Relationship between indicators of insulin resistance and oxidative balancein adolescents with androgen deficiency

2021 ◽  
pp. 29-33
Author(s):  
Yu. V. Volkova ◽  
D. A.  Kashkalda ◽  
L. L. Sukhova ◽  
H. V. Kosovtsova ◽  
K. V. Sharun
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Homa Index ◽  
Androgen Deficiency ◽  
Active Compounds ◽  
Testosterone Levels ◽  
Oxidative Balance ◽  
Gpx Activity

The purpose of the work was to study the relationship between the indicators of insulin resistance (IR), free radical oxidation products (FRO) levels, and the antioxidant system activity in adolescents with androgen deficiency (AD).Materials and methods. 58 adolescents 13­—18 years old with AD were examined. Serum levels of total testosterone, glucose, insulin, tiobarbituric acid active compounds (TBA), carbonylated proteins (CB), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) activity were determined. The HOMA index and the coefficient of oxidative stress were calculated.Results and discussion. In the group of adolescents with AD without IR, an inverse correlation was found between the HOMA index and CAT activity. CAT activity negatively correlated with CB content and glucose concentration. In addition, an inverse relationship was found between the activity of GPO and SOD. IR indices indirectly, through feedback with the activity of CAT, influence to the formation of conditions for inhibition/activation of FRO of proteins.IR was revealed in 37.9 % of the examined adolescents with AD. Direct relationship was recorded between IR indicators and markers of oxidative stress, as well as between testosterone levels and GPx activity in the group of these patients. Direct correlation between the coefficient of oxidative stress and the content of TBA-­active compounds indicates the formation of oxidative stress due to the activation of lipid peroxidation. Reduced testosterone levels in adolescents with AD inhibits an increase in GPx activity and prevents compensation for excessive intensity of free radical processes. Conclusion. The results indicate that adolescents with AD have a close relationship between the processes of carbohydrate metabolism, FRO, and antioxidant protection.In adolescents with AD without IR, a balancebetween the studied parameterswas found.The formation of IR in adolescents with AD shifts the equilibrium of the oxidative balance towards the activation of FRO processes. Decreased testosterone levels in adolescents with AD do not maintain the body’s antioxidant status within normal limits.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

scholarly journals PSX-3 Antioxidant system characteristics in Saanen goats depending on performance

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 461-462
Author(s):  
Polina D Bokhan ◽  
Alesya A Bakhta ◽  
Larisa Y Karpenko ◽  
Anna I Kozitcyna ◽  
Anna B Balykina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Milk Yield ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Dairy Goat ◽  
Blood Levels ◽  
Lactation Performance ◽  
Radical Oxidation ◽  
System Characteristics

Abstract Dairy goat breeding is widespread worldwide. Goat milk and other derivative products are an important part of nutrition. Dairy products are the most important part of goat breeding; therefore, lactation performance is a relevant issue. The purpose of this study was to evaluate antioxidant system characteristics in Saanen goats depending on lactation performance. The experiment was conducted in the north-western region of Russian Federation, in the laboratories of the biochemistry and physiology departments at FSBEI of Higher Education «SPbSAVM». The three experimental groups included 30 Saanen goats each, 2nd–3rd lactations, selected using matched pairs method. 1st group included low milk producing ability goats (< 600 kg of milk yield per year), 2nd group included average milk producing ability goats (600–800 kg of milk yield per year), 3rd group included high milk producing ability goats (>800 kg of milk yield per year). The blood samples were taken once: at peak lactation performance (45 days after parturition). The blood levels of lipid peroxygenation markers (malondialdehyde, dienketone and conjugated dienes) and superoxide dismutase and catalase activity were assessed by standard methods. Results presented as mean±standard error of the mean. Student’s t-test was used after proving normal distribution. Level of significance is labeled as P < 0.05. The results are displayed in table 1 and 2. After analyzing the received data of antioxidant system characteristics, we found a high intensity of free-radical oxidation in high milk producing ability goats. This results in oxidative stress development. Activity of anti-oxidizing enzymes was elevated; therefore, free-radical oxidation is intensive. Consequently, these data allow us to take into consideration exogenous anti-oxidizing agents administration in high milk producing ability goats in order to reduce oxidative stress.

The influence of humus acids of peloids and its components on free radical processes

2018 ◽  
Vol 64 (5) ◽  
pp. 429-432 ◽  
Author(s):  
N.P. Avvakumova ◽  
F.H. Kamilov ◽  
A.V. Zhdanova ◽  
I.A. Men’shikova ◽  
Yu.V. Zhernov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Albino Rats ◽  
Redox Systems ◽  
Radical Oxidation ◽  
The Third ◽  
Glutathione Peroxidases ◽  
Humus Acids ◽  
Radical Processes

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Humic acids restore not only normal physiological redox systems, but also increase the activity of antioxidant enzymes on the 10th day.

scholarly journals Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes

2021 ◽  
Vol 11 (3) ◽  
pp. 12-17
Author(s):  
A. V. Elikov
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Former Athletes ◽  
Oxidative Balance

Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent.

scholarly journals Free Radical Oxidation and Sleep Disorders in Andro- and Menopause (Literature Review)

2020 ◽  
Vol 5 (1) ◽  
pp. 31-41
Author(s):  
N. V. Semenova ◽  
I. M. Madaeva ◽  
L. I. Kolesnikova
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Sleep Deprivation ◽  
Sleep Disorders ◽  
Sleep Apnea Syndrome ◽  
Free Radical Oxidation ◽  
Carbonyl Stress ◽  
Apnea Hypopnea Index ◽  
Radical Oxidation ◽  
Obstructive Sleep

This review presents data on changes in the physiology of sleep during reproductive aging. It is noted that insomnia and obstructive sleep apnea syndrome (OSAS) are the main sleep disorders. The results of foreign and domestic studies in the field of free radical oxidation during sleep deprivation in animal models are presented, indicating the dependence of processes on the duration of sleep deprivation. The largest number of studies of free radical processes in a person with somnological pathology was carried out in the study of OSAS. Blood, urine, saliva, condensate of exhaled air can be biomaterial for determining the parameters of free radical oxidation. It was shown that the intensity of oxidative stress depends on the severity of OSAS, as evidenced by the positive correlation of the level of active products of thiobarbituric acid, the products of oxidation of proteins and carbonyl groups with the apnea/hypopnea index, determining the development of not only oxidative, but also carbonyl stress in patients with a severe degree OSAS. Biomarkers such as thioredoxin, malondialdehyde, superoxide dismutase, and reduced iron have shown a more stable relationship between increased oxidative stress and OSA. Despite the results obtained, the question of the association of oxidative stress and hypoxia in OSA remains debatable, which is associated with the opposite results of some studies. Insomnia, which occurs mainly in females, is accompanied by a high level of end products of lipid peroxidation with a decrease in the activity of antioxidants such as paraoxonase, an enzymatic component of the glutathione system. Along with this, menopausal women present low levels of uric acid, which correlates with high scores of the Pittsburgh sleep quality index questionnaire. Recent studies have identified an association between the activity of the «lipoperoxidation – antioxidants» system and the Clock 3111T/C gene polymorphism in menopausal Caucasian women, indicating the protective role of the minor allele.

scholarly journals Oxidative Stress and Free-Radical Oxidation in BCG Granulomatosis Development

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Elena Menshchikova ◽  
Nikolay Zenkov ◽  
Victor Tkachev ◽  
Oksana Potapova ◽  
Liliya Cherdantseva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Granulomatous Inflammation ◽  
Free Radical Oxidation ◽  
Peritoneal Exudate ◽  
Numerical Density ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Bcg Granulomatosis ◽  
Tuberculous Granuloma

Background. Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice.Methods. Chronic granulomatous inflammation was induced in male BALB/c mice by intravenously injecting the BCG vaccine, and the production of oxidative stress (activity of free-radical oxidation processes) and histological changes in the lungs, liver, and peritoneal exudate were measured 3, 30, 60, and 90 days after infection.Results. The tuberculous granuloma numerical density and diameter continuously increased from day 30 to day 90, and the macrophage content within the granulomas progressively diminished with a concomitant elevation in the number of epithelioid cells. The activity of the free-radical oxidation processes in the liver (i.e., the intensity of the homogenate chemiluminescence) reached a maximum at postinfection day 60 and subsequently began to decrease. The peak generation of reactive oxygen species by phagocytes in the peritoneal exudate (measured using flow cytometry) was also shifted in time and fell on day 30.Conclusions. The rise in the steady-state concentration of H2O2in the liver of mice with BCG-induced granulomatosis is not related to local H2O2production by phagocytes, and a decrease in the severity of generalized inflammation precedes the resolution of local inflammation.

Changes in free-radical oxidation in isolated femoral fracture during the systemic ozone therapy

2021 ◽  
pp. 79-85
Author(s):  
К.С. Абрамов ◽  
Е.В. Давыдова ◽  
М.В. Осиков ◽  
О.И. Огнева ◽  
А.И. Синицкий
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Surgical Treatment ◽  
Femoral Fracture ◽  
Plasma Lipid ◽  
Free Radical Oxidation ◽  
Lipid Extract ◽  
Ozone Therapy ◽  
Radical Oxidation ◽  
End Products

Введение. Изолированные переломы бедренной кости (ИПБК) составляют более 10% от числа всех дорожных травм. Реакция организма в ответ на травму сопровождается нарушением гомеостаза, активацией процессов свободно-радикального окисления. Одним из перспективных направлений коррекции метаболических нарушений при хирургической патологии является озонотерапия. Цель исследования - оценка в динамике показателей свободно-радикального окисления в плазме крови у пациентов с изолированным переломом бедренной кости в условиях применения системной озонотерапии. Методика. Исследование выполнено на 36 пациентах с ИПБК и 20 условно здоровых лицах. Содержание изопропанол- и гептан-растворимых первичных, вторичных и конечных продуктов перекисного окисления липидов (ПОЛ) в плазме оценивали на 5-7-е сут после травмы до начала хирургического лечения, а также на 18-е сут после травмы, хирургического лечения и стандартного курса терапии, в том числе дополненного курсом малой аутогемотерапии (МАГТ), - 7-9 инъекций с озонированием крови (концентрация озона 20 мг/л). Результаты. Ранний посттравматический период у пациентов с ИПБК до оперативного лечения сопровождается накоплением первичных (диеновые конъюгаты), вторичных (ацилгидроперекиси, кетодиены и сопряженные триены) и конечных (основания Шиффа) продуктов ПОЛ в гептановой фазе липидного экстракта плазмы, а также вторичных и конечных продуктов ПОЛ в изопропанольной фазе липидного экстракта плазмы. У пациентов с ИПБК после оперативного лечения на фоне стандартной консервативной терапии сохраняется высокий уровень продуктов ПОЛ в гептановой и изопропанольной фазах липидного экстракта плазмы, что свидетельствует об эскалации окислительного стресса. Применение процедуры МАГТ с озонированием крови приводит к снижению и полному восстановлению уровня продуктов ПОЛ в изопропанольной фазе, снижению и частичному восстановлению продуктов ПОЛ в гептановой фазе липидного экстракта плазмы. Заключение. Применение МАГТ с озонированием крови оптимизирует состояние про- и антиоксидантных систем крови и ограничевает эскалацию окислительного стресса. Introduction. Isolated femoral fractures (IFF) account for more than 10% of all road injuries. The body responds to trauma with homeostatic disorders and activation of free radical oxidation. The ozone therapy is one of promising directions for correction of metabolic disorders in surgical pathology due to the pleiotropic effects of reactive oxygen species. The aim of the study was to evaluate changes in indexes of free radical oxidation in plasma of patients with isolated femoral fracture during the systemic ozone therapy. Methods. The study included 36 patients with IFF and 20 healthy individuals. Plasma concentrations of isopropanol and heptane-soluble primary, secondary, and end products of lipid peroxidation (LPO) were measured at 5-7 days after the injury, prior to the surgical treatment, and at 18 days after the injury following the surgical treatment and a standard therapy, including a course of minor autohemotherapy (7-9 injections of ozonated blood with ozone concentration of 20 mg/l). Results. The early post-traumatic period preceding the surgical treatment was associated with accumulation of LPO primary (conjugated dienes), secondary (ketodienes, and conjugated trienes) and end (Schiff bases) products in the heptane phase of plasma lipid extract, as well as LPO secondary and end products in the isopropanol phase of plasma lipid extract. In patients with IFF after the surgical treatment in combination with a standard, conservative therapy, high levels of LPO products remained in the heptane and isopropanol phases of plasma lipid extract, which indicated escalation of oxidative stress. The use of minor autohemotherapy with blood ozonation resulted in a decrease and complete restoration of the level of LPO products in the isopropanol phase and a decrease and partial return to the normal level of LPO products in the heptane phase of plasma lipid extract. Conclusions. The use of minor autohemotherapy with blood ozonation improved the condition of pro- and antioxidant blood systems and restricted the escalation of oxidative stress.

scholarly journals FEATURES OF FREE SKIN LIPID HOMOGENATES IN RATS AT DIFFERENT WAYS OF WOUND CLOSURE

2016 ◽  
Author(s):  
Ye. O. Loza ◽  
M. I. Marushchak ◽  
O. V. Denefil
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Wound Closure ◽  
Free Radical Oxidation ◽  
Skin Lipid ◽  
Radical Oxidation ◽  
Medical Institutions ◽  
Biological Glue ◽  
Skin Homogenate ◽  
Oxidation System

According to different authors from 4 % to 22 % of patients who applied to medical institutions have abnormal scars. The aim of our research was to identify the characteristics of oxidative stress in skin homogenate of rats with different methods of wound closure. Analysis of all indicators allows to assert that intensity of free radical oxidation in the skin of the animals after applying skin glue is much lower compared to the animals after suturing. In furtherresearch is planned to analyze indicators of free radical oxidation system antioxidation protection and reparative ability of the skin when used sutures and biological glue in rats with the diabetes.

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