Sensitivity of porcine peripheral blood leukocytes to gamma irradiation in vivo, in vitro and ex vivo

2011 ◽  
Vol 87 (5) ◽  
pp. 491-498 ◽  
Author(s):  
Lenka Zarybnicka ◽  
Zuzana Sinkorova ◽  
Jiri Sinkora ◽  
Vaclav Blaha ◽  
Jirina Vavrova ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Peripheral Blood ◽  
Ex Vivo ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

Detection and characterization of apoptotic peripheral blood lymphocytes in human immunodeficiency virus infection and cancer chemotherapy by a novel flow immunocytometric method

Blood ◽  
1994 ◽  
Vol 83 (5) ◽  
pp. 1268-1277 ◽  
Author(s):  
M Carbonari ◽  
M Cibati ◽  
M Cherchi ◽  
D Sbarigia ◽  
AM Pesce ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cells ◽  
Hiv Infection ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Cell Counts ◽  
Immunodeficiency Virus

We have developed a quantitative and sensitive flow cytometric method for the detection of human apoptotic lymphocytes that, unlike previously described assays, allows their identification in mixed populations of peripheral blood leukocytes as well as their immunophenotyping. Apoptotic lymphocytes are identified on the basis of peculiar light scatter changes, reflecting their smaller size and their modified nucleus/cytoplasm organization, and of the decreased expression of surface CD45 molecules. Based on these criteria, apoptotic lymphocytes generated by exposure to ionizing radiation can be easily distinguished from viable cells and from necrotic lymphocytes generated by treatment with antibody and complement. Using this assay, we reappraised the phenomenon of the in vitro apoptosis of lymphocytes from patients with human immunodeficiency virus (HIV) infection. Lymphocytes from HIV patients, unlike those from normal HIV-negative subjects, undergo apoptosis upon simple in vitro culture. We found that the percentages of lymphocytes undergoing apoptosis were significantly higher in patients with low CD4 cell counts (< 400/microL) than in patients at earlier stages (> 400 CD4 cells/microL). However, phenotypic analysis disclosed that apoptotic lymphocytes generated in these cultures were mostly CD8+ T cells and CD19+ B cells. Thus, in contrast to what has been previously suggested, the phenomenon of in vitro lymphocyte apoptosis might not be pathogenetically related to the depletion of CD4+ T cells in acquired immunodeficiency syndrome. Nevertheless, it might represent an useful marker of disease progression. Our assay allows the analysis of unfractionated peripheral blood leukocytes and thus the identification of apoptotic lymphocytes circulating in vivo. Apoptotic lymphocytes could indeed be detected in the circulation of a patient with cancer shortly after high-dose cytotoxic chemotherapy. By contrast, no apoptotic lymphocytes could be detected in vivo in patients with early or advanced HIV infection.

scholarly journals EHV-1 gene63 is not essential for in vivo replication in horses and mice, nor does it affect reactivation in the horse: Short Communication

2001 ◽  
Vol 49 (4) ◽  
pp. 473-478
Author(s):  
J. Iqbal ◽  
A. S. Purewal ◽  
N. Edington
Keyword(s):  
Peripheral Blood ◽  
Acute Infection ◽  
Early Gene ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Equine Herpesvirus 1 ◽  
Nasal Swabs

The aim of this study was to investigate the role of immediate early gene (gene63) in the pathogenesis of equine herpesvirus 1 (EHV-1) acute and latent infections in equine and murine models. EHV-1 gene63 mutant virus (g63mut) along with EHV-1 (Ab4) was used for intracerebral and intranasal infection of 3 and 17-day-old mice. Both viruses were recovered at the same frequency from tissues after infection. Two Welsh ponies were infected via the intranasal route with each of the viruses. Acute infection was monitored by virus isolation from nasal swabs and peripheral blood leukocytes. Six weeks post infection, peripheral blood leukocytes were taken from ponies and in vitro reactivation was positive for both viruses. At autopsy, both viruses were isolated by co-cultivation from bronchial and submandibular lymph nodes. These findings indicate that the mutation of EHV-1 gene63 does not play a role in the establishment and reactivation from latency.

scholarly journals Detection and characterization of apoptotic peripheral blood lymphocytes in human immunodeficiency virus infection and cancer chemotherapy by a novel flow immunocytometric method

Blood ◽  
1994 ◽  
Vol 83 (5) ◽  
pp. 1268-1277 ◽  
Author(s):  
M Carbonari ◽  
M Cibati ◽  
M Cherchi ◽  
D Sbarigia ◽  
AM Pesce ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cells ◽  
Hiv Infection ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Cell Counts ◽  
Immunodeficiency Virus

Abstract We have developed a quantitative and sensitive flow cytometric method for the detection of human apoptotic lymphocytes that, unlike previously described assays, allows their identification in mixed populations of peripheral blood leukocytes as well as their immunophenotyping. Apoptotic lymphocytes are identified on the basis of peculiar light scatter changes, reflecting their smaller size and their modified nucleus/cytoplasm organization, and of the decreased expression of surface CD45 molecules. Based on these criteria, apoptotic lymphocytes generated by exposure to ionizing radiation can be easily distinguished from viable cells and from necrotic lymphocytes generated by treatment with antibody and complement. Using this assay, we reappraised the phenomenon of the in vitro apoptosis of lymphocytes from patients with human immunodeficiency virus (HIV) infection. Lymphocytes from HIV patients, unlike those from normal HIV-negative subjects, undergo apoptosis upon simple in vitro culture. We found that the percentages of lymphocytes undergoing apoptosis were significantly higher in patients with low CD4 cell counts (< 400/microL) than in patients at earlier stages (> 400 CD4 cells/microL). However, phenotypic analysis disclosed that apoptotic lymphocytes generated in these cultures were mostly CD8+ T cells and CD19+ B cells. Thus, in contrast to what has been previously suggested, the phenomenon of in vitro lymphocyte apoptosis might not be pathogenetically related to the depletion of CD4+ T cells in acquired immunodeficiency syndrome. Nevertheless, it might represent an useful marker of disease progression. Our assay allows the analysis of unfractionated peripheral blood leukocytes and thus the identification of apoptotic lymphocytes circulating in vivo. Apoptotic lymphocytes could indeed be detected in the circulation of a patient with cancer shortly after high-dose cytotoxic chemotherapy. By contrast, no apoptotic lymphocytes could be detected in vivo in patients with early or advanced HIV infection.

scholarly journals Cisplatin-DNA damage and repair in peripheral blood leukocytes in vivo and in vitro.

1992 ◽  
Vol 98 ◽  
pp. 53-59 ◽  
Author(s):  
M Dabholkar ◽  
L Bradshaw ◽  
R J Parker ◽  
I Gill ◽  
F Bostick-Bruton ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Dna Damage And Repair

Microtubular Formations of Endoplasmic Reticulum in Human Pathological Tissues

1967 ◽  
Vol 25 ◽  
pp. 172-173
Author(s):  
S. Chandra ◽  
S. Kuzmic ◽  
P. M. Brandt ◽  
J. H. Monroe
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Kidney Cells ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Cytoplasmic Structures ◽  
Pathological Tissues

A certain modification of the endoplasmic reticulum has been described in liver in severe hyperlipidaemia and in cells from a culture of peripheral blood leukocytes of a leukemic patient. Recently cytoplasmic structures presumed to be related to virus synthesis have been reported in monkey kidney cells infected with rubella virus and in the Sticker sarcoma of the dog. During our studies with fifteen cell lines originating from different human tissues, similar cytoplasmic structures have been observed and interpreted as modifications of the endoplasmic reticulum (ER).A number of cell lines have been established from either the peripheral blood leukocytes, lymph nodes, or spleens and thymuses of cancerous or noncancerous patients. Both biopsy and autopsy materials have been used. Tissues of in vitro or in vivo origin were processed for electron microscopy in the usual way.

scholarly journals ω-3 PUFAs and Resveratrol Differently Modulate Acute and Chronic Inflammatory Processes

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Joseph Schwager ◽  
Nathalie Richard ◽  
Christoph Riegger ◽  
Norman Salem
Keyword(s):  
Gene Expression ◽  
Chronic Inflammation ◽  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Acute Inflammation ◽  
Synergistic Effects ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

ω-3 PUFAs and polyphenols have multiple effects on inflammationin vivoandin vitro. The effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and resveratrol (RV) were investigated in LPS-stimulated peripheral blood leukocytes (PBLs) (i.e., acute inflammation) and IL-1βactivated human chondrocytes (i.e., chronic inflammation). Inflammatory mediators including chemokines, cytokines, interleukins, and PGE2were measured by multiplex analysis and gene expression was quantified by RT-PCR. In PBLs, RV decreased the secretion of PGE2, CCL5/RANTES, and CXCL8/IL-8 but increased IL-1β, IL-6, and IL-10. In contrast to RV,ω-3 PUFAs augmented the production of PGE2and CXCL8/IL-8. EPA and DHA similarly affected the pattern of inflammatory mediators. Combination of RV andω-3 PUFAs exerted synergistic effects on CCL5/RANTES and had additive effects on IL-6 or CXCL8/IL-8. Bothω-3 PUFAs and RV reduced catabolic gene expression (e.g., MMPs, ADAMTS-4, IL-1β, and IL-6) in activated chondrocytes. The data suggest thatω-3 PUFAs and RV differ in the regulation of acute inflammation of peripheral blood leukocytes but have common properties in modulating features related to chronic inflammation of chondrocytes.

scholarly journals Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro

2017 ◽  
Vol 51 (2) ◽  
pp. 39-45
Author(s):  
Milena Jankovic ◽  
Lada Zivkovic ◽  
Andrea Pirkovic ◽  
Dijana Topalovic ◽  
Dragana Dekanski ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Salvianolic Acid B ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Salvianolic Acid

scholarly journals Immune reactions in human filariasis

1978 ◽  
Vol 8 (2) ◽  
pp. 228-232 ◽  
Author(s):  
D Subrahmanyam ◽  
K Mehta ◽  
D S Nelson ◽  
Y V Rao ◽  
C K Rao
Keyword(s):  
Immunoglobulin G ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Wuchereria Bancrofti ◽  
Normal Subjects ◽  
Peripheral Blood Leukocytes ◽  
51Cr Release ◽  
Blood Leukocytes ◽  
Release Studies

Sera from cases of elephantiasis due to Wuchereria bancrofti infection promoted an intense adhesion of peripheral blood leukocytes to W. bancrofti microfilariae in vitro. A similar adhesion was also seen using sera from some normal persons living for several years in areas where filariasis is endemic. No such adhesion was evident with sera from microfilaria carriers or from normal subjects from nonendemic areas. The adhesion was complement independent and was associated with the immunoglobulin G fraction of serum. 51Cr release studies suggested the occurrence of cell-mediated cytotoxicity to W. bancrofti microfilariae in the presence of elephantiasis serum. Microfilariae of Litomosoides carinii could be isolated free of blood cells, from the blood of infected rats. In the presence of serum, or its immunoglobulin G fraction, from patients with elephantiasis, L. carinii microfilariae adhered to human peripheral blood leukocytes or rat spleen cells.

scholarly journals Differential Infectivity and Division ofToxoplasma gondii in Human Peripheral Blood Leukocytes

2000 ◽  
Vol 68 (8) ◽  
pp. 4822-4826 ◽  
Author(s):  
Jacqueline Y. Channon ◽  
Rosanne M. Seguin ◽  
Lloyd H. Kasper
Keyword(s):  
Dendritic Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Cell Types ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Differential Infectivity

ABSTRACT When tachyzoites were incubated with human peripheral blood leukocytes in vitro, more monocytes and dendritic cells than neutrophils or lymphocytes were infected. Although tachyzoites were able to divide in each of these cell types, monocytes and dendritic cells were more permissive to rapid tachyzoite division than neutrophils or lymphocytes.

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