scholarly journals The significance of amniotic fluid immunological analysis for the prediction of intrauterine infection

2020 ◽  
Vol 47 (6) ◽  
pp. 810
Keyword(s):  
Amniotic Fluid ◽  
Intrauterine Infection ◽  
Immunological Analysis

New prognostic protein markers for intrauterine infections in preterm birth

2020 ◽  
Vol 19 (5) ◽  
pp. 29-35
Author(s):  
L.V. Renge ◽  
◽  
E.Yu. Grigoryeva ◽  
V.N. Zorina ◽  
A.E. Vlasenko ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Amniotic Fluid ◽  
Umbilical Cord ◽  
Biological Fluids ◽  
Intrauterine Infection ◽  
Maternal Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Markers ◽  
Cord Serum ◽  
Intrauterine Infections

Objective. To identify prognostic markers of intrauterine infection (IUI) of the fetus and newborn in maternal serum, amniotic fluid, and umbilical cord serum in case of preterm birth. Patients and methods. We examined 93 pregnant women who had preterm birth (PB) from 24 to 33 weeks of gestation. Thirtyfive women delivered babies without any signs of IUI, while 30 women had newborns with mild IUI (conjunctivitis, lymphadenitis, pyoderma) and 28 women had newborns with severe IUI (early neonatal sepsis, advanced herpesvirus infection, chlamydiosis, candidiasis, pneumonia, and meningitis). We measured the levels of alpha 2-macroglobulin (α2-MG) in maternal serum (MS), umbilical cord serum (UCS) using rocket immunoelectrophoresis and in amniotic fluid (AF) using enzyme-linked immunosorbent assay (ELISA). The level of lactoferrin (LF) was assessed using ELISA. MS and UCS levels of albumin (ALB) were measured using biochemical methods, while AF level ALB was evaluated using rocket immunoelectrophoresis. Statistical analysis was performed using logistic regression. Results. We found no association between the concentration of LF in all biological fluids and the condition of newborns. Levels of ALB in MS and UCS also demonstrated no correlation with the condition of newborns. AF ALB in women who delivered babies with IUI (any grade of severity) was significantly higher than that in women who delivered babies without IUI. Women who delivered babies with severe IUI demonstrated the lowest concentration of α2-MG in their serum, whereas women who delivered babies with mild IUI had the highest α2-MG concentration. The AF α2-MG level was 10 to 20 times higher in women who had babies with IUI (any grade of severity) compared to those who had babies without IUI. Conclusion. Low α2-MG level in MS (<2.2 g/L) along with elevated α2-MG level in AF (≥6.0 mg/L) in 86–89% of PB cases indicated generalized fetal IUI and required urgent delivery without prolongation of pregnancy. Key words: albumin, alpha-2-macroglobulin, intrauterine infection, lactoferrin. premature birth

scholarly journals Salmonella enterica serovar Stanley intrauterine infection in a stillborn calf - case report

2013 ◽  
Vol 82 (4) ◽  
pp. 363-367 ◽  
Author(s):  
Paulina Jawor ◽  
Tadeusz Stefaniak ◽  
Zenon Sołtysiak ◽  
Stanisław Dzimira ◽  
Michał Bednarski
Keyword(s):  
Case Report ◽  
Amniotic Fluid ◽  
Frontal Cortex ◽  
Salmonella Enterica ◽  
Bacterial Culture ◽  
Histopathological Examination ◽  
Intrauterine Infection ◽  
Inflammatory Infiltration ◽  
Neonatal Diarrhoea ◽  
The Brain

The aim of this study was to report the response to a bacterial intrauterine infection in a calf. A stillborn calf, dam’s blood and amniotic fluid were submitted for examination. Necropsy of the calf was performed and IgG1, IgG2, IgM, IL-6 in the calf’s serum, Il-6 in the dam’s serum, and amniotic fluid were estimated. During necropsy, fluid in pleural and peritoneal cavities stained with haemoglobin and diagonal fissures in the aortic arch endothelium were found.Salmonella entericaserovar Stanley was isolated from the spleen, lungs and abomasal fluid.Histopathological examination revealed: inflammatory infiltration and haemorrhages in lungs and small perivascular haemorrhages in the frontal cortex and near the lateral ventricles of the white matter, focal gliosis in the frontal cortex, and neuronal atrophy of the dentate gyrus with diffuse glial cells proliferation in the brain. The concentration of IgG1in the calf’s serum was increased and IL-6 was detected in both the dam’s blood and amniotic fluid. Necropsy, bacterial culture and immunological findings in the stillborn calf confirmed the intrauterine infectionwithSalmonellaStanley as the cause of death. Meanwhile, neonatal diarrhoea (incidence 46%) with high mortality (54%) occurred on the same farm. From diarrhoeic calves,SalmonellaTyphimurium andS.Enteritidis were isolated. Based on available literature this is the first evidence ofSalmonella entericaserovar Stanley isolation from a stillborn calf.

scholarly journals Maternal Intravenous Treatment with either Azithromycin or Solithromycin Clears Ureaplasma parvum from the Amniotic Fluid in an Ovine Model of Intrauterine Infection

2014 ◽  
Vol 58 (9) ◽  
pp. 5413-5420 ◽  
Author(s):  
Yuichiro Miura ◽  
Matthew S. Payne ◽  
Jeffrey A. Keelan ◽  
Andres Noe ◽  
Sean Carter ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Intrauterine Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sheep Model ◽  
Content Type ◽  
Ureaplasma Parvum ◽  
Treatment Regimens ◽  
Significant Difference ◽  
Adverse Neonatal Outcomes ◽  
Discernible Difference

ABSTRACTIntrauterine infection withUreaplasmaspp. is strongly associated with preterm birth and adverse neonatal outcomes. We assessed whether combined intraamniotic (IA) and maternal intravenous (IV) treatment with one of two candidate antibiotics, azithromycin (AZ) or solithromycin (SOLI), would eradicate intrauterineUreaplasma parvuminfection in a sheep model of pregnancy. Sheep with singleton pregnancies received an IA injection ofU. parvumserovar 3 at 85 days of gestational age (GA). At 120 days of GA, animals (n= 5 to 8/group) received one of the following treatments: (i) maternal IV SOLI with a single IA injection of vehicle (IV SOLI only); (ii) maternal IV SOLI with a single IA injection of SOLI (IV+IA SOLI); (iii) maternal IV AZ and a single IA injection of vehicle (IV AZ only); (iv) maternal IV AZ and a single IA injection of AZ (IV+IA AZ); or (v) maternal IV and single IA injection of vehicle (control). Lambs were surgically delivered at 125 days of GA. Treatment efficacies were assessed byU. parvumculture, quantitative PCR, enzyme-linked immunosorbent assay, and histopathology. Amniotic fluid (AF) from all control animals contained culturableU. parvum. AF, lung, and chorioamnion from all AZ- or SOLI-treated animals (IV only or IV plus IA) were negative for culturableU. parvum. Relative to the results for the control, the levels of expression of interleukin 1β (IL-1β), IL-6, IL-8, and monocyte chemoattractant protein 2 (MCP-2) in fetal skin were significantly decreased in the IV SOLI-only group, the MCP-1 protein concentration in the amniotic fluid was significantly increased in the IV+IA SOLI group, and there was no significant difference in the histological inflammation scoring of lung or chorioamnion among the five groups. In the present study, treatment with either AZ or SOLI (IV only or IV+IA) effectively eradicated macrolide-sensitiveU. parvumfrom the AF. There was no discernible difference in antibiotic therapy efficacy between IV-only and IV+IA treatment regimens relative to the results for the control.

scholarly journals Amniotic Fluid Defensins: Potential Markers of Subclinical Intrauterine Infection

1998 ◽  
Vol 27 (3) ◽  
pp. 513-518 ◽  
Author(s):  
R. P. Heine ◽  
H. Wiesenfeld ◽  
L. Mortimer ◽  
Greig
Keyword(s):  
Amniotic Fluid ◽  
Intrauterine Infection
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