High Frequency Evoked Action Potentials Recorded from the Ear Canal in Man

1976 ◽  
Vol 5 (3) ◽  
pp. 157-164 ◽  
Author(s):  
C. Elberling
Keyword(s):  
High Frequency ◽  
Action Potentials ◽  
Ear Canal

scholarly journals Cochlear compound action potentials from high-level tone bursts originate from wide cochlear regions that are offset toward the most sensitive cochlear region

2019 ◽  
Vol 121 (3) ◽  
pp. 1018-1033 ◽  
Author(s):  
C. Lee ◽  
J. J. Guinan ◽  
M. A. Rutherford ◽  
W. A. Kaf ◽  
K. M. Kennedy ◽  
...  
Keyword(s):  
High Frequency ◽  
Action Potentials ◽  
Tuning Curve ◽  
Low Frequency ◽  
Sound Level ◽  
Frequency Tone ◽  
Compound Action Potentials ◽  
High Level ◽  
Level Tone ◽  
Compound Action

Little is known about the spatial origins of auditory nerve (AN) compound action potentials (CAPs) evoked by moderate to intense sounds. We studied the spatial origins of AN CAPs evoked by 2- to 16-kHz tone bursts at several sound levels by slowly injecting kainic acid solution into the cochlear apex of anesthetized guinea pigs. As the solution flowed from apex to base, it sequentially reduced CAP responses from low- to high-frequency cochlear regions. The times at which CAPs were reduced, combined with the cochlear location traversed by the solution at that time, showed the cochlear origin of the removed CAP component. For low-level tone bursts, the CAP origin along the cochlea was centered at the characteristic frequency (CF). As sound level increased, the CAP center shifted basally for low-frequency tone bursts but apically for high-frequency tone bursts. The apical shift was surprising because it is opposite the shift expected from AN tuning curve and basilar membrane motion asymmetries. For almost all high-level tone bursts, CAP spatial origins extended over 2 octaves along the cochlea. Surprisingly, CAPs evoked by high-level low-frequency (including 2 kHz) tone bursts showed little CAP contribution from CF regions ≤ 2 kHz. Our results can be mostly explained by spectral splatter from the tone-burst rise times, excitation in AN tuning-curve “tails,” and asynchronous AN responses to high-level energy ≤ 2 kHz. This is the first time CAP origins have been identified by a spatially specific technique. Our results show the need for revising the interpretation of the cochlear origins of high-level CAPs-ABR wave 1. NEW & NOTEWORTHY Cochlear compound action potentials (CAPs) and auditory brain stem responses (ABRs) are routinely used in laboratories and clinics. They are typically interpreted as arising from the cochlear region tuned to the stimulus frequency. However, as sound level is increased, the cochlear origins of CAPs from tone bursts of all frequencies become very wide and their centers shift toward the most sensitive cochlear region. The standard interpretation of CAPs and ABRs from moderate to intense stimuli needs revision.

Spontaneous Subthreshold Membrane Potential Fluctuations and Action Potential Variability of Rat Corticostriatal and Striatal Neurons In Vivo

1997 ◽  
Vol 77 (4) ◽  
pp. 1697-1715 ◽  
Author(s):  
Edward A. Stern ◽  
Anthony E. Kincaid ◽  
Charles J. Wilson
Keyword(s):  
Membrane Potential ◽  
Synaptic Input ◽  
High Frequency ◽  
Action Potentials ◽  
State Transitions ◽  
Striatal Neurons ◽  
Interspike Intervals ◽  
Potential Fluctuations ◽  
Membrane Potential Fluctuations

Stern, Edward A., Anthony E. Kincaid, and Charles J. Wilson. Spontaneous subthreshold membrane potential fluctuations and action potential variability of rat corticostriatal and striatal neurons in vivo. J. Neurophysiol. 77: 1697–1715, 1997. We measured the timing of spontaneous membrane potential fluctuations and action potentials of medial and lateral agranular corticostriatal and striatal neurons with the use of in vivo intracellular recordings in urethan-anesthetized rats. All neurons showed spontaneous subthreshold membrane potential shifts from 7 to 32 mV in amplitude, fluctuating between a hyperpolarized down state and depolarized up state. Action potentials arose only during the up state. The membrane potential state transitions showed a weak periodicity with a peak frequency near 1 Hz. The peak of the frequency spectra was broad in all neurons, indicating that the membrane potential fluctuations were not dominated by a single periodic function. At frequencies >1 Hz, the log of magnitude decreased linearly with the log of frequency in all neurons. No serial dependence was found for up and down state durations, or for the time between successive up or down state transitions, showing that the up and down state transitions are not due to superimposition of noisy inputs onto a single frequency. Monte Carlo simulations of stochastic synaptic inputs to a uniform finite cylinder showed that the Fourier spectra obtained for corticostriatal and striatal neurons are inconsistent with a Poisson-like synaptic input, demonstrating that the up state is not due to an increase in the strength of an unpatterned synaptic input. Frequency components arising from state transitions were separated from those arising from the smaller membrane potential fluctuations within each state. A larger proportion of the total signal was represented by the fluctuations within states, especially in the up state, than was predicted by the simulations. The individual state spectra did not correspond to those of random synaptic inputs, but reproduced the spectra of the up and down state transitions. This suggests that the process causing the state transitions and the process responsible for synaptic input may be the same. A high-frequency periodic component in the up states was found in the majority of the corticostriatal cells in the sample. The average size of the component was not different between neurons injected with QX-314 and control neurons. The high-frequency component was not seen in any of our sample of striatal cells. Corticostriatal and striatal neurons' coefficients of variation of interspike intervals ranged from 1.0 to 1.9. When interspike intervals including a down state were subtracted from the calculation, the coefficient of variation ranged from 0.4 to 1.1, indicating that a substantial proportion of spike interval variance was due to the subthreshold membrane potential fluctuations.

scholarly journals Systems-based analysis of dendritic nonlinearities reveals temporal feature extraction in mouse L5 cortical neurons

2017 ◽  
Vol 117 (6) ◽  
pp. 2188-2208 ◽  
Author(s):  
Brian E. Kalmbach ◽  
Richard Gray ◽  
Daniel Johnston ◽  
Erik P. Cook
Keyword(s):  
High Frequency ◽  
Cortical Neurons ◽  
Action Potentials ◽  
Nonlinear Response ◽  
Functional Model ◽  
Low Frequency ◽  
Fast Variable ◽  
Nonlinear Component ◽  
Dendritic Spikes ◽  
Linear And Nonlinear

What do dendritic nonlinearities tell a neuron about signals injected into the dendrite? Linear and nonlinear dendritic components affect how time-varying inputs are transformed into action potentials (APs), but the relative contribution of each component is unclear. We developed a novel systems-identification approach to isolate the nonlinear response of layer 5 pyramidal neuron dendrites in mouse prefrontal cortex in response to dendritic current injections. We then quantified the nonlinear component and its effect on the soma, using functional models composed of linear filters and static nonlinearities. Both noise and waveform current injections revealed linear and nonlinear components in the dendritic response. The nonlinear component consisted of fast Na+ spikes that varied in amplitude 10-fold in a single neuron. A functional model reproduced the timing and amplitude of the dendritic spikes and revealed that they were selective to a preferred input dynamic (~4.5 ms rise time). The selectivity of the dendritic spikes became wider in the presence of additive noise, which was also predicted by the functional model. A second functional model revealed that the dendritic spikes were weakly boosted before being linearly integrated at the soma. For both our noise and waveform dendritic input, somatic APs were dependent on the somatic integration of the stimulus, followed a subset of large dendritic spikes, and were selective to the same input dynamics preferred by the dendrites. Our results suggest that the amplitude of fast dendritic spikes conveys information about high-frequency features in the dendritic input, which is then combined with low-frequency somatic integration. NEW & NOTEWORTHY The nonlinear response of layer 5 mouse pyramidal dendrites was isolated with a novel systems-based approach. In response to dendritic current injections, the nonlinear component contained mostly fast, variable-amplitude, Na+ spikes. A functional model accounted for the timing and amplitude of the dendritic spikes and revealed that dendritic spikes are selective to a preferred input dynamic, which was verified experimentally. Thus, fast dendritic nonlinearities behave as high-frequency feature detectors that influence somatic action potentials.

Evaluation of the Repeatability and Accuracy of the Wideband Real-Ear-to-Coupler Difference

2018 ◽  
Vol 29 (06) ◽  
pp. 520-532 ◽  
Author(s):  
Jonathan M. Vaisberg ◽  
Paula Folkeard ◽  
John Pumford ◽  
Philipp Narten ◽  
Susan Scollie
Keyword(s):  
Hearing Aids ◽  
High Frequency ◽  
Repeated Measures ◽  
Ear Canal ◽  
Repeated Measures Anova ◽  
Absolute Agreement ◽  
The Difference ◽  
Db Difference ◽  
Additional Clinical Benefit ◽  
Reported Data

AbstractThe real-ear-to-coupler difference (RECD) is an ANSI standardized method for estimating ear canal sound pressure level (SPL) thresholds and assisting in the prediction of real-ear aided responses. It measures the difference in dB between the SPL produced in the ear canal and the SPL produced in an HA-1 2-cc coupler by the same sound source. Recent evidence demonstrates that extended high-frequency bandwidth, beyond the hearing aid bandwidth typically measured, is capable of providing additional clinical benefit. The industry has, in turn, moved toward developing hearing aids and verification equipment capable of producing and measuring extended high-frequency audible output. As a result, a revised RECD procedure conducted using a smaller, 0.4-cc coupler, known as the wideband-RECD (wRECD), has been introduced to facilitate extended high-frequency coupler-based measurements up to 12.5 kHz.This study aimed to (1) compare test–retest repeatability between the RECD and wRECD and (2) measure absolute agreement between the RECD and wRECD when both are referenced to a common coupler.RECDs and wRECDs were measured bilaterally in adult ears by calculating the dB difference in SPL between the ear canal and coupler responses. Real-ear probe microphone measures were completed twice per ear per participant for both foam-tip and customized earmold couplings using the Audioscan Verifit 1 and Verifit 2 fitting systems, followed by measurements in the respective couplers.Twenty-one adults (mean age = 67 yr, range = 19–78) with typical aural anatomy (as determined by measures of impedance and otoscopy) participated in this study, leading to a sample size of 42 ears.Repeatability within RECD and wRECD was assessed for each coupling configuration using a repeated-measures analysis of variance (ANOVA) with test–retest and frequency as within-participants factors. Repeatability between the RECD and wRECD was assessed within each configuration using a repeated-measures ANOVA with test–retest, frequency, and coupler type as within-participants factors. Agreement between the RECD and wRECD was assessed for each coupling configuration using a repeated-measures ANOVA with RECD value, coupler type, and frequency as within-participants factors. Post hoc comparisons with Bonferroni corrections were used when appropriate to locate the frequencies at which differences occurred. A 3-dB criterion was defined to locate differences of clinical significance.Average absolute test–retest differences were within ±3 dB within each coupler and coupling configuration, and between the RECD and wRECD. The RECD and wRECD were in absolute agreement following HA-1-referenced transforms, with most frequencies agreeing within ±1 dB, except at 0.2 kHz for the earmold, and 0.2–0.25 kHz for the foam tip, where the average RECD exceeded the average wRECD by slightly >3 dB.Test–retest repeatability of the RECD (up to 8 kHz) and wRECD (up to 12.5 kHz) is acceptable and similar to previously reported data. The RECD and wRECD are referenced to different couplers, but can be rendered comparable with a simple transform, producing values that are in accordance with the ANSI S3.46-2013 standard.

Extra-High-Frequency Auditory Thresholds: Fine Structure, Reliability, Temporal Integration and Relation to Ear Canal Resonance

1985 ◽  
Vol 24 (2) ◽  
pp. 92-103 ◽  
Author(s):  
Kenneth R. Henry ◽  
Gary A. Fast ◽  
Hanh H. Nguyen ◽  
Marcie C. Paolinelli ◽  
Natalie M. Ayars
Keyword(s):  
Fine Structure ◽  
High Frequency ◽  
Temporal Integration ◽  
Ear Canal ◽  
Auditory Thresholds ◽  
Structure Reliability

scholarly journals Some Factors in the Activation of Crab Movement Receptors

1963 ◽  
Vol 40 (1) ◽  
pp. 157-169
Author(s):  
MARTIN MENDELSON
Keyword(s):  
Electrical Activity ◽  
Mechanical Stimulation ◽  
High Frequency ◽  
Action Potentials ◽  
High Concentration ◽  
Low Concentration ◽  
Receptor Cells ◽  
Considerable Distance ◽  
Distal Process ◽  
Long Duration

1. The activation of movement receptors in Pachygrapsus crassipes is examined. 2. Lengthening and shortening of the elastic strand of the propodite-dactylopodite (PD) organ are respectively the adequate stimuli for movement receptors responding uniquely either to closing or opening. These stimuli are also effective on the isolated PD organ. Twisting of the strand is without effect. 3. Intracellular records from the receptor cells show apparent intermittent generator potentials during effective stimulation. No electrical activity is observed at sub-threshold speeds or during movement in the inappropriate direction. The action potentials are initiated in the distal process at a considerable distance from the soma. 4. Nicotine applied to the PD organ in high concentration elicits spike discharges of high frequency and long duration; it has no effect when applied to the axons. Applied to the PD organ in low concentration it potentiates the effect of mechanical stimulation without itself eliciting spikes. 5. These findings are discussed in relation to the structure of the receptor endings and a mechanism is tentatively suggested to account for the unidirectional sensitivity.

scholarly journals Large, stable spikes exhibit differential broadening in excitatory and inhibitory neocortical boutons

2020 ◽  
Author(s):  
Andreas Ritzau-Jost ◽  
Timur Tsintsadze ◽  
Martin Krueger ◽  
Jonas Ader ◽  
Ingo Bechmann ◽  
...  
Keyword(s):  
Patch Clamp ◽  
High Frequency ◽  
Neurotransmitter Release ◽  
Action Potentials ◽  
Pyramidal Neurons ◽  
Cultured Neurons ◽  
Presynaptic Action ◽  
Presynaptic Spike ◽  
Fast Spiking

SUMMARYPresynaptic action potential spikes control neurotransmitter release and thus interneuronal communication. However, the properties and the dynamics of presynaptic spikes in the neocortex remain enigmatic because boutons in the neocortex are small and direct patch-clamp recordings have not been performed. Here we report direct recordings from boutons of neocortical pyramidal neurons and interneurons. Our data reveal rapid and large presynaptic action potentials in layer 5 neurons and fast-spiking interneurons reliably propagating into axon collaterals. For in-depth analyses we validate boutons of mature cultured neurons as models for excitatory neocortical boutons, demonstrating that the presynaptic spike amplitude was unaffected by potassium channels, homeostatic long-term plasticity, and high-frequency firing. In contrast to the stable amplitude, presynaptic spikes profoundly broadened for example during high-frequency firing in layer 5 pyramidal neurons but not in fast-spiking interneurons. Thus, our data demonstrate large presynaptic spikes and fundamental differences between excitatory and inhibitory boutons in the neocortex.

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