KEBUTUHAN STANDAR METODE UJI, BAHAN ACUAN, DAN KOMPETENSI SDM BERBASIS BIOTEKNOLOGI DI SEKTOR AGROINDUSTRI

Pengembangan standar berbasis bioteknologi pada sektor agroindustri dilakukan melalui peningkatan mutu laboratorium merupakan bagian infrastruktur standardisasi. Kondisi laboratorium bioteknologi di Indonesia yang diindikasikan di lapangan antara lain, metode uji, bahan acuan dan sebagian kompetensi SDM yang bekerja di laboratorium bioteknologi beragam. Penelitian ini dilakukan untuk memperkuat pengembangan metode uji, bahan acuan, dan standar SDM untuk mendukung pengembangan lingkup akreditasi laboratorium bioteknologi di Indonesia. Penelitian dilaksanakan dengan menggunakan desk study dan FGD. Berdasarkan hasil penelitian, dapat disimpulkan bahwa beberapa acuan standar Internasional dapat dijadikan sebagai acuan dalam penembangan Standar Nasional Indonesia terkait deteksi GMO dan spesifik DNA spesies antara lain, ISO 21569:2013 (Qualitative nucleic acid based methods), ISO 21570:2013 (Quantitative nucleic acid based methods), ISO 21571:2013 (Nucleic acid extraction), dan ISO 21572:2013 (Protein based methods), Selain itu, metode uji yang dikembangkan oleh European Union Laboratory for GM Food and Feed dan ENGL dapat digunakan sebagai rujukan dalam pengembangan SNI. Pengembangan bahan acuan pengujian GMO dan spesifik DNA dapat dirujuk dari Institute Materials and Measurements yang merupakan salah satu Joint Research Centre EURL-GMFF maupun LGC. InaCC di bawah Pusat Penelitian Biologi LIPI dapat dijadikan sebagai Bank RM sesuai skema yang dikonsepkan oleh KAN dilihat dari kesiapan dan kemampuannya. Beberapa rujukan standar SDM merujuk pada Peraturan bersama Kemendiknas dan Kepala BKN Nomor 02/V/PB/2010 dan Nomor 13 Tahun 2010 tanggal 6 Mei 2010 menjelaskan terkait dengan jabatan fungsional Pranata Laboratorium Pendidikan dan SKKNI bidang Jasa Pengujian Laboratorium merujuk poin MSL957014A mengenai Perform Moleculer Biology Tests Procedures.

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PLACE AND ROLE OF COMPETENT INFORMATION IN THE MANAGEMENT OF SCIENTIFIC AND TECHNOLOGICAL DEVELOPMENT

Naukovedenie ◽

10.31249/naukoved/2021.01.04 ◽

2021 ◽

pp. 123-138

Author(s):

Sergei Pyastolov ◽

Keyword(s):

European Union ◽

Technological Development ◽

Research Centre ◽

Joint Research ◽

Joint Research Centre ◽

Science Activity ◽

Scientific Organizations ◽

Special Space ◽

Scientific And Technological Development

VUCA world (Volatility, Uncertainty, Complexity, Ambiguity), according to experts of JRC (European Commission's Joint Research Centre), is now a special space that corrupts the integrity of science activity, limits thinking and vision of the situation by politicians. In fact, under such conditions, the only reliable resource for scientific organizations is a commercialization, and the payback is a condition of activity. These factors already pose a threat to global security. The mission orientation proposed by European Union experts is still seen as a rather weak alternative.

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Predicting Future Forest Ranges Using Array-based Geospatial Semantic Modelling

10.1101/009597 ◽

2014 ◽

Author(s):

Elise Mulder Osenga

Keyword(s):

Climate Change ◽

European Union ◽

Data Collection ◽

European Commission ◽

Research Centre ◽

The European Union ◽

Joint Research ◽

Joint Research Centre ◽

Semantic Modelling ◽

Impacts Of Climate Change

Studying the impacts of climate change requires looking at a multitude of variables across a broad range of sectors [1,2]. Information on the variables involved is often unevenly available or offers different uncertainties [3,4], and a lack of uniform terminology and methods further complicates the process of analysis, resulting in communication gaps when research enterprises span different sectors. For example, models designed by experts in one given discipline might assume conventions in language or oversimplify cross-disciplinary links in a way that is unfamiliar for scientists in another discipline. Geospatial Semantic Array Programming (GeoSemAP) offers the potential to move toward overcoming these challenges by promoting a uniform approach to data collection and sharing [5]. The Joint Research Centre of the European Commission has been exploring the use of geospatial semantics through a module in the PESETA II project (Projection of economic impacts of climate change in sectors of the European Union based on bottom-up analysis). This manuscript has been accepted for publication in IEEE Earthzine 2014 Vol. 7 Issue 2, 2nd quarter theme: Geospatial Semantic Array Programming. The definitive version will be published at: http://www.earthzine.org/

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Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

Pathogens ◽

10.3390/pathogens10020188 ◽

2021 ◽

Vol 10 (2) ◽

pp. 188

Author(s):

Tanja Hoffmann ◽

Andreas Hahn ◽

Jaco J. Verweij ◽

Gérard Leboulle ◽

Olfert Landt ◽

...

Keyword(s):

Nucleic Acid ◽

Real Time ◽

Real Time Pcr ◽

Acid Extraction ◽

Nucleic Acid Extraction ◽

Pcr Assay ◽

Bead Beating ◽

Stool Samples ◽

Pcr Assays ◽

Human Stool

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with group-, genus-, and species-specific real-time PCR assays targeting helminths and nonhelminth pathogens in human stool samples. From 25 different in-house and commercial helminth real-time PCR assays applied to 77 stool samples comprising 67 historic samples and 10 external quality assessment scheme samples positively tested for helminths, higher numbers of positive test results were observed after bead-beating-based nucleic acid extraction for 5/25 (20%) real-time PCR assays irrespective of specificity issues. Lower cycle threshold values were observed for one real-time PCR assay after the standard extraction scheme, and for four assays after the bead-beating-based scheme. Agreement between real-time PCR results after both nucleic acid extraction strategies according to Cohen’s kappa ranged from poor to almost perfect for the different assays. Varying agreement was observed in eight nonhelminth real-time PCR assays applied to 67 historic stool samples. The study indicates highly variable effects of harsh nucleic acid extraction approaches depending on the real-time PCR assay used.

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Forensic evaluation of two nucleic acid extraction systems and validation of a RT-qPCR protocol for identification of SARS-CoV-2 in post-mortem nasopharyngeal swabs

Forensic Science International ◽

10.1016/j.forsciint.2021.110775 ◽

2021 ◽

pp. 110775

Author(s):

Pedro A. Barrio ◽

Amparo Fernández-Rodríguez ◽

Pablo Martín ◽

Coro Fernández ◽

Lourdes Fernández ◽

...

Keyword(s):

Nucleic Acid ◽

Forensic Evaluation ◽

Acid Extraction ◽

Post Mortem ◽

Nucleic Acid Extraction

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Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCR

Viruses ◽

10.3390/v13040615 ◽

2021 ◽

Vol 13 (4) ◽

pp. 615

Author(s):

Allen Wing-Ho Chu ◽

Cyril Chik-Yan Yip ◽

Wan-Mui Chan ◽

Anthony Chin-Ki Ng ◽

Dream Lok-Sze Chan ◽

...

Keyword(s):

Nucleic Acid ◽

High Throughput ◽

Rna Extraction ◽

Nasopharyngeal Swab ◽

Acid Extraction ◽

Rt Pcr ◽

Nucleic Acid Extraction ◽

Suitable Alternative ◽

Pcr Inhibitors ◽

Pcr Targeting

SARS-CoV-2 RT-PCR with pooled specimens has been implemented during the COVID-19 pandemic as a cost- and manpower-saving strategy for large-scale testing. However, there is a paucity of data on the efficiency of different nucleic acid extraction platforms on pooled specimens. This study compared a novel automated high-throughput liquid-based RNA extraction (LRE) platform (PHASIFYTM) with a widely used magnetic bead-based total nucleic acid extraction (MBTE) platform (NucliSENS® easyMAG®). A total of 60 pools of nasopharyngeal swab and 60 pools of posterior oropharyngeal saliva specimens, each consisting of 1 SARS-CoV-2 positive and 9 SARS-CoV-2 negative specimens, were included for the comparison. Real-time RT-PCR targeting the SARS-CoV-2 RdRp/Hel gene was performed, and GAPDH RT-PCR was used to detect RT-PCR inhibitors. No significant differences were observed in the Ct values and overall RT-PCR positive rates between LRE and MBTE platforms (92.5% (111/120] vs 90% (108/120]), but there was a slightly higher positive rate for LRE (88.3% (53/60]) than MBTE (81.7% (49/60]) among pooled saliva. The automated LRE method is comparable to a standard MBTE method for the detection of SAR-CoV-2 in pooled specimens, providing a suitable alternative automated extraction platform. Furthermore, LRE may be better suited for pooled saliva specimens due to more efficient removal of RT-PCR inhibitors.

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Darina Blagoeva/Claudiu Pavel/Dominic Wittmer/Jacob Huisman/Francesco Pasimeni: Materials Dependencies for Dual-Use Technologies relevant to Europe’s Defence Sector. Luxemburg: Joint Research Centre of the European Commission, 2019

SIRIUS - Zeitschrift für Strategische Analysen ◽

10.1515/sirius-2020-3016 ◽

2020 ◽

Vol 4 (3) ◽

pp. 362-363

Author(s):

Jakob Kullik

Keyword(s):

European Commission ◽

Research Centre ◽

Dual Use ◽

Joint Research ◽

Joint Research Centre ◽

Defence Sector

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Synergistic use of electroosmotic flow and magnetic forces for nucleic acid extraction

The Analyst ◽

10.1039/c9an02191d ◽

2020 ◽

Vol 145 (6) ◽

pp. 2412-2419 ◽

Cited By ~ 1

Author(s):

Rachel N. Deraney ◽

Lindsay Schneider ◽

Anubhav Tripathi

Keyword(s):

Nucleic Acid ◽

Electric Field ◽

Microfluidic Chip ◽

Applied Electric Field ◽

Electroosmotic Flow ◽

Acid Extraction ◽

Nucleic Acid Extraction ◽

Magnetic Forces ◽

Extraction And Purification

NA extraction and purification utilitzing a microfluidic chip with applied electric field to induce electroosmotic flow opposite the magnetic NA-bound bead mix.

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