Genetic Divergence (D2) Evaluation in Horsegram [Macrotyloma uniflorum (L) Verdcout] Genotypes

The experiment was performed at Research cum Instructional Farm, Shaheed Gundadhoor College of Agriculture and Research Station, Kumhrawand, Jagdalpur, Chhattisgarh. A total of 56germplasm lines and 1 check variety of horsegram were assessed for 10 quantitative traits during kharif2018.Genotypes were grouped into eight clusters. Clusters VI had maximum intra cluster distance while inter cluster distance was maximum between cluster VI and cluster VIII. Cluster III and cluster VIII had highest mean values for seed yield per plant characters. The characters viz., pods per plant followed by days to maturity and seed yield per plant were main contribution to total divergence. Based on the result genotypes belonging to clusters II, III and VIII are recommended for hybridization, as these genotypes showed good performance for seed yield and belong to the diverse clusters.

Morphological Characterization, Trait Variability and their Association, and Diversity Analysis Among Yard Long Bean (Vigna unguiculata (L.) Walp. subsp. Sesquipedalis (L.) Verdc.) Genotypes

Genetic variability has been greatly valued in yard-long bean, due to their narrow genetic base. To diversify the genetic base, yard long bean genotypes were evaluated to quantify the variation, to assess the extent of association between the yield complaint traits, diversity and for their utilization in crop improvement programmes. High phenotypic and genotypic coefficient of variations, heritability coupled with genetic advance over mean were observed most of the traits except internodal length, days to first and 50 per cent flowering, days to first and last picking, seeds per pod. Correlation and path analysis of variables namely pod length, pod width and pods per plant contributes high positive significant association with pod yield per plant, and maximum direct and indirect effect recorded for pods per plant, green pod length and green pod width. The genotypes were grouped into 12 clusters, the maximum divergence recorded between genotypes of Cluster XI and Cluster XII for traits pod yield per plant, vine length and yield per plot and pod length contributed high towards total divergence, the genetically diverse genotype of these clusters aids in the creation of larger recombinants, and would be an aid in the genetic improvement of yard-long bean genotypes.

Genetic Diversity in Fiber Related Traits of Tossa Jute (Corchorus Olitorius L.) Germplam

Genetic diversity of twenty-one genotypes of tossa jute was studied through Mahalanobis D2 statistic and principal component analysis for 15 quantitative traits related to yield at the research farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. The genotypes were grouped into four clusters. Cluster III contained the highest number of genotypes (nine), while cluster II had the lowest number of genotypes (three). The inter-cluster distances were higher than intra-cluster distances indicating wider genetic diversity among the genotypes of different clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the genotypes within the cluster. The highest intra-cluster distance was noticed for the cluster I and the lowest for cluster IV. The genotypes from cluster I could be selected as parents for hybridization for producing transgressive segregants. The highest inter-cluster distance was found between clusters I and IV followed by clusters I and III and the lowest was between clusters III and IV. Cluster IV recorded the highest mean value for plant height, base diameter, root length, leaf area, fresh weight with and without leaves, dry fiber weight and dry stick weight. In the total divergence, the highest contribution was recorded in plant height (65.1%) followed by base diameter (16.6%). The first two principal components, whose eigen values were greater than one, accounted for 81.7% of the total variations among the genotypes for fifteen fiber related traits. Considering the magnitude of cluster distance, cluster means for different characters and contribution of characters towards divergence, the genotypes G17, G18 from cluster IV; G1, G2, G5, G10, G16 from cluster I and G4 from cluster III could be selected as promising parents for hybridization program. SAARC J. Agri., 18(2): 27-37 (2020)

Estimation of Genetic Diversity in Rice (Oryza sativa L.) CMS Lines for High out Crossing Potential and Quality Traits

In the present study genetic diversity among 40 rice genotypes was evaluated using Mahalanobis D2 statistic based on morphological markers. Based on cluster analysis, the germplasms were grouped into 4 clusters of which Cluster I comprising of 23 genotypes was the largest group followed by the cluster II comprising of 15 genotypes, the clusters III and IV were represented by single genotype indicating high degree of heterogeneity among the genotypes. Maximum intra cluster distance observed in cluster II is (146.8) followed by cluster I (112.9) indicating the existence of variability among the genotypes within these clusters. The highest inter-cluster distance was observed between cluster IV and III (606.5), While the lowest was noticed between cluster II and III (222.9), describing that the genotypes included in these clusters were closely related. The kernel length, kernel length after cooking, kernel breadth, gel consistency, days to 50% flowering and plant height together contributed 90.1% towards total divergence. Therefore, these characters should be given importance during hybridization and selection of segregating populations. The genotypes falling in different clusters with the high mean for grain yield and other component characters can be utilized for the hybridization programme to obtain elite segregants.

Genetic Diversity Analysis for Yield Parameters in Rice (Oryza sativa. L) Genotypes

The present investigation was conducted during Kharif, 2019 at Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad. Twenty rice genotypes were studied to assess genetic diversity analysis for days to fifty percent flowering, plant height (cm), ear bearing tillers per m2, panicle length (cm), number of filled grains per panicle, test weight (g) and plot yield (kg/ha). Based on the analysis, the genotypes were grouped into four clusters. The maximum number of genotypes (14 genotypes) was grouped in cluster I. Cluster II and III consists of three and two genotypes respectively. Remaining cluster was represented by a single genotype. Maximum inter cluster distance was observed between I and II (27.46) followed by between cluster I and IV (23.19) and between cluster II and IV (20.43) indicating wider genetic diversity between genotypes. Hence these lines may be utilized in a further breeding programme for the exploitation of hybrid vigour. Among the seven traits studied, the number of filled grains per panicle contributed maximum divergence (42.68%) followed by days to 50% flowering (32.04%), grain yield per plot (12.01%) and ear bearing tillers per m2 (8.12%). Hence these altogether contribute more than ninety per cent towards total divergence. Therefore, these characters may be given importance during hybridization programme.

Phenotypic Diversity and Molecular diversity of Finger Millet Composite Collection and Identification of Trait Specific Germplasm for Use in Crop Improvement

A composite collection of finger millet consisting of 1000 accessions representing the diversity of the entire germplasm at ICRISAT gene bank was developed, including 622 accessions of ICRISAT core collection. Phenotyping of the composite collection for 15 quantitative traits and 20 SSR markers genotyping data resulted in the identification of promising traitspecific accessions. Principal component analysis with seven components indicated relative importance of the traits (days to 50 % flowering, plant height, peduncle length, ear head length, and panicle exertion) to total divergence. Clustering analysis grouped biological races into three clusters wherein cultivated races vulgaris, plana, elongata, and compacta were grouped in Cluster I and wild races spontanea in Cluster II and africana in Cluster III. Accessions were identified as useful for important traits such as early flowering (34), high grain yield (38), fodder yield (19); more fingers (29); basal tiller number (25) and ear head length (28).

Modern Drum Solos Over Ostinatos

Improvised drum solos by Steve Gadd, Vinnie Colaiuta, Dave Weckl, Trilok Gurtu, and Jojo Mayer are examined in the context of interactive frameworks comprising three distinct rhythmic elements: meter, ostinato, and drums. Meter and background ostinato provide complementary recurring cycles—one implicit, the other explicit—that the drummer uses as referents to create shifting contrapuntal configurations. These configurations can be understood in terms of degrees of synchrony between the three elements in the drums/meter/ostinato network, from full three-way convergence to total divergence and including intermediary states where one of the three elements is distinct from the other two. The chapter pays special attention to polymetric phrasing, an effective way for drummers to not only affirm their independence from meter and ostinato but also infuse the solo with playfully complex qualities.

Estimates of genetic variability, divergence, correlation and path coefficient for morphological traits in fenugreek (Trigonella foenum-graecum L.) genotypes

Seventeen genotypes of fenugreek (Trigonella foenum-graecum L.) were evaluated at ICAR-NRCSS, Ajmer (Rajasthan) during rabi 2014-15 and 2015-16. The highest GCV and PCV were observed for seed yield per plot followed by test weight and 5 plants seed yield. The highest genetic advance was observed for seed yield per plot followed by 5 plants seed yield and plant height. The highest heritability was estimated for 5 plants seed yield followed by plant height and number of primary branches. The genotypes were grouped into six clusters. Inter cluster distance was maximum between clusters IV and VI followed by III and VI while minimum between clusters II and IV. Whereas, the intra-cluster distance was maximum for Cluster-I. Among the eight characters studied for genetic divergence, 5 plants’ seed yield contributed the maximum accounting for 46.32% of total divergence, followed by number of primary branches (16.9%) and plant height (12.5%). It was concluded that improvement of seed yield in fenugreek can possible through selection for number of pod per plant, number of seeds per pod, plant height and number of primary branches. Molecular studies also supported the same findings.

Genetic diversity analysis and identification of promising lines for hybridization in Chickpea (Cicer arietinum L.)

Forty genotypes of chickpea were evaluated for assessing genetic divergence for different quantitative characters for improving yield potential of chickpea by using Mahalanobis D2 statistics. The genotypes were grouped into seven clusters suggesting considerable amount of genetic diversity in the material. The intra-cluster D2 value ranged from 0.00 to 9.06 while, intercluster D2 value ranged from 4.07 to 55.71. The maximum intra cluster distance was exhibited by cluster V followed by cluster II and cluster I. Cluster III had mono- genotype and emerged with contained highest cluster mean value grain yield per plot. Cluster II has highest mean value for number of pods per plant and lowest mean value for days to 50% flowering, days to maturity and plant height. Maximum contribution toward the total divergence was exhibited by 100-seed weight followed by number of pods per plant and minimum by days to maturity. Cluster IV and VI showing maximum inter cluster distance and crossing of genotypes Sabour chana-1 (BRC-1), GCP 105, PBC 503, DC 16-2, NBeG 776, RVSSG 42, G2016-43, GL 14042, IPC 2013-21, GNG 2338, GNG 2375 and KWR 108 from cluster VI suggested for improving grain yield per plot, 100-seed weight and earliness in flowering and ultimately enhancing the yield and chances of getting better recombinants in segregating generations.

Genetic divergence in Yam bean (Pachyrhizus erosus L. Urban) Germplasm

Altogether, 30 germplasm of Yam bean including two released varieties viz., Rajendra Misrikand-1(RM-1) and Rajendra Misrikand-2 (RM-2) were analyzed for genetic divergence among twelve studied characters. Analysis of variance revealed highly significant differences among the genotypes for all the twelve characters under study. The (PCV) was greater than genotypic coefficient of variance (GCV) for all the characters and high magnitude of PCV and GCV were observed for traits like tuber weight followed by tuber yield indicating that these traits could be used for tuber yield improvement but wide difference between GCV and PCV were recorded for most of the traits except for the tuber yield and tuber weight. All the thirty genotypes were grouped into 6 clusters using D2 statistics. Highest inter cluster distance was observed between cluster I and III (107.53) followed by cluster III and VI (97.61) and cluster I and II (78.54) on the basis of their genetic distances. Tuber yield followed by tuber weight and seed yield were having maximum contribution towards total divergence.