The application of polymerase chain reaction for characterising strains of Pseudomonas syringae isolated from New Zealand rivers

2009 ◽  
Vol 62 ◽  
pp. 256-261 ◽  
Author(s):  
J.L. Vanneste ◽  
D.A. Cornish ◽  
J. Yu ◽  
C.E. Morris
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Chain Reaction ◽  
Plant Pathogenic Bacteria ◽  
Complex Group ◽  
Rivers And Lakes ◽  
Polymerase Chain ◽  
Waikato River

Pseudomonas syringae is a complex group of bacteria which comprises nine different genomospecies and over 50 pathovars Strains of P syringae have been isolated from some rivers and lakes in New Zealand To determine whether these waterways act as reservoirs of plant pathogenic bacteria 15 strains of P syringae isolated from the Waikato River and Whakapapanui stream have been further characterised using several polymerase chain reaction (PCR) protocols Five of those 15 strains belong to genomospecies 1 which comprises P syringae pv syringae but none belongs to genomospecies 2 The protocol for detection of P syringae pv papulans was modified and is now specific for this pathovar The identity of a strain isolated from the Waikato River as being P syringae pv atrofaciens has yet to be confirmed None of the 15 strains studied belongs to the pathovars papulans actinidiae tagetis helianthii or theae

scholarly journals Detection of Pseudomonas syringae pv actinidiae in kiwifruit pollen samples

2011 ◽  
Vol 64 ◽  
pp. 246-251 ◽  
Author(s):  
J.L. Vanneste ◽  
D. Giovanardi ◽  
J. Yu ◽  
D.A. Cornish ◽  
C. Kay ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Pseudomonas Syringae ◽  
Bacterial Canker ◽  
Bacterial Isolation ◽  
Chain Reaction ◽  
Male And Female ◽  
First Report ◽  
Vacuum Device ◽  
Polymerase Chain

Presence of Pseudomonas syringae pv actinidiae (Psa) the causal agent of bacterial canker of kiwifruit in pollen samples collected from infected and non infected orchards in Italy and in New Zealand was determined by polymerase chain reaction (PCR) and by direct bacterial isolation Psa was isolated only from pollen samples collected in Italy including pollen collected from two uninfected orchards which the following year showed signs of infection Psa was also detected in pollen collected from male and female vines in an Italian infected orchard Pollen samples from Italy but not from New Zealand were collected with a vacuum device Psa could not be isolated from any of the 25 New Zealand pollen samples analysed This is the first report of Psa being associated with pollen There is currently no evidence that artificial pollination leads to increased infection or that pollen has been responsible for the introduction of Psa in a previously Psafree area

Detection of Listeria monocytogenes in Salmon Using the Probelia Polymerase Chain Reaction System

2003 ◽  
Vol 66 (3) ◽  
pp. 436-440 ◽  
Author(s):  
JASON WAN ◽  
KERRYN KING ◽  
SANTINA FORSYTH ◽  
M. JOHN COVENTRY
Keyword(s):  
Polymerase Chain Reaction ◽  
New Zealand ◽  
Listeria Monocytogenes ◽  
Reaction System ◽  
Food Microbiology ◽  
Detection Sensitivity ◽  
Broth Culture ◽  
Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain

A validation was conducted on the performance of a commercially available polymerase chain reaction (PCR) kit (Probelia) in comparison with International Organization for Standardization (ISO) method 11290-1 (adopted as an Australian New Zealand Standard Method, AS/NZS 1766.2.16.1:1998) for the detection of Listeria monocytogenes in salmon samples. The validation was conducted following the guidelines of an Australian New Zealand Standard (Guide to Determining the Equivalence of Food Microbiology Test Methods, Part 1, Qualitative Tests, AS/NZS 4659.1:1999), which adopts an approach similar to that recommended by the Association of Analytical Communities Microbiology Method Validation Program for Performance Tested and Peer Verified Methods. The validation study involved the use of five cultures of L. monocytogenes, each challenged at a single level of inoculation into five different types of salmon samples. A total of 60 salmon samples (30 unchallenged and 30 challenged) were tested using both the PCR method and the ISO method. Results from this study indicated that the Probelia PCR method is equivalent to the ISO method. In addition, the detection sensitivity of the Probelia PCR system was determined as approximately 0.5 CFU per PCR assay (equivalent to 20 CFU/ml broth culture) for a pure culture of L. monocytogenes. The Probelia PCR method offers the advantage of detecting L. monocytogenes to genetic specificity within 48 to 50 h, whereas the ISO method requires 5 days for negative results with additional days for confirmed positive results by the use of other biochemical and cultural tests.

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