Features of CD-­­phenotype of peripheral blood lymphocytes in adult patients with atopic dermatitis during the exacerbation

2021 ◽  
pp. 16-22
Author(s):  
O.D. Aleksandruk
Keyword(s):  
Atopic Dermatitis ◽  
Peripheral Blood ◽  
Clinical Manifestations ◽  
Peripheral Blood Lymphocytes ◽  
Peripheral Lymphocyte ◽  
Adult Patients ◽  
Total Serum ◽  
Control Group ◽  
Blood Lymphocytes ◽  
Number Of Cells

Objective — to study the dynamics of peripheral blood lymphocytic populations in adult patients with atopic dermatitis (AD) with the onset of the disease in childhood, depending on the level of IgE secretion and the method of treatment. Materials and methods. We examined 67 adult patients with AD, which were divided into 4 groups depending on the level of total serum IgE and the basic treatment or treatment in combination with Glycine and Ketotifen. The severity of AD was determined by the SCORAD index. The content of peripheral blood lymphocytes according to the phenotype CD3+, CD4+, CD8+, CD19+, CD65+, HLADR+ and CD95+ was assessed during hospitalization of patients, at the end of the inpatient stage of treatment and after 1 month of outpatient follow-up. The obtained data were compared with the indices of the control group and between the groups of examined patients with AD in the dynamics of their treatment and observation. The results were processed statistically using the methods of parametric and nonparametric statistics. Results and discussion. The indices of the number of cells of peripheral lymphocytic populations of different CD pheno­type in the groups in the dynamics of observation were determined, their relationship with the severity of the course of AD was established, and differences were found depending on the pathogenetic variant of AD. Against the background of an exacerbation of AD, a significant increase in the number of cells in most of the defined populations was revealed, with its gradual decrease as the clinical manifestations of AD subsided. It was established that 1 month after achievement of clinical/subclinical remission, a part of the peripheral blood lymphocytic populations was characterized by higher values compared to the norm. In patients with an IgE-dependent AD variant, aggravation is accompanied by high levels of peripheral lymphocytes with CD3+, CD4+, CD8+, CD19+ and HLA-DR phenotypes, which more often than in the case of an IgE-independent variant of AD, remain above the norm after 1 month of outpatient monitoring. Introduction of glycine and ketotifen to the treatment complex for patients with AD is accompanied by a faster return of peripheral lymphocyte cells to normal values, which is more evident in patients with an IgE-dependent variant of AD. Conclusions. In adult AD patients, the dynamics of the number of peripheral lymphocyte population cells depends on the severity of the disease, its pathogenetic variant and the treatment received by the patient. Against the background of the use of glycine and ketotifen, the normalization of indicators of peripheral lymphocytic populations occurs significantly faster than with only standard basic therapy.

scholarly journals Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising Radiation

2008 ◽  
Vol 59 (4) ◽  
pp. 251-259 ◽  
Author(s):  
Vilena Kašuba ◽  
Ružica Rozgaj ◽  
Anamarija Jazbec
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Direct Detection ◽  
Hospital Staff ◽  
Peripheral Blood Lymphocytes ◽  
Ionising Radiation ◽  
Control Group ◽  
Blood Lymphocytes ◽  
Occupationally Exposed ◽  
Low Levels

Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising RadiationMedical staff is an occupational group exposed to different agents suspected to induce genetic damage. Among them ionising radiation is the most studied. Cytogenetic analysis of human chromosomes in peripheral lymphocytes allows direct detection of mutation in somatic cells. This study investigated the cytogenetic effects of low-level ionising x-radiation in 48-hour peripheral blood lymphocyte cultures sampled from 765 hospital staff occupationally exposed to several agents known or suspected to induce chromosome damage and compared them with 200 control subjects. The exposed subjects were divided in eight (8) groups according to their specialities and job titles. The exposed groups manifested an increase in all types of chromosome aberrations. Acentric fragments were the most frequent chromosome-type aberration. Dicentric chromosomes were statistically significant only in urologists/gynaecologists. Age and smoking significantly influenced the incidence of dicentrics in the exposed groups. The frequency of ring chromosomes was low in all exposed groups (range: 0-2), and none were found in the control group. These findings indicate the importance of periodic medical checkups of hospital staff occupationally exposed to low doses of ionising radiation. The purpose is to create an individual cytogenetic register, where changes could evidence individual risks.

Melatonin a Promising Candidate for DNA Double-Stranded Breaks Reduction in Patients Undergoing Abdomen-Pelvis Computed Tomography Examinations

2020 ◽  
Vol 20 (7) ◽  
pp. 859-864
Author(s):  
Ali Eskandari ◽  
Aziz Mahmoudzadeh ◽  
Alireza Shirazi ◽  
Farid Esmaely ◽  
Carla Carnovale ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Oral Dose ◽  
Single Oral Dose ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Promising Candidate ◽  
Blood Lymphocytes ◽  
Ct Examination

Background and Objective: Cancer incidence is 24% higher in children and young adults exposed to Computed Tomography (CT) scans than those unexposed. Non-repairing of ionizing radiation-induced DNA Double-Strand Breaks (DSBs) can initiate carcinogenesis. In the present study, we aimed to investigate the radioprotective potential of melatonin against DSBs in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations. Methods: This double-blind, placebo-controlled clinical trial was conducted on thirty patients. These patients were divided into two groups; group one (control) patients who have undergone the CT examination received a single oral dose of placebo, while in group two, patients received a single oral dose of 100mg melatonin. In both the groups, blood samples were collected 5-10min before and 30 minutes after the CT examination. The lymphocytes from these samples were isolated and DSBs were analyzed using γH2AX immunofluorescence microscopy. Results: Compared to the control group, the use of melatonin 1h before the CT examination caused a significant reduction in γH2AX-foci, indicating a reduction in DSBs. In addition, no side effect was observed in patients following 100mg melatonin administration. Conclusion: For the first time, this study has shown that melatonin has protective effects against radiationinduced genotoxicity in peripheral blood lymphocytes of patients undergoing abdomen-pelvis CT examinations. Therefore, melatonin can be considered as a promising candidate for reducing DSBs in patients undergoing abdomen-pelvis CT examinations.

Genetic variation and risk of DNA damage in peripheral blood lymphocytes of Iranian formaldehyde-exposed workers

2017 ◽  
Vol 37 (7) ◽  
pp. 690-696
Author(s):  
Rezvan Zendehdel ◽  
Parviz Abdolmaleki ◽  
Fatemeh Javani Jouni ◽  
Mohammad Mazinani
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Control Population ◽  
Control Group ◽  
Special Role ◽  
Effective Parameters ◽  
Blood Lymphocytes ◽  
Exposed Population

Formaldehyde (FA) has given positive results for the genetic damage evaluated in human peripheral blood lymphocytes. Polymorphism plays a special role in the toxicity of chemicals. DNA damage in blood cells was evaluated in workers who were occupationally exposed to FA. This study tested the association of alcohol dehydrogenase III (ADH3) and manganese-superoxide dismutase (Mn-SOD) gene polymorphism, with DNA damage of peripheral blood lymphocytes. Gene polymorphisms were evaluated in 54 workers from melamine dinnerware workshops and 34 workers from dairy production workshops. The control and exposed population were matched based on age, smoking, work history and socioeconomic status. DNA damage was evaluated by alkaline comet assay in the peripheral blood lymphocytes. ADH3 and Mn-SOD genotypes were determined in all workers using polymerase chain reaction–restriction fragment length polymorphism method. DNA damage of lymphocyte cells was significantly higher ( p < 0.001) in the exposed individuals in comparison with the control population. The examinations of the exposed group had revealed significantly higher ( p < 0.01) variant of Val/Val of Mn-SOD compared to control group. In workers with ADH32–2 variant, significantly higher levels of DNA damage ( p < 0.01) were observed than the control population with the same genotype. It could be concluded that enzymes involved in the metabolism of FA and oxidative stress controlling are two important effective parameters for DNA damage of peripheral blood lymphocytes in exposed people. These results are regarded as a pioneer investigation for the management of health risks assessment.

Characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion

1980 ◽  
Vol 47 (3) ◽  
pp. 305-311 ◽  
Author(s):  
Carlos Concha ◽  
Olof Holmberg ◽  
Bror Morein
Keyword(s):  
T Cells ◽  
Salmonella Typhimurium ◽  
Concanavalin A ◽  
Peripheral Blood ◽  
Helix Pomatia ◽  
Peripheral Blood Lymphocytes ◽  
Pokeweed Mitogen ◽  
Dry Period ◽  
Blood Lymphocytes ◽  
Number Of Cells

SUMMARYFrom bovine mammary secretion during the dry period, the total number of cells was between 1·2 and 5·9 Ǻ 106/ml. A mean of 35% of these cells were classified as lymphocytes and ∼ 85% of them could be isolated by the Ficoll-isopac method. Centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. About 47% of the lymphocytes bound Helix pomatia agglutinin, a T-cells marker, while the proportion of Ig-bearing cells was ∼ 28%. The mammary lymphocytes were stimulated by the lectins phytohaemagglutinin, pokeweed mitogen, concanavalin A and by lipopolysaccharide from Salmonella typhimurium. The stimulation indices of mammary lymphocytes were generally lower than those for peripheral blood lymphocytes from the same animals. The background values, i.e. counts/min of lymphocytes incubated without mitogen, were often higher for lymphocytes isolated from mammary secretion than from blood.

scholarly journals Effects of sevoflurane exposure on apoptosis and cell cycle of peripheral blood lymphocytes, and immunologic function

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhimin Ji ◽  
Wanjun Wu ◽  
Fan Zhou ◽  
Junfang Hu ◽  
Qiuping Xu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Operating Room ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Venous Blood ◽  
Peripheral Blood Lymphocytes ◽  
Exposed Group ◽  
Control Group ◽  
Blood Lymphocytes

Abstract Background Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, however, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room. Methods A cohort of 56 medical residents were divided into exposed group (n = 28) and control group (non-exposed group) (n = 28). Gas chromatography was used to measure the concentration of sevoflurane in the medical resident’s breathing zone during surgeries under inhalation anesthesia in the exposure group. The gas collection lasted an hour. Peripheral blood lymphocytes were isolated from venous blood, and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry. Results The average concentration of sevoflurane in the exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. No significant effects were found on the apoptosis rates or cell cycles of peripheral blood lymphocytes in the exposed group relative to the control group (P > 0.05). Similarly, there were no significant differences in the lymphocyte subsets or the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P > 0.05). Conclusions Occupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples. Trial registration number and date of registration ChiCTR2000040772, December 9, 2020 (Retrospective registration).

scholarly journals Assessment of CD11b and CD11/18 integrin expression on leukocytes of dogs with atopic dermatitis

2014 ◽  
Vol 58 (1) ◽  
pp. 99-103
Author(s):  
Iwona Taszkun
Keyword(s):  
Atopic Dermatitis ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Integrin Expression ◽  
Blood Lymphocytes ◽  
Surface Molecules ◽  
Healthy Dogs

Abstract Cytometric analysis was performed in order to detect the expression of surface molecules CD11b and CD11/18 on lymphocytes, granulocytes, and monocytes of peripheral blood of dogs with atopic dermatitis (AD) complicated by purulent dermatitis. The percentage of peripheral blood lymphocytes with CD11b and CD11/18 was found to be significantly higher (P < 0.05) in dogs with AD than in healthy dogs. The percentage of granulocytes expressing CD11b molecule was significantly lower in dogs with complicated AD compared to healthy dogs and dogs with uncomplicated AD. The expression of CD11b and CD11/18 integrin on monocytes of dogs with complicated AD depended on the severity of symptoms and was higher in dogs with deep purulent dermatitis. It was concluded that the expression of CD11b and CD11/18 integrin on leukocytes of dogs with AD depends on the stage of the disease.

scholarly journals Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

2017 ◽  
Vol 12 (2) ◽  
pp. 107
Author(s):  
Dwi Ramadhani ◽  
Sri Sardini ◽  
Masnelli Lubis ◽  
Mukh Syaifudin
Keyword(s):  
Radiation Exposure ◽  
Peripheral Blood ◽  
Healthy Adult ◽  
Background Radiation ◽  
Peripheral Blood Lymphocytes ◽  
Small Sample ◽  
Control Group ◽  
Sample Number ◽  
Blood Lymphocytes ◽  
Study Results

Botteng Village in Mamuju, West Sulawesi was known for the high natural background radiation exposure. Botteng Village inhabitants exposed to high natural radiation in their daily life. Radiation exposure can inhibit the mitosis mechanism at various phases. Our previous study revealed that mitotic and nuclear division indexes in Botteng Village inhabitants were lower compared to control samples. To validate our previous study results here we evaluate the binucleate index in peripheral blood lymphocytes of Botteng Village inhabitants. Blood samples were collected from thirteen healthy adult subjects in Botteng Village and thirteen healthy adult subjects in normal background radiation area. Binucleate index was calculated as the proportion of binucleated cell (BNC) in 500 cells for each sample. Our study showed that the BI in Botteng Village was higher compared to control group (23.58 ± 9.60 vs 23.47 ± 6.24). Statistical analysis revealed that the different was not significant (p=0.973). It is possible that the small sample numbers used in this study were not adequate to represent the BI value in Botteng Village inhabitants. This study also showed that there was insignificant difference of BI in respect to gender and age for all samples. Further study using larger sample number should be conducted to ensure the possibility of BI to evaluate the effect of chronic low radiation dose exposure on lymphocytes proliferation.

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