Susceptibility of Bacteroides Species to Metronidazole during Treatment of Patients with Crohn's Disease and Healthy Individuals

ObjectiveAn inadequate host response to the intestinal microbiota likely contributes to the manifestation and progression of human inflammatory bowel disease (IBD). However, molecular approaches to unravelling the nature of the defective crosstalk and its consequences for intestinal metabolic and immunological networks are lacking. We assessed the mucosal transcript levels, splicing architecture and mucosa-attached microbial communities of patients with IBD to obtain a comprehensive view of the underlying, hitherto poorly characterised interactions, and how these are altered in IBD.DesignMucosal biopsies from Crohn's disease and patients with UC, disease controls and healthy individuals (n=63) were subjected to microbiome, transcriptome and splicing analysis, employing next-generation sequencing. The three data levels were integrated by different bioinformatic approaches, including systems biology-inspired network and pathway analysis.ResultsMicrobiota, host transcript levels and host splicing patterns were influenced most strongly by tissue differences, followed by the effect of inflammation. Both factors point towards a substantial disease-related alteration of metabolic processes. We also observed a strong enrichment of splicing events in inflamed tissues, accompanied by an alteration of the mucosa-attached bacterial taxa. Finally, we noted a striking uncoupling of the three molecular entities when moving from healthy individuals via disease controls to patients with IBD.ConclusionsOur results provide strong evidence that the interplay between microbiome and host transcriptome, which normally characterises a state of intestinal homeostasis, is drastically perturbed in Crohn's disease and UC. Consequently, integrating multiple OMICs levels appears to be a promising approach to further disentangle the complexity of IBD.

Download Full-text

Proteomic dataset: Profiling of cultivated Echerichia coli isolates from Crohn's disease patients and healthy individuals

Data in Brief ◽

10.1016/j.dib.2019.103734 ◽

2019 ◽

Vol 23 ◽

pp. 103734

Author(s):

Daria Rakitina ◽

Julia Baikova ◽

Olga Pobeguts ◽

Olga Bukato ◽

Ivan Butenko ◽

...

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Healthy Individuals ◽

Proteomic Dataset

Download Full-text

Increased Antibody Response to Fucosylated Oligosaccharides and Fucose-Carrying Bacteroides Species in Crohn’s Disease

Frontiers in Microbiology ◽

10.3389/fmicb.2020.01553 ◽

2020 ◽

Vol 11 ◽

Cited By ~ 1

Author(s):

Katharina Kappler ◽

Yi Lasanajak ◽

David F. Smith ◽

Lennart Opitz ◽

Thierry Hennet

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Antibody Response ◽

Bacteroides Species

Download Full-text

Relation between concentrations of metronidazole and Bacteroides spp in faeces of patients with Crohn's disease and healthy individuals.

Journal of Clinical Pathology ◽

10.1136/jcp.34.6.645 ◽

1981 ◽

Vol 34 (6) ◽

pp. 645-650 ◽

Cited By ~ 37

Author(s):

A Krook ◽

B Lindstrom ◽

J Kjellander ◽

G Jarnerot ◽

L Bodin

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Healthy Individuals

Download Full-text

Exploring the impact of Crohn’s disease on the intragastric environment of fasted adults

ADMET & DMPK ◽

10.5599/admet.846 ◽

2020 ◽

Vol 8 (2) ◽

pp. 122 ◽

Cited By ~ 1

Author(s):

Maria Vertzoni ◽

Christina Koulouri ◽

Androniki Poulou ◽

Konstantinos Goumas ◽

Christos Reppas

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Bile Acids ◽

Drug Product ◽

Statistical Investigation ◽

Healthy Individuals ◽

Sample Collection ◽

Pepsin Activity ◽

Potential Impact ◽

The Impact

<p class="ADMETabstracttext">We explored the potential impact of Crohn’s disease on the intragastric environment of fasted adults with a view to potential effects on intragastric performance of orally administered drugs in the fasted state. Data were collected from 15 healthy individuals and 15 patients with Crohn’s disease. All subjects remained fasted for at least 12h prior to gastroscopy. Intragastric resting volume and pH were measured upon aspiration. Osmolality, surface tension, pepsin activity, and content of six bile acids were measured within 4 months upon sample collection. Unlike intragastric volumes, intragastric osmolality was significantly increased by Crohn’s disease. However, mean osmolality value in patients was only slightly higher than in healthy individuals (293 vs. 257 mOsmol/kg, respectively), therefore, unlikely to affect intragastric drug product performance. Primarily due to the high variability of data in healthy individuals, the potential effects on intragastric pH and surface activity could not be evaluated on a statistical basis. However, based on average (mean and median) values, even if they are statistically significant, it seems unlikely to be of clinical significance. Inter-subject variability of pepsin activity, and total bile acids content was high in both the healthy and the patients’ groups. Statistical investigation of the potential impact of Crohn’s disease on these parameters requires prior designation of the minimum differences to be detected; such differences will determine the minimum sample size required of relevant investigations.</p>

Download Full-text

Antibodies anti-Saccharomyces cerevisiae (ASCA) do not differentiate Crohn's disease from celiac disease

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032010000300006 ◽

2010 ◽

Vol 47 (3) ◽

pp. 242-245 ◽

Cited By ~ 14

Author(s):

Lorete Maria da Silva Kotze ◽

Renato Mitsunori Nisihara ◽

Shirley Ramos da Rosa Utiyama ◽

Paulo Gustavo Kotze ◽

Petra Mirella Theiss ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Crohn’S Disease ◽

Celiac Disease ◽

Crohn's Disease ◽

Statistical Significance ◽

Gluten Free Diet ◽

Specific Marker ◽

Gluten Free ◽

Healthy Individuals ◽

Diet Compliance

CONTEXT: Anti-Saccharomyces cerevisiae antibodies (ASCA), considered serologic markers for Crohn's disease, were described in patients with celiac disease, disappearing after a gluten-free diet. OBJECTIVES: Evaluation of ASCA positivity in patients with Crohn's disease and celiac disease in relation to healthy individuals. METHODS: A total of 145 individuals were studied: 36 with Crohn's disease and 52 with celiac disease, that fulfilled the diagnostic criteria for both affections, and 57 healthy individuals for control. The celiac patients were divided as follow: group CeD I at diagnosis (n = 34), group CeD II with gluten-free diet compliance (n = 13) and group CeD III with transgressions to the diet (n = 5). ASCA IgA and IgG were determined by ELISA. RESULTS: With statistical significance, ASCA IgA were positive in Crohn's disease, celiac disease at diagnosis and celiac disease with diet transgressions; ASCA IgG in Crohn's disease and in all groups with celiac disease. CONCLUSIONS: The detection of ASCA in patients with celiac disease allows to suggest that ASCA is not a specific marker for Crohn's disease, but was associated with the inflammation of the small intestine. The increased levels of positive ASCA may be due to genetic factors and increased intestinal permeability.

Download Full-text

Association of Serum Neuron-Specific Enolase and C-Reactive Protein With Disease Location and Endoscopic Inflammation Degree in Patients With Crohn's Disease

Frontiers in Medicine ◽

10.3389/fmed.2021.663920 ◽

2021 ◽

Vol 8 ◽

Author(s):

Rui-Xia Yang ◽

Wei-Juan Song ◽

Zhi-Qi Wu ◽

Hemant Goyal ◽

Hua-Guo Xu

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Neuron Specific Enolase ◽

Routine Practice ◽

Daily Routine ◽

Healthy Individuals ◽

C Reactive Protein ◽

Intestinal Lesion ◽

Reactive Protein ◽

Severe Inflammation

Objective: The objective of this study was to explore the association between serum markers neuron-specific enolase (NSE) and C-reactive protein (CRP) with intestinal lesion location and degree of inflammation in patients with Crohn's disease (CD).Design: The levels of serum NSE, CRP, and fecal calprotectin (FC) in patients with CD were analyzed retrospectively. The severity of inflammatory lesions in the intestinal wall was accessed using the Simple Endoscopic Score for Crohn's disease (SES-CD).Results: The levels of NSE in patients with CD were higher than those of healthy individuals (14.87 vs. 12.68 ng/ml, P < 0.001). The levels of CRP in patients with CD were higher than those of healthy individuals (12.30 vs. 3.40 mg/l, P < 0.001). The FC levels in patients with CD were higher than those of patients with non-inflammatory bowel disease (1,143.90 vs. 114.21 μg/g, P < 0.05). The levels of NSE in CD with ileal lesions and simultaneous ileal and colon lesions were significantly higher than those in patients with CD with colonic lesions. However, the CRP was higher in patients with colonic lesions than those with ileal lesions. The levels of NSE in patients with severe inflammation were higher than those in patients with moderate inflammation (15.95 vs. 13.89 ng/ml, P < 0.05). Similarly, the NSE levels in patients with CD with severe inflammation were higher than those in patients with CD with mild inflammation (15.95 vs. 13.53 ng/mL, P < 0.05). The levels of CRP in severe inflammation were higher than those in moderate inflammation (29.80 vs. 19.60 mg/l, P < 0.05). In addition, the CRP levels in severe inflammation were higher than those in mild inflammation (29.80 vs. 5.86 mg/l, P < 0.05). ROC curve analysis showed that when NSE was combined with CRP for distinguishing between patients with CD and those without CD, sensitivity increased to 80.41%, specificity increased to 74.66%, and a highest AUC was equal to 0.843.Conclusion: Our study shows that serum NSE and CRP can be used to assess the severity of CD as well as the location of intestinal involvement. Therefore, NSE and CRP could be used as the non-invasive tests in detecting the location and severity of disease in patients with CD in daily routine practice.

Download Full-text

Metagenomic Analysis of Saliva Reveals Disease-Associated Microbiotas in Patients With Periodontitis and Crohn’s Disease-Associated Periodontitis

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.719411 ◽

2021 ◽

Vol 11 ◽

Author(s):

Boyang Sun ◽

Bingyao Liu ◽

Xiaojiao Gao ◽

Kai Xing ◽

Li Xie ◽

...

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Microbial Communities ◽

Antibiotic Resistance Genes ◽

Study Groups ◽

Metagenomic Analysis ◽

Metagenomic Sequencing ◽

Oral Diseases ◽

Healthy Individuals ◽

Illumina Hiseq

Patients with Crohn’s disease frequently develop oral health problems and show a higher prevalence of oral manifestations, such as dental caries and periodontitis, than healthy individuals do. In this study, a metagenomic analysis was carried out to characterize the salivary microbiota in patients with either periodontitis or Crohn’s disease-associated periodontitis. Saliva samples were collected from six patients with both Crohn’s disease and periodontitis (Cm group), six patients with periodontitis alone (Pm group), and six healthy individuals (Hm group). Genomic DNA was collected from these samples for high-throughput Illumina HiSeq metagenomic sequencing. The composition of the bacterial communities and their metabolic pathways and gene functions were characterized and compared among the three study groups. The salivary microbial communities were significantly different among the three groups, with Firmicutes, Actinobacteria, and Bacteroidetes showing the most significant differences. The Cm and Pm groups had higher abundances of Bacteroides fragilis, Prevotella baroniae, Prevotella enoeca, and Prevotella dentasini than the Hm group. The Cm and Pm groups also showed differences in their salivary microbial communities, in that the Cm group had relatively high abundances of Firmicutes and Proteobacteria, whereas the Pm group had relatively high abundances of Actinobacteria, Bacteroidetes, and Fusobacteria. In total, 34 Pm-associated (e.g., Fusobacteria and Corynebacterium matruchotii), 18 Cm-associated (e.g., Capnocytophaga and Streptococcus oralis), and 18 Hm-associated (e.g., Streptococcus and Bacillales) predominant microbial species were identified. Most genes were involved in carbohydrate and amino acid metabolism, with those of the Cm and Pm groups showing more similarity to one another but significant differences from those of the Hm group. Most of the antibiotic resistance genes were found in the Pm group. In conclusion, the salivary microbial community structure and abundance were distinct among patients with Crohn’s disease-associated periodontitis, patients with periodontitis, and healthy individuals. Further studies are needed to evaluate the potential value of these microbiota and microbiome differences in the clinical diagnosis and treatment of oral diseases.

Download Full-text