scholarly journals Monitoring of bacterial agents isolated from small cattle on the territory of certain regions of the Russian Federation

2021 ◽  
Author(s):  
P.N. Shastin ◽  
A.V. Kapustin ◽  
E.A. Yakimova ◽  
E.V. Ivanov ◽  
A.I. Laishevtsev
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Clostridium Perfringens ◽  
Russian Federation ◽  
Pasteurella Multocida ◽  
Mannheimia Haemolytica ◽  
Sheep Breeding ◽  
Trueperella Pyogenes ◽  
The Russian Federation ◽  
Bibersteinia Trehalosi

The paper presents the results of bacterial screening of goat and sheep breeding enterprises in certain regions of Russia (Tver, Moscow, Smolensk regions, as well as the Republic of Mari-El and Tatarstan), conducted in the period from 2018 to 2021. In the course of this work, 556 samples of sectional material (heart, lungs, gastrointestinal tract, spleen, kidneys, liver, lymph nodes, breast, flushes from the genitourinary system, as well as exudate from purulent lesions) were subjected to a comprehensive bacteriological study. As a result of the conducted studies, 1223 isolates belonging to 25 families (111 bacterial species) were isolated and identified (by the method of time-of-flight mass spectrometry MALDI-ToF). According to the data obtained, the incidence of Escherichia coli isolation was 10.95%, Trueperella pyogenes – 5.47%, Staphylococcus aureus – 5.31%, Proteus mirabilis – 4.08%, Mannheimia haemolytica – 4.00%, Enterococcus faecalis – 3.76%, Enterobacter cloacea and Staphylococcus haemolyticus – 3.59%, Streptococcus dysgalactiae – 3.51%, Pasteurella multocida – 3.27%, Acinetobacter lwoffii – 2.78%, Staphylococcus cohnii – 2.61%, Bibersteinia trehalosi – 2.29%, Pseudomonas aeruginosa – 2.12%, Bacillus cereus and Micrococcus luteus – 1.96%, Corynebacterium pseudotuberculosis and Staphylococcus equorum – 1.88%, Aerococcus viridans – 1.80%, Corynebacterium xerosis – 1.72%, Clostridium perfringens, Streptococcus mitis and Streptococcus pyogenes – 1.39%, Staphylococcus chromogenes and Streptococcus entericus – 1.14%, respectively. The incidence of isolation of other types of microorganisms was below 1%. The data obtained indicate the circulation of a wide range of bacteria in goat and sheep breeding enterprises of the Russian Federation, some of which should be positioned as pathogenic flora (for example, Pasteurella multocida, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, Clostridium perfringens, etc.), some as conditionally pathogenic (Trueperella pyogenes, Staphylococcus aureus, Bibersteinia trehalosi, Mannheimia haemolytica, Pseudomonas aeruginosa, Moraxella bovis, Moraxella bovoculi, etc.), as well as the normal flora of the animal body. It is worth focusing on these data when conducting a survey of livestock enterprises in order to establish an objective epizootic situation, including taking into account the possibility of circulating pathogens of factor diseases.

Pasteurellaceae prevalence in livestock farms in different regions of Russian Federation

2021 ◽  
Vol 1 (5) ◽  
pp. 18-25
Author(s):  
Yu. I. Pobolelova ◽  
◽  
S. P. Yatsentyuk ◽  
A. D. Kozlova ◽  
D. A. Rudnyaev ◽  
...  
Keyword(s):  
Russian Federation ◽  
Pasteurella Multocida ◽  
Type A ◽  
Mannheimia Haemolytica ◽  
Livestock Farms ◽  
Bibersteinia Trehalosi ◽  
Regions Of Russia

The article presents the results of a PCR study of the prevalence of Pasteurella multocida, Mannheimia haemolytica, Bibersteinia trehalosi and Histophillus somni in livestock farms in 10 regions of Russia. In animals without signs of disease, P. multocida was the most common (25%), the rarest was Bibersteinia trehalosi (<1%). Most often, Pasteurellaceae bacteria were found in Tatarstan and Udmurtia; P. multocida type A was found in 96% and 89% of samples, respectively.

Epidemiological characteristics of staphylococcal meningitis in Russian Federation

2021 ◽  
pp. 51-54
Author(s):  
G. V. Beloshitskiy ◽  
I. S. Koroleva ◽  
M. A. Koroleva
Keyword(s):  
Staphylococcus Aureus ◽  
Mortality Rate ◽  
Bacterial Meningitis ◽  
Russian Federation ◽  
Common Cause ◽  
Probability Of Death ◽  
The Russian Federation ◽  
Epidemiological Characteristics

Staphylococcal meningitis (SM) ranks fourth in the etiological structure of purulent bacterial meningitis in the Russian Federation. The most common cause of staphylococcal meningitis is Staphylococcus aureus, which accounts for 68 % of all cases. This meningitis is characterized by a higher mortality rate (30 %) than staphylococcal meningitis of other types of Staphylococcus (13 %) and mainly affects people over 25 years of age. With increasing age, the probability of death increases, and among people over 65 years of age, the mortality rate reaches 44 %.

scholarly journals Development of a multiplex PCR for simultaneous detection of Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes

2017 ◽  
Vol 65 (3) ◽  
pp. 327-339 ◽  
Author(s):  
Wenlong Zhang ◽  
Xiaodan Liu ◽  
Mengcheng Liu ◽  
Bo Ma ◽  
Li Xu ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Genomic Dna ◽  
Pasteurella Multocida ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Laboratory Diagnosis ◽  
Mannheimia Haemolytica ◽  
Bacterial Strains ◽  
Trueperella Pyogenes ◽  
Enrichment Technology

Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.

scholarly journals ЧЕМ ЗАМЕНИТЬ АНТИБИОТИКИ В ПТИЦЕВОДСТВЕ? Йылдырым Е.А., Ильина Л.А., Тюрина Д.Г., Дубровин А.В., Филиппова В.А., Новикова Н.И., Большаков В.Н., Лаптев Г.Ю.

2020 ◽  
Vol 69 (9) ◽  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Clostridium Perfringens ◽  
Salmonella Enteritidis ◽  
Pasteurella Multocida

Из-за постоянного и несистемного применения антибиотиков в животноводстве и птицеводстве эффективность их воздействия на макроорганизм заметно падает, так как патогенные бактерии достаточно быстро вырабатывают антибиотикорезистентность - устойчивость к данным лекарственным веществам. Свести к минимуму применение антибиотиков без ущерба для производителя птицепродукции возможно даже на крупных промышленных предприятиях с большой плотностью поголовья птицы. В первую очередь, следует отказываться от антибиотиков, предназначенных для профилактических целей и стимуляции роста. Эту роль можно и нужно доверить безопасным альтернативным вариантам. В настоящее время на рынке появляются натуральные растительные биопрепараты на основе эфирных масел, микроорганизмов и их полезных метаболитов, не уступающие по эффективности антибиотикам, но исключающие негативные последствия от их применения. Так, фитобиотик Интебио стимулирует активный иммунный ответ у бройлеров, что сопровождается увеличением продуктивности. Пробиотик Профорт на основе штаммов полезных бактерий обладает высоким уровнем антимикробной активности в отношении кишечных патогенов Salmonella enteritidis, Pasteurella multocida, Clostridium perfringens, Staphylococcus aureus, Escherichia coli и др. Метапробиотик Пробиоцид-Ультра на основе пробиотических штаммов бактерий, дополнительно обогащенный полезными бактериальными метаболитами, работает подобно антибиотикам, как лечебным, подавляя патогенную микрофлору, так и кормовым, стимулируя увеличение продуктивности.

scholarly journals Nem todo abscesso em pequenos ruminantes é causado por Corynebacterium pseudotuberculosis

2018 ◽  
Vol 38 (10) ◽  
pp. 1902-1908
Author(s):  
Reanne M.M. Silva ◽  
Robson B. Cerqueira ◽  
Vinicius P. Vieira ◽  
Jorge R.L. Ribas ◽  
Karla A. Nascimento ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Corynebacterium Pseudotuberculosis ◽  
Trueperella Pyogenes

RESUMO: O presente estudo objetivou determinar a prevalência e distribuição de lesões abscedativas, identificação do agente etiológico e avaliação das lesões histológicas em caprinos e ovinos abatidos em um matadouro-frigorífico com Serviço de Inspeção Federal do estado da Bahia. Foram coletadas 153 amostras de vísceras e linfonodos com abscessos de 1.148 animais abatidos. A maior prevalência na espécie ovina foi em macho, com faixa etária de 12 meses, sendo os principais órgãos acometidos fígado (21,2%) e linfonodo pré-escapular (20,3%). Na espécie caprina, a prevalência maior foi em macho, com faixa etária de 30 meses, sendo os linfonodos retro faríngeo (25%) e pré-escapular os mais acometidos (25%). Isolou-se os seguintes micro-organismos das amostras: Corynebacterium pseudotuberculosis em 33,33%, Escherichia coli (19,61%), Proteus mirabilis (9,80%), Pseudomonas aeruginosa (7,19%), Trueperella pyogenes (5,22%), Streptococcusspp. (5,22%) e Staphylococcus aureus (4,57%). As lesões macroscópicas e histológicas dos abscessos coletados não apresentaram diferenças entre micro-organismos isolados.

scholarly journals Analysis and Study on the Degradation of Sarfloxacin Hydrochloride by BiVO4 Synthetic Material

2021 ◽  
Vol 898 (1) ◽  
pp. 012026
Author(s):  
Yue Zhang ◽  
Wenbin Liu ◽  
Liansheng Yu ◽  
Kuilin Lv ◽  
Yuguang Lv
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Graphene Oxide ◽  
Degradation Product ◽  
Respiratory Diseases ◽  
Degradation Rate ◽  
Pasteurella Multocida ◽  
Synthetic Material ◽  
Chronic Respiratory Diseases

Abstract Difloxacin hydrochloride is commonly used in livestock and poultry chronic respiratory diseases, tracheitis, enteritis, pneumonia, avian cholera, Streptococcosis, typhoid and other diseases caused by sensitive bacteria such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteus and Pasteurella multocida, especially for Chicken Colibacillosis, piglet red, yellow and white dysentery. The prepared BiVO4 synthetic material was used for photodegradation of the target degradation product of salfloxacin hydrochloride. The degradation rate of the sample to salfloxacin hydrochloride was 24.1%; The degradation rate of the composite sample (Polypyrrole/graphene oxide/bismuth vanadate) reached 75.57%. The degradation rate was 5 times that of BiVO4.

scholarly journals Isolation et antibiogramme de la flore bactérienne aérobie des cavités nasales de chèvres Naines d’Afrique de l’Ouest apparemment saines

2009 ◽  
Vol 62 (1) ◽  
pp. 17
Author(s):  
B. O. Emikpe ◽  
O. G. Oyero ◽  
S. O. Akpavie
Keyword(s):  
Staphylococcus Aureus ◽  
Pasteurella Multocida ◽  
Mannheimia Haemolytica ◽  
Professionnels De Santé ◽  
Flore Bactérienne

Les chèvres jouent un rôle important dans l’économie de l’élevage parce qu’elles sont une source de protéines et de revenus essentiels pour les pauvres paysans, et parce qu’elles s’adaptent à des conditions environnementales difficiles. Les études sur la flore bactérienne de l’appareil respiratoire des chèvres ont principalement porté sur les lésions pneumotiques ; peu d’études ont été réalisées sur les cavités nasales apparemment normales et peu d’antibiogrammes des organismes isolés ont été effectués. La présente étude a été menée sur 60 chèvres Naines d’Afrique de l’Ouest apparemment saines. Un écouvillon nasal a été effectué sur chaque chèvre et analysé avec les méthodes standard. La méthode de diffusion en gélose a été utilisée pour le test de sensibilité des antibiotiques. Trois cent vingt-huit isolats on été obtenus. Streptococcus spp. a été la bactérie rencontrée le plus fréquemment, suivie d’Escherichia coli et de Staphylococcus aureus. D’autres espèces ont été isolées à des taux relativement plus faibles. La présence de Mannheimia haemolytica et de Pasteurella multocida dans les cavités nasales de chèvres apparemment saines indiquerait leur rôle potentiel dans la plupart des pathologies respiratoires courantes des petits ruminants. La majorité des bactéries ont été sensibles à la streptomycine, aux quinolones (perfloxacine, ciprofloxacine et ofloxacine) et à la gentamicine, alors qu’elles ont été résistantes à la tétracycline, à l’augmentin et à l’érythromycine. Cette étude a montré la relation entre le mauvais usage ou l’abus d’antibiotiques, et la résistance aux médicaments. Il est ainsi essentiel d’informer les professionnels de santé et les chercheurs sur les antibiotiques adaptés à utiliser lors d’infections respiratoires et au cours de programmes de lutte.

scholarly journals Pathology and pathogenesis of bovine skin and meibomian gland demodicosis

2015 ◽  
Vol 67 (2) ◽  
pp. 77 ◽  
Author(s):  
M. T. Abu-Samra ◽  
Y. A. Shuaib
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Meibomian Gland ◽  
Post Mortem ◽  
Proteus Vulgaris ◽  
Trueperella Pyogenes ◽  
Moraxella Bovis

Une enquête nationale menée au Soudan sur la démodécie bovine a concerné 48  000 animaux examinés soit au cours de campagnes de vaccination (44 800), soit lors d’inspections ante et post mortem dans des abattoirs (3 200). Sur l’ensemble des animaux enquêtés, 44  908 étaient des adultes (2-8 ans) dont 34,6 p. 100 étaient infectés, et 3 092 étaient des veaux (< 2 ans) dont 34,6 p. 100 étaient infectés. Trois cents bovins affectés par des lésions cutanées graves, dont 218 présentaient également des lésions de la glande de Meibomius, ont été sélectionnés. Les tableaux cliniques de la démodécie cutanée et de la démodécie de la glande de Meibomius ont été décrits. Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, Sta. epidermidis, Streptococcus pyogenes (groupe A) et Trueperella pyogenes ont été isolés dans les lésions cutanées, et Moraxella bovis et Sta. aureus l’ont été dans les lésions de la glande de Meibomius. Ces bactéries produisaient des toxines et des enzymes qui aggravaient les lésions causées par les acariens Demodex bovis et D. ghanensis, respectivement dans la peau et dans les glandes de Meibomius. Aucun de ces acariens n’a été retrouvé dans les tissus internes ni dans les organes, indiquant qu’il n’y a pas eu de phase endoparasitaire. Les modifications histopathologiques observées étaient liées à l’immunité à médiation cellulaire. L’écoulement du contenu des colonies de Demodex dans les couches sous-épidermique et dermique, et dans les tissus environnant les glandes de Meibomius a entraîné des modifications histopathologiques sévères, caractérisées par le renouvellement continu des réactions granulomateuses avec afflux de macrophages et de lymphocytes. La pathogenèse de la maladie a été décrite du stade d’invasion initiale des follicules pileux et des tubes collecteurs des glandes de Meibomius par les acariens et les bactéries associées, au stade de régression des lésions. Il a été conclu que la nature de l’association entre les acariens Demodex et les bactéries dans les lésions démodéciques était synergique et que les deux agents pathogènes avaient la même importance.

scholarly journals P151 Diversity of Staphylococcus aureus clones among cystic fibrosis patients in the Russian Federation

2019 ◽  
Vol 18 ◽  
pp. S100
Author(s):  
L. Avetisyan ◽  
M. Chernukha ◽  
O. Medvedeva ◽  
I. Shaginyan ◽  
A. Prilipov ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Russian Federation ◽  
The Russian Federation

scholarly journals Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

2012 ◽  
Vol 78 (18) ◽  
pp. 6683-6688 ◽  
Author(s):  
Jegarubee Bavananthasivam ◽  
Rohana P. Dassanayake ◽  
Abirami Kugadas ◽  
Sudarvili Shanthalingam ◽  
Douglas R. Call ◽  
...  
Keyword(s):  
Pore Size ◽  
Pasteurella Multocida ◽  
Ovis Canadensis ◽  
Mannheimia Haemolytica ◽  
Experimental Conditions ◽  
Content Type ◽  
Inhibition Of Growth ◽  
Dependent Inhibition ◽  
Bibersteinia Trehalosi

ABSTRACTMannheimia haemolytica,Pasteurella multocida, andBibersteinia trehalosihave been identified in the lungs of pneumonic bighorn sheep (BHS;Ovis canadensis). Of these pathogens,M. haemolyticahas been shown to consistently cause fatal pneumonia in BHS under experimental conditions. However,M. haemolyticahas been isolated by culture less frequently than the other bacteria. We hypothesized that the growth ofM. haemolyticais inhibited by other bacteria in the lungs of BHS. The objective of this study was to determine whetherP. multocidainhibits the growth ofM. haemolytica. Although in monoculture both bacteria exhibited similar growth characteristics, in coculture withP. multocidathere was a clear inhibition of growth ofM. haemolytica. The inhibition was detected at mid-log phase and continued through the stationary phase. When cultured in the same medium, the growth ofM. haemolyticawas inhibited when both bacteria were separated by a membrane that allowed contact (pore size, 8.0 μm) but not when they were separated by a membrane that limited contact (pore size, 0.4 μm). Lytic bacteriophages or bactericidal compounds could not be detected in the culture supernatant fluid from monocultures ofP. multocidaor fromP. multocida-M. haemolyticacocultures. These results indicate thatP. multocidainhibits the growth ofM. haemolyticaby a contact- or proximity-dependent mechanism. If the inhibition of growth ofM. haemolyticabyP. multocidaoccursin vivoas well, it could explain the inconsistent isolation ofM. haemolyticafrom the lungs of pneumonic BHS.

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