Calcium-dependent complications of lactase deficiency in patients with type 2 diabetes mellitus

2021 ◽  
Author(s):  
A. B. Andrusha
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Bone Resorption ◽  
Bone Tissue ◽  
Bone Remodelling ◽  
Osteoporotic Fractures ◽  
Lactase Deficiency ◽  
X Ray

Objective — to assess the degree of osteodeficiency and probability of osteoporotic fractures in patients with type 2 diabetes mellitus in the absence or presence of lactase deficiency. Materials and methods. All examined patients with type 2 diabetes mellitus were divided into 2 groups depending on the presence/absence of lactase deficiency. In addition to routine examination methods, specific methods were used for diagnosing lactase deficiency, assessing bone mineral density (using dual‑energy X‑ray absorptiometry) and bone quality (ultrasound densitometry), the state of bone remodelling (according to markers of bone resorption and formation), probability of osteoporotic fractures (using FRAX and QFracture calculators), dietary and lifestyle habits were also studied. Results. The changes have been revealed in both processes of bone remodelling — increased bone resorption and insufficient bone formation, and the activity of bone formation, which was the lowest in patients with lactase deficiency and type 2 diabetes mellitus. The results of X‑ray absorptiometry confirmed that osteoporosis was significantly more often in patients with type 2 diabetes mellitus in the presence of lactase deficiency. The use of ultrasonic densitometry confirmed the violation of bone tissue micro architectonics. The indicator of broadband ultrasound attenuation, which reflects the qualitative characteristics of bone tissue, was the lowest in patients with type 2 diabetes mellitus accompanied by lactase deficiency. The probability of osteoporotic fractures according to the results of the assessment with the online calculator FRAX® was higher than the average risk in both groups of patients. No significant difference was established in this indicator between these groups of patients in contrast to the risk calculated with the QFracture instrument — it was the highest in patients with lactase deficiency. Conclusions. The presence of lactase deficiency in patients with type 2 diabetes mellitus can be considered as a factor that contributes to the development of osteodeficiency, deterioration of the quality of bone tissue, imbalance in bone remodelling and an increase in the probability of osteoporotic fractures.  

scholarly journals Immunohistochemical features of the bone tissue of the lower extremities in patients with type 2 diabetes mellitus

2022 ◽  
Vol 24 (5) ◽  
pp. 448-455
Author(s):  
A. Yu. Tokmakova ◽  
E. A. Kogan ◽  
E. L. Zaitseva ◽  
S. A. Demura ◽  
N. V. Zharkov ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Bone Resorption ◽  
Bone Tissue ◽  
Immunohistochemical Study ◽  
Control Group ◽  
Diabetic Neuroosteoarthropathy ◽  
Group 1

Background: Diabetic neuroosteoarthropathy is a serious disabling complication of diabetes mellitus, which, in the absence of timely correct treatment, can lead to high amputations of the affected limb. At present, the reasons and mechanism of the development of Charcot’s foot are not completely clear. It is extremely important to determine the pathophysiological mechanisms of DNOAP formation and to search for reliable markers-predictors of this pathology.Aim: To study the immunohistochemical characteristics of the bone tissue of the lower extremities in patients with diabetic neuroosteoarthropathy in comparison with patients with diabetes mellitus without this pathology.Materials and methods: During the foot surgery, a bone fragment of the foot was harvested for immunohistochemical study of receptor markers for PINP, PIIINP, and RAGE in the group of patients with DNOAP compared with the control group.Results: The study included 20 patients with type 2 diabetes mellitus and were divided into 2 groups: 10 patients with DNOAP made up group 1, 10 patients without DNOAP — group 2.Patients in both groups were comparable in AGE, experience with type 2 diabetes, and glycemic control.During the immunohistochemical study, a significant increase in the staining intensity of receptor markers for PINP, PIIINP, and AGE was recorded in the group of patients with DNOAP compared with the control group (p <0.05).Conclusion: For the first time, an immunohistochemical study of markers of bone resorption and AGE was carried out in persons with DNOAP. The results obtained indicate impaired collagen formation and, as a consequence, impaired bone formation and bone resorption in patients with DNOAP: in group 1, a statistically significant increase in the expression of PINP, PIIINP, and RAGE was revealed.

scholarly journals X-Ray Absorption Spectroscopy Measurements of Cu-ProIAPP Complexes at Physiological Concentrations

2019 ◽  
Vol 4 (1) ◽  
pp. 13 ◽  
Author(s):  
Emiliano De Santis ◽  
Emma Shardlow ◽  
Francesco Stellato ◽  
Olivier Proux ◽  
Giancarlo Rossi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Absorption Spectroscopy ◽  
Amyloid Fibrils ◽  
High Energy ◽  
Islet Amyloid ◽  
X Ray ◽  
X Ray Absorption

The amyloidogenic islet amyloid polypeptide (IAPP) and the associated pro-peptide ProIAPP1–48 are involved in cell death in type 2 diabetes mellitus. It has been observed that interactions of this peptide with metal ions have an impact on the cytotoxicity of the peptides as well as on their deposition in the form of amyloid fibrils. In particular, Cu(II) seems to inhibit amyloid fibril formation, thus suggesting that Cu homeostasis imbalance may be involved in the pathogenesis of type 2 diabetes mellitus. We performed X-ray Absorption Spectroscopy (XAS) measurements of Cu(II)-ProIAPP complexes under near-physiological (10 μM), equimolar concentrations of Cu(II) and peptide. Such low concentrations were made accessible to XAS measurements owing to the use of the High Energy Resolved Fluorescence Detection XAS facility recently installed at the ESRF beamline BM16 (FAME-UHD). Our preliminary data show that XAS measurements at micromolar concentrations are feasible and confirm that ProIAPP1–48-Cu(II) binding at near-physiological conditions can be detected.

scholarly journals Bioelectrical Impedance and Dual-Energy X-Ray Absorptiometry Assessments of Changes in Body Composition Following Exercise in Patients with Type 2 Diabetes Mellitus

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Masae Miyatani ◽  
Pearl Yang ◽  
Scott Thomas ◽  
B. Catharine Craven ◽  
Paul Oh
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Body Composition ◽  
Bioelectrical Impedance ◽  
Dual Energy ◽  
Fat Free Mass ◽  
Post Intervention ◽  
X Ray

We aimed to compare the level of agreement between leg-to-leg bioelectrical impedance analysis (LBIA) and dual-energy X-ray absorptiometry (DXA) for assessing changes in body composition following exercise intervention among individuals with Type 2 diabetes mellitus (T2DM). Forty-four adults with T2DM, age53.2±9.1years; BMI30.8±5.9 kg/m2participated in a 6-month exercise program with pre and post intervention assessments of body composition. Fat free mass (FFM), % body fat (%FM) and fat mass (FM) were measured by LBIA (TBF-300A) and DXA. LBIA assessments of changes in %FM and FM post intervention showed good relative agreements with DXA variables (P<0.001). However, Bland-Altman plot(s) indicated that there were systematic errors in the assessment of the changes in body composition using LBIA compared to DXA such that, the greater the changes in participant body composition, the greater the disparity in body composition data obtained via LBIA versus DXA data (FFM,P=0.013; %FM,P<0.001; FM,P<0.001). In conclusion, assessment of pre and post intervention body composition implies that LBIA is a good tool for assessment qualitative change in body composition (gain or loss) among people with T2DM but is not sufficiently sensitive to track quantitative changes in an individual’s body composition.

Bone mass and bone resorption in postmenopausal women with type 2 diabetes mellitus

Metabolism ◽  
2008 ◽  
Vol 57 (7) ◽  
pp. 940-945 ◽  
Author(s):  
Hiroko Hosoda ◽  
Michiaki Fukui ◽  
Ichiko Nakayama ◽  
Mai Asano ◽  
Mayuko Kadono ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Bone Resorption ◽  
Bone Mass ◽  
Postmenopausal Women

scholarly journals STATE OF PARADONT IN RATS AFTER IMPLANTOLOGICAL OPERATION ON THE BACKGROUND OF SUGAR DIABETES 2 TYPE

2017 ◽  
Vol 21 (6) ◽  
pp. 313-316
Author(s):  
Svetlana Aleksandrovna Dem'yanenko ◽  
M. G Drobyazko ◽  
P. D Rozhko
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Alkaline Phosphatase ◽  
Type 2 Diabetes Mellitus ◽  
Acid Phosphatase ◽  
Bone Tissue ◽  
Pathological Condition ◽  
Dental Implantation

In rats with experimental (protamine) type 2 diabetes mellitus (DM2), the level of lysozyme and catalase decreases, the activity of urease, the degree of dysbiosis and the activity of elastase increase. Dental implantation on the background of CD2 aggravates the pathological condition of the gums. In the periodontal bone tissue of rats with CD2, a decrease in the activity of alkaline phosphatase (ACF) and an increase in the activity of acid phosphatase (CF), which reduces the mineralizing index (FF/CF) by more than 2.5 times. An increase in the activity of elastase in bone tissue by 36% indicates the development of inflammation. Dental implantation on the background of CD2 does not improve the condition of the periodontal bone tissue.

scholarly journals Condition of bone tissue in postmenopausal women with type 2 diabetes mellitus of Buryat population

2020 ◽  
Vol 22 (5) ◽  
pp. 436-443
Author(s):  
Tatiana P. Bardymova ◽  
Maksim V. Mistiakov ◽  
Galina P. Yagelskaya
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Postmenopausal Women ◽  
Bone Tissue ◽  
Bone Remodeling ◽  
Comparison Group ◽  
Russian Population ◽  
Type 2 Dm

BACKGROUND: In most countries, there is a rapid increase in the population of patients with type 2 Diabetes Mellitus (DM). Bone changes in postmenopausal women with type 2 DM are associated with increased bone mineral density (BMD). The study of metabolic processes in bone tissue in comorbid pathology in different ethnic groups is continuing. AIMS: To study the concentration of markers of bone remodeling and indicators of BMD in postmenopausal women with type 2 DM of the Buryat population. MATERIALS AND METHODS: Thirty-nine postmenopausal women with type 2 DM (22 Russian population and 17 Buryat population) were examined. The comparison group consisted of 42 postmenopausal women (21 Russian population and 21 - Buryat population). The study of BMD in the lumbar spine (L1-L4), the femoral neck (Neck), and the proximal femur (Total hip) was performed using dual-energy X-ray absorptiometry. Parameters of osteocalcin (OC), type 1 N-terminal procollagen propeptide (P1NP), C-terminal telopeptides of type I collagen (-Cross laps), 25(OH) vitamin D and ionized calcium were evaluated. RESULTS: The presented study revealed a simultaneous increase in osteosynthesis: ОС (p=0.048) and P1NP (p=0.016) and in the bone resorption marker -Cross laps (p=0.020) accompanied by the absence of changes in BMD in women with type 2 DM in the postmenopausal period of the Buryat population relative to women with type 2 DM in the postmenopausal state of the Russian population. A decrease in osteosynthesis parameters (ОC, p=0.021; P1NP, p=0.029) with an increase in BMD L1-L4 (p=0.024) and BMD Total hip (p=0.039) in postmenopausal women with type 2 DM of the Buryat population was found relative to the women of the Buryat population in comparison group. CONCLUSIONS: The state of bone tissue in postmenopausal women with type 2 DM of the Buryat population is characterized by the activation of bone remodeling processes.

scholarly journals EXPERIMENTAL SUBSTANTIATION OF PARODONTROTECTOR EFFICIENCY OF THE COMPLEX OF DRUGS IN RATS WITH TYPE 2 DIABETES DURING ORTHODONTIC TEETH RELOCATION

2020 ◽  
Vol 16 (3) ◽  
pp. 46-52
Author(s):  
Svetlana Dem'yanenko ◽  
Marina Morozova ◽  
Andrey Morozov ◽  
Anastasiya Denga ◽  
Olga Makarenko
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Bone Tissue ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Protective Effects ◽  
Experimental Animals ◽  
Patients With Diabetes

Introduction. Pathological changes in the periodontium in people with diabetes mellitus, according to some data, are aggravated after orthodontic intervention, which dictates the need to carry out preventive measures, the effectiveness of which will determine the quality of orthodontic treatment. The aim of this study was to study the periodontal protective effect of two domestic antidisbiotic drugs containing plant extracts, vitamins, calcium and phosphorus salts. Methodology. The experiments were carried out on white Wistar rats, divided into 5 groups. The gums and periodontal bone tissue were isolated. The activity of urease, lysozyme and the content of hyaluronic acid was determined in the gingival homogenate, and the degree of dysbiosis was calculated. In the homogenate of the periodontal bone tissue, the activity of alkaline and acid phosphatases was determined, the mineralization index and the degree of mineralization, as well as the content of calcium and protein, were calculated. Results. The obtained results of our study have convincingly proved the pronounced periodontal protection efficacy of antidisbiotic drugs for oral applications of the plates "CM-2" and the drug Enoant polyphenols. The proposed therapeutic and prophylactic complex inhibits inflammation, destruction of bone tissue and the development of dysbiosis, along with the manifestation of pronounced adaptogenic, antimicrobial and periodontal protective effects in experimental animals against the background of the development of type 2 diabetes mellitus and orthodontic tooth movement. Conclusion. The proposed therapeutic and prophylactic complex inhibits inflammation, destruction of bone tissue and the development of dysbiosis in experimental animals against the background of the development of type 2 diabetes mellitus and orthodontic tooth movement. The obtained results of the experimental study serve as the basis for the recommendation of the investigated complex, including the local application of plates with herbal extracts, vitamins, calcium and microelements, along with the systemic use of grape polyphenol concentrate, in an orthodontic clinic for patients with diabetes mellitus.

scholarly journals RESULTS OF CLINICAL STUDY OF COMPLEX TREATMENT OF GENERALIZED PERIODONTITIS WITH THE USE OF STRONTIUM DRUGS IN PATIENTS WITH TYPE 2 DIABETES MELLITUS

2021 ◽  
pp. 37-43
Author(s):  
A. V. Samoilenko ◽  
L. M. Matvyeyenko
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Bone Resorption ◽  
Strontium Ranelate ◽  
Alveolar Bone ◽  
Main Group ◽  
Complex Treatment ◽  
Periodontal Tissues

The most significant periodontal disorders associated with diabetes mellitus are due to changes in bone tissue. It has become necessary for specific osteotropic therapy that can normalize metabolic processes in the alveolar bone. In turn, currently the most promising in terms of improving osteogenic activity are strontium ions. Strontium ranelate is used to treat osteoporosis due to its antiresorptive and osteoanabolic action. However, its effectiveness against alveolar bone has not been sufficiently studied. The purpose of the work is to conduct a clinical study of complex treatment of generalized periodontitis, supplemented with strontium ranelate, in patients with type 2 diabetes mellitus. Materials and methods of the research. The study included 60 patients with generalized periodontitis of I-II degree of severity, chronic course, aged 35-45 years. Type 2 diabetes mellitus was diagnosed and two groups were formed. The traditional treatment regimen was used in the group of comparison (main group) where Strontium Ranelate was prescribed additionally. The treatment was evaluated according to the dynamics of clinical observations, orthopantomography and computed tomography data, the results of biochemical studies. As markers of bone resorption, tartrate-resistant acid phosphatase (TRAP) activity was determined in blood serum and the content of β-CrossLaps fragments was determined in urine. As markers of osteogenesis, the concentration of C-terminal propeptide type I procollagen (CICP) was detected in blood plasma, bone alkaline phosphatase (BAP) and osteocalcin were detected in serum. Serum parathyroid hormone concentrations, total blood calcium and total inorganic phosphorus in the blood were studied as indicators of mineral metabolism. Results of the research. In the earliest possible timeframe the complex treatment of generalized periodontitis was conducted during the observation which led to clinical stabilization of the inflammatorydestructive process in the periodontal tissues without a significant difference between the experimental groups (p ˃ 0.05). However, the condition of periodontal tissues in patients of the experimental groups differed in a year after treatment. In 16.7% of patients from the comparison group, recurrence of the inflammatory-destructive process in periodontal tissues was diagnosed, while the cases the deterioration of the pathological process was not detected in the main group. A significant difference was found for complex periodontal indices (Ramfjord, PI and SPITN) (p <0.05). Clinical and radiological stabilization was observed in 83.3% of patients of the comparison group and in 100% in the main group. According to the results of computed tomography of the alveolar bone, an increase in bone mineral density was established in both groups, but only in the main group the difference between indices before and after treatment was significant (p˂0.05). In patients of the main group a more pronounced decrease in the activity of tartrate-resistant acid phosphatase (TRAP) was found in the serum and the concentration of β-CrossLaps was found in the urine, indicating inhibition of bone resorption, as well as markers of bone formation the concentration of C-terminal propeptide (CICP) was found in blood plasma, bone alkaline phosphatase (BAP) was found in serum, osteocalcin (p <0.05). Indicators of mineral metabolism in bone tissue, both during treatment and for experimental groups, almost did not differ (p> 0.05). Thus, the use of strontium drugs in the complex treatment of generalized periodontitis in patients with type 2 diabetes mellitus provides a longer and more stable clinical and radiological stabilization of the pathological process in periodontal tissues, primarily by inhibiting bone resorption and enhancing osteogenesis. So, they can be recommended for the wide use in stomatological practice.

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