scholarly journals Genetic diversity analysis in tossa jute (Corchorus olitorius L.) germplasm lines

2018 ◽  
Vol 10 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Jawahar Lal Jatothu ◽  
A. Anil Kumar ◽  
S. B. Choudhary ◽  
H. K. Sharma ◽  
R. T. Maruthi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phenotypic Diversity ◽  
Diversity Analysis ◽  
Corchorus Olitorius ◽  
Basal Diameter ◽  
Mean Values ◽  
Genetic Diversity Analysis ◽  
Eigen Values ◽  
Cluster Distance ◽  
Fibre Yield

An experiment was conducted to examine the magnitude of genetic diversity and characters contributing to genetic diversity among 81 tossa jute genotypes. Cluster analysis based on Euclidean squared distances and ward’s method, the genotypes were grouped into ten distinct clusters. Analysis reveals that stick weight contributes maximum to the phenotypic diversity (65.52%) followed by green weight (13.64%) and fibre yield (10.10%). Among the clusters Cluster IX recorded highest mean fibre yield (19.91g) followed by Cluster VII (18.94g) and these clusters also recorded high mean values for plant height, basal diameter, green weight and stick weight. The highest inter- cluster distance was 186.80 (between clusters II and X) followed by 161.26 (between clusters IV and X), indicating the wide genetic diversity among these clusters. The highest intra-cluster distance was observed in cluster II (20.34) and the lowest in cluster X (3.17). The average inter-cluster distances were higher than the average intra-cluster distances, which shows the presence of wide genetic diversity among the genotypes of different clusters than those of the same cluster. The first two principal components, whose Eigen values are greater than one, accounted for 74% of the total variation among the five characters. The information obtained from diversity analysis is useful in planning further breeding programme for tossa jute improvement.

scholarly journals An RNA Sequencing Transcriptome Analysis and Development of EST-SSR Markers in Chinese Hawthorn through Illumina Sequencing

Forests ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 82 ◽  
Author(s):  
Suliya Ma ◽  
Wenxuan Dong ◽  
Tong Lyu ◽  
Yingmin Lyu
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gene Diversity ◽  
Low Cost ◽  
Polymorphism Analysis ◽  
Diversity Analysis ◽  
Large Set ◽  
Mean Values ◽  
Genetic Diversity Analysis ◽  
Geographical Regions

Chinese hawthorn (Crataegus pinnatifida) is an important ornamental and economic horticultural plant. However, the lack of molecular markers has limited the development and utilization of hawthorn germplasm resources. Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) allow precise and effective cultivar characterization and are routinely used for genetic diversity analysis. Thus, we first reported the development of polymorphic EST-SSR markers in C. pinnatifida with perfect repeats using Illumina RNA-Seq technique. In total, we investigated 14,364 unigenes, from which 5091 EST-SSR loci were mined. Di-nucleotides (2012, 39.52%) were the most abundant SSRs, followed by mono- (1989, 39.07%), and tri-nucleotides (1024, 20.11%). On the basis of these EST-SSRs, a total of 300 primer pairs were designed and used for polymorphism analysis in 70 accessions collected from different geographical regions of China. Of 239 (79.67%) pairs of primer-generated amplification products, 163 (54.33%) pairs of primers showed polymorphism. Finally, 33 primers with high polymorphism were selected for genetic diversity analysis and tested on 70 individuals with low-cost fluorescence-labeled M13 primers using capillary electrophoresis genotyping platform. A total of 108 alleles were amplified by 33 SSR markers, with the number of alleles (Na) ranging from 2 to 14 per locus (mean: 4.939), and the effective number of alleles (Ne) ranging from 1.258 to 3.214 (mean: 2.221). The mean values of gene diversity (He), observed heterozygosity (Ho), and polymorphism information content (PIC) were 0.524 (range 0.205–0.689), 0.709 (range 0.132–1.000), and 0.450 (range 0.184–0.642), respectively. Furthermore, the dendrogram constructed based on the EST-SSR separated the cultivars into two main clusters. In sum, our study was the first comprehensive study on the development and analysis of a large set of SSR markers in hawthorn. The results suggested that the use of NGS techniques for SSR development represented a powerful tool for genetic studies. Additionally, fluorescence-labeled M13 markers proved to be a valuable method for genotyping. All of these EST-SSR markers have agronomic potential and constitute a scientific basis for future studies on the identification, classification, and innovation of hawthorn germplasms.

scholarly journals Genetic Diversity Analysis by D2 Analysis in Fine Scented Genotypes of Rice (Oryza sativa L.)

2021 ◽  
Vol 10 (11) ◽  
pp. 48-55
Author(s):  
V. Netam S. K. Sinha ◽  
K. Tigga V. K. Singh ◽  
N. Chouksey
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Cluster Distance

The present investigation on “Diversity analysis by D2 analysis in fine scented genotypes of rice (Oryza sativa L.)” was used to investigate the diversity among 40 fine scented genotypes obtained from the Indira Gandhi Krishi Vishwavidyalaya in Raipur. The current studies was conducted at research cum instructional farm, IGKV, RMD Ambikapur, Chhattisgarh. The experiment was conducted in RBD with purpose to characterized 40 genotypes of rice along with 4 checks viz. CG Sugandhitbhog, CG Devbhog, Indira Sugandhit Dhan-1 and Dubrajsel 1 for diversity. Based on cluster analysis, the genotypes were grouped into 5 clusters in which cluster I was the largest consistin of 29 genotypes. While cluster IV & V were the smallest with only a single genotypes; each. Maximum intra cluster distance was found in the cluster II, Which comprises only 5 genotypes. The most divergent clusters observed were cluster III & V. The minimum cluster distance was recorded between cluster I & III.

scholarly journals Genetic Diversity Analysis In Brassica rapa Using Morphological Characters

2013 ◽  
Vol 38 (1) ◽  
pp. 11-18
Author(s):  
N Jahan ◽  
SR Bhuiyan ◽  
MZA Talukder ◽  
MA Alam ◽  
M Parvin
Keyword(s):  
Genetic Diversity ◽  
Field Experiment ◽  
Brassica Rapa ◽  
Broad Spectrum ◽  
Multivariate Analyses ◽  
Morphological Characters ◽  
Diversity Analysis ◽  
Agronomic Performance ◽  
Genetic Diversity Analysis ◽  
Cluster Distance

A field experiment was conducted in the experimental field of Genetics and Plant Breeding Department, Sher-e Bangla Agricultural University, Dhaka, Bangladesh to study on genetic diversity in 10 F4 lines obtained through intervarietal crosses along with 8 released varieties of Brassica rapa during November 2007 to February 2008. Different Multivariate analyses were performed to classify 18 genotypes. All the genotypes were grouped into four clusters. Cluster IV was the largest comprising of 7 genotypes and cluster II was the smallest with 2 genotypes. Cluster II had the highest intra-cluster distance and Cluster I had the lowest intra cluster distance. Inter cluster distance was maximum (11.697) between clusters II and III. The results revealed that genotypes chosen for hybridization from clusters with highest distances would give high heterotic F1 and broad spectrum of variability in segregating generations. The characters- number of primary branches/plant, number of secondary branches/plant and days to 50% flowering contributed maximum towards divergence among Brassica genotypes. Considering cluster distance, inter genotypic distance and other agronomic performance G2 and G14 from cluster I; G18 from cluster II; G1, G9 and G12 from cluster III and G16 and G17 from cluster IV may be considered as better parents for future uses in hybridization program. Bangladesh J. Agril. Res. 38(1): 11-18, March 2013 DOI: http://dx.doi.org/10.3329/bjar.v38i1.15185

scholarly journals Genetic Diversity Analysis for Yield Parameters in Rice (Oryza sativa. L) Genotypes

2020 ◽  
pp. 52-56
Author(s):  
B. Soundharya ◽  
Ramya Rathod ◽  
Firdoz Shahana ◽  
Y. Swathi ◽  
P. Jalender Naik ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa L ◽  
Flowering Plant ◽  
Research Station ◽  
Diversity Analysis ◽  
Hybrid Vigour ◽  
Genetic Diversity Analysis ◽  
Plot Yield ◽  
Cluster Distance ◽  
Total Divergence

The present investigation was conducted during Kharif, 2019 at Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad. Twenty rice genotypes were studied to assess genetic diversity analysis for days to fifty percent flowering, plant height (cm), ear bearing tillers per m2, panicle length (cm), number of filled grains per panicle, test weight (g) and plot yield (kg/ha). Based on the analysis, the genotypes were grouped into four clusters. The maximum number of genotypes (14 genotypes) was grouped in cluster I. Cluster II and III consists of three and two genotypes respectively. Remaining cluster was represented by a single genotype. Maximum inter cluster distance was observed between I and II (27.46) followed by between cluster I and IV (23.19) and between cluster II and IV (20.43) indicating wider genetic diversity between genotypes. Hence these lines may be utilized in a further breeding programme for the exploitation of hybrid vigour. Among the seven traits studied, the number of filled grains per panicle contributed maximum divergence (42.68%) followed by days to 50% flowering (32.04%), grain yield per plot (12.01%) and ear bearing tillers per m2 (8.12%). Hence these altogether contribute more than ninety per cent towards total divergence. Therefore, these characters may be given importance during hybridization programme.

scholarly journals Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia

2018 ◽  
Vol 19 (6) ◽  
pp. 2374-2380
Author(s):  
INA ERLINAWATI ◽  
ABINAWANTO ABINAWANTO ◽  
ANDI SALAMAH ◽  
RUGAYAH RUGAYAH
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Nutritional Value ◽  
Island Population ◽  
Diversity Analysis ◽  
Mean Values ◽  
Genetic Diversity Analysis ◽  
North Sulawesi ◽  
Polymorphic Bands ◽  
First Time

Erlinawati I, Abinawanto, Salamah A, Rugayah. 2018. Genetic diversity analysis of daluga (Cyrtosperma merkusii) using Sequence-Related Amplified Polymorphism in Siau, Sangihe and Talaud Islands, North Sulawesi, Indonesia. Biodiversitas 19: 2374-2380. Daluga or the giant swamp taro (Cyrtosperma merkusii (Hassk.) Schott, a member of Araceae, is one of the minor tuber crops in Indonesia. It has high nutritional value for alternative food. Sequences-Related Amplified Polymorphism (SRAP) primers combinations were used for the first time to assess the genetic diversity among 36 of daluga accessions from several locations in Siau, Sangihe and Talaud islands, North Sulawesi, Indonesia. All of the samples were successfully extracted from silica-gel dried leaves using CTAB methods with minor modifications. Using six SRAP primer combinations, a total of 80 DNA fragments were yielded, varied from 75-1500 bp. The scoring of the fragments resulted in 68 (85.03%) polymorphic bands. Amongst four populations studied, Siau Island population has the highest level of genetic variation with mean values of Na = 1.9375±0.2436, Ne = 1.6409 ± 0.3255, PPL = 93.75%, H = 0.3614 ± 0.1516 and I = 0.5299 ± 0.1978. Meanwhile, Salibabu, Talaud Island population has the lowest level of genetic variation with mean values of Na = 1.5125 ± 0.5030, Ne = 1.3075 ± 0.3723 , PPL = 51.25%, H = 0.1800 ± 0.1989 and I = 0.2700 ± 0.2855. The high of polymorphic bands were generated by SRAP markers suitable for further analysis method in applied and basic of the genetic study on Cyrtosperma species and also related genera. This new genetic information can be used for baseline data for further research especially to improving the variation of daluga and for the conservation of daluga in the future.

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