scholarly journals Seduhan daun dan buah jarak pagar (Jatropha curcas L.) untuk mengendalikan nematoda perongga akar Radopholus similis secara in vitro

2017 ◽  
Author(s):  
Ahmed Ibrahim Alrashid Yousif ◽  
Ankardiansyah Pandu Pradana ◽  
Muhammad Firdaus Oktafiyanto ◽  
Diana Putri ◽  
Abdul Munif

Radopholus similis is a nematode that infects many plantation crops. Infected plant roots by nematodes will show necrosis symptoms, then rot due to infection of secondary pathogens. Control of R. similis that is effective, environmentally friendly, and easy to be performed by the farmers is the key to success in reducing losses by this nematode. Extract of physic nut has been recognized to be effective in controlling some important pests, but there is currently no reports of its effectiveness in controlling R. similis. This study aimed to determine the effectiveness of leaves and fruit steeping of physic nut for in vitro control of R. similis. A total of 1:10 (w/v) leaves and fruit of physic nut were steepped, then filtered by a 500 mesh sieve. Steeping was then dripped into a suspension containing 40 R. similis. Twenty-four hours after treatment, the number of dead nematodes was observed. The leaves steeping of physic nut could kill up to 70% of R. similis. Mortality of R. similis treated with steeping of physic nut seeds reached 76.25%. Both steeping applications did not cause damage to the body of nematodes, thus it is presumably that the mortality was due to toxic compounds.

2014 ◽  
Vol 13 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Mahalakshmi Ravi ◽  
Eganathan Palanisami ◽  
Parida Ajay

2015 ◽  
Vol 176 (3) ◽  
pp. 892-902 ◽  
Author(s):  
Jefferson da Luz Costa ◽  
André Luís Lopes da Silva ◽  
Mário César Jucoski Bier ◽  
Gilvano Ebling Brondani ◽  
André Luiz Gollo ◽  
...  

2016 ◽  
Vol 7 (2) ◽  
pp. 114
Author(s):  
Titiek Yulianti

<p>Budi daya jarak pagar (Jatropha curcas) dengan sistem monokultur pada hamparan yang luas telah menim -bulkan ledakan suatu penyakit. Layu bakteri yang disebabkan oleh Ralstonia solanacearum merupakan salah satu penyakit utama jarak pagar dan ditemukan di beberapa daerah pengembangan. Gejala yang terlihat pada tanaman yang terinfeksi adalah layu dan daun menguning sebelum waktunya atau daun layu tanpa adanya perubahan warna dan masih melekat di batang. Jaringan pembuluh berubah warna kecokelatan. Akar utama dan sekunder busuk berwarna cokelat kehitaman. Pada tanaman yang terinfeksi cukup berat, daun-daunya akan gugur, bagian batang menjadi cokelat, dan akhirnya tanaman mati. Berdasar reaksi oksi-dasi sumber gula, biovar R. solanacearum yang diisolasi dari tanaman jarak pagar dari Malang, Jawa Timur mirip dengan biovar 5, sedangkan yang dari Pati, Jawa Tengah, berbeda dengan biovar standar yang ada. <br />Bakteri ini menginfeksi tomat, cabai merah, dan terong, tetapi tidak menginfeksi tembakau ataupun jagung. Observasi lapangan untuk mengetahui perkembangan layu bakteri pada tanaman jarak pagar dan penyebar-annya menunjukkan bahwa fluktuasi kejadian penyakit berkorelasi positif dengan curah hujan. Streptomycin sulfat atau kombinasi beberapa jenis antagonis merupakan cara pengendalian yang baik. Selain itu, mengoleskan CaCO3 pada luka akibat pemangkasan dapat mencegah penyakit berkembang lebih lanjut. Arah pene-litian ke depan untuk pengendalian penyakit ini adalah pengendalian terpadu yang menitik-beratkan kepada pertanian dan lingkungan yang keberlanjutan, misalnya penambahan antagonis, mikroorganisme berguna, bahan organik, serta pemupukan seimbang.</p><p> </p><p>Growing physic nut (Jatropha curcas) under monoculture system in large areas has generated disease out-break. Bacterial wilt caused by Ralstonia solanacearum is one of the major diseases found in several regions. The symptom of the infected plant is wilting and premature leaf yellowing or leaves wilting without changing colour and still attaching to the stem. The vascular tissues show a brown discoloration. The primary and secondary roots may become brown to black. Severe infection causes leaves of diseased plant to fall, the <br />stem to become brown and eventually death. Based on oxidation reaction of sugar source the biovar of R. solanacearum isolated from physic nut in Malang (East Java) was simil ar to biovar 5, but isolate from Pati, Central Java was different from the standard biovar. The pathogen infected tomato, red chili, and egg plant but not tobacco or maize. A field observation to determine the development of bacterial wilt in physic nut and its spread pattern demonstrated that disease fluctuation incidence was positively correlated to rainfall. <br />Streptomycin sulphate or combination of antagonists gave a good disease control. Furthermore, smearing CaCO3 on wound caused by prunning could prevent disease development. The best control measure is inte-gration of several control measures which encourage sustainable agriculture and environment, including the addition of antagonists, effective microorganism, organic matter, and balanced fertilizer.</p><p><br /><br /></p>


Nematology ◽  
2003 ◽  
Vol 5 (2) ◽  
pp. 205-212
Author(s):  
László Sági ◽  
Rony Swennen ◽  
Dirk De Waele ◽  
Willy Peumans ◽  
Els Van Damme ◽  
...  

AbstractThe motility and the chemotactic response towards plant roots of Radopholus similis, after treatment with novel types of lectins, were examined in vitro by analysing movement tracks on agar plates. Six plant lectins belonging to five different lectin families and a banana thaumatin-like protein (BanTLP) were included in the experiment. A 1% concentration of Phaseolus vulgaris agglutinin (PHA) had an adverse effect on the motility of R. similis females: 63% showed no or very little movement on agar plates compared to an average of 33% for other lectins and 3% for the control treatment. A 0.05% concentration of PHA still reduced the motility of R. similis females by 75%. Concanavalin A and wheat germ agglutinin did not alter the chemotactic response towards plant roots, despite binding of both lectins to R. similis. In contrast, Galanthus nivalis agglutinin (GNA) reduced orientated movement of R. similis towards plant roots. Subsequently, secretions of R. similis were stained with Coomassie Brilliant Blue R. Nematodes treated with GNA produced secretions less abundantly compared to the control treatment and BanTLP. The other lectins in the experiment had variable effects on secretion.


2018 ◽  
Vol 10 (2) ◽  
pp. 159 ◽  
Author(s):  
Abdullahi A. Imam ◽  
Amina Shehu ◽  
Ibrahim A. Khalil ◽  
Hadiza Abdullahi
Keyword(s):  

2012 ◽  
Vol 82 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Mauro Serafini ◽  
Giuseppa Morabito

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.


1976 ◽  
Vol 15 (05) ◽  
pp. 248-253
Author(s):  
A. K. Basu ◽  
S. K. Guha ◽  
B. N. Tandon ◽  
M. M. Gupta ◽  
M. ML. Rehani

SummaryThe conventional radioisotope scanner has been used as a whole body counter. The background index of the system is 10.9 counts per minute per ml of sodium iodide crystal. The sensitivity and derived sensitivity parameters have been evaluated and found to be suitable for clinical studies. The optimum parameters for a single detector at two positions above the lying subject have been obtained. It has been found that for the case of 131I measurement it is possible to assay a source located at any point in the body with coefficient of variation less than 5%. To add to the versatility, a fixed geometry for in-vitro counting of large samples has been obtained. The retention values obtained by the whole body counter have been found to correlate with those obtained by in-vitro assay of urine and stool after intravenous administration of 51Cr-albumin.


Author(s):  
Mohammed Ibrahim ◽  
Alaa Zaky ◽  
Mohsen Afouna ◽  
Ahmed Samy

Carrier erythrocytes are emerging as one of the most promising biological drug delivery systems investigated in recent decades. Beside its biocompatibility, biodegradability and ability to circulate throughout the body, it has the ability to perform extended release system of the drug for a long period. The ultimate goal of this study is to introduce a new carrier system for Salbutamol, maintaining suitable blood levels for a long time, as atrial to resolve the problems of nocturnal asthma medication Therefore in this work we study the effect of time, temperature as well as concentration on the loading of salbutamol in human erythrocytes to be used as systemic sustained release delivery system for this drug. After the loading process is performed the carrier erythrocytes were physically and cellulary characterized. Also, the in vitro release of salbutamol from carrier erythrocytes was studied over time interval. From the results it was found that, human erythrocytes have been successfully loaded with salbutamol using endocytosis method either at 25 Co or at 37 Co . The highest loaded amount was 3.5 mg/ml and 6.5 mg/ml respectively. Moreover, the percent of cells recovery is 90.7± 1.64%. Hematological parameters and osmotic fragility behavior of salbutamol loaded erythrocytes were similar that of native erythrocytes. Scanning electron microscopy demonstrated that the salbutamol loaded cells has moderate change in the morphology. Salbutamol releasing from carrier cell was 43% after 36 hours in phosphate buffer saline. The releasing pattern of the drug from loaded erythrocytes showed initial burst release in the first hour followed by a very slow release, obeying zero order kinetics. It concluded that salbutamol is successfully entrapped into erythrocytes with acceptable loading parameters and moderate morphological changes, this suggesting that erythrocytes can be used as prolonged release carrier for salbutamol.


Author(s):  
Noorzaid Muhamad ◽  
Syahirah Sazeli ◽  
Resni Mona ◽  
Jannathul Firdous

The anthelmintic resistance has limited the control of gastrointestinal nematodes of small ruminants and thus has awakened interest in the study of plants extract as a source of anthelmintics. These experiments were carried out to evaluate the in vitro efficacy of Jatrophacurcas latex extract against Haemonchuscontortus larval motility. To evaluate the larvicidal activity, H.contortus L3 were incubated with the extracts with varying concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL at 27°C for 48, 72 and 96 hrs. The results were subjected to the Kruskal-Wallis test (P less than 0.05). The extracts showed dose-dependent larvicidal effects. These results suggest that J.curcas can be used to control gastrointestinal nematodes of small ruminants.


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