Magnetic Field and the Initial Phase of the Protein Synthesis in Newborn and Adult Mice

1998 ◽  
Vol 17 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Györgyi Kubinyi ◽  
György Thuróczy ◽  
Hanna Sinay ◽  
László Szabó
1988 ◽  
Vol 119 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Stephen F. Kemp ◽  
Gregory L. Kearns ◽  
W. Grady Smith ◽  
M. Joycelyn Elders

Abstract. We have studied the effect of the somatomedin IGF-I on stimulation of synthesis of DNA, RNA, protein, and glycosaminoglycan in chick chondrocytes. Stimulation of RNA and protein synthesis was rapid, occurring within 10 min after addition of IGF-I. Stimulation of sulphation required a lag time of about 2 h, whether chains were initiated on native protein core or on the artificial acceptor 4-methyl-umbelliferyl-β-D-xyloside. DNA synthesis was not stimulated until after 10 h of exposure to IGF-I. Investigation of the rate of processing of proteoglycan core protein after pulselabelling chondrocytes with 35SO4 yielded data which were best described by a biexponential function. IGF-I had no effect on the t½ of the initial phase (16.44 min in the absence of IGF-I and 20.38 min in the presence of IGF-I). However, addition of IGF-I resulted in a decrease in the t½ of the terminal phase from 122.58 to 55.44 min, which may reflect an increase in synthesis in the enzymes necessary for polymerization and sulphation of proteoglycan.


2015 ◽  
Vol 81 (6) ◽  
Author(s):  
H. K. Moffatt

A one-dimensional model of magnetic relaxation in a pressureless low-resistivity plasma is considered. The initial two-component magnetic field $\boldsymbol{b}(\boldsymbol{x},t)$ is strongly helical, with non-uniform helicity density. The magnetic pressure gradient drives a velocity field that is dissipated by viscosity. Relaxation occurs in two phases. The first is a rapid initial phase in which the magnetic energy drops sharply and the magnetic pressure becomes approximately uniform; the helicity density is redistributed during this phase but remains non-uniform, and although the total helicity remains relatively constant, a Taylor state is not established. The second phase is one of slow diffusion, in which the velocity is weak, though still driven by persistent weak non-uniformity of magnetic pressure; during this phase, magnetic energy and helicity decay slowly and at constant ratio through the combined effects of pressure equalisation and finite resistivity. The density field, initially uniform, develops rapidly (in association with the magnetic field) during the initial phase, and continues to evolve, developing sharp maxima, throughout the diffusive stage. Finally it is proved that, if the resistivity is zero, the spatial mean $\langle (\boldsymbol{b}\boldsymbol{\cdot }\boldsymbol{{\rm\nabla}}\times \boldsymbol{b})/b^{2}\rangle$ is an invariant of the governing one-dimensional induction equation.


Genetics ◽  
1990 ◽  
Vol 124 (4) ◽  
pp. 949-955
Author(s):  
V K Mohl ◽  
G D Bennett ◽  
R H Finnell

Abstract Lymphocytes from adult mice bearing a known difference in genetic susceptibility to teratogen-induced exencephaly (SWV/SD, and DBA/2J) were evaluated for changes in protein synthesis following an in vivo heat treatment. Particular attention was paid to changes indicative of the heat shock response, a highly conserved response to environmental insult consisting of induction of a few, highly conserved proteins with simultaneous decreases in normal protein synthesis. The duration of heat shock protein induction in lymphocytes was found to be increased by 1 hr in the teratogen-sensitive SWV/SD strain as compared to the resistant DBA/2J strain. Densitometric analysis revealed a significant decrease in the relative synthesis of at least two non-heat shock proteins (36 kD and 45 kD) in the SWV/SD lymphocytes as compared to DBA/2J cells. The increased sensitivity of protein synthesis to hyperthermia in the SWV/SD lymphocytes were lost in the F1 progeny of reciprocal crosses between SWV/SD and DBA/2J mouse strains. Sensitivity to hyperthermia-induced exencephaly is recessive to resistance in these crosses. The relationship between altered protein synthesis and teratogen susceptibility is discussed.


1982 ◽  
Vol 94 (1) ◽  
pp. 11-20 ◽  
Author(s):  
J. de Koning ◽  
A. M. I. Tijssen ◽  
J. A. M. J. van Dieten ◽  
G. P. van Rees

Continuous exposure of hemi-pituitary glands from intact female rats to LH releasing hormone (LH-RH) in vitro displayed three phases in the pattern of LH release: during the first hour release of LH was low (first phase response), then it increased to a higher level during the second hour and remained constant during the next 2 h (second phase response), after which there was a refractoriness of LH release (third phase response). The initial phase response of pituitary glands from intact rats was blocked by EGTA (a Ca2+ chelator) but there was a small but significant increase in the rate of LH release during the second phase response. This increase could be prevented by inhibition of protein synthesis by cycloheximide. Cycloheximide and EGTA did not affect basal release of LH by glands from intact rats, neither did EGTA affect the high basal release of LH by glands from ovariectomized rats. However, the LH-RH-induced release of LH from pituitary glands of ovariectomized rats, which did not show the initial phase of low LH release, was completely suppressed by EGTA throughout a 4-h incubation period. The pattern of LH release stimulated by the combination of N6-monobutyryl cyclic AMP and theophylline (mbcAMP/theophylline) showed an initial phase of low LH release lasting 4 h after which it increased. The magnitude of the effect was small compared with the action of LH-RH. As it did with LH-RH, EGTA completely blocked the initial response, but allowed a small increase in the rate of LH release thereafter; this increase could also be blocked by inhibition of protein synthesis. Addition of EGTA to media during pretreatment of pituitary glands from intact rats with either LH-RH or mbcAMP/theophylline did not impair the facilitatory effect of these secretagogues on the responsiveness of the glands to subsequent exposure to LH-RH and cycloheximide and normal Ca2+ levels. The restoration of Ca2+ levels after withdrawal neither affected basal nor LH-RH-induced release of LH. Exclusion of Ca2+ from the media during a 6-h incubation of pituitary glands from intact rats with LH-RH prevented the glands from becoming refractory to subsequent stimulation by LH-RH, which occurs when normal Ca2+ concentrations are present. The results suggested that extracellular Ca2+ is obligatory for LH release and the induction of refractoriness by LH-RH. In contrast, that part of the action of LH-RH which is cyclic AMP-mediated and protein synthesis-dependent is not affected by withdrawal of extracellular Ca2+.


2021 ◽  
Vol 410 ◽  
pp. 3-8
Author(s):  
Viktor N. Pustovoit ◽  
Yuri V. Dolgachev

The formation energy of martensite nuclei in the austenite matrix is calculated. Nanoclusters with ferromagnetic order, which exist in austenite above the Curie temperature, reduce the formation energy of a critical martensite nucleation center when exposed to an external magnetic field. The data obtained are explained by the magnetic separation of the initial phase under the action of a magnetic field. A fluctuation increase in nanovolumes with a ferromagnetic order in austenite increases the energy in a atoms group of the matrix phase with a parallel spins arrangement. As a result, the nucleation rate of the martensite phase increases and the martensitic transformation proceeds more completely.


1972 ◽  
Vol 50 (3) ◽  
pp. 185-195
Author(s):  
Thomas F. Knott

It has been proposed by Enga and Bloom that combined electric and magnetic helical quadrupole fields may be used to perform a Stern–Gerlach experiment on charged particles. A detailed investigation shows that the longitudinal Lorentz force due to coupling of the transverse velocity of the particles to the transverse magnetic field produces an additional focusing effect which masks the Stern–Gerlach force in large regions of initial phase space. Consideration of uncompensated magnetic fields, produced by small random variations in conductor dimensions and location, shows that the tolerances required to preserve spin separation in the useful range of initial conditions are several orders of magnitude higher than can be achieved at this time.


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