scholarly journals Possible influence of Loxoprofen in lipopolysaccharide induced alterations in immobility-time in tail suspension test in mice

2021 ◽  
Vol 7 (1) ◽  
pp. 22-27
Author(s):  
Kundu Smita S ◽  
Digvijaysinh G. Rana
Keyword(s):  
Tail Suspension Test ◽  
Tail Suspension ◽  
Immobility Time ◽  
Suspension Test

scholarly journals Evaluation of antidepressant activity of tramadol in comparison with imipramine in Swiss albino mice

2017 ◽  
Vol 6 (3) ◽  
pp. 695
Author(s):  
Chiranjeevi Bonda ◽  
Sudhir Pawar ◽  
Jaisen Lokhande
Keyword(s):  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Antidepressant Effect ◽  
Tail Suspension ◽  
Swim Test ◽  
Forced Swim ◽  
Group I ◽  
Immobility Time ◽  
Suspension Test

Background: The aim of the study was to evaluate the antidepressant effect of opioid analgesic tramadol using forced swim test and tail suspension test models.Methods: The antidepressant effect was assessed by recording the immobility time in Forced swim test (FST) and Tail suspension test (TST). The mice were randomly divided into five groups. Mice belonging to group I was given normal saline (0.1ml/kg) which acted as control. Group II received imipramine (15mg/kg) considered as the standard drug tramadol was given in graded dose (10, 20 and 40 mg/kg) to mice of groups III, IV, V respectively. All drugs were administered intraperitoneally for seven successive days; test was done on 7th day.Results: Tramadol and Imipramine showed antidepressant activity when compared to control. There is dose dependent increase in antidepressant activity of tramadol. The antidepressant activity of imipramine was significantly (P<0.05) more than tramadol at dose 10 and 20 mg/kg but antidepressant activity with tramadol 40mg/kg was comparable to imipramine treated mice.Conclusions: The results of this study indicated the presence of antidepressant activity of tramadol at 40mg/kg.

Reduced immobility time in the tail suspension test (TST) by chronic immobilization stress. Role of corticosterone and brain serotonergic and adrenergic receptors

1992 ◽  
Vol 7 (5) ◽  
pp. 235-238
Author(s):  
E De Castro-e-Silva ◽  
MF Peres ◽  
P Brito ◽  
C Cobas ◽  
A Saraiva ◽  
...  
Keyword(s):  
Immobilization Stress ◽  
Chronic Administration ◽  
Tail Suspension Test ◽  
Tail Suspension ◽  
Immobility Time ◽  
Adrenoceptor Blocker ◽  
Induced Changes ◽  
Chronic Immobilization Stress ◽  
Suspension Test

SummaryChronically stressed adult male Balb C mice were submitted to the tail suspension test. Chronic immobilization stress (6 h/d for 14 consecutive days) induced a significant reduction in immobility time when compared to non-stressed controls. Pretreatment with LY 53857, a serotonin 5HT2 antagonist, and IPS 339, a selective beta-2 adrenoceptor blocker, reversed immobility time to the levels of non-stressed controls. Chronic administration of corticosterone (100 mg/kg for 7 d) did not modify immobility time as compared to saline treated controls. It is suggested that both serotonergic and adrenergic pathways in the brain may participate in the stress-induced changes occurring in the tail suspension test response and that corticosterone does not appear to play a role in this process.

scholarly journals Homeostasis Imbalance of Microglia and Astrocytes Leads to Alteration in the Metabolites of the Kynurenine Pathway in LPS-Induced Depressive-Like Mice

2020 ◽  
Vol 21 (4) ◽  
pp. 1460 ◽  
Author(s):  
Xue Tao ◽  
Mingzhu Yan ◽  
Lisha Wang ◽  
Yunfeng Zhou ◽  
Zhi Wang ◽  
...  
Keyword(s):  
Tail Suspension Test ◽  
Treated Group ◽  
Dose Effect ◽  
Tail Suspension ◽  
Linear Dose ◽  
Immobility Time ◽  
Non Linear ◽  
Dose Dependent ◽  
The Brain ◽  
Suspension Test

In the pathology-oriented study of depression, inflammation hypothesis has received increasing attention for recent years. To mimic the depressive state caused by inflammation, rodents injected intraperitoneally with lipopolysaccharide (LPS) are usually used to stimulate an immune response. However, the dose of LPS that causes depressive-like behavior varies widely across many literatures. Previous study has uncovered the non-linearity in the dose-effect relationship for the depressive-like behavior induced by LPS administration, while the reason for this is still unclear. The present study aims to investigate the underlying mechanisms of this non-linear dose-dependent relationship. Four groups of mice were injected intraperitoneally with different doses of LPS (0, 0.32, 0.8, and 2 mg/kg). The tail suspension test was conducted to evaluate the depressive-like behavior within 23–25 h after the LPS administration. The neuroplasticity was assessed by the levels of related proteins, TrkB and PSD-95, and by the quantification of neurons using Nissl staining. The levels of the two metabolites of the kynurenine (KYN) pathway, 3-hydroxykynurenine (3-HK) and kynurenic acid (KYNA), in the brain were analyzed by LC-MS/MS. Activation of microglia and astrocytes in the brain were also determined by immunohistochemistry and western blotting, respectively. The results showed that, compared with the control group, the mice in the 0.8 mg/kg LPS-treated group exhibited a remarkable increase of immobility time in the tail suspension test. The neuroplasticity of mice in the 0.8 mg/kg LPS-treated group was also significantly reduced. The neurotoxic metabolite, 3-HK, was accumulated significantly in the hippocampus of the 0.8 mg/kg LPS-treated mice. Surprisingly, the 2 mg/kg LPS-treated mice did not exhibit a remarkable change of 3-HK but expressed increased KYNA significantly, which is neuroprotective. Furthermore, the activation of microglia and astrocytes, which were recognized as the primary source of 3-HK and KYNA, respectively, corresponded to the content of these two metabolites of the KYN pathway in each group. Consequently, it was speculated that the homeostasis of different glial cells could lead to a non-linear dose-dependent behavior by regulating the KYN pathway in the LPS-induced depressive-like mice.

scholarly journals Antidepressant-like Effect and Mechanisms of Essential Oils From Citrus Reticulata in Reserpine-induced Depression Model Mice

2021 ◽  
Author(s):  
Minghui Tang ◽  
Yong Ai ◽  
Siyang Zhu ◽  
Ni Song ◽  
Xian Xu ◽  
...  
Keyword(s):  
Essential Oil ◽  
Forced Swimming Test ◽  
Tail Suspension Test ◽  
Control Group ◽  
Citrus Reticulata ◽  
Tail Suspension ◽  
Immobility Time ◽  
Swimming Test ◽  
The Brain ◽  
Suspension Test

Abstract Citrus reticulata, has been used for various diseases such as cough. According to previous studies, the essential oil of C. reticulata (CREOs) have been shown to be effectively alleviate depression-like behaviors in mice. This study is aimed to investigate the antidepressant-like effect of CREOs in the rapid reserpine-induced depression model mice as well as its possible mechanisms. The experiment was conducted in six groups, each with four mice. The essential oil group and the control group were administered by sniffing (1h/d), while the reserpine group and fluoxetine group by intraperitoneal injection. Body weight, forced swimming test (FST) and tail suspension test (TST) were used to assess depressive behavior. The compositions and contents of CREOs were analyzed by GC-MS. The results indicated that reserpine could reduce the weight of mice and prolong the immobility time of FST and TST. Moreover, the level of 5HT-1A, GR and Nissl bodies in the brain tissue were significantly reduced, while the level of BDNF was increased in reserpine-treated mice. The administration of CREOs could effectively inhibit the weight loss and the prolongation of immobility time caused by reserpine. In addition, the treatment of CREOs has also been shown to reverse the changes in Nissl body, 5-HT, GR and BDNF levels. Limonene was the main active component of CREOs and might be related to the reduction of BDNF. By up-regulating the level of BDNF, CREOs could regulate the hyperexcitability of the HPA axis, thereby increasing the level of neurotransmitters and restoring neurons.

scholarly journals Gender, Depression and Zincum metallicum

2021 ◽  
Vol 15 (4) ◽  
pp. 4-4
Author(s):  
Silvio Leite Monteiro da Silva ◽  
Maria Martha Bernardi ◽  
Leoni Villano Bonamin
Keyword(s):  
International Law ◽  
Tail Suspension Test ◽  
P Value ◽  
Tail Suspension ◽  
Depression Prevalence ◽  
Immobility Time ◽  
Pregnant Mice ◽  
Gender Influences ◽  
Laboratory Models ◽  
Suspension Test

Background: Depression is a hot topic for research including for the homeopathy community. Laboratory models for human diseases offer the possibility to evaluate depression-like behavior in mice by the tail suspension test (TST), such as the challenge of mothers with induced inflammation by lipopolysaccharide (LPS) during gestation. Moreover, it is known that gender influences depression prevalence and treatment evolution as well in a homeopathic approach. Aims: Verify if the treatment of LPS-challenged pregnant mice with homeopathic Zincum metallicum would change the depression-like behavior of the offspring according to the gender. Methodology: the procedures with animals were previously approved according to local and international law (CEUA-UNIP protocol 156-13). Pregnant randomized BALB/c mice were treated in blind with Zincum metallicum 200c, 30c, 5c and Lactose 5c as control. The treatment lasted 31 days: 21 days from the mating day to the delivery plus ten days of lactating. At the 9.5 days of pregnancy, mothers were challenged or not with 100 microgram/Kg of LPS IP. The pups were separated by sex and mother treatment. The tail suspension test was performed to all pups after they grow up to adulthood (2 months old). Results: The treatment influenced the TST according to gender, but not according to mother’s LPS exposition. Female mice born from mothers treated with 200c potency showed reduction in the immobility time in relation to the control (p-value

scholarly journals Comparison of Noradrenergic and Serotonergic Antidepressants in Reducing Immobility Time in the Tail Suspension Test

2001 ◽  
Vol 85 (3) ◽  
pp. 327-330 ◽  
Author(s):  
Jun Fujishiro ◽  
Taiichiro Imanishi ◽  
Jun Baba ◽  
Kenji Kosaka
Keyword(s):  
Tail Suspension Test ◽  
Tail Suspension ◽  
Immobility Time ◽  
Suspension Test

scholarly journals Methanol Stem Bark Extract of Tamarindus indica Exhibited Antidepressant Activity in Mice

2021 ◽  
Vol 16 (2) ◽  
pp. 39-43
Author(s):  
S. Yunusa ◽  
I.D. Bidazun ◽  
S.Y. Magaji ◽  
F.H. Ashemi
Keyword(s):  
Lethal Dose ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Stem Bark ◽  
Bark Extract ◽  
Tamarindus Indica ◽  
Tail Suspension ◽  
Immobility Time ◽  
Stem Bark Extract ◽  
Suspension Test

Background: Depression is one of the most common psychiatric disorders affecting nearly 17% of the world population and the existing antidepressant drugs used in clinical settings are largely associated with serious side effects. Tamarindus indica (Fabaceae) is a plant that has been used ethno-medicinally as a remedy for depression.Objectives: The objective of this study was to determine the antidepressant activity of methanol stem bark extract of Tamarindus indica using mice, also to determine the LD50 and phytoconstituents of same extract.Methods: Fresh leaves of Tamarindus indica were collected, pulverised and extracted using 70% v/v methanol. Phyto-constituents of the extract were screened and Median lethal dose (LD50) was determined using standard protocols. Antidepressant effects of the extract were investigated using Tail suspension test (TST) and forced swimming test (FST) models.Results: The results of median lethal dose revealed the LD50 value of 470 mg/kg body weight and the phytochemical screening showed the presence of saponins, alkaloids and glycosides. The extract at the doses of 75 and 150 mg/kg body weight significantly (p<0.05) and dose-dependently decreased the immobility time in tail suspension test (TST) as compared to control. In forced swim test, the extract significantly (p<0.05) decreased the immobility time only at the highest tested dose (150 mg/kg) when compared to placebo group.Conclusion: Methanol stem bark extract of Tamarindus indica contains secondary metabolites that possess antidepressant action and thus, justifying the traditional use of this plant in the treatment of mental disorders including depression.

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