ROSMARINIC ACID CONTENT OF GENETICALLY STABLE CLONES OF Ocimum americanum L. (LEMON BASIL) REGENERATED THROUGH IN VITRO SHOOT BUD MULTIPLICATION AND ROOT CULTURE

In vitro root cultures provide an alternative means for producing secondary metabolites of pharmaceutical and industrial importance. The present investigation is an attempt to study the effect of methyl jasmonate (MeJA) as an elicitor, on the production of rosmarinic acid through in vitro root cultures of Ocimum basilicum L. The rosmarinic acid is a preformed constitutively accumulated defense compound having several biological activities including antibacterial, antiviral, and antioxidative activity. Uniform progeny of O. basilicum L. was obtained through in vitro shoot bud multiplication as reliable source of explants. In vitro root cultures were established in one fourth strength of Murashige and Skoog (MS) liquid medium supplemented with 1.0 mg l–1 ?-napthaleneacetic acid (NAA). Methyl jasmonate, was supplemented to the root cultures at different concentrations (0.01, 0.1 and 1.0 mM), and their effect on total phenol content as well as rosmarinic acid production was studied. Rosmarinic acid was quantified by high performance liquid chromatography (HPLC). Compared to the control set of cultures 1.60 fold increase in rosmarinic acid concentration (4.05 ± 0.74 % of dry wt) was observed in elicitor treated cultures.

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Effects of Light Quality and Chemical Elicitors on the Growth Parameters and Rosmarinic Acid Content of in vitro Cultures of Hyptis pectinata (L.) Poit.

Journal of the Brazilian Chemical Society ◽

10.21577/0103-5053.20190159 ◽

2019 ◽

Author(s):

Rita Pedroso ◽

Leticia Pimenta ◽

Marcos Tozatti ◽

Núbia Branquinho ◽

Alessandra Hara ◽

...

Keyword(s):

Acid Content ◽

Rosmarinic Acid ◽

Light Quality ◽

Growth Parameters ◽

In Vitro Cultures

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Effect of cytokinins on in vitro multiplication, volatiles composition and rosmarinic acid content of Thymus leucotrichus Hal. shoots

3 Biotech ◽

10.1007/s13205-018-1206-2 ◽

2018 ◽

Vol 8 (3) ◽

Cited By ~ 11

Author(s):

Tuba Bekircan ◽

Ahmet Yaşar ◽

Sercan Yıldırım ◽

Münevver Sökmen ◽

Atalay Sökmen

Keyword(s):

Acid Content ◽

Rosmarinic Acid ◽

In Vitro Multiplication ◽

Volatiles Composition

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Comparative study of rosmarinic acid content in Satureja species

Planta Medica ◽

10.1055/s-0030-1264689 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

H Hajimehdipoor ◽

M Shekarchi ◽

S Saeidnia ◽

A Gohari ◽

Z Abedi

Keyword(s):

Comparative Study ◽

Acid Content ◽

Rosmarinic Acid

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Rosmarinic acid content in antidiabetic aqueous extract of Ocimum canum Sims grown in Ghana

Planta Medica ◽

10.1055/s-0033-1352208 ◽

2013 ◽

Vol 79 (13) ◽

Author(s):

AO Affum ◽

MA Berhow ◽

BA Gyan

Keyword(s):

Aqueous Extract ◽

Acid Content ◽

Rosmarinic Acid

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Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽

10.3390/ani11010108 ◽

2021 ◽

Vol 11 (1) ◽

pp. 108

Author(s):

Yichong Wang ◽

Sijiong Yu ◽

Yang Li ◽

Shuang Zhang ◽

Xiaolong Qi ◽

...

Keyword(s):

Fatty Acids ◽

Acetic Acid ◽

Propionic Acid ◽

Acid Content ◽

Volatile Fatty Acids ◽

Rumen Fermentation ◽

Gas Production ◽

Quadratic Response ◽

In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

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Ocimum campechianum Mill. from Amazonian Ecuador: Chemical Composition and Biological Activities of Extracts and Their Main Constituents (Eugenol and Rosmarinic Acid)

Molecules ◽

10.3390/molecules26010084 ◽

2020 ◽

Vol 26 (1) ◽

pp. 84

Author(s):

Massimo Tacchini ◽

Monica Paulina Echeverria Guevara ◽

Alessandro Grandini ◽

Immacolata Maresca ◽

Matteo Radice ◽

...

Keyword(s):

Mass Spectrometry ◽

Pseudomonas Syringae ◽

Rosmarinic Acid ◽

Plant Protection ◽

Biological Activities ◽

Radical Scavenger ◽

Radical Scavenger Activity ◽

Antioxidant Agents ◽

Scavenger Activity

The essential oil (EO), the methanolic (MeOH), and the 70% ethanolic (70% EtOH) extracts obtained from the aerial parts of Ocimum campechianum Mill. (Ecuador) were chemically characterized through gas-chromatography coupled to mass spectrometry detector (GC-MS), high-performance liquid chromatography coupled to diode array-mass spectrometry detectors (HPLC-DAD-MS) and studied for their in vitro biological activity. The radical scavenger activity, performed by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, highlighted significant IC50 values for the EO, extracts and their main constituents (eugenol and rosmarinic acid). EO (and eugenol) showed noteworthy activity against Pseudomonas syringae pv. syringae and a moderate effect against clinical Candida strains, with possible synergism in association to fluconazole against the latter microorganisms. The extracts and pure molecules exhibited weak cytotoxic activity against the HaCat cell line and no mutagenicity against Salmonella typhimurium TA98 and TA100 strains, giving indication of safety. Instead, EO showed a weak activity against adenocarcinomic human alveolar basal epithelial cells (A549). The above-mentioned evidence leads us to suggest a potential use of the crude drug, extracts, and EO in cosmetic formulation and food supplements as antioxidant agents. In addition, EO may also have a possible application in plant protection and anti-Candida formulations.

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The Effect of Carnosol, Carnosic Acid and Rosmarinic Acid on the Oxidative Stability of Fat-Filled Milk Powders throughout Accelerated Oxidation Storage

Antioxidants ◽

10.3390/antiox10050762 ◽

2021 ◽

Vol 10 (5) ◽

pp. 762

Author(s):

Katerina Tzima ◽

Nigel P. Brunton ◽

Noel A. McCarthy ◽

Kieran N. Kilcawley ◽

David T. Mannion ◽

...

Keyword(s):

Rosmarinic Acid ◽

Solid Phase ◽

Thiobarbituric Acid ◽

Butylated Hydroxytoluene ◽

Carnosic Acid ◽

Gas Chromatography Mass Spectrometry ◽

Peroxide Values ◽

Antioxidant Potency ◽

Antioxidant Effects

The in vitro antioxidant effects of the most potent antioxidants of rosemary, namely carnosol, carnosic acid and rosmarinic acid (c: ca: ra) were assessed in fat-filled milk powders (FFMPs) under accelerated conditions (40 °C and relative humidity (RH) 23%) over 90 days. Lipid oxidation was assessed in FFMPs by measuring peroxide values (PVs), thiobarbituric acid reactive substances (TBARS) and aroma volatiles using headspace (HS) solid-phase microextraction (SPME) coupled to gas-chromatography-mass spectrometry (GC-MS). The antioxidant potency of c: ca: ra exhibited a concentration-related effect (308 ppm > 200 ppm > 77 ppm), with the highest concentration being the most effective at controlling the formation of TBARS and PVs. At a concentration of 308 ppm c: ca: ra were particularly effective (p < 0.05) in inhibiting all the evaluated oxidation indices (primary and secondary) compared to the control samples, but in some cases less effectively (p < 0.05) than butylated hydroxyanisole: butylated hydroxytoluene (BHA: BHT) (200 ppm).

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