scholarly journals Conditioned Medium from the Stem Cells of Human Exfoliated Deciduous Teeth Rescues White Matter Injury in Neonatal Rats

2021 ◽  
Vol 4 (8) ◽  
pp. 06-13
Author(s):  
Lingyi Huang ◽  
Zizhuo Zheng ◽  
Hui Li ◽  
Yi Qu ◽  
Fengyan Zhao
Keyword(s):  
Stem Cells ◽  
White Matter ◽  
Conditioned Medium ◽  
White Matter Injury ◽  
Deciduous Teeth ◽  
Neonatal Rats ◽  
Human Exfoliated Deciduous Teeth

A Novel Treatment with Stem Cells from Human Exfoliated Deciduous Teeth for Hypoxic-Ischemic Encephalopathy in Neonatal Rats

2020 ◽  
Vol 29 (2) ◽  
pp. 63-74
Author(s):  
Yuma Kitase ◽  
Yoshiaki Sato ◽  
Kazuto Ueda ◽  
Toshihiko Suzuki ◽  
Alkisti Mikrogeorgiou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hypoxic Ischemic Encephalopathy ◽  
Deciduous Teeth ◽  
Neonatal Rats ◽  
Novel Treatment ◽  
Human Exfoliated Deciduous Teeth ◽  
Ischemic Encephalopathy

scholarly journals Conditioned medium from stem cells derived from human exfoliated deciduous teeth ameliorates NASH via the Gut-Liver axis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hisanori Muto ◽  
Takanori Ito ◽  
Taku Tanaka ◽  
Shinya Yokoyama ◽  
Kenta Yamamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Conditioned Medium ◽  
Parenchymal Cell ◽  
Deciduous Teeth ◽  
Intestinal Barrier Function ◽  
Nash Model ◽  
Alcoholic Steatohepatitis ◽  
Tnf Α ◽  
Human Exfoliated Deciduous Teeth

AbstractNon-alcoholic steatohepatitis (NASH) occurrence has been increasing and is becoming a major cause of liver cirrhosis and liver cancer. However, effective treatments for NASH are still lacking. We examined the benefits of serum-free conditioned medium from stem cells derived from human exfoliated deciduous teeth (SHED-CM) on a murine non-alcoholic steatohepatitis (NASH) model induced by a combination of Western diet (WD) and repeated administration of low doses of carbon tetrachloride intraperitoneally, focusing on the gut-liver axis. We showed that repeated intravenous administration of SHED-CM significantly ameliorated histological liver fibrosis and inflammation in a murine NASH model. SHED-CM inhibited parenchymal cell apoptosis and reduced the activation of inflammatory macrophages. Gene expression of pro-inflammatory and pro-fibrotic mediators (such as Tnf-α, Tgf-β, and Ccl-2) in the liver was reduced in mice treated with SHED-CM. Furthermore, SHED-CM protected intestinal tight junctions and maintained intestinal barrier function, while suppressing gene expression of the receptor for endotoxin, Toll-like receptor 4, in the liver. SHED-CM promoted the recovery of Caco-2 monolayer dysfunction induced by IFN-γ and TNF-α in vitro. Our findings suggest that SHED-CM may inhibit NASH fibrosis via the gut-liver axis, in addition to its protective effect on hepatocytes and the induction of macrophages with unique anti-inflammatory phenotypes.

scholarly journals Protective effects of stem cells from human exfoliated deciduous teeth derived conditioned medium on osteoarthritic chondrocytes

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0238449 ◽  
Author(s):  
Suleiman Alhaji Muhammad ◽  
Norshariza Nordin ◽  
Paisal Hussin ◽  
Muhammad Zulfadli Mehat ◽  
Noor Hayaty Abu Kasim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Deciduous Teeth ◽  
Protective Effects ◽  
Osteoarthritic Chondrocytes ◽  
Human Exfoliated Deciduous Teeth

Stem Cells Derived from Human Exfoliated Deciduous teeth [shed] in Neuronal Disorders: A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Minu Anoop ◽  
Indrani Datta
Keyword(s):  
Stem Cells ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Therapeutic Potential ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Proliferative Potential ◽  
Pulp Tissue ◽  
Human Exfoliated Deciduous Teeth

: Most conventional treatments for neurodegenerative diseases fail due to their focus on neuroprotection rather than neurorestoration. Stem cell‐based therapies are becoming a potential treatment option for neurodegenerative diseases as they can home in, engraft, differentiate and produce factors for CNS recovery. Stem cells derived from human dental pulp tissue differ from other sources of mesenchymal stem cells due to their embryonic neural crest origin and neurotrophic property. These include both dental pulp stem cells [DPSCs] from dental pulp tissues of human permanent teeth and stem cells from human exfoliated deciduous teeth [SHED]. SHED offer many advantages over other types of MSCs such as good proliferative potential, minimal invasive procurement, neuronal differentiation and neurotrophic capacity, and negligible ethical concerns. The therapeutic potential of SHED is attributed to the paracrine action of extracellularly released secreted factors, specifically the secretome, of which exosomes is a key component. SHED and its conditioned media can be effective in neurodegeneration through multiple mechanisms, including cell replacement, paracrine effects, angiogenesis, synaptogenesis, immunomodulation, and apoptosis inhibition, and SHED exosomes offer an ideal refined bed-to-bench formulation in neurodegenerative disorders. However, in spite of these advantages, there are still some limitations of SHED exosome therapy, such as the effectiveness of long-term storage of SHED and their exosomes, the development of a robust GMP-grade manufacturing protocol, optimization of the route of administration, and evaluation of the efficacy and safety in humans. In this review, we have addressed the isolation, collection and properties of SHED along with its therapeutic potential on in vitro and in vivo neuronal disorder models as evident from the published literature.

scholarly journals Pioglitazone Ameliorates Lipopolysaccharide-Induced Behavioral Impairment, Brain Inflammation, White Matter Injury and Mitochondrial Dysfunction in Neonatal Rats

2021 ◽  
Vol 22 (12) ◽  
pp. 6306
Author(s):  
Jiann-Horng Yeh ◽  
Kuo-Ching Wang ◽  
Asuka Kaizaki ◽  
Jonathan W. Lee ◽  
Han-Chi Wei ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
White Matter ◽  
Brain Inflammation ◽  
White Matter Injury ◽  
Neonatal Rat ◽  
Mitochondrial Activity ◽  
Neonatal Rats ◽  
Activated Microglia ◽  
Brain White Matter ◽  
Performance Reduction

Previous studies have demonstrated that pioglitazone, a peroxisome proliferator-activated receptor gamma (PPARγ) agonist, inhibits ischemia-induced brain injury. The present study was conducted to examine whether pioglitazone can reduce impairment of behavioral deficits mediated by inflammatory-induced brain white matter injury in neonatal rats. Intraperitoneal (i.p.) injection of lipopolysaccharide (LPS, 2 mg/kg) was administered to Sprague–Dawley rat pups on postnatal day 5 (P5), and i.p. administration of pioglitazone (20 mg/kg) or vehicle was performed 5 min after LPS injection. Sensorimotor behavioral tests were performed 24 h after LPS exposure, and changes in biochemistry of the brain was examined after these tests. The results show that systemic LPS exposure resulted in impaired sensorimotor behavioral performance, reduction of oligodendrocytes and mitochondrial activity, and increases in lipid peroxidation and brain inflammation, as indicated by the increment of interleukin-1β (IL-1β) levels and number of activated microglia in the neonatal rat brain. Pioglitazone treatment significantly improved LPS-induced neurobehavioral and physiological disturbances including the loss of body weight, hypothermia, righting reflex, wire-hanging maneuver, negative geotaxis, and hind-limb suspension in neonatal rats. The neuroprotective effect of pioglitazone against the loss of oligodendrocytes and mitochondrial activity was associated with attenuation of LPS-induced increment of thiobarbituric acid reactive substances (TBARS) content, IL-1β levels and number of activated microglia in neonatal rats. Our results show that pioglitazone prevents neurobehavioral disturbances induced by systemic LPS exposure in neonatal rats, and its neuroprotective effects are associated with its impact on microglial activation, IL-1β induction, lipid peroxidation, oligodendrocyte production and mitochondrial activity.

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